Restriction map for
pFN21AB9502
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI AspEI AviII
AvrII BbeI BbvCI BcgI BfrI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HpaI KasI KspI
MluI MroI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 68 CGCG CG^CG
Alw44I 1772 GTGCAC G^TGCAC
AlwNI 611 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1772 GTGCAC G^TGCAC
AspI 3424 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 51 CCCGGG C^[CT]CG[GA]G
BamHI 2543 GGATCC G^GATCC
BanII 324 GAGCCC G[GA]GC[CT]^C
BbrPI 3023 CACGTG CAC^GTG
BclI 2208 TGATCA T^GATCA
BglI 18 GCCCGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3023 CACGTG [CT]AC^GT[GA]
BsaI 81 GAGACC GGTCTC(1/5)
BsmI 1539 GCATTC GAATGC(1/-1)
BsoBI 51 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2770 TGTACA T^GTACA
BspDI 503 ATCGAT AT^CGAT
BsrGI 2770 TGTACA T^GTACA
BstEII 2791 GGTTACC G^GT[ACGT]ACC
BstPI 2791 GGTTACC G^GT[ACGT]ACC
BstUI 68 CGCG CG^CG
ClaI 503 ATCGAT AT^CGAT
DraII 1626 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 2539 CCATGG C^C[GA][CT]GG
EcoNI 1729 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1626 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2791 GGTTACC G^GT[ACGT]ACC
EcoRI 792 GAATTC G^AATTC
FbaI 2208 TGATCA T^GATCA
HaeII 8 GGCGCT [GA]GCGC^[CT]
HgaI 1089 GCGTC GACGC(5/10)
MfeI 2192 CAATTG C^AATTG
MunI 2192 CAATTG C^AATTG
MvnI 68 CGCG CG^CG
NcoI 2539 CCATGG C^CATGG
PflFI 3424 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1438 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3023 CACGTG CAC^GTG
PmlI 3023 CACGTG CAC^GTG
PpuMI 1626 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 1146 ACCTGGT A^CC[AT]GGT
SmaI 51 CCCGGG CCC^GGG
SphI 14 GCATGC GCATG^C
SspBI 2770 TGTACA T^GTACA
Tth111I 3424 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1438 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1288 GGTACC G^GTACC
3507 GGTACC
AflIII 191 ACATGT A^C[GA][CT]GT
1974 ACATGT
Asp718I 1288 GGTACC G^GTACC
3507 GGTACC
BalI 1245 TGGCCA TGG^CCA
2536 TGGCCA
BciVI 3437 GGATAC GTATCC(6/5)
1694 GTATCC
BsaBI 559 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2209 GATCATCATC
BsgI 2470 GTGCAG GTGCAG(16/14)
3145 GTGCAG
BspLU11I 191 ACATGT A^CATGT
1974 ACATGT
Bst1107I 380 GTATAC GTA^TAC
2268 GTATAC
BstZ17I 380 GTATAC GTA^TAC
2268 GTATAC
DraI 2742 TTTAAA TTT^AAA
2833 TTTAAA
DraIII 1145 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3334 CACTTTGTG
EaeI 1245 TGGCCA [CT]^GGCC[GA]
2536 TGGCCA
HindIII 338 AAGCTT A^AGCTT
3070 AAGCTT
KpnI 1288 GGTACC GGTAC^C
3507 GGTACC
MamI 559 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2209 GATCATCATC
MluNI 1245 TGGCCA TGG^CCA
2536 TGGCCA
MscI 1245 TGGCCA TGG^CCA
2536 TGGCCA
NheI 1357 GCTAGC G^CTAGC
1384 GCTAGC
SmlI 930 CTCAAG C^T[CT][GA]AG
1081 CTTGAG
SspI 422 AATATT AAT^ATT
2229 AATATT
TaqI 504 TCGA T^CGA
567 TCGA
TthHB8I 504 TCGA T^CGA
567 TCGA
XcmI 366 CCATGAAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2530 CCAGTTTGGCCATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI