(Circular) (Six-base) MAPSORT of: pFN21AB7841.seq Check: 8713 from: 1 to: 6217 pFN21AB7841 6217 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2979 3179 4280 5664 5932 1632 Size: 1347 200 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1347 1101 268 200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 3401 5048 3401 Size: 1647 4570 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 2601 1166 Size: 1435 4782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3239 3242 3261 1184 Size: 2055 3 19 4140 Fragments arranged by size: 4140 2055 19 3 AlwNI CAG_nnn'CTG Cuts at: 2092 3960 5532 2092 Size: 1868 1572 2777 Fragments arranged by size: 2777 1868 1572 ApaLI G'TGCA_C Cuts at: 2051 4274 5430 2051 Size: 2223 1156 2838 Fragments arranged by size: 2838 2223 1156 ApoI r'AATT_y Cuts at: 1493 2283 2516 2579 2985 3117 3138 3439 3526 Size: 790 233 63 406 132 21 301 87 Cuts at: 3526 3752 3904 6013 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 406 301 233 226 152 132 87 63 21 AseI AT'TA_AT Cuts at: 160 1032 3375 4771 160 Size: 872 2343 1396 1606 Fragments arranged by size: 2343 1606 1396 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2074 3542 1870 Size: 73 131 1468 4545 Fragments arranged by size: 4545 1468 131 73 BamHI G'GATC_C Cuts at: 2625 3547 3914 2625 Size: 922 367 4928 Fragments arranged by size: 4928 922 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 3077 3277 3538 4993 Size: 325 217 627 309 981 200 261 1455 Cuts at: 4993 618 Size: 1842 Fragments arranged by size: 1842 1455 981 627 325 309 261 217 200 BanII G_rGCy'C Cuts at: 1391 3536 1391 Size: 2145 4072 BbeI G_GCGC'C Cuts at: 2100 2100 Size: 6217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 3424 1548 Size: 1876 4341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2076 4828 136 Size: 107 122 71 631 605 404 2752 1525 Fragments arranged by size: 2752 1525 631 605 404 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2055 2619 3280 4278 5434 1790 Size: 265 564 661 998 1156 2573 Fragments arranged by size: 2573 1156 998 661 564 265 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 3948 4906 Size: 416 53 83 399 24 685 1846 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1846 1753 958 685 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2691 1056 Size: 1635 4582 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2787 2787 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4877 1239 Size: 3638 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2003 4342 5207 5505 2003 Size: 2339 865 298 2715 Fragments arranged by size: 2715 2339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2097 4024 4406 Size: 53 83 186 936 531 33 1927 382 Cuts at: 4406 5041 275 Size: 635 1451 Fragments arranged by size: 1927 1451 936 635 531 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 3401 4650 5048 5322 5469 5880 3401 Size: 1249 398 274 147 411 3738 Fragments arranged by size: 3738 1249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 2530 2875 5420 5916 2530 Size: 345 2545 496 2831 Fragments arranged by size: 2831 2545 496 345 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 3147 1163 Size: 573 1006 405 4233 Fragments arranged by size: 4233 1006 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 2055 2187 2909 4278 4363 5434 2019 Size: 36 132 722 1369 85 1071 2802 Fragments arranged by size: 2802 1369 1071 722 132 85 36 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2055 2187 2619 2909 3280 3536 Size: 399 229 36 132 432 290 371 256 Cuts at: 3536 4278 4363 5434 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 371 290 256 229 132 85 36 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 2773 4106 5836 2773 Size: 1333 1730 3154 Fragments arranged by size: 3154 1730 1333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 3026 4712 4886 65 Size: 1183 881 897 1686 174 1396 Fragments arranged by size: 1686 1396 1183 897 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2099 3401 4861 5048 1700 Size: 110 289 1302 1460 187 2869 Fragments arranged by size: 2869 1460 1302 289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 3025 3254 794 Size: 2231 229 3757 Fragments arranged by size: 3757 2231 229 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2625 3547 3914 4299 4316 5768 5854 5866 Size: 653 922 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 922 653 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2588 2831 513 Size: 552 939 584 243 3899 Fragments arranged by size: 3899 939 584 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2563 2920 3259 3277 3733 4527 4547 2563 Size: 357 339 18 456 794 20 4233 Fragments arranged by size: 4233 794 456 357 339 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2448 2483 529 Size: 35 1884 35 4263 Fragments arranged by size: 4263 1884 35 35 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DraIII CAC_nnn'GTG Cuts at: 2823 2823 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182 Size: 54 1425 50 275 18 1834 889 1629 Cuts at: 6182 8 Size: 43 Fragments arranged by size: 1834 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2756 3049 4147 1353 Size: 1403 293 1098 3423 Fragments arranged by size: 3423 1403 1098 293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2979 3179 4280 4877 5664 5932 1239 Size: 393 1347 200 1101 597 787 268 1524 Fragments arranged by size: 1524 1347 1101 787 597 393 268 200 EcoO109I rG'GnC_Cy Cuts at: 2527 3395 2527 Size: 868 5349 EcoRI G'AATT_C Cuts at: 3138 3526 3904 3138 Size: 388 378 5451 Fragments arranged by size: 5451 388 378 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2100 2106 2882 5364 1678 Size: 422 6 776 2482 2531 Fragments arranged by size: 2531 2482 776 422 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373 Size: 32 135 32 3286 32 42 32 2626 Fragments arranged by size: 3286 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2635 3561 3807 669 Size: 988 281 697 926 246 3079 Fragments arranged by size: 3079 988 926 697 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2096 2096 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2601 3019 4175 4534 4693 518 Size: 648 1435 418 1156 359 159 2042 Fragments arranged by size: 2042 1435 1156 648 418 359 159 MspA1I CmG'CkG Cuts at: 1606 3633 4310 5458 5703 1606 Size: 2027 677 1148 245 2120 Fragments arranged by size: 2120 2027 1148 677 245 NaeI GCC'GGC Cuts at: 2101 2101 Size: 6217 NarI GG'CG_CC Cuts at: 2097 2097 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 2278 387 Size: 1891 4326 NgoMIV G'CCGG_C Cuts at: 2099 2099 Size: 6217 NheI G'CTAG_C Cuts at: 1052 2687 1052 Size: 1635 4582 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2171 1108 Size: 902 161 5154 Fragments arranged by size: 5154 902 161 PfoI T'CCnGG_A Cuts at: 3006 3006 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 3395 2527 Size: 868 5349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PstI C_TGCA'G Cuts at: 830 3569 830 Size: 2739 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 2527 3395 2527 Size: 868 5349 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 3565 4700 5381 5572 826 Size: 219 2520 1135 681 191 1471 Fragments arranged by size: 2520 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2098 2098 Size: 6217 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 2099 1700 Size: 399 5818 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2018 4321 5222 5484 820 Size: 197 926 75 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 262 197 75 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2266 3656 513 Size: 552 939 262 1390 3074 Fragments arranged by size: 3074 1390 939 552 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2002 2202 2300 2760 4244 4261 4414 Size: 1255 422 200 98 460 1484 17 153 Cuts at: 4414 4599 5044 325 Size: 185 445 1498 Fragments arranged by size: 1498 1484 1255 460 445 422 200 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4463 6175 96 Size: 274 80 33 51 1443 2486 1712 138 Fragments arranged by size: 2486 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2296 2328 6177 6209 2296 Size: 32 3849 32 2304 Fragments arranged by size: 3849 2304 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3393 1316 Size: 2077 4140 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2985 4346 2985 Size: 1361 4856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI