Restriction map for
pFN21AB7643
(Linear) (Six-base) MAPSORT of: KIBB7643.seq Check: 3479 from: 1 to: 897
KIBB7643.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 897
Size: 360 537
AflIII A'CryG_T
Cuts at: 0 218 897
Size: 218 679
AleI CACnn'nnGTG
Cuts at: 0 428 637 694 897
Size: 428 209 57 203
Fragments arranged by size:
428 209 203 57
ApaLI G'TGCA_C
Cuts at: 0 715 897
Size: 715 182
ApoI r'AATT_y
Cuts at: 0 337 897
Size: 337 560
AvaI C'yCGr_G
Cuts at: 0 209 304 897
Size: 209 95 593
Fragments arranged by size:
593 209 95
BanII G_rGCy'C
Cuts at: 0 420 897
Size: 420 477
BglI GCCn_nnn'nGGC
Cuts at: 0 265 897
Size: 265 632
Bme1580I G_kGCm'C
Cuts at: 0 317 388 719 897
Size: 317 71 331 178
Fragments arranged by size:
331 317 178 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 897
Size: 40 857
BsaAI yAC'GTr
Cuts at: 0 219 897
Size: 219 678
BsaBI GATnn'nnATC
Cuts at: 0 7 897
Size: 7 890
BseYI C'CCAG_C
Cuts at: 0 836 897
Size: 836 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 711 897
Size: 633 78 186
Fragments arranged by size:
633 186 78
BsiEI CG_ry'CG
Cuts at: 0 414 897
Size: 414 483
BsiHKAI G_wGCw'C
Cuts at: 0 719 897
Size: 719 178
BsmI GAATG_Cn'
Cuts at: 0 446 897
Size: 446 451
BsmBI CGTCTCn'nnnn_
Cuts at: 0 730 897
Size: 730 167
Bsp1286I G_dGCh'C
Cuts at: 0 317 388 420 719 897
Size: 317 71 32 299 178
Fragments arranged by size:
317 299 178 71 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 360 897
Size: 260 100 537
Fragments arranged by size:
537 260 100
BsrBI CCG'CTC
Cuts at: 0 39 897
Size: 39 858
BsrDI GCAATG_nn'
Cuts at: 0 585 897
Size: 585 312
BsrGI T'GTAC_A
Cuts at: 0 377 530 897
Size: 377 153 367
Fragments arranged by size:
377 367 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 897
Size: 117 780
BtsI GCAGTG_nn'
Cuts at: 0 279 317 423 435 897
Size: 279 38 106 12 462
Fragments arranged by size:
462 279 106 38 12
EaeI y'GGCC_r
Cuts at: 0 816 897
Size: 816 81
EarI CTCTTCn'nnn_
Cuts at: 0 682 758 897
Size: 682 76 139
Fragments arranged by size:
682 139 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 897
Size: 40 857
EcoO109I rG'GnC_Cy
Cuts at: 0 669 707 897
Size: 669 38 190
Fragments arranged by size:
669 190 38
EcoRI G'AATT_C
Cuts at: 0 337 897
Size: 337 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 192 224 897
Size: 192 32 673
Fragments arranged by size:
673 192 32
HincII GTy'rAC
Cuts at: 0 480 897
Size: 480 417
MfeI C'AATT_G
Cuts at: 0 751 897
Size: 751 146
MscI TGG'CCA
Cuts at: 0 818 897
Size: 818 79
MslI CAynn'nnrTG
Cuts at: 0 428 565 637 694 897
Size: 428 137 72 57 203
Fragments arranged by size:
428 203 137 72 57
PasI CC'CwG_GG
Cuts at: 0 473 897
Size: 473 424
PflMI CCAn_nnn'nTGG
Cuts at: 0 891 897
Size: 891 6
PpuMI rG'GwC_Cy
Cuts at: 0 707 897
Size: 707 190
PstI C_TGCA'G
Cuts at: 0 400 430 897
Size: 400 30 467
Fragments arranged by size:
467 400 30
SanDI GG'GwC_CC
Cuts at: 0 707 897
Size: 707 190
SapI GCTCTTCn'nnn_
Cuts at: 0 682 897
Size: 682 215
ScaI AGT'ACT
Cuts at: 0 511 897
Size: 511 386
SexAI A'CCwGG_T
Cuts at: 0 151 897
Size: 151 746
SfcI C'TryA_G
Cuts at: 0 396 426 897
Size: 396 30 471
Fragments arranged by size:
471 396 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 265 897
Size: 265 632
SmaI CCC'GGG
Cuts at: 0 306 897
Size: 306 591
SmlI C'TyrA_G
Cuts at: 0 209 897
Size: 209 688
StyI C'CwwG_G
Cuts at: 0 864 897
Size: 864 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 428 645 897
Size: 31 397 217 252
Fragments arranged by size:
397 252 217 31
TatI w'GTAC_w
Cuts at: 0 377 509 530 897
Size: 377 132 21 367
Fragments arranged by size:
377 367 132 21
XhoI C'TCGA_G
Cuts at: 0 209 897
Size: 209 688
XmaI C'CCGG_G
Cuts at: 0 304 897
Size: 304 593
XmnI GAAnn'nnTTC
Cuts at: 0 511 897
Size: 511 386
Enzymes that do cut:
AarI AflIII AleI ApaLI ApoI AvaI BanII BglI
Bme1580I BpmI BsaAI BsaBI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII
MfeI MscI MslI PasI PflMI PpuMI PstI SanDI
SapI ScaI SexAI SfcI SfiI SmaI SmlI StyI
TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BsiWI BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MmeI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SbfI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI ZraI