Restriction map for
pFN21AB9497
(Linear) (Six-base) MAPSORT of: KIBB9497.seq Check: 737 from: 1 to: 3525
KIBB9497.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1404 1663 3525
Size: 1404 259 1862
Fragments arranged by size:
1862 1404 259
AatII G_ACGT'C
Cuts at: 0 575 3525
Size: 575 2950
AccI GT'mk_AC
Cuts at: 0 1723 3525
Size: 1723 1802
Acc65I G'GTAC_C
Cuts at: 0 408 3525
Size: 408 3117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 1063 1597 2188 2575 2662 3525
Size: 705 358 534 591 387 87 863
Fragments arranged by size:
863 705 591 534 387 358 87
AfeI AGC'GCT
Cuts at: 0 499 2936 3457 3525
Size: 499 2437 521 68
Fragments arranged by size:
2437 521 499 68
AgeI A'CCGG_T
Cuts at: 0 40 654 3525
Size: 40 614 2871
Fragments arranged by size:
2871 614 40
AhdI GACnn_n'nnGTC
Cuts at: 0 1153 3525
Size: 1153 2372
AleI CACnn'nnGTG
Cuts at: 0 1687 2380 2902 3217 3525
Size: 1687 693 522 315 308
Fragments arranged by size:
1687 693 522 315 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1989 2083 3016 3525
Size: 1054 935 94 933 509
Fragments arranged by size:
1054 935 933 509 94
AlwNI CAG_nnn'CTG
Cuts at: 0 678 2013 2032 2103 2199 2874 3525
Size: 678 1335 19 71 96 675 651
Fragments arranged by size:
1335 678 675 651 96 71 19
ApaI G_GGCC'C
Cuts at: 0 1073 1634 2468 2640 3525
Size: 1073 561 834 172 885
Fragments arranged by size:
1073 885 834 561 172
ApaLI G'TGCA_C
Cuts at: 0 1422 3525
Size: 1422 2103
AvaI C'yCGr_G
Cuts at: 0 1133 1930 1981 2267 2350 2706 3525
Size: 1133 797 51 286 83 356 819
Fragments arranged by size:
1133 819 797 356 286 83 51
BamHI G'GATC_C
Cuts at: 0 176 2397 3525
Size: 176 2221 1128
Fragments arranged by size:
2221 1128 176
BanI G'GyrC_C
Cuts at: 0 408 418 1300 1338 1389 1531 1569 1842
Size: 408 10 882 38 51 142 38 273
Cuts at: 1842 2447 2620 3189 3525
Size: 605 173 569 336
Fragments arranged by size:
882 605 569 408 336 273 173 142
51 38 38 10
BanII G_rGCy'C
Cuts at: 0 1015 1073 1213 1368 1634 1814 1838 1973
Size: 1015 58 140 155 266 180 24 135
Cuts at: 1973 2468 2640 3253 3525
Size: 495 172 613 272
Fragments arranged by size:
1015 613 495 272 266 180 172 155
140 135 58 24
BbeI G_GCGC'C
Cuts at: 0 2624 3193 3525
Size: 2624 569 332
Fragments arranged by size:
2624 569 332
BbsI GAAGACnn'nnnn_
Cuts at: 0 1051 2566 2775 3126 3525
Size: 1051 1515 209 351 399
Fragments arranged by size:
1515 1051 399 351 209
BbvCI CC'TCA_GC
Cuts at: 0 82 595 1666 2458 3307 3525
Size: 82 513 1071 792 849 218
Fragments arranged by size:
1071 849 792 513 218 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 450 484 3525
Size: 3 34 413 34 3041
Fragments arranged by size:
3041 413 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 0 423 3525
Size: 423 3102
BclI T'GATC_A
Cuts at: 0 644 2303 3525
Size: 644 1659 1222
Fragments arranged by size:
1659 1222 644
BglI GCCn_nnn'nGGC
Cuts at: 0 985 1062 1629 2545 2644 3525
Size: 985 77 567 916 99 881
Fragments arranged by size:
985 916 881 567 99 77
BglII A'GATC_T
Cuts at: 0 2127 3525
Size: 2127 1398
BlpI GC'TnA_GC
Cuts at: 0 109 2974 2989 3525
Size: 109 2865 15 536
Fragments arranged by size:
2865 536 109 15
Bme1580I G_kGCm'C
Cuts at: 0 926 1073 1426 1534 1574 1634 1994 2081
Size: 926 147 353 108 40 60 360 87
Cuts at: 2081 2468 2530 2640 2735 2984 3224 3525
Size: 387 62 110 95 249 240 301
Fragments arranged by size:
926 387 360 353 301 249 240 147
110 108 95 87 62 60 40
BmgBI CAC'GTC
Cuts at: 0 153 1155 1557 3525
Size: 153 1002 402 1968
Fragments arranged by size:
1968 1002 402 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 2808 3525
Size: 2808 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 586 894 1660 1759 2107 3406 3525
Size: 370 216 308 766 99 348 1299 119
Fragments arranged by size:
1299 766 370 348 308 216 119 99
Bpu10I CC'TnA_GC
Cuts at: 0 82 595 1666 2327 2458 2552 3247 3307
Size: 82 513 1071 661 131 94 695 60
Cuts at: 3307 3525
Size: 218
Fragments arranged by size:
1071 695 661 513 218 131 94 82
60
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1788 2697 3525
Size: 508 1280 909 828
Fragments arranged by size:
1280 909 828 508
BsaBI GATnn'nnATC
Cuts at: 0 1357 3525
Size: 1357 2168
BsaHI Gr'CG_yC
Cuts at: 0 572 2621 3190 3525
Size: 572 2049 569 335
Fragments arranged by size:
2049 572 569 335
BsaWI w'CCGG_w
Cuts at: 0 40 654 3525
Size: 40 614 2871
Fragments arranged by size:
2871 614 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 469 1285 1846 3525
Size: 78 391 816 561 1679
Fragments arranged by size:
1679 816 561 391 78
BseYI C'CCAG_C
Cuts at: 0 172 437 518 671 1065 1730 1814 2080
Size: 172 265 81 153 394 665 84 266
Cuts at: 2080 2096 3059 3345 3525
Size: 16 963 286 180
Fragments arranged by size:
963 665 394 286 266 265 180 172
153 84 81 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1100 1170 3049 3525
Size: 340 760 70 1879 476
Fragments arranged by size:
1879 760 476 340 70
BsiEI CG_ry'CG
Cuts at: 0 3205 3525
Size: 3205 320
BsiHKAI G_wGCw'C
Cuts at: 0 1015 1213 1426 1647 2915 2990 3253 3525
Size: 1015 198 213 221 1268 75 263 272
Fragments arranged by size:
1268 1015 272 263 221 213 198 75
BsmI GAATG_Cn'
Cuts at: 0 1031 1588 3525
Size: 1031 557 1937
Fragments arranged by size:
1937 1031 557
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2609 3525
Size: 2609 916
Bsp1286I G_dGCh'C
Cuts at: 0 926 1015 1073 1213 1368 1426 1534 1574
Size: 926 89 58 140 155 58 108 40
Cuts at: 1574 1634 1647 1814 1838 1973 1994 2081 2468
Size: 60 13 167 24 135 21 87 387
Cuts at: 2468 2530 2640 2735 2915 2984 2990 3224 3253
Size: 62 110 95 180 69 6 234 29
Cuts at: 3253 3525
Size: 272
Fragments arranged by size:
926 387 272 234 180 167 155 140
135 110 108 95 89 87 69 62
60 58 58 40 29 24 21 13
6
BspHI T'CATG_A
Cuts at: 0 2153 2915 3525
Size: 2153 762 610
Fragments arranged by size:
2153 762 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1404 1663 2252 2873 3116 3525
Size: 1404 259 589 621 243 409
Fragments arranged by size:
1404 621 589 409 259 243
BsrBI CCG'CTC
Cuts at: 0 560 581 2331 3207 3525
Size: 560 21 1750 876 318
Fragments arranged by size:
1750 876 560 318 21
BsrDI GCAATG_nn'
Cuts at: 0 1832 3525
Size: 1832 1693
BsrFI r'CCGG_y
Cuts at: 0 40 654 878 988 1091 1670 1749 3022
Size: 40 614 224 110 103 579 79 1273
Cuts at: 3022 3200 3325 3511 3525
Size: 178 125 186 14
Fragments arranged by size:
1273 614 579 224 186 178 125 110
103 79 40 14
BsrGI T'GTAC_A
Cuts at: 0 809 2801 3525
Size: 809 1992 724
Fragments arranged by size:
1992 809 724
BssSI C'ACGA_G
Cuts at: 0 22 3525
Size: 22 3503
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1466 3525
Size: 1466 2059
BstEII G'GTnAC_C
Cuts at: 0 1167 1377 2860 3018 3354 3525
Size: 1167 210 1483 158 336 171
Fragments arranged by size:
1483 1167 336 210 171 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 955 2451 2517 2886 2904 3525
Size: 955 1496 66 369 18 621
Fragments arranged by size:
1496 955 621 369 66 18
BstYI r'GATC_y
Cuts at: 0 75 176 2127 2397 3525
Size: 75 101 1951 270 1128
Fragments arranged by size:
1951 1128 270 101 75
Bsu36I CC'TnA_GG
Cuts at: 0 3520 3525
Size: 3520 5
BtgI C'CryG_G
Cuts at: 0 130 552 588 1382 1688 1700 2423 2735
Size: 130 422 36 794 306 12 723 312
Cuts at: 2735 3064 3128 3525
Size: 329 64 397
Fragments arranged by size:
794 723 422 397 329 312 306 130
64 36 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 711 3525
Size: 711 2814
BtsI GCAGTG_nn'
Cuts at: 0 1051 1169 1477 1793 2065 2915 3525
Size: 1051 118 308 316 272 850 610
Fragments arranged by size:
1051 850 610 316 308 272 118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2613 2648 3525
Size: 2613 35 877
Fragments arranged by size:
2613 877 35
DraIII CAC_nnn'GTG
Cuts at: 0 156 3525
Size: 156 3369
EaeI y'GGCC_r
Cuts at: 0 381 555 880 986 1649 1912 2054 2714
Size: 381 174 325 106 663 263 142 660
Cuts at: 2714 2876 2956 3092 3202 3482 3496 3525
Size: 162 80 136 110 280 14 29
Fragments arranged by size:
663 660 381 325 280 263 174 162
142 136 110 106 80 29 14
EagI C'GGCC_G
Cuts at: 0 3202 3525
Size: 3202 323
EarI CTCTTCn'nnn_
Cuts at: 0 196 310 490 890 1111 1739 1881 3525
Size: 196 114 180 400 221 628 142 1644
Fragments arranged by size:
1644 628 400 221 196 180 142 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3187 3525
Size: 3187 338
EcoICRI GAG'CTC
Cuts at: 0 1013 1211 3251 3525
Size: 1013 198 2040 274
Fragments arranged by size:
2040 1013 274 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 586 705 894 1063 1597 1660 1759
Size: 370 216 119 189 169 534 63 99
Cuts at: 1759 2107 2188 2575 2662 3406 3525
Size: 348 81 387 87 744 119
Fragments arranged by size:
744 534 387 370 348 216 189 169
119 119 99 87 81 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 1540 2521 3289 3525
Size: 487 1053 981 768 236
Fragments arranged by size:
1053 981 768 487 236
EcoO109I rG'GnC_Cy
Cuts at: 0 50 1069 1070 1115 1200 1630 1631 1734
Size: 50 1019 1 45 85 430 1 103
Cuts at: 1734 1772 2207 2514 2636 2637 3374 3525
Size: 38 435 307 122 1 737 151
Fragments arranged by size:
1019 737 435 430 307 151 122 103
85 50 45 38 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3474 3525
Size: 3474 51
FspI TGC'GCA
Cuts at: 0 1483 3525
Size: 1483 2042
HaeII r_GCGC'y
Cuts at: 0 501 2624 2757 2938 2971 3193 3244 3307
Size: 501 2123 133 181 33 222 51 63
Cuts at: 3307 3459 3525
Size: 152 66
Fragments arranged by size:
2123 501 222 181 152 133 66 63
51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1139 1171 2425 2457 3525
Size: 1139 32 1254 32 1068
Fragments arranged by size:
1254 1139 1068 32 32
HindIII A'AGCT_T
Cuts at: 0 2587 3525
Size: 2587 938
KasI G'GCGC_C
Cuts at: 0 2620 3189 3525
Size: 2620 569 336
Fragments arranged by size:
2620 569 336
KpnI G_GTAC'C
Cuts at: 0 412 3525
Size: 412 3113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 3525
Size: 178 3347
MscI TGG'CCA
Cuts at: 0 1651 1914 2056 2716 2878 3525
Size: 1651 263 142 660 162 647
Fragments arranged by size:
1651 660 647 263 162 142
MslI CAynn'nnrTG
Cuts at: 0 865 1036 1417 1687 1897 2380 2902 3217
Size: 865 171 381 270 210 483 522 315
Cuts at: 3217 3525
Size: 308
Fragments arranged by size:
865 522 483 381 315 308 270 210
171
MspA1I CmG'CkG
Cuts at: 0 113 675 1509 1818 2029 2254 2462 3300
Size: 113 562 834 309 211 225 208 838
Cuts at: 3300 3468 3504 3525
Size: 168 36 21
Fragments arranged by size:
838 834 562 309 225 211 208 168
113 36 21
NaeI GCC'GGC
Cuts at: 0 880 990 1672 1751 3202 3513 3525
Size: 880 110 682 79 1451 311 12
Fragments arranged by size:
1451 880 682 311 110 79 12
NarI GG'CG_CC
Cuts at: 0 2621 3190 3525
Size: 2621 569 335
Fragments arranged by size:
2621 569 335
NcoI C'CATG_G
Cuts at: 0 130 552 588 1700 2735 3064 3525
Size: 130 422 36 1112 1035 329 461
Fragments arranged by size:
1112 1035 461 422 329 130 36
NgoMIV G'CCGG_C
Cuts at: 0 878 988 1670 1749 3200 3511 3525
Size: 878 110 682 79 1451 311 14
Fragments arranged by size:
1451 878 682 311 110 79 14
NspI r_CATG'y
Cuts at: 0 2610 3525
Size: 2610 915
PflMI CCAn_nnn'nTGG
Cuts at: 0 678 1782 3525
Size: 678 1104 1743
Fragments arranged by size:
1743 1104 678
PfoI T'CCnGG_A
Cuts at: 0 545 602 1676 1736 3525
Size: 545 57 1074 60 1789
Fragments arranged by size:
1789 1074 545 60 57
PpuMI rG'GwC_Cy
Cuts at: 0 50 1115 1734 1772 2207 2514 3525
Size: 50 1065 619 38 435 307 1011
Fragments arranged by size:
1065 1011 619 435 307 50 38
PshAI GACnn'nnGTC
Cuts at: 0 1777 3525
Size: 1777 1748
PspOMI G'GGCC_C
Cuts at: 0 1069 1630 2464 2636 3525
Size: 1069 561 834 172 889
Fragments arranged by size:
1069 889 834 561 172
PspXI vC'TCGA_Gb
Cuts at: 0 2706 3525
Size: 2706 819
PstI C_TGCA'G
Cuts at: 0 1508 1758 1823 1889 2018 2249 2870 3008
Size: 1508 250 65 66 129 231 621 138
Cuts at: 3008 3174 3293 3525
Size: 166 119 232
Fragments arranged by size:
1508 621 250 232 231 166 138 129
119 66 65
PvuII CAG'CTG
Cuts at: 0 675 1509 1818 2029 3468 3525
Size: 675 834 309 211 1439 57
Fragments arranged by size:
1439 834 675 309 211 57
SacI G_AGCT'C
Cuts at: 0 1015 1213 3253 3525
Size: 1015 198 2040 272
Fragments arranged by size:
2040 1015 272 198
SanDI GG'GwC_CC
Cuts at: 0 50 1115 1772 3525
Size: 50 1065 657 1753
Fragments arranged by size:
1753 1065 657 50
SapI GCTCTTCn'nnn_
Cuts at: 0 196 310 1739 3525
Size: 196 114 1429 1786
Fragments arranged by size:
1786 1429 196 114
SbfI CC_TGCA'GG
Cuts at: 0 3293 3525
Size: 3293 232
ScaI AGT'ACT
Cuts at: 0 2596 3525
Size: 2596 929
SexAI A'CCwGG_T
Cuts at: 0 533 1373 2885 3525
Size: 533 840 1512 640
Fragments arranged by size:
1512 840 640 533
SfcI C'TryA_G
Cuts at: 0 1308 1504 1754 1819 1885 2014 2245 2581
Size: 1308 196 250 65 66 129 231 336
Cuts at: 2581 2866 3004 3170 3289 3525
Size: 285 138 166 119 236
Fragments arranged by size:
1308 336 285 250 236 231 196 166
138 129 119 66 65
SfoI GGC'GCC
Cuts at: 0 2622 3191 3525
Size: 2622 569 334
Fragments arranged by size:
2622 569 334
SgrAI Cr'CCGG_yG
Cuts at: 0 1091 3525
Size: 1091 2434
SmaI CCC'GGG
Cuts at: 0 1135 2269 3525
Size: 1135 1134 1256
Fragments arranged by size:
1256 1135 1134
SmlI C'TyrA_G
Cuts at: 0 2350 2706 3525
Size: 2350 356 819
Fragments arranged by size:
2350 819 356
SrfI GCCC'GGGC
Cuts at: 0 1135 3525
Size: 1135 2390
StuI AGG'CCT
Cuts at: 0 718 1936 3525
Size: 718 1218 1589
Fragments arranged by size:
1589 1218 718
StyI C'CwwG_G
Cuts at: 0 130 552 588 1700 2510 2735 3064 3182
Size: 130 422 36 1112 810 225 329 118
Cuts at: 3182 3525
Size: 343
Fragments arranged by size:
1112 810 422 343 329 225 130 118
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 891 1490 2335 2848 3525
Size: 891 599 845 513 677
Fragments arranged by size:
891 845 677 599 513
TatI w'GTAC_w
Cuts at: 0 809 2594 2789 2801 3525
Size: 809 1785 195 12 724
Fragments arranged by size:
1785 809 724 195 12
Tth111I GACn'n_nGTC
Cuts at: 0 571 847 2887 3525
Size: 571 276 2040 638
Fragments arranged by size:
2040 638 571 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 376 397 419 3525
Size: 376 21 22 3106
Fragments arranged by size:
3106 376 22 21
XhoI C'TCGA_G
Cuts at: 0 2350 2706 3525
Size: 2350 356 819
Fragments arranged by size:
2350 819 356
XmaI C'CCGG_G
Cuts at: 0 1133 2267 3525
Size: 1133 1134 1258
Fragments arranged by size:
1258 1134 1133
XmnI GAAnn'nnTTC
Cuts at: 0 2491 3457 3525
Size: 2491 966 68
Fragments arranged by size:
2491 966 68
ZraI GAC'GTC
Cuts at: 0 573 3525
Size: 573 2952
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SrfI
StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AloI ApoI AscI AseI AsiSI
AvrII BaeI BfrBI BmtI BplI BpuEI BsaAI BsaXI
BsiWI BspEI BssHII BstBI BstZ17I ClaI DraI DrdI
EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacII
SalI SfiI SgfI SnaBI SpeI SphI SspI SwaI
TstI XbaI