(Circular) (Six-base) MAPSORT of: pFN21AB9512.seq Check: 8724 from: 1 to: 6214 pFN21AB9512 6214 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2098 2516 2825 2999 2098 Size: 418 309 174 5313 Fragments arranged by size: 5313 418 309 174 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 2924 3535 2924 Size: 611 5603 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3062 4277 5661 5929 1632 Size: 1430 1215 1384 268 1917 Fragments arranged by size: 1917 1430 1384 1215 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 2877 5113 6090 1203 Size: 1674 2236 977 1327 Fragments arranged by size: 2236 1674 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2420 3957 5529 2420 Size: 1537 1572 3105 Fragments arranged by size: 3105 1572 1537 ApaI G_GGCC'C Cuts at: 2199 2468 2199 Size: 269 5945 ApaLI G'TGCA_C Cuts at: 3139 4271 5427 3139 Size: 1132 1156 3926 Fragments arranged by size: 3926 1156 1132 ApoI r'AATT_y Cuts at: 1493 3523 3749 3901 6010 1493 Size: 2030 226 152 2109 1697 Fragments arranged by size: 2109 2030 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2237 4768 160 Size: 872 1205 2531 1606 Fragments arranged by size: 2531 1606 1205 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2229 2268 3239 3505 3539 1870 Size: 73 93 193 39 971 266 34 4545 Fragments arranged by size: 4545 971 266 193 93 73 39 34 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2924 3535 4990 618 Size: 325 217 627 567 570 611 1455 1842 Fragments arranged by size: 1842 1455 627 611 570 567 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2199 2390 2468 2574 3074 3212 3240 Size: 622 186 191 78 106 500 138 28 Cuts at: 3240 3342 3533 1391 Size: 102 191 4072 Fragments arranged by size: 4072 622 500 191 191 186 138 106 102 78 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5243 BbvCI CC'TCA_GC Cuts at: 2487 2487 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3230 3264 4405 4439 1903 Size: 34 1293 34 1141 34 3678 Fragments arranged by size: 3678 1293 1141 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 4825 136 Size: 107 122 71 631 605 349 2804 1525 Fragments arranged by size: 2804 1525 631 605 349 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2199 2468 3143 4275 5431 1790 Size: 409 269 675 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 675 409 269 BmgBI CAC'GTC Cuts at: 1318 2698 1318 Size: 1380 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3945 4903 442 Size: 416 53 83 399 24 2528 958 1753 Fragments arranged by size: 2528 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 2430 4874 1239 Size: 927 264 2444 2579 Fragments arranged by size: 2579 2444 927 264 Bpu10I CC'TnA_GC Cuts at: 2487 3468 2487 Size: 981 5233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2658 4339 5204 5502 2658 Size: 1681 865 298 3370 Fragments arranged by size: 3370 1681 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 3052 4877 882 Size: 617 1239 314 1825 2219 Fragments arranged by size: 2219 1825 1239 617 314 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2376 3438 4647 5045 5319 5466 5877 2376 Size: 1062 1209 398 274 147 411 2713 Fragments arranged by size: 2713 1209 1062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 2598 2628 1178 Size: 30 165 30 987 30 178 30 4764 Fragments arranged by size: 4764 987 178 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2114 2229 1186 Size: 925 3 115 5171 Fragments arranged by size: 5171 925 115 3 BseYI C'CCAG_C Cuts at: 2341 5417 5913 2341 Size: 3076 496 2642 Fragments arranged by size: 3076 2642 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2152 1163 Size: 573 411 5 5225 Fragments arranged by size: 5225 573 411 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 3664 4425 5453 1540 Size: 275 186 31 1632 761 1028 2301 Fragments arranged by size: 2301 1632 1028 761 275 186 31 BsiHKAI G_wGCw'C Cuts at: 2574 3143 4275 4360 5431 2574 Size: 569 1132 85 1071 3357 Fragments arranged by size: 3357 1132 1071 569 85 BsmI GAATG_Cn' Cuts at: 3253 3366 3725 3818 3253 Size: 113 359 93 5649 Fragments arranged by size: 5649 359 113 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2199 2390 2468 2574 3074 3143 Size: 399 223 186 191 78 106 500 69 Cuts at: 3143 3212 3240 3342 3533 4275 4360 5431 1391 Size: 69 28 102 191 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 223 191 191 186 106 102 85 78 69 69 28 BspEI T'CCGG_A Cuts at: 2376 5877 2376 Size: 3501 2713 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 2516 2825 2999 3309 3569 844 Size: 1254 418 309 174 310 260 3489 Fragments arranged by size: 3489 1254 418 310 309 260 174 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4709 4883 65 Size: 1183 3461 174 1396 Fragments arranged by size: 3461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2351 3454 4858 5045 1700 Size: 110 205 336 1103 1404 187 2869 Fragments arranged by size: 2869 1404 1103 336 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6214 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstYI r'GATC_y Cuts at: 1972 3098 3544 3911 4296 4313 5765 5851 5863 Size: 1126 446 367 385 17 1452 86 12 Cuts at: 5863 6209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1126 446 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 2518 513 Size: 552 939 450 64 4209 Fragments arranged by size: 4209 939 552 450 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2164 3730 4524 4544 2164 Size: 1566 794 20 3834 Fragments arranged by size: 3834 1566 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2748 2783 529 Size: 35 2184 35 3960 Fragments arranged by size: 3960 2184 35 35 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 2497 2497 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3044 5221 809 Size: 507 1728 2177 1802 Fragments arranged by size: 2177 1802 1728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2154 3661 Size: 54 1425 50 275 18 199 125 1507 Cuts at: 3661 4550 6179 8 Size: 889 1629 43 Fragments arranged by size: 1629 1507 1425 889 275 199 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2029 3661 1537 Size: 275 217 1632 4090 Fragments arranged by size: 4090 1632 275 217 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 4144 1353 Size: 1371 1420 3423 Fragments arranged by size: 3423 1420 1371 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2572 2572 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2430 3062 4277 4874 5661 5929 Size: 393 534 264 632 1215 597 787 268 Cuts at: 5929 1239 Size: 1524 Fragments arranged by size: 1524 1215 787 632 597 534 393 268 264 EcoNI CCTnn'n_nnAGG Cuts at: 3305 3305 Size: 6214 EcoO109I rG'GnC_Cy Cuts at: 2195 2465 2713 3502 2195 Size: 270 248 789 4907 Fragments arranged by size: 4907 789 270 248 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 EcoRV GAT'ATC Cuts at: 3280 3280 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 2600 2632 2654 Size: 32 135 32 818 32 178 32 22 Cuts at: 2654 2686 4855 4887 4929 4961 1373 Size: 32 2169 32 42 32 2626 Fragments arranged by size: 2626 2169 818 178 135 42 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3804 669 Size: 988 281 1620 246 3079 Fragments arranged by size: 3079 1620 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 2928 3539 2928 Size: 611 5603 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 3372 5328 5512 1305 Size: 1539 528 1956 184 2007 Fragments arranged by size: 2007 1956 1539 528 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4392 Fragments arranged by size: 4392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2973 4172 4531 4690 518 Size: 648 1807 1199 359 159 2042 Fragments arranged by size: 2042 1807 1199 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2144 2854 3020 3086 3630 4307 5455 Size: 438 100 710 166 66 544 677 1148 Cuts at: 5455 5700 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 710 677 544 438 245 166 100 66 NaeI GCC'GGC Cuts at: 2017 2353 3456 2017 Size: 336 1103 4775 Fragments arranged by size: 4775 1103 336 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 3286 387 Size: 2899 3315 NgoMIV G'CCGG_C Cuts at: 2015 2351 3454 2015 Size: 336 1103 4775 Fragments arranged by size: 4775 1103 336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3205 1348 Size: 1857 4357 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2205 1108 Size: 1097 5117 PfoI T'CCnGG_A Cuts at: 2882 2882 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2713 3502 2713 Size: 789 5425 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 2195 2464 2195 Size: 269 5945 PstI C_TGCA'G Cuts at: 830 2109 3566 830 Size: 1279 1457 3478 Fragments arranged by size: 3478 1457 1279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2144 2854 2144 Size: 710 5504 SacI G_AGCT'C Cuts at: 2574 2574 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SanDI GG'GwC_CC Cuts at: 2713 2713 Size: 6214 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SexAI A'CCwGG_T Cuts at: 2802 2858 2802 Size: 56 6158 SfcI C'TryA_G Cuts at: 826 1045 2105 2620 2849 3562 4697 5378 5569 Size: 219 1060 515 229 713 1135 681 191 Cuts at: 5569 826 Size: 1471 Fragments arranged by size: 1471 1135 1060 713 681 515 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 2231 2270 3541 1872 Size: 359 39 1271 4545 Fragments arranged by size: 4545 1271 359 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 4318 5219 5481 820 Size: 197 926 730 1645 901 262 1553 Fragments arranged by size: 1645 1553 926 901 730 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 2766 3306 3653 513 Size: 552 939 636 126 540 347 3074 Fragments arranged by size: 3074 939 636 552 540 347 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 2776 3119 4241 4258 4411 4596 Size: 1255 965 231 343 1122 17 153 185 Cuts at: 4596 5041 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1122 965 445 343 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4460 6172 96 Size: 274 80 33 51 1443 2483 1712 138 Fragments arranged by size: 2483 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2598 2630 6174 6206 2598 Size: 32 3544 32 2606 Fragments arranged by size: 3544 2606 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3425 1316 Size: 2109 4105 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 2229 2268 3539 1870 Size: 359 39 1271 4545 Fragments arranged by size: 4545 1271 359 39 XmnI GAAnn'nnTTC Cuts at: 2552 4343 2552 Size: 1791 4423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI