(Circular) (Six-base) MAPSORT of: pFN21AB9536.seq Check: 1853 from: 1 to: 5911 pFN21AB9536 5911 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 1937 Size: 1317 4594 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1998 2479 3974 5358 5626 1632 Size: 366 481 1495 1384 268 1917 Fragments arranged by size: 1917 1495 1384 481 366 268 AfeI AGC'GCT Cuts at: 2343 2343 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 2387 4810 5787 1203 Size: 1184 2423 977 1327 Fragments arranged by size: 2423 1327 1184 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 2538 2790 2968 3126 1166 Size: 1372 252 178 158 3951 Fragments arranged by size: 3951 1372 252 178 158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 3654 5226 3654 Size: 1572 4339 ApaLI G'TGCA_C Cuts at: 2771 3968 5124 2771 Size: 1197 1156 3558 Fragments arranged by size: 3558 1197 1156 ApoI r'AATT_y Cuts at: 1493 2378 2407 2943 3220 3446 3598 5707 1493 Size: 885 29 536 277 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 536 277 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2561 3236 1870 Size: 73 618 675 4545 Fragments arranged by size: 4545 675 618 73 AvrII C'CTAG_G Cuts at: 2998 2998 Size: 5911 BamHI G'GATC_C Cuts at: 2277 3241 3608 2277 Size: 964 367 4580 Fragments arranged by size: 4580 964 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2729 2856 3232 4687 618 Size: 325 217 627 942 127 376 1455 1842 Fragments arranged by size: 1842 1455 942 627 376 325 217 127 BanII G_rGCy'C Cuts at: 1391 3230 1391 Size: 1839 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4940 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BfrBI ATG'CAT Cuts at: 2101 2101 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2454 4522 136 Size: 107 122 71 631 605 782 2068 1525 Fragments arranged by size: 2068 1525 782 631 605 122 107 71 BglII A'GATC_T Cuts at: 2257 2593 2923 5906 2257 Size: 336 330 2983 2262 Fragments arranged by size: 2983 2262 336 330 BlpI GC'TnA_GC Cuts at: 3173 3328 3173 Size: 155 5756 Bme1580I G_kGCm'C Cuts at: 1790 2775 3972 5128 1790 Size: 985 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 985 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2211 2631 2715 Size: 416 53 83 399 24 794 420 84 Cuts at: 2715 3642 4600 442 Size: 927 958 1753 Fragments arranged by size: 1753 958 927 794 420 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4571 1239 Size: 3332 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3188 4036 4901 5199 3188 Size: 848 865 298 3900 Fragments arranged by size: 3900 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3718 4100 4735 275 Size: 53 83 186 936 2185 382 635 1451 Fragments arranged by size: 2185 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3059 1186 Size: 1873 4038 BseYI C'CCAG_C Cuts at: 5114 5610 5114 Size: 496 5415 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2775 3972 4057 5128 2775 Size: 1197 85 1071 3558 Fragments arranged by size: 3558 1197 1071 85 BsmI GAATG_Cn' Cuts at: 2150 3422 3515 2150 Size: 1272 93 4546 Fragments arranged by size: 4546 1272 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2775 3230 3972 4057 5128 1391 Size: 399 985 455 742 85 1071 2174 Fragments arranged by size: 2174 1071 985 742 455 399 85 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 3266 844 Size: 1516 906 3489 Fragments arranged by size: 3489 1516 906 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4555 4742 1700 Size: 110 2745 187 2869 Fragments arranged by size: 2869 2745 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 2686 2932 96 Size: 1911 679 246 3075 Fragments arranged by size: 3075 1911 679 246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5911 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2970 2970 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2257 2277 2593 2923 3241 3608 3993 4010 Size: 285 20 316 330 318 367 385 17 Cuts at: 4010 5462 5548 5560 5906 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 330 318 316 285 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5359 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 3427 4221 4241 3427 Size: 794 20 5097 Fragments arranged by size: 5097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 2884 3215 2884 Size: 331 5580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3358 4247 Size: 54 1425 50 275 18 919 609 889 Cuts at: 4247 5876 8 Size: 1629 43 Fragments arranged by size: 1629 1425 919 889 609 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2315 2949 3841 1353 Size: 660 302 634 892 3423 Fragments arranged by size: 3423 892 660 634 302 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2479 3974 4571 5358 5626 1239 Size: 393 366 481 1495 597 787 268 1524 Fragments arranged by size: 1524 1495 787 597 481 393 366 268 EcoO109I rG'GnC_Cy Cuts at: 2631 2631 Size: 5911 EcoRI G'AATT_C Cuts at: 2943 3220 3598 2943 Size: 277 378 5256 Fragments arranged by size: 5256 378 277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 5911 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2345 5058 1678 Size: 667 2713 2531 Fragments arranged by size: 2713 2531 667 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2410 2442 4552 Size: 32 135 32 535 32 271 32 2110 Cuts at: 4552 4584 4626 4658 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2110 535 271 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 2574 2905 3079 748 Size: 1826 331 174 3580 Fragments arranged by size: 3580 1826 331 174 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5025 5209 1305 Size: 3720 184 2007 Fragments arranged by size: 3720 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2751 10 Size: 54 1425 343 919 3170 Fragments arranged by size: 3170 1425 919 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2100 2470 2538 2554 2790 2968 3126 Size: 648 934 370 68 16 236 178 158 Cuts at: 3126 3869 4228 4387 518 Size: 743 359 159 2042 Fragments arranged by size: 2042 934 743 648 370 359 236 178 159 158 68 16 MspA1I CmG'CkG Cuts at: 1606 3327 4004 5152 5397 1606 Size: 1721 677 1148 245 2120 Fragments arranged by size: 2120 1721 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 2097 387 Size: 1710 4201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NsiI A_TGCA'T Cuts at: 2103 2103 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 2369 2391 3269 4814 1132 Size: 97 1140 22 878 1545 2229 Fragments arranged by size: 2229 1545 1140 878 97 22 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 2387 4810 2387 Size: 2423 3488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 2217 2301 3481 2217 Size: 84 1180 4647 Fragments arranged by size: 4647 1180 84 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SapI GCTCTTCn'nnn_ Cuts at: 2315 2315 Size: 5911 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2914 3259 4394 5075 5266 826 Size: 219 1869 345 1135 681 191 1471 Fragments arranged by size: 1869 1471 1135 681 345 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 3167 4015 4916 5178 820 Size: 197 926 618 606 848 901 262 1553 Fragments arranged by size: 1553 926 901 848 618 606 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5911 StyI C'CwwG_G Cuts at: 513 1065 2998 3350 513 Size: 552 1933 352 3074 Fragments arranged by size: 3074 1933 552 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3938 3955 4108 4293 4738 325 Size: 1255 2358 17 153 185 445 1498 Fragments arranged by size: 2358 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2504 2686 Size: 274 80 33 51 1443 30 497 182 Cuts at: 2686 2932 3118 4157 5869 96 Size: 246 186 1039 1712 138 Fragments arranged by size: 1712 1443 1039 497 274 246 186 182 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 2561 1943 Size: 618 5293 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI