Restriction map for
pFN21AB7841
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BalI BanII BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NcoI
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 505 GCGG CCGC(-3/-1)
AfaI 556 GTAC GT^AC
AgeI 1396 ACCGGT A^CCGGT
Alw44I 46 GTGCAC G^TGCAC
AlwNI 82 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 46 GTGCAC G^TGCAC
AseI 1369 ATTAAT AT^TAAT
AsnI 1369 ATTAAT AT^TAAT
Asp700I 976 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1385 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 69 CTCGGG C^[CT]CG[GA]G
BamHI 620 GGATCC G^GATCC
BbeI 91 GGCGCC GGCGC^C
BclI 1419 TGATCA T^GATCA
BfaI 683 CTAG C^TAG
BglI 65 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1396 ACCGGT [AT]^CCGG[AT]
BsmAI 428 GAGAC GTCTC(1/5)
BsoBI 69 CTCGGG C^[CT]CG[GA]G
BspHI 768 TCATGA T^CATGA
BstYI 620 GGATCC [GA]^GATC[CT]
DraIII 813 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1133 GAATTC G^AATTC
EcoT14I 261 CCAAGG C^C[AT][AT]GG
FbaI 1419 TGATCA T^GATCA
HgaI 58 GACGC GACGC(5/10)
HincII 628 GTTGAC GT[CT]^[GA]AC
HindII 628 GTTGAC GT[CT]^[GA]AC
KasI 91 GGCGCC G^GCGCC
MaeI 683 CTAG C^TAG
MaeII 214 ACGT A^CGT
MaeIII 216 GTCAC ^GT[ACGT]AC
MflI 620 GGATCC [GA]^GATC[CT]
NaeI 94 GCCGGC GCC^GGC
NarI 91 GGCGCC GG^CGCC
NdeI 272 CATATG CA^TATG
NgoMIV 94 GCCGGC G^CCGGC
NheI 682 GCTAGC G^CTAGC
PflFI 1385 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 160 CCATTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1396 ACCGGT A^CCGGT
PleI 34 GAGTC GAGTC(4/5)
PshBI 1369 ATTAAT AT^TAAT
RcaI 768 TCATGA T^CATGA
RsaI 556 GTAC GT^AC
SfoI 91 GGCGCC GGC^GCC
SgrAI 93 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 13 CTCAAG C^T[CT][GA]AG
StyI 261 CCAAGG C^C[AT][AT]GG
Tsp45I 216 GTCAC ^GT[GC]AC
Tth111I 1385 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 160 CCATTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 152 CCAGCCTTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 620 GGATCC [GA]^GATC[CT]
XmnI 976 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 58 GACGCC G[GA]^CG[CT]C
91 GGCGCC
AvaII 522 GGACC G^G[AT]CC
1390 GGTCC
BcnI 38 CCGGG CC^[GC]GG
1197 CCGGG
BsaHI 58 GACGCC G[GA]^CG[CT]C
91 GGCGCC
BsgI 716 GTGCAG GTGCAG(16/14)
1121 GTGCAG
BsiYI 156 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
160 CCATTGGATGG
BslI 156 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
160 CCATTGGATGG
BsrDI 125 CATTGC GCAATG(2/0)
1014 GCAATG
BsrFI 94 GCCGGC [GA]^CCGG[CT]
1396 ACCGGT
BsrI 87 ACTGG ACTGG(1/-1)
495 CCAGT
BstAPI 1014 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1243 GCAAGCAGTGC
Cfr10I 94 GCCGGC [GA]^CCGG[CT]
1396 ACCGGT
DraII 521 GGGACCC [GA]G^G[ACGT]CC[CT]
1389 GGGTCCC
DsaI 583 CCACGG C^C[GA][CT]GG
826 CCGTGG
EarI 1038 CTCTTC CTCTTC(1/4)
756 GAAGAG
EcoO109I 521 GGGACCC [GA]G^G[ACGT]CC[CT]
1389 GGGTCCC
HaeIII 3 GGCC GG^CC
830 GGCC
Hin1I 58 GACGCC G[GA]^CG[CT]C
91 GGCGCC
Ksp632I 1038 CTCTTC CTCTTC(1/4)
756 GAAGAG
MslI 592 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1010 CACAGCAATG
NciI 38 CCGGG CC^[GC]GG
1197 CCGGG
PpuMI 521 GGGACCC [GA]G^G[AT]CC[CT]
1389 GGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AluI AlwI ApoI AspHI
BanI BbvI BmyI BsaJI BsiHKAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I