Restriction map for
pFN21AB9499
(Linear) (Six-base) MAPSORT of: KIBB9499.seq Check: 7606 from: 1 to: 3402
KIBB9499.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2112 3402
Size: 2112 1290
AatII G_ACGT'C
Cuts at: 0 2368 3402
Size: 2368 1034
AccI GT'mk_AC
Cuts at: 0 1807 3010 3402
Size: 1807 1203 392
Fragments arranged by size:
1807 1203 392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 545 1342 3156 3402
Size: 217 328 797 1814 246
Fragments arranged by size:
1814 797 328 246 217
AfeI AGC'GCT
Cuts at: 0 3177 3402
Size: 3177 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1381 3402
Size: 1381 2021
AlwNI CAG_nnn'CTG
Cuts at: 0 130 523 834 1605 2229 2392 3229 3348
Size: 130 393 311 771 624 163 837 119
Cuts at: 3348 3402
Size: 54
Fragments arranged by size:
837 771 624 393 311 163 130 119
54
ApaI G_GGCC'C
Cuts at: 0 2378 2488 3402
Size: 2378 110 914
Fragments arranged by size:
2378 914 110
ApoI r'AATT_y
Cuts at: 0 319 751 1036 2074 3402
Size: 319 432 285 1038 1328
Fragments arranged by size:
1328 1038 432 319 285
AseI AT'TA_AT
Cuts at: 0 1163 3402
Size: 1163 2239
AvaI C'yCGr_G
Cuts at: 0 489 1196 1908 2757 3235 3402
Size: 489 707 712 849 478 167
Fragments arranged by size:
849 712 707 489 478 167
BanI G'GyrC_C
Cuts at: 0 850 1739 3324 3402
Size: 850 889 1585 78
Fragments arranged by size:
1585 889 850 78
BanII G_rGCy'C
Cuts at: 0 11 1922 2378 2488 3118 3402
Size: 11 1911 456 110 630 284
Fragments arranged by size:
1911 630 456 284 110 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1994 2542 3259 3402
Size: 1994 548 717 143
Fragments arranged by size:
1994 717 548 143
BbvCI CC'TCA_GC
Cuts at: 0 1124 3402
Size: 1124 2278
BglI GCCn_nnn'nGGC
Cuts at: 0 1680 3402
Size: 1680 1722
BglII A'GATC_T
Cuts at: 0 255 3402
Size: 255 3147
BlpI GC'TnA_GC
Cuts at: 0 1204 2342 3402
Size: 1204 1138 1060
Fragments arranged by size:
1204 1138 1060
Bme1580I G_kGCm'C
Cuts at: 0 1742 2378 2488 3329 3402
Size: 1742 636 110 841 73
Fragments arranged by size:
1742 841 636 110 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 2245 3402
Size: 2245 1157
BmtI G_CTAG'C
Cuts at: 0 2189 3402
Size: 2189 1213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 3402
Size: 870 2532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 865 889 953 1102 1318 1612 1627
Size: 260 605 24 64 149 216 294 15
Cuts at: 1627 1990 2464 2586 2891 3270 3402
Size: 363 474 122 305 379 132
Fragments arranged by size:
605 474 379 363 305 294 260 216
149 132 122 64 24 15
Bpu10I CC'TnA_GC
Cuts at: 0 827 1106 1124 2647 3402
Size: 827 279 18 1523 755
Fragments arranged by size:
1523 827 755 279 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1327 3402
Size: 1261 66 2075
Fragments arranged by size:
2075 1261 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 3207 3402
Size: 3207 195
BsaHI Gr'CG_yC
Cuts at: 0 2365 3402
Size: 2365 1037
BsaWI w'CCGG_w
Cuts at: 0 1466 3402
Size: 1466 1936
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1552 1582 3402
Size: 1552 30 1820
Fragments arranged by size:
1820 1552 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 802 805 1456 1946 2001 3048 3174
Size: 94 708 3 651 490 55 1047 126
Cuts at: 3174 3402
Size: 228
Fragments arranged by size:
1047 708 651 490 228 126 94 55
3
BseYI C'CCAG_C
Cuts at: 0 85 2062 2625 3402
Size: 85 1977 563 777
Fragments arranged by size:
1977 777 563 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2555 3402
Size: 2072 483 847
Fragments arranged by size:
2072 847 483
BsiHKAI G_wGCw'C
Cuts at: 0 1287 1922 3118 3167 3402
Size: 1287 635 1196 49 235
Fragments arranged by size:
1287 1196 635 235 49
BsmI GAATG_Cn'
Cuts at: 0 1402 3402
Size: 1402 2000
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2372 3402
Size: 2372 1030
Bsp1286I G_dGCh'C
Cuts at: 0 11 1287 1742 1922 2378 2488 3118 3167
Size: 11 1276 455 180 456 110 630 49
Cuts at: 3167 3329 3402
Size: 162 73
Fragments arranged by size:
1276 630 456 455 180 162 110 73
49 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2112 3402
Size: 2112 1290
BsrBI CCG'CTC
Cuts at: 0 1220 3402
Size: 1220 2182
BsrDI GCAATG_nn'
Cuts at: 0 1859 3402
Size: 1859 1543
BsrFI r'CCGG_y
Cuts at: 0 117 667 2425 3052 3402
Size: 117 550 1758 627 350
Fragments arranged by size:
1758 627 550 350 117
BsrGI T'GTAC_A
Cuts at: 0 2131 3402
Size: 2131 1271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1423 3348 3402
Size: 1423 1925 54
Fragments arranged by size:
1925 1423 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 921 1434 2037 3256 3402
Size: 921 513 603 1219 146
Fragments arranged by size:
1219 921 603 513 146
BstYI r'GATC_y
Cuts at: 0 255 264 3402
Size: 255 9 3138
Fragments arranged by size:
3138 255 9
Bsu36I CC'TnA_GG
Cuts at: 0 2583 3402
Size: 2583 819
BtgI C'CryG_G
Cuts at: 0 920 1734 2359 2822 3249 3391 3402
Size: 920 814 625 463 427 142 11
Fragments arranged by size:
920 814 625 463 427 142 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1378 3402
Size: 1378 2024
BtsI GCAGTG_nn'
Cuts at: 0 914 2080 3402
Size: 914 1166 1322
Fragments arranged by size:
1322 1166 914
ClaI AT'CG_AT
Cuts at: 0 377 416 3402
Size: 377 39 2986
Fragments arranged by size:
2986 377 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3380 3402
Size: 3380 22
DraIII CAC_nnn'GTG
Cuts at: 0 2507 3402
Size: 2507 895
DrdI GACnn_nn'nnGTC
Cuts at: 0 2970 3402
Size: 2970 432
EaeI y'GGCC_r
Cuts at: 0 911 1379 1757 2543 3050 3258 3402
Size: 911 468 378 786 507 208 144
Fragments arranged by size:
911 786 507 468 378 208 144
EarI CTCTTCn'nnn_
Cuts at: 0 1056 1433 2643 2757 3402
Size: 1056 377 1210 114 645
Fragments arranged by size:
1210 1056 645 377 114
EcoICRI GAG'CTC
Cuts at: 0 1920 3116 3402
Size: 1920 1196 286
Fragments arranged by size:
1920 1196 286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 260 545 865 889 953 1102 1318
Size: 217 43 285 320 24 64 149 216
Cuts at: 1318 1342 1612 1627 1990 2464 2586 2891 3156
Size: 24 270 15 363 474 122 305 265
Cuts at: 3156 3270 3402
Size: 114 132
Fragments arranged by size:
474 363 320 305 285 270 265 217
216 149 132 122 114 64 43 24
24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1911 2308 3402
Size: 1911 397 1094
Fragments arranged by size:
1911 1094 397
EcoO109I rG'GnC_Cy
Cuts at: 0 306 831 1522 1602 1904 2313 2375 2484
Size: 306 525 691 80 302 409 62 109
Cuts at: 2484 2939 3226 3239 3379 3402
Size: 455 287 13 140 23
Fragments arranged by size:
691 525 455 409 306 302 287 140
109 80 62 23 13
EcoRI G'AATT_C
Cuts at: 0 1036 2074 3402
Size: 1036 1038 1328
Fragments arranged by size:
1328 1038 1036
HaeII r_GCGC'y
Cuts at: 0 3179 3307 3402
Size: 3179 128 95
Fragments arranged by size:
3179 128 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 625 657 1909 1941 1986 2018 3402
Size: 625 32 1252 32 45 32 1384
Fragments arranged by size:
1384 1252 625 45 32 32 32
HincII GTy'rAC
Cuts at: 0 1965 2891 3402
Size: 1965 926 511
Fragments arranged by size:
1965 926 511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 2097 3402
Size: 428 1669 1305
Fragments arranged by size:
1669 1305 428
MscI TGG'CCA
Cuts at: 0 1381 1759 3260 3402
Size: 1381 378 1501 142
Fragments arranged by size:
1501 1381 378 142
MslI CAynn'nnrTG
Cuts at: 0 919 1301 3402
Size: 919 382 2101
Fragments arranged by size:
2101 919 382
MspA1I CmG'CkG
Cuts at: 0 130 820 2062 2542 3402
Size: 130 690 1242 480 860
Fragments arranged by size:
1242 860 690 480 130
NaeI GCC'GGC
Cuts at: 0 119 669 2427 3054 3402
Size: 119 550 1758 627 348
Fragments arranged by size:
1758 627 550 348 119
NcoI C'CATG_G
Cuts at: 0 920 1734 3249 3402
Size: 920 814 1515 153
Fragments arranged by size:
1515 920 814 153
NgoMIV G'CCGG_C
Cuts at: 0 117 667 2425 3052 3402
Size: 117 550 1758 627 350
Fragments arranged by size:
1758 627 550 350 117
NheI G'CTAG_C
Cuts at: 0 2185 3402
Size: 2185 1217
PasI CC'CwG_GG
Cuts at: 0 2943 3402
Size: 2943 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 1605 1691 2949 3402
Size: 1605 86 1258 453
Fragments arranged by size:
1605 1258 453 86
PfoI T'CCnGG_A
Cuts at: 0 70 3402
Size: 70 3332
PpuMI rG'GwC_Cy
Cuts at: 0 1522 1602 2939 3239 3402
Size: 1522 80 1337 300 163
Fragments arranged by size:
1522 1337 300 163 80
PsiI TTA'TAA
Cuts at: 0 3136 3402
Size: 3136 266
PspOMI G'GGCC_C
Cuts at: 0 2374 2484 3402
Size: 2374 110 918
Fragments arranged by size:
2374 918 110
PspXI vC'TCGA_Gb
Cuts at: 0 1908 3402
Size: 1908 1494
PstI C_TGCA'G
Cuts at: 0 126 503 1261 2109 2198 2528 2685 2991
Size: 126 377 758 848 89 330 157 306
Cuts at: 2991 3402
Size: 411
Fragments arranged by size:
848 758 411 377 330 306 157 126
89
PvuII CAG'CTG
Cuts at: 0 130 2062 3402
Size: 130 1932 1340
Fragments arranged by size:
1932 1340 130
SacI G_AGCT'C
Cuts at: 0 1922 3118 3402
Size: 1922 1196 284
Fragments arranged by size:
1922 1196 284
SanDI GG'GwC_CC
Cuts at: 0 2939 3402
Size: 2939 463
SapI GCTCTTCn'nnn_
Cuts at: 0 2757 3402
Size: 2757 645
SfcI C'TryA_G
Cuts at: 0 122 499 1257 2105 2194 2524 2681 2987
Size: 122 377 758 848 89 330 157 306
Cuts at: 2987 3402
Size: 415
Fragments arranged by size:
848 758 415 377 330 306 157 122
89
SmaI CCC'GGG
Cuts at: 0 3237 3402
Size: 3237 165
SmlI C'TyrA_G
Cuts at: 0 1276 1342 1908 3402
Size: 1276 66 566 1494
Fragments arranged by size:
1494 1276 566 66
SspI AAT'ATT
Cuts at: 0 715 3402
Size: 715 2687
StuI AGG'CCT
Cuts at: 0 230 1067 1892 2591 3402
Size: 230 837 825 699 811
Fragments arranged by size:
837 825 811 699 230
StyI C'CwwG_G
Cuts at: 0 920 1014 1734 2030 3125 3206 3249 3255
Size: 920 94 720 296 1095 81 43 6
Cuts at: 3255 3402
Size: 147
Fragments arranged by size:
1095 920 720 296 147 94 81 43
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 374 1168 3346 3402
Size: 374 794 2178 56
Fragments arranged by size:
2178 794 374 56
TatI w'GTAC_w
Cuts at: 0 2131 2325 3402
Size: 2131 194 1077
Fragments arranged by size:
2131 1077 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1552 1584 3402
Size: 1552 32 1818
Fragments arranged by size:
1818 1552 32
Tth111I GACn'n_nGTC
Cuts at: 0 3006 3402
Size: 3006 396
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1162 3253 3402
Size: 1162 2091 149
Fragments arranged by size:
2091 1162 149
XhoI C'TCGA_G
Cuts at: 0 1908 3402
Size: 1908 1494
XmaI C'CCGG_G
Cuts at: 0 3235 3402
Size: 3235 167
XmnI GAAnn'nnTTC
Cuts at: 0 1008 1147 1534 2446 3402
Size: 1008 139 387 912 956
Fragments arranged by size:
1008 956 912 387 139
ZraI GAC'GTC
Cuts at: 0 2366 3402
Size: 2366 1036
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BbvCI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NheI PasI PflMI
PfoI PpuMI PsiI PspOMI PspXI PstI PvuII SacI
SanDI SapI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BmgBI BsaAI BsaBI BsiEI BsiWI
BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I DraI
EagI EciI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI XbaI