(Circular) (Six-base) MAPSORT of: pFN21AB9499.seq Check: 388 from: 1 to: 8107 pFN21AB9499 8107 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4117 4117 Size: 8107 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4373 5917 278 Size: 53 83 186 936 2837 1544 2468 Fragments arranged by size: 2837 2468 1544 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 5015 5450 1937 Size: 1875 1203 435 4594 Fragments arranged by size: 4594 1875 1203 435 Acc65I G'GTAC_C Cuts at: 5428 5428 Size: 8107 AclI AA'CG_TT Cuts at: 1563 6234 6607 1563 Size: 4671 373 3063 Fragments arranged by size: 4671 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2550 3347 5161 6170 7554 7822 1632 Size: 590 328 797 1814 1009 1384 268 1917 Fragments arranged by size: 1917 1814 1384 1009 797 590 328 268 AfeI AGC'GCT Cuts at: 5182 5182 Size: 8107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7910 AflIII A'CryG_T Cuts at: 1203 7006 7983 1203 Size: 5803 977 1327 Fragments arranged by size: 5803 1327 977 AgeI A'CCGG_T Cuts at: 6938 6938 Size: 8107 AhdI GACnn_n'nnGTC Cuts at: 6836 6836 Size: 8107 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3386 1184 Size: 2202 5905 AlwNI CAG_nnn'CTG Cuts at: 2135 2528 2839 3610 4234 4397 5234 5353 5850 Size: 393 311 771 624 163 837 119 497 Cuts at: 5850 7422 2135 Size: 1572 2820 Fragments arranged by size: 2820 1572 837 771 624 497 393 311 163 119 ApaI G_GGCC'C Cuts at: 4383 4493 4383 Size: 110 7997 ApaLI G'TGCA_C Cuts at: 6164 7320 6164 Size: 1156 6951 ApoI r'AATT_y Cuts at: 1493 2324 2756 3041 4079 5416 5642 5794 7903 Size: 831 432 285 1038 1337 226 152 2109 Cuts at: 7903 1493 Size: 1697 Fragments arranged by size: 2109 1697 1337 1038 831 432 285 226 152 AseI AT'TA_AT Cuts at: 160 1032 3168 6661 160 Size: 872 2136 3493 1606 Fragments arranged by size: 3493 2136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8107 AvaI C'yCGr_G Cuts at: 1870 1943 2494 3201 3913 4762 5240 5432 1870 Size: 73 551 707 712 849 478 192 4545 Fragments arranged by size: 4545 849 712 707 551 478 192 73 BamHI G'GATC_C Cuts at: 5437 5804 5437 Size: 367 7740 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2855 3744 5329 5428 6883 Size: 325 217 627 1068 889 1585 99 1455 Cuts at: 6883 618 Size: 1842 Fragments arranged by size: 1842 1585 1455 1068 889 627 325 217 99 BanII G_rGCy'C Cuts at: 1391 2016 3927 4383 4493 5123 5426 1391 Size: 625 1911 456 110 630 303 4072 Fragments arranged by size: 4072 1911 630 625 456 303 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3999 4547 5264 928 Size: 971 2100 548 717 3771 Fragments arranged by size: 3771 2100 971 717 548 BbvCI CC'TCA_GC Cuts at: 3129 3129 Size: 8107 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6298 6332 1903 Size: 34 4361 34 3678 Fragments arranged by size: 4361 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5999 7209 5999 Size: 1210 6897 BclI T'GATC_A Cuts at: 1548 1548 Size: 8107 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3685 6718 136 Size: 107 122 71 631 605 2013 3033 1525 Fragments arranged by size: 3033 2013 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2260 8102 2260 Size: 5842 2265 BlpI GC'TnA_GC Cuts at: 3209 4347 5524 3209 Size: 1138 1177 5792 Fragments arranged by size: 5792 1177 1138 Bme1580I G_kGCm'C Cuts at: 1790 3747 4383 4493 5334 6168 7324 1790 Size: 1957 636 110 841 834 1156 2573 Fragments arranged by size: 2573 1957 1156 841 834 636 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4250 5838 6796 Size: 416 53 83 399 24 2833 1588 958 Cuts at: 6796 442 Size: 1753 Fragments arranged by size: 2833 1753 1588 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4194 1056 Size: 3138 4969 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 8107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2265 2870 2894 2958 3107 3323 3617 3632 Size: 1026 605 24 64 149 216 294 15 Cuts at: 3632 3995 4469 4591 4896 5275 6767 1239 Size: 363 474 122 305 379 1492 2579 Fragments arranged by size: 2579 1492 1026 605 474 379 363 305 294 216 149 122 64 24 15 Bpu10I CC'TnA_GC Cuts at: 2832 3111 3129 4652 2832 Size: 279 18 1523 6287 Fragments arranged by size: 6287 1523 279 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3266 3332 6232 7097 7395 3266 Size: 66 2900 865 298 3978 Fragments arranged by size: 3978 2900 865 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5212 6770 882 Size: 617 3713 1558 2219 Fragments arranged by size: 3713 2219 1558 617 BsaAI yAC'GTr Cuts at: 493 1204 5898 493 Size: 711 4694 2702 Fragments arranged by size: 4694 2702 711 BsaBI GATnn'nnATC Cuts at: 8101 8101 Size: 8107 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4370 5914 6296 6931 Size: 53 83 186 936 2837 1544 382 635 Cuts at: 6931 275 Size: 1451 Fragments arranged by size: 2837 1544 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3471 6540 6938 7212 7359 7770 3471 Size: 3069 398 274 147 411 3808 Fragments arranged by size: 3808 3069 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3557 3587 1178 Size: 30 165 30 2154 30 5698 Fragments arranged by size: 5698 2154 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2807 2810 3461 3951 4006 5053 5179 Size: 913 708 3 651 490 55 1047 126 Cuts at: 5179 1186 Size: 4114 Fragments arranged by size: 4114 1047 913 708 651 490 126 55 3 BseYI C'CCAG_C Cuts at: 2090 4067 4630 7310 7806 2090 Size: 1977 563 2680 496 2391 Fragments arranged by size: 2680 2391 1977 563 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4077 4560 1163 Size: 573 2341 483 4710 Fragments arranged by size: 4710 2341 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5557 6318 7346 1540 Size: 275 186 3556 761 1028 2301 Fragments arranged by size: 3556 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3292 3927 5123 5172 6168 6253 7324 3292 Size: 635 1196 49 996 85 1071 4075 Fragments arranged by size: 4075 1196 1071 996 635 85 49 BsmI GAATG_Cn' Cuts at: 3407 5618 5711 3407 Size: 2211 93 5803 Fragments arranged by size: 5803 2211 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4377 4377 Size: 8107 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 3292 3747 3927 4383 4493 5123 Size: 399 226 1276 455 180 456 110 630 Cuts at: 5123 5172 5334 5426 6168 6253 7324 1391 Size: 49 162 92 742 85 1071 2174 Fragments arranged by size: 2174 1276 1071 742 630 456 455 399 226 180 162 110 92 85 49 BspEI T'CCGG_A Cuts at: 7770 7770 Size: 8107 BspHI T'CATG_A Cuts at: 5996 7726 5996 Size: 1730 6377 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4117 5462 844 Size: 3273 1345 3489 Fragments arranged by size: 3489 3273 1345 BsrBI CCG'CTC Cuts at: 1394 1823 3225 5994 1394 Size: 429 1402 2769 3507 Fragments arranged by size: 3507 2769 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3864 6602 6776 65 Size: 1183 2616 2738 174 1396 Fragments arranged by size: 2738 2616 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 2672 4430 5057 6751 6938 1700 Size: 110 312 550 1758 627 1694 187 2869 Fragments arranged by size: 2869 1758 1694 627 550 312 187 110 BsrGI T'GTAC_A Cuts at: 96 4136 96 Size: 4040 4067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8107 BssSI C'ACGA_G Cuts at: 6167 7179 6167 Size: 1012 7095 BstAPI GCAn_nnn'nTGC Cuts at: 794 3428 5353 794 Size: 2634 1925 3548 Fragments arranged by size: 3548 2634 1925 BstBI TT'CG_AA Cuts at: 6911 6911 Size: 8107 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8107 BstXI CCAn_nnnn'nTGG Cuts at: 2926 3439 4042 5261 2926 Size: 513 603 1219 5772 Fragments arranged by size: 5772 1219 603 513 BstYI r'GATC_y Cuts at: 1972 2260 2269 5437 5804 6189 6206 7658 7744 Size: 288 9 3168 367 385 17 1452 86 Cuts at: 7744 7756 8102 1972 Size: 12 346 1977 Fragments arranged by size: 3168 1977 1452 385 367 346 288 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 4588 4588 Size: 8107 BtgI C'CryG_G Cuts at: 513 1065 2925 3739 4364 4827 5254 5396 513 Size: 552 1860 814 625 463 427 142 3224 Fragments arranged by size: 3224 1860 814 625 552 463 427 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3383 487 Size: 683 56 2157 5211 Fragments arranged by size: 5211 2157 683 56 BtsI GCAGTG_nn' Cuts at: 2919 4085 5623 6417 6437 2919 Size: 1166 1538 794 20 4589 Fragments arranged by size: 4589 1538 1166 794 20 ClaI AT'CG_AT Cuts at: 1554 2382 2421 1554 Size: 828 39 7240 Fragments arranged by size: 7240 828 39 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5385 5420 529 Size: 35 4821 35 3216 Fragments arranged by size: 4821 3216 35 35 DraI TTT'AAA Cuts at: 5411 5411 Size: 8107 DraIII CAC_nnn'GTG Cuts at: 4512 4512 Size: 8107 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 7114 809 Size: 507 3659 2139 1802 Fragments arranged by size: 3659 2139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2916 3384 3762 Size: 54 1425 50 275 18 1086 468 378 Cuts at: 3762 4548 5055 5263 5554 6443 8072 8 Size: 786 507 208 291 889 1629 43 Fragments arranged by size: 1629 1425 1086 889 786 507 468 378 291 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5554 1537 Size: 275 3742 4090 Fragments arranged by size: 4090 3742 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3061 3438 4648 4762 6037 1353 Size: 1708 377 1210 114 1275 3423 Fragments arranged by size: 3423 1708 1275 1210 377 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6694 7068 7214 387 Size: 1064 138 5105 374 146 1280 Fragments arranged by size: 5105 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3925 5121 3925 Size: 1196 6911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2265 2550 2870 2894 2958 3107 Size: 393 590 43 285 320 24 64 149 Cuts at: 3107 3323 3347 3617 3632 3995 4469 4591 4896 Size: 216 24 270 15 363 474 122 305 Cuts at: 4896 5161 5275 6170 6767 7554 7822 1239 Size: 265 114 895 597 787 268 1524 Fragments arranged by size: 1524 895 787 597 590 474 393 363 320 305 285 270 268 265 216 149 122 114 64 43 24 24 15 EcoNI CCTnn'n_nnAGG Cuts at: 3916 4313 3916 Size: 397 7710 EcoO109I rG'GnC_Cy Cuts at: 2311 2836 3527 3607 3909 4318 4380 4489 4944 Size: 525 691 80 302 409 62 109 455 Cuts at: 4944 5231 5244 5384 2311 Size: 287 13 140 5034 Fragments arranged by size: 5034 691 525 455 409 302 287 140 109 80 62 13 EcoRI G'AATT_C Cuts at: 3041 4079 5416 5794 3041 Size: 1038 1337 378 5354 Fragments arranged by size: 5354 1337 1038 378 FspI TGC'GCA Cuts at: 6613 6613 Size: 8107 HaeII r_GCGC'y Cuts at: 1678 5184 5312 7254 1678 Size: 3506 128 1942 2531 Fragments arranged by size: 3506 2531 1942 128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2630 2662 3914 3946 3991 Size: 32 135 32 1058 32 1252 32 45 Cuts at: 3991 4023 6748 6780 6822 6854 1373 Size: 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 1252 1058 135 45 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3970 4896 5451 5697 669 Size: 988 281 2032 926 555 246 3079 Fragments arranged by size: 3079 2032 988 926 555 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8107 HpaI GTT'AAC Cuts at: 5697 5697 Size: 8107 KpnI G_GTAC'C Cuts at: 5432 5432 Size: 8107 MfeI C'AATT_G Cuts at: 5706 5706 Size: 8107 MluI A'CGCG_T Cuts at: 7983 7983 Size: 8107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2433 4102 7221 7405 1305 Size: 684 444 1669 3119 184 2007 Fragments arranged by size: 3119 2007 1669 684 444 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3386 3764 5265 10 Size: 54 1425 343 1554 378 1501 2852 Fragments arranged by size: 2852 1554 1501 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 3306 6065 6424 6583 518 Size: 648 1758 382 2759 359 159 2042 Fragments arranged by size: 2759 2042 1758 648 382 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2825 4067 4547 5523 6200 7348 7593 Size: 529 690 1242 480 976 677 1148 245 Cuts at: 7593 1606 Size: 2120 Fragments arranged by size: 2120 1242 1148 976 690 677 529 480 245 NaeI GCC'GGC Cuts at: 2124 2674 4432 5059 2124 Size: 550 1758 627 5172 Fragments arranged by size: 5172 1758 627 550 NcoI C'CATG_G Cuts at: 513 1065 2925 3739 5254 513 Size: 552 1860 814 1515 3366 Fragments arranged by size: 3366 1860 1515 814 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8107 NgoMIV G'CCGG_C Cuts at: 2122 2672 4430 5057 2122 Size: 550 1758 627 5172 Fragments arranged by size: 5172 1758 627 550 NheI G'CTAG_C Cuts at: 1052 4190 1052 Size: 3138 4969 NotI GC'GGCC_GC Cuts at: 5554 5554 Size: 8107 NspI r_CATG'y Cuts at: 1132 1229 5465 7010 1132 Size: 97 4236 1545 2229 Fragments arranged by size: 4236 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4948 1348 Size: 3600 4507 PciI A'CATG_T Cuts at: 7006 7006 Size: 8107 PflMI CCAn_nnn'nTGG Cuts at: 1108 3610 3696 4954 1108 Size: 2502 86 1258 4261 Fragments arranged by size: 4261 2502 1258 86 PfoI T'CCnGG_A Cuts at: 2075 2075 Size: 8107 PmeI GTTT'AAAC Cuts at: 5411 5411 Size: 8107 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6176 6208 7689 7721 6176 Size: 32 1481 32 6562 Fragments arranged by size: 6562 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3527 3607 4944 5244 3527 Size: 80 1337 300 6390 Fragments arranged by size: 6390 1337 300 80 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8107 PsiI TTA'TAA Cuts at: 5141 5677 5141 Size: 536 7571 PspOMI G'GGCC_C Cuts at: 4379 4489 4379 Size: 110 7997 PspXI vC'TCGA_Gb Cuts at: 3913 3913 Size: 8107 PstI C_TGCA'G Cuts at: 830 2131 2508 3266 4114 4203 4533 4690 4996 Size: 1301 377 758 848 89 330 157 306 Cuts at: 4996 5459 830 Size: 463 3478 Fragments arranged by size: 3478 1301 848 758 463 377 330 306 157 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8107 PvuII CAG'CTG Cuts at: 2135 4067 2135 Size: 1932 6175 SacI G_AGCT'C Cuts at: 3927 5123 3927 Size: 1196 6911 SalI G'TCGA_C Cuts at: 1936 5449 1936 Size: 3513 4594 SanDI GG'GwC_CC Cuts at: 4944 4944 Size: 8107 SapI GCTCTTCn'nnn_ Cuts at: 4762 4762 Size: 8107 SbfI CC_TGCA'GG Cuts at: 5459 5459 Size: 8107 ScaI AGT'ACT Cuts at: 6355 8067 6355 Size: 1712 6395 SfcI C'TryA_G Cuts at: 826 1045 2127 2504 3262 4110 4199 4529 4686 Size: 219 1082 377 758 848 89 330 157 Cuts at: 4686 4992 5455 6590 7271 7462 826 Size: 306 463 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 848 758 681 463 377 330 306 219 191 157 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8107 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8107 SmaI CCC'GGG Cuts at: 1872 5242 5434 1872 Size: 3370 192 4545 Fragments arranged by size: 4545 3370 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 3281 3347 3913 6211 7112 7374 Size: 197 926 1338 66 566 2298 901 262 Cuts at: 7374 820 Size: 1553 Fragments arranged by size: 2298 1553 1338 926 901 566 262 197 66 SnaBI TAC'GTA Cuts at: 493 5898 493 Size: 5405 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8107 SphI G_CATG'C Cuts at: 1132 5465 1132 Size: 4333 3774 SspI AAT'ATT Cuts at: 5 52 2720 6031 5 Size: 47 2668 3311 2081 Fragments arranged by size: 3311 2668 2081 47 StuI AGG'CCT Cuts at: 1517 2235 3072 3897 4596 1517 Size: 718 837 825 699 5028 Fragments arranged by size: 5028 837 825 718 699 StyI C'CwwG_G Cuts at: 513 1065 2925 3019 3739 4035 5130 5211 5254 Size: 552 1860 94 720 296 1095 81 43 Cuts at: 5254 5260 5546 513 Size: 6 286 3074 Fragments arranged by size: 3074 1860 1095 720 552 296 286 94 81 43 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2379 3173 5351 6134 6151 6304 6489 Size: 1255 799 794 2178 783 17 153 185 Cuts at: 6489 6934 325 Size: 445 1498 Fragments arranged by size: 2178 1498 1255 799 794 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4136 4330 6353 Size: 274 80 33 51 1443 2159 194 2023 Cuts at: 6353 8065 96 Size: 1712 138 Fragments arranged by size: 2159 2023 1712 1443 274 194 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3557 3589 8067 8099 3557 Size: 32 4478 32 3565 Fragments arranged by size: 4478 3565 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5011 1316 Size: 3695 4412 XbaI T'CTAG_A Cuts at: 5443 5443 Size: 8107 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3167 5258 3167 Size: 2091 6016 XhoI C'TCGA_G Cuts at: 1943 3913 1943 Size: 1970 6137 XmaI C'CCGG_G Cuts at: 1870 5240 5432 1870 Size: 3370 192 4545 Fragments arranged by size: 4545 3370 192 XmnI GAAnn'nnTTC Cuts at: 3013 3152 3539 4451 6236 3013 Size: 139 387 912 1785 4884 Fragments arranged by size: 4884 1785 912 387 139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4371 5915 276 Size: 53 83 186 936 2837 1544 2468 Fragments arranged by size: 2837 2468 1544 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI