(Circular) (Six-base) MAPSORT of: pFN21ASDA1028.seq Check: 5712 from: 1 to: 6235 pFN21ASDA1028 6235 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3239 3239 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2127 3578 1937 Size: 190 1451 4594 Fragments arranged by size: 4594 1451 190 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2942 3135 3339 4298 5682 5950 1632 Size: 1310 193 204 959 1384 268 1917 Fragments arranged by size: 1917 1384 1310 959 268 204 193 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 3522 5066 3522 Size: 1544 4691 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 3978 5550 3978 Size: 1572 4663 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 3092 3544 3770 3922 6031 1493 Size: 1599 452 226 152 2109 1697 Fragments arranged by size: 2109 1697 1599 452 226 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 3560 1870 Size: 73 1617 4545 Fragments arranged by size: 4545 1617 73 BamHI G'GATC_C Cuts at: 2593 2977 3016 3440 3565 3932 2593 Size: 384 39 424 125 367 4896 Fragments arranged by size: 4896 424 384 367 125 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 3205 3275 3322 3556 Size: 325 217 627 324 1094 70 47 234 Cuts at: 3556 5011 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 324 234 217 70 47 BanII G_rGCy'C Cuts at: 1391 2626 3494 3554 1391 Size: 1235 868 60 4072 Fragments arranged by size: 4072 1235 868 60 BbeI G_GCGC'C Cuts at: 3209 3209 Size: 6235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2447 4127 5337 2447 Size: 1680 1210 3345 Fragments arranged by size: 3345 1680 1210 BclI T'GATC_A Cuts at: 1548 2770 1548 Size: 1222 5013 BfrBI ATG'CAT Cuts at: 2171 2171 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2396 6230 2396 Size: 3834 2401 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 3278 4296 5452 1790 Size: 1488 1018 1156 2573 Fragments arranged by size: 2573 1488 1156 1018 BmgBI CAC'GTC Cuts at: 1318 2125 1318 Size: 807 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2830 3966 4924 Size: 416 53 83 399 24 1413 1136 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1413 1136 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2610 3292 3388 4895 1239 Size: 1371 682 96 1507 2579 Fragments arranged by size: 2579 1507 1371 682 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4898 882 Size: 617 3399 2219 Fragments arranged by size: 3399 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3532 4026 493 Size: 711 2328 494 2702 Fragments arranged by size: 2702 2328 711 494 BsaBI GATnn'nnATC Cuts at: 2592 6229 2592 Size: 3637 2598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3206 4042 4424 5059 Size: 53 83 186 936 1673 836 382 635 Cuts at: 5059 275 Size: 1451 Fragments arranged by size: 1673 1451 936 836 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3278 3522 4668 5066 5340 5487 5898 3278 Size: 244 1146 398 274 147 411 3615 Fragments arranged by size: 3615 1146 411 398 274 244 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 3160 3190 1178 Size: 30 165 30 660 30 1067 30 4223 Fragments arranged by size: 4223 1067 660 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2559 1186 Size: 1373 4862 BseYI C'CCAG_C Cuts at: 3257 5438 5934 3257 Size: 2181 496 3558 Fragments arranged by size: 3558 2181 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5662 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3461 3494 4296 4381 5452 3461 Size: 33 802 85 1071 4244 Fragments arranged by size: 4244 1071 802 85 33 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 BsmBI CGTCTCn'nnnn_ Cuts at: 3486 3486 Size: 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2626 3278 3461 3494 3554 4296 4381 Size: 399 836 652 183 33 60 742 85 Cuts at: 4381 5452 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 836 742 652 399 183 85 60 33 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 2767 4124 5854 2767 Size: 1357 1730 3148 Fragments arranged by size: 3148 1730 1357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3239 3590 844 Size: 2395 351 3489 Fragments arranged by size: 3489 2395 351 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2065 4122 1394 Size: 429 236 6 2057 3507 Fragments arranged by size: 3507 2057 429 236 6 BsrDI GCAATG_nn' Cuts at: 65 1248 2261 4730 4904 65 Size: 1183 1013 2469 174 1396 Fragments arranged by size: 2469 1396 1183 1013 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3062 3522 4879 5066 1700 Size: 110 1252 460 1357 187 2869 Fragments arranged by size: 2869 1357 1252 460 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 3010 794 Size: 2216 4019 BstBI TT'CG_AA Cuts at: 2007 5039 2007 Size: 3032 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2396 2593 2977 3016 3440 3565 3932 4317 Size: 424 197 384 39 424 125 367 385 Cuts at: 4317 4334 5786 5872 5884 6230 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 424 424 385 384 367 346 197 125 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2973 3341 513 Size: 552 957 951 368 3407 Fragments arranged by size: 3407 957 951 552 368 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 3751 4545 4565 3751 Size: 794 20 5421 Fragments arranged by size: 5421 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200 Size: 54 1425 50 275 18 1852 889 1629 Cuts at: 6200 8 Size: 43 Fragments arranged by size: 1852 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 4165 1353 Size: 2125 687 3423 Fragments arranged by size: 3423 2125 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 2155 4822 5196 5342 387 Size: 1064 138 480 86 2667 374 146 1280 Fragments arranged by size: 2667 1280 1064 480 374 146 138 86 EcoICRI GAG'CTC Cuts at: 3492 3492 Size: 6235 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2610 2942 3135 3292 3339 3388 4298 Size: 393 978 332 193 157 47 49 910 Cuts at: 4298 4895 5682 5950 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 978 910 787 597 393 332 268 193 157 49 47 EcoNI CCTnn'n_nnAGG Cuts at: 3317 3317 Size: 6235 EcoO109I rG'GnC_Cy Cuts at: 2510 3034 3350 2510 Size: 524 316 5395 Fragments arranged by size: 5395 524 316 EcoRI G'AATT_C Cuts at: 3092 3544 3922 3092 Size: 452 378 5405 Fragments arranged by size: 5405 452 378 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 3209 5382 1678 Size: 1531 2173 2531 Fragments arranged by size: 2531 2173 1531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2962 2994 4876 4908 4950 Size: 32 135 32 1390 32 1882 32 42 Cuts at: 4950 4982 1373 Size: 32 2626 Fragments arranged by size: 2626 1882 1390 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 3579 3825 669 Size: 988 281 768 873 246 3079 Fragments arranged by size: 3079 988 873 768 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 3205 3205 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3005 5349 5533 1305 Size: 1700 2344 184 2007 Fragments arranged by size: 2344 2007 1700 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2250 4193 4552 4711 518 Size: 648 1084 1943 359 159 2042 Fragments arranged by size: 2042 1943 1084 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3651 4328 5476 5721 1606 Size: 2045 677 1148 245 2120 Fragments arranged by size: 2120 2045 1148 677 245 NaeI GCC'GGC Cuts at: 3064 3064 Size: 6235 NarI GG'CG_CC Cuts at: 3206 3206 Size: 6235 NcoI C'CATG_G Cuts at: 513 1065 2022 2973 513 Size: 552 957 951 3775 Fragments arranged by size: 3775 957 951 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NgoMIV G'CCGG_C Cuts at: 3062 3062 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NsiI A_TGCA'T Cuts at: 2173 2173 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6235 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2510 3034 2510 Size: 524 5711 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PstI C_TGCA'G Cuts at: 830 2812 3015 3587 830 Size: 1982 203 572 3478 Fragments arranged by size: 3478 1982 572 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 SacI G_AGCT'C Cuts at: 3494 3494 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SexAI A'CCwGG_T Cuts at: 3318 3318 Size: 6235 SfcI C'TryA_G Cuts at: 826 1045 2808 3011 3583 4718 5399 5590 826 Size: 219 1763 203 572 1135 681 191 1471 Fragments arranged by size: 1763 1471 1135 681 572 219 203 191 SfoI GGC'GCC Cuts at: 3207 3207 Size: 6235 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4339 5240 5502 820 Size: 197 926 2396 901 262 1553 Fragments arranged by size: 2396 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3532 4026 493 Size: 3039 494 2702 Fragments arranged by size: 3039 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2022 2973 3674 513 Size: 552 957 951 701 3074 Fragments arranged by size: 3074 957 951 701 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 3245 3519 4262 4279 4432 4617 Size: 1255 869 796 274 743 17 153 185 Cuts at: 4617 5062 325 Size: 445 1498 Fragments arranged by size: 1498 1255 869 796 743 445 274 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 4481 6193 Size: 274 80 33 51 1443 930 1574 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 1712 1574 1443 930 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2776 2808 3160 3192 3235 3267 6195 6227 2776 Size: 32 352 32 43 32 2928 32 2784 Fragments arranged by size: 2928 2784 352 43 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2429 3461 2429 Size: 1032 5203 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 2273 4364 2273 Size: 2091 4144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI