(Circular) (Six-base) MAPSORT of: pFN21AB9502.seq Check: 5948 from: 1 to: 8224 pFN21AB9502 8224 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6034 278 Size: 53 83 186 936 4498 2468 Fragments arranged by size: 4498 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2386 4274 4881 5567 1937 Size: 449 1888 607 686 4594 Fragments arranged by size: 4594 1888 686 607 449 Acc65I G'GTAC_C Cuts at: 3293 5512 5545 3293 Size: 2219 33 5972 Fragments arranged by size: 5972 2219 33 AclI AA'CG_TT Cuts at: 1563 6351 6724 1563 Size: 4788 373 3063 Fragments arranged by size: 4788 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2958 3164 3356 3597 3705 3720 4507 4613 Size: 1326 206 192 241 108 15 787 106 Cuts at: 4613 6287 7671 7939 1632 Size: 1674 1384 268 1917 Fragments arranged by size: 1917 1674 1384 1326 787 268 241 206 192 108 106 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8027 AflIII A'CryG_T Cuts at: 1203 2196 3979 7123 8100 1203 Size: 993 1783 3144 977 1327 Fragments arranged by size: 3144 1783 1327 993 977 AgeI A'CCGG_T Cuts at: 7055 7055 Size: 8224 AhdI GACnn_n'nnGTC Cuts at: 6953 6953 Size: 8224 AleI CACnn'nnGTG Cuts at: 1166 4937 1166 Size: 3771 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8224 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 2116 Size: 32 8192 AlwNI CAG_nnn'CTG Cuts at: 2621 5522 5967 7539 2621 Size: 2901 445 1572 3306 Fragments arranged by size: 3306 2901 1572 445 ApaLI G'TGCA_C Cuts at: 3777 6281 7437 3777 Size: 2504 1156 4564 Fragments arranged by size: 4564 2504 1156 ApoI r'AATT_y Cuts at: 1493 2708 2797 2996 3122 3235 3621 3757 4172 Size: 1215 89 199 126 113 386 136 415 Cuts at: 4172 4184 4260 5533 5759 5911 8020 1493 Size: 12 76 1273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1273 1215 415 386 226 199 152 136 126 113 89 76 12 AseI AT'TA_AT Cuts at: 160 1032 6778 160 Size: 872 5746 1606 Fragments arranged by size: 5746 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8224 AvaI C'yCGr_G Cuts at: 1870 1943 2056 5549 1870 Size: 73 113 3493 4545 Fragments arranged by size: 4545 3493 113 73 BamHI G'GATC_C Cuts at: 4548 5554 5921 4548 Size: 1006 367 6851 Fragments arranged by size: 6851 1006 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2314 2676 3226 3293 4939 Size: 325 217 627 527 362 550 67 1646 Cuts at: 4939 5512 5545 7000 618 Size: 573 33 1455 1842 Fragments arranged by size: 1842 1646 1455 627 573 550 527 362 325 217 67 33 BanII G_rGCy'C Cuts at: 1391 2333 5543 1391 Size: 942 3210 4072 Fragments arranged by size: 4072 3210 942 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3152 4041 4083 4134 4653 5225 928 Size: 971 1253 889 42 51 519 572 3927 Fragments arranged by size: 3927 1253 971 889 572 519 51 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6415 6449 1903 Size: 34 4478 34 3678 Fragments arranged by size: 4478 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 5436 6116 7326 3710 Size: 1726 680 1210 4608 Fragments arranged by size: 4608 1726 1210 680 BclI T'GATC_A Cuts at: 1548 4213 1548 Size: 2665 5559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2029 6835 136 Size: 107 122 71 631 605 357 4806 1525 Fragments arranged by size: 4806 1525 631 605 357 122 107 71 BglII A'GATC_T Cuts at: 3034 3953 4604 8219 3034 Size: 919 651 3615 3039 Fragments arranged by size: 3615 3039 919 651 BlpI GC'TnA_GC Cuts at: 5641 5641 Size: 8224 Bme1580I G_kGCm'C Cuts at: 1790 3781 6285 7441 1790 Size: 1991 2504 1156 2573 Fragments arranged by size: 2573 2504 1991 1156 BmgBI CAC'GTC Cuts at: 1318 2399 1318 Size: 1081 7143 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2265 2708 5955 Size: 416 53 83 399 24 848 443 3247 Cuts at: 5955 6913 442 Size: 958 1753 Fragments arranged by size: 3247 1753 958 848 443 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3366 3393 1056 Size: 2310 27 5887 Fragments arranged by size: 5887 2310 27 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3538 3538 Size: 8224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2626 4640 4957 6884 1239 Size: 1387 2014 317 1927 2579 Fragments arranged by size: 2579 2014 1927 1387 317 Bpu10I CC'TnA_GC Cuts at: 2651 2651 Size: 8224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2920 3107 6349 7214 7512 2920 Size: 187 3242 865 298 3632 Fragments arranged by size: 3632 3242 865 298 187 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 6887 882 Size: 617 581 4807 2219 Fragments arranged by size: 4807 2219 617 581 BsaAI yAC'GTr Cuts at: 493 1204 5030 6015 493 Size: 711 3826 985 2702 Fragments arranged by size: 3826 2702 985 711 BsaBI GATnn'nnATC Cuts at: 2568 4218 8218 2568 Size: 1650 4000 2574 Fragments arranged by size: 4000 2574 1650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6031 6413 7048 275 Size: 53 83 186 936 4498 382 635 1451 Fragments arranged by size: 4498 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6657 7055 7329 7476 7887 6657 Size: 398 274 147 411 6994 Fragments arranged by size: 6994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2474 2504 3652 3682 1178 Size: 30 165 30 1071 30 1148 30 5720 Fragments arranged by size: 5720 1148 1071 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8224 BseYI C'CCAG_C Cuts at: 2050 7427 7923 2050 Size: 5377 496 2351 Fragments arranged by size: 5377 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4496 5171 1163 Size: 573 2760 675 4216 Fragments arranged by size: 4216 2760 675 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5674 6435 7463 1540 Size: 275 186 3673 761 1028 2301 Fragments arranged by size: 3673 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 3490 3781 4167 6285 6370 7441 2370 Size: 1120 291 386 2118 85 1071 3153 Fragments arranged by size: 3153 2118 1120 1071 386 291 85 BsmI GAATG_Cn' Cuts at: 3544 5735 5828 3544 Size: 2191 93 5940 Fragments arranged by size: 5940 2191 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2370 3490 3781 4167 5543 6285 Size: 399 543 37 1120 291 386 1376 742 Cuts at: 6285 6370 7441 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1376 1120 1071 742 543 399 386 291 85 37 BspEI T'CCGG_A Cuts at: 7887 7887 Size: 8224 BspHI T'CATG_A Cuts at: 2349 2542 5500 6113 7843 2349 Size: 193 2958 613 1730 2730 Fragments arranged by size: 2958 2730 1730 613 193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 3758 5113 5579 844 Size: 2437 477 1355 466 3489 Fragments arranged by size: 3489 2437 1355 477 466 BsrBI CCG'CTC Cuts at: 1394 1823 6111 1394 Size: 429 4288 3507 Fragments arranged by size: 4288 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6719 6893 65 Size: 1183 5471 174 1396 Fragments arranged by size: 5471 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6868 7055 1700 Size: 110 5058 187 2869 Fragments arranged by size: 5058 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4775 96 Size: 4679 3545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8224 BssSI C'ACGA_G Cuts at: 6284 7296 6284 Size: 1012 7212 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8224 BstBI TT'CG_AA Cuts at: 7028 7028 Size: 8224 BstEII G'GTnAC_C Cuts at: 1184 4796 1184 Size: 3612 4612 BstYI r'GATC_y Cuts at: 1972 2945 3034 3953 4548 4604 5554 5921 6306 Size: 973 89 919 595 56 950 367 385 Cuts at: 6306 6323 7775 7861 7873 8219 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 973 950 919 595 385 367 346 89 86 56 17 12 BstZ17I GTA'TAC Cuts at: 2387 4275 2387 Size: 1888 6336 BtgI C'CryG_G Cuts at: 513 1065 4544 513 Size: 552 3479 4193 Fragments arranged by size: 4193 3479 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5123 487 Size: 683 56 3897 3588 Fragments arranged by size: 3897 3588 683 56 BtsI GCAGTG_nn' Cuts at: 3781 5740 6534 6554 3781 Size: 1959 794 20 5451 Fragments arranged by size: 5451 1959 794 20 ClaI AT'CG_AT Cuts at: 1554 2509 1554 Size: 955 7269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8189 DraI TTT'AAA Cuts at: 4749 4840 5528 4749 Size: 91 688 7445 Fragments arranged by size: 7445 688 91 DraIII CAC_nnn'GTG Cuts at: 3155 5344 3155 Size: 2189 6035 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7231 809 Size: 507 5915 1802 Fragments arranged by size: 5915 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3250 4541 5671 Size: 54 1425 50 275 18 1420 1291 1130 Cuts at: 5671 6560 8189 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1420 1291 1130 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5671 1537 Size: 275 3859 4090 Fragments arranged by size: 4090 3859 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2956 3217 3495 3849 4155 6154 1353 Size: 1603 261 278 354 306 1999 3423 Fragments arranged by size: 3423 1999 1603 354 306 278 261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6811 7185 7331 387 Size: 1064 138 5222 374 146 1280 Fragments arranged by size: 5222 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2626 2958 3164 3356 3597 3705 3720 Size: 393 994 332 206 192 241 108 15 Cuts at: 3720 4507 4613 4640 4957 6287 6884 7671 7939 Size: 787 106 27 317 1330 597 787 268 Cuts at: 7939 1239 Size: 1524 Fragments arranged by size: 1524 1330 994 787 787 597 393 332 317 268 241 206 192 108 106 27 15 EcoNI CCTnn'n_nnAGG Cuts at: 3738 3738 Size: 8224 EcoO109I rG'GnC_Cy Cuts at: 3632 3632 Size: 8224 EcoRI G'AATT_C Cuts at: 2797 5533 5911 2797 Size: 2736 378 5110 Fragments arranged by size: 5110 2736 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2967 4397 2967 Size: 1430 6794 FspI TGC'GCA Cuts at: 6730 6730 Size: 8224 HaeII r_GCGC'y Cuts at: 1678 2017 7371 1678 Size: 339 5354 2531 Fragments arranged by size: 5354 2531 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 2474 2506 4589 Size: 32 135 32 663 32 207 32 2083 Cuts at: 4589 4621 5037 5069 6865 6897 6939 6971 1373 Size: 32 416 32 1796 32 42 32 2626 Fragments arranged by size: 2626 2083 1796 663 416 207 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5568 5814 669 Size: 988 281 3630 246 3079 Fragments arranged by size: 3630 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2343 5075 748 Size: 1595 2732 3897 Fragments arranged by size: 3897 2732 1595 HpaI GTT'AAC Cuts at: 5814 5814 Size: 8224 KpnI G_GTAC'C Cuts at: 3297 5516 5549 3297 Size: 2219 33 5972 Fragments arranged by size: 5972 2219 33 MfeI C'AATT_G Cuts at: 4197 5823 4197 Size: 1626 6598 MluI A'CGCG_T Cuts at: 8100 8100 Size: 8224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 7338 7522 1305 Size: 876 5157 184 2007 Fragments arranged by size: 5157 2007 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3252 4543 10 Size: 54 1425 343 1420 1291 3691 Fragments arranged by size: 3691 1425 1420 1291 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4224 4937 5097 6182 6541 6700 518 Size: 648 3058 713 160 1085 359 159 2042 Fragments arranged by size: 3058 2042 1085 713 648 359 160 159 MspA1I CmG'CkG Cuts at: 1606 2114 4574 4861 5008 5640 6317 7465 7710 Size: 508 2460 287 147 632 677 1148 245 Cuts at: 7710 1606 Size: 2120 Fragments arranged by size: 2460 2120 1148 677 632 508 287 245 147 NcoI C'CATG_G Cuts at: 513 1065 4544 513 Size: 552 3479 4193 Fragments arranged by size: 4193 3479 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8224 NheI G'CTAG_C Cuts at: 1052 3362 3389 1052 Size: 2310 27 5887 Fragments arranged by size: 5887 2310 27 NotI GC'GGCC_GC Cuts at: 5671 5671 Size: 8224 NspI r_CATG'y Cuts at: 1132 1229 2023 2200 3983 5375 5582 7127 1132 Size: 97 794 177 1783 1392 207 1545 2229 Fragments arranged by size: 2229 1783 1545 1392 794 207 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8224 PciI A'CATG_T Cuts at: 2196 3979 7123 2196 Size: 1783 3144 3297 Fragments arranged by size: 3297 3144 1783 PflMI CCAn_nnn'nTGG Cuts at: 1108 3449 1108 Size: 2341 5883 PfoI T'CCnGG_A Cuts at: 3988 3988 Size: 8224 PmeI GTTT'AAAC Cuts at: 5528 5528 Size: 8224 PmlI CAC'GTG Cuts at: 5030 5030 Size: 8224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6293 6325 7806 7838 6293 Size: 32 1481 32 6679 Fragments arranged by size: 6679 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3632 3632 Size: 8224 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8224 PsiI TTA'TAA Cuts at: 3128 4710 5794 3128 Size: 1582 1084 5558 Fragments arranged by size: 5558 1582 1084 PstI C_TGCA'G Cuts at: 830 2113 2738 3220 3715 3916 4062 5124 5576 Size: 1283 625 482 495 201 146 1062 452 Cuts at: 5576 830 Size: 3478 Fragments arranged by size: 3478 1283 1062 625 495 482 452 201 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8224 PvuII CAG'CTG Cuts at: 4574 4861 5008 4574 Size: 287 147 7790 Fragments arranged by size: 7790 287 147 SalI G'TCGA_C Cuts at: 1936 5566 1936 Size: 3630 4594 SapI GCTCTTCn'nnn_ Cuts at: 3217 3495 4155 3217 Size: 278 660 7286 Fragments arranged by size: 7286 660 278 SbfI CC_TGCA'GG Cuts at: 5576 5576 Size: 8224 ScaI AGT'ACT Cuts at: 6472 8184 6472 Size: 1712 6512 SexAI A'CCwGG_T Cuts at: 3151 3151 Size: 8224 SfcI C'TryA_G Cuts at: 826 1045 2109 2734 3216 3549 3711 3912 3918 Size: 219 1064 625 482 333 162 201 6 Cuts at: 3918 4058 4317 5120 5572 6707 7388 7579 826 Size: 140 259 803 452 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 803 681 625 482 452 333 259 219 201 191 162 140 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8224 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8224 SmaI CCC'GGG Cuts at: 1872 2058 5551 1872 Size: 186 3493 4545 Fragments arranged by size: 4545 3493 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 2935 3086 6328 7229 7491 820 Size: 197 926 992 151 3242 901 262 1553 Fragments arranged by size: 3242 1553 992 926 901 262 197 151 SnaBI TAC'GTA Cuts at: 493 6015 493 Size: 5522 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8224 SphI G_CATG'C Cuts at: 1132 2023 5582 1132 Size: 891 3559 3774 Fragments arranged by size: 3774 3559 891 SspI AAT'ATT Cuts at: 5 52 2429 4236 6148 5 Size: 47 2377 1807 1912 2081 Fragments arranged by size: 2377 2081 1912 1807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8224 StyI C'CwwG_G Cuts at: 513 1065 2858 3183 3297 3942 4278 4544 5663 Size: 552 1793 325 114 645 336 266 1119 Cuts at: 5663 513 Size: 3074 Fragments arranged by size: 3074 1793 1119 645 552 336 325 266 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6251 6268 6421 6606 7051 325 Size: 1255 4671 17 153 185 445 1498 Fragments arranged by size: 4671 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 4487 4775 Size: 274 80 33 51 1443 2156 354 288 Cuts at: 4775 6470 8182 96 Size: 1695 1712 138 Fragments arranged by size: 2156 1712 1695 1443 354 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4362 4394 4678 4710 5014 5046 8184 8216 4362 Size: 32 284 32 304 32 3138 32 4370 Fragments arranged by size: 4370 3138 304 284 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5432 1316 Size: 4116 4108 XbaI T'CTAG_A Cuts at: 5560 5560 Size: 8224 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2378 4542 2378 Size: 2164 6060 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8224 XmaI C'CCGG_G Cuts at: 1870 2056 5549 1870 Size: 186 3493 4545 Fragments arranged by size: 4545 3493 186 XmnI GAAnn'nnTTC Cuts at: 2801 2996 5100 6353 2801 Size: 195 2104 1253 4672 Fragments arranged by size: 4672 2104 1253 195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6032 276 Size: 53 83 186 936 4498 2468 Fragments arranged by size: 4498 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI