(Circular) (Six-base) MAPSORT of: pFN21AB9509.seq Check: 9137 from: 1 to: 5785 pFN21AB9509 5785 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2168 2315 2657 2975 3848 5232 5500 Size: 465 71 147 342 318 873 1384 268 Cuts at: 5500 1632 Size: 1917 Fragments arranged by size: 1917 1384 873 465 342 318 268 147 71 AflII C'TTAA_G Cuts at: 820 1017 2548 820 Size: 197 1531 4057 Fragments arranged by size: 4057 1531 197 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 2112 1166 Size: 946 4839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3081 1184 Size: 1897 3888 AlwNI CAG_nnn'CTG Cuts at: 2146 2680 2755 3528 5100 2146 Size: 534 75 773 1572 2831 Fragments arranged by size: 2831 1572 773 534 75 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2326 3094 3320 3472 5581 1493 Size: 833 768 226 152 2109 1697 Fragments arranged by size: 2109 1697 833 768 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3106 4561 618 Size: 325 217 627 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 627 325 217 BanII G_rGCy'C Cuts at: 1391 3104 1391 Size: 1713 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2312 2360 928 Size: 971 413 48 4353 Fragments arranged by size: 4353 971 413 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2101 2135 3976 4010 1903 Size: 34 164 34 1841 34 3678 Fragments arranged by size: 3678 1841 164 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BfrBI ATG'CAT Cuts at: 2340 2340 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2790 4396 Size: 107 122 71 631 605 337 781 1606 Cuts at: 4396 136 Size: 1525 Fragments arranged by size: 1606 1525 781 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 3846 5002 1790 Size: 2056 1156 2573 Fragments arranged by size: 2573 2056 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3010 3516 4474 Size: 416 53 83 399 24 1593 506 958 Cuts at: 4474 442 Size: 1753 Fragments arranged by size: 1753 1593 958 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2438 2456 2654 2834 2956 2993 3008 Size: 1133 66 18 198 180 122 37 15 Cuts at: 3008 4445 1239 Size: 1437 2579 Fragments arranged by size: 2579 1437 1133 198 180 122 66 37 18 15 Bpu10I CC'TnA_GC Cuts at: 2780 2780 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2948 3910 4775 5073 2948 Size: 962 865 298 3660 Fragments arranged by size: 3660 962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 2253 5779 2253 Size: 3526 2259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2627 2657 2966 2996 1178 Size: 30 165 30 1224 30 309 30 3967 Fragments arranged by size: 3967 1224 309 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2186 2369 1186 Size: 988 12 183 4602 Fragments arranged by size: 4602 988 183 12 BseYI C'CCAG_C Cuts at: 2581 4988 5484 2581 Size: 2407 496 2882 Fragments arranged by size: 2882 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3104 3846 3931 5002 1391 Size: 399 1314 742 85 1071 2174 Fragments arranged by size: 2174 1314 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2772 3140 844 Size: 1928 368 3489 Fragments arranged by size: 3489 1928 368 BsrBI CCG'CTC Cuts at: 1394 1823 2164 2992 3672 1394 Size: 429 341 828 680 3507 Fragments arranged by size: 3507 828 680 429 341 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 4280 4454 65 Size: 1183 1596 1436 174 1396 Fragments arranged by size: 1596 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2724 3021 4429 4616 1700 Size: 110 914 297 1408 187 2869 Fragments arranged by size: 2869 1408 914 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2971 2224 Size: 747 5038 BstYI r'GATC_y Cuts at: 1972 2380 2585 3115 3482 3867 3884 5336 5422 Size: 408 205 530 367 385 17 1452 86 Cuts at: 5422 5434 5780 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 530 408 385 367 346 205 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2103 487 Size: 683 56 877 4169 Fragments arranged by size: 4169 877 683 56 BtsI GCAGTG_nn' Cuts at: 2119 3301 4095 4115 2119 Size: 1182 794 20 3789 Fragments arranged by size: 3789 1182 794 20 ClaI AT'CG_AT Cuts at: 1554 3063 1554 Size: 1509 4276 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2066 4792 809 Size: 507 750 2726 1802 Fragments arranged by size: 2726 1802 750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 3232 4121 Size: 54 1425 50 275 18 294 1108 889 Cuts at: 4121 5750 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1108 889 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2516 3715 1353 Size: 1163 1199 3423 Fragments arranged by size: 3423 1199 1163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2309 3040 4372 4746 4892 Size: 1064 138 438 282 731 1332 374 146 Cuts at: 4892 387 Size: 1280 Fragments arranged by size: 1332 1280 1064 731 438 374 282 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2168 2315 2372 2438 2456 2654 Size: 393 465 71 147 57 66 18 198 Cuts at: 2654 2657 2834 2956 2975 2993 3008 3848 4445 Size: 3 177 122 19 18 15 840 597 Cuts at: 4445 5232 5500 1239 Size: 787 268 1524 Fragments arranged by size: 1524 840 787 597 465 393 268 198 177 147 122 71 66 57 19 18 18 15 3 EcoO109I rG'GnC_Cy Cuts at: 2176 2809 3010 2176 Size: 633 201 4951 Fragments arranged by size: 4951 633 201 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2426 4932 1678 Size: 748 2506 2531 Fragments arranged by size: 2531 2506 748 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2570 2602 2728 Size: 32 135 32 826 32 140 32 126 Cuts at: 2728 2760 2834 2866 4426 4458 4500 4532 1373 Size: 32 74 32 1560 32 42 32 2626 Fragments arranged by size: 2626 1560 826 140 135 126 74 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2059 2485 2493 2521 3129 3375 Size: 988 281 121 426 8 28 608 246 Cuts at: 3375 669 Size: 3079 Fragments arranged by size: 3079 988 608 426 281 246 121 28 8 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 4899 5083 1305 Size: 1515 2079 184 2007 Fragments arranged by size: 2079 2007 1515 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3963 Fragments arranged by size: 3963 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 3743 4102 4261 518 Size: 648 946 1631 359 159 2042 Fragments arranged by size: 2042 1631 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3201 3878 5026 5271 1606 Size: 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NsiI A_TGCA'T Cuts at: 2342 2342 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PacI TTA_AT'TAA Cuts at: 2334 2334 Size: 5785 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 3010 2176 Size: 834 4951 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 2751 3137 830 Size: 1921 386 3478 Fragments arranged by size: 3478 1921 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2754 2754 Size: 5785 SfcI C'TryA_G Cuts at: 826 1045 2360 2567 2747 3133 4268 4949 5140 Size: 219 1315 207 180 386 1135 681 191 Cuts at: 5140 826 Size: 1471 Fragments arranged by size: 1471 1315 1135 681 386 219 207 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2548 2927 3889 4790 5052 820 Size: 197 926 605 379 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 605 379 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 3224 513 Size: 552 939 1220 3074 Fragments arranged by size: 3074 1220 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4031 5743 96 Size: 274 80 33 51 1443 2054 1712 138 Fragments arranged by size: 2054 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 2443 3121 2443 Size: 678 5107 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI