Restriction map for
pFN21AB9495
(Circular) (Six-base) MAPSORT of: pFN21AB9495.seq Check: 7014 from: 1 to: 7213
pFN21AB9495 7213 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2585 3366 2585
Size: 781 6432
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5023 278
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 4556 1937
Size: 1647 972 4594
Fragments arranged by size:
4594 1647 972
Acc65I G'GTAC_C
Cuts at: 4534 4534
Size: 7213
AclI AA'CG_TT
Cuts at: 1563 5340 5713 1563
Size: 3777 373 3063
Fragments arranged by size:
3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 2493 3329 3776 4353 5276 6660 6928
Size: 834 27 836 447 577 923 1384 268
Cuts at: 6928 1632
Size: 1917
Fragments arranged by size:
1917 1384 923 836 834 577 447 268
27
AfeI AGC'GCT
Cuts at: 3439 3439
Size: 7213
AflII C'TTAA_G
Cuts at: 820 1017 3179 820
Size: 197 2162 4854
Fragments arranged by size:
4854 2162 197
AflIII A'CryG_T
Cuts at: 1203 6112 7089 1203
Size: 4909 977 1327
Fragments arranged by size:
4909 1327 977
AgeI A'CCGG_T
Cuts at: 6044 6044
Size: 7213
AhdI GACnn_n'nnGTC
Cuts at: 2466 2607 4276 5942 2466
Size: 141 1669 1666 3737
Fragments arranged by size:
3737 1669 1666 141
AleI CACnn'nnGTG
Cuts at: 1166 4217 1166
Size: 3051 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3317 3408 1184
Size: 2133 91 4989
Fragments arranged by size:
4989 2133 91
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 2294
Size: 32 7181
AlwNI CAG_nnn'CTG
Cuts at: 2577 3332 3520 3784 4375 4956 6528 2577
Size: 755 188 264 591 581 1572 3262
Fragments arranged by size:
3262 1572 755 591 581 264 188
ApaI G_GGCC'C
Cuts at: 3918 4087 3918
Size: 169 7044
ApaLI G'TGCA_C
Cuts at: 5270 6426 5270
Size: 1156 6057
ApoI r'AATT_y
Cuts at: 1493 3184 4522 4748 4900 7009 1493
Size: 1691 1338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 1338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5767 160
Size: 872 4735 1606
Fragments arranged by size:
4735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2084 2136 3145 3685 3917 4538
Size: 73 115 26 52 1009 540 232 621
Cuts at: 4538 1870
Size: 4545
Fragments arranged by size:
4545 1009 621 540 232 115 73 52
26
BamHI G'GATC_C
Cuts at: 2513 4543 4910 2513
Size: 2030 367 4816
Fragments arranged by size:
4816 2030 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2216 2228 3301 4534
Size: 325 217 627 263 166 12 1073 1233
Cuts at: 4534 5989 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1233 1073 627 325 263 217
166 12
BanII G_rGCy'C
Cuts at: 1391 2085 2846 3145 3638 3918 4087 4532 1391
Size: 694 761 299 493 280 169 445 4072
Fragments arranged by size:
4072 761 694 493 445 299 280 169
BbeI G_GCGC'C
Cuts at: 2054 2220 2232 2054
Size: 166 12 7035
Fragments arranged by size:
7035 166 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 928
Size: 971 786 5456
Fragments arranged by size:
5456 971 786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2276 2310 5404 5438 1903
Size: 34 339 34 3094 34 3678
Fragments arranged by size:
3678 3094 339 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3210 3853 5105 6315 3210
Size: 643 1252 1210 4108
Fragments arranged by size:
4108 1252 1210 643
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2089 2159
Size: 107 122 71 631 605 355 62 70
Cuts at: 2159 2168 2693 3327 3552 4163 5824 136
Size: 9 525 634 225 611 1661 1525
Fragments arranged by size:
1661 1525 634 631 611 605 525 355
225 122 107 71 70 62 9
BglII A'GATC_T
Cuts at: 2638 7208 2638
Size: 4570 2643
BlpI GC'TnA_GC
Cuts at: 3441 4630 3441
Size: 1189 6024
Bme1580I G_kGCm'C
Cuts at: 1790 3306 3918 4087 5274 6430 1790
Size: 1516 612 169 1187 1156 2573
Fragments arranged by size:
2573 1516 1187 1156 612 169
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2808 3670
Size: 416 53 83 399 24 865 526 862
Cuts at: 3670 3693 4354 4944 5902 442
Size: 23 661 590 958 1753
Fragments arranged by size:
1753 958 865 862 661 590 526 416
399 83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 4170 1056
Size: 3114 4099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2198 2321 2477 3671 3733 3761 3806
Size: 821 138 123 156 1194 62 28 45
Cuts at: 3806 5873 1239
Size: 2067 2579
Fragments arranged by size:
2579 2067 1194 821 156 138 123 62
45 28
Bpu10I CC'TnA_GC
Cuts at: 2479 4011 2479
Size: 1532 5681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2626 3860 4442 5338 6203 6501 2626
Size: 1234 582 896 865 298 3338
Fragments arranged by size:
3338 1234 896 865 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5876 882
Size: 617 4377 2219
Fragments arranged by size:
4377 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4162 5004 493
Size: 711 2958 842 2702
Fragments arranged by size:
2958 2702 842 711
BsaBI GATnn'nnATC
Cuts at: 2754 2802 7207 2754
Size: 48 4405 2760
Fragments arranged by size:
4405 2760 48
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2217 2229 3555
Size: 53 83 186 936 518 166 12 1326
Cuts at: 3555 3735 5020 5402 6037 275
Size: 180 1285 382 635 1451
Fragments arranged by size:
1451 1326 1285 936 635 518 382 186
180 166 83 53 12
BsaWI w'CCGG_w
Cuts at: 5646 6044 6318 6465 6876 5646
Size: 398 274 147 411 5983
Fragments arranged by size:
5983 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 2294 2324 3118
Size: 30 165 30 762 30 99 30 794
Cuts at: 3118 3148 3889 3919 1178
Size: 30 741 30 4472
Fragments arranged by size:
4472 794 762 741 165 99 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2333 3561 3877 4093 1186
Size: 1132 15 1228 316 216 4306
Fragments arranged by size:
4306 1228 1132 316 216 15
BseYI C'CCAG_C
Cuts at: 2261 3769 6416 6912 2261
Size: 1508 2647 496 2562
Fragments arranged by size:
2647 2562 1508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 1163
Size: 573 1122 5518
Fragments arranged by size:
5518 1122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 4053 4096 4188 4663 5424
Size: 275 186 21 2031 43 92 475 761
Cuts at: 5424 6452 1540
Size: 1028 2301
Fragments arranged by size:
2301 2031 1028 761 475 275 186 92
43 21
BsiHKAI G_wGCw'C
Cuts at: 2454 4316 5274 5359 6430 2454
Size: 1862 958 85 1071 3237
Fragments arranged by size:
3237 1862 1071 958 85
BsmI GAATG_Cn'
Cuts at: 4724 4817 4724
Size: 93 7120
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2454 2846 3145 3306 3638 3918
Size: 399 295 369 392 299 161 332 280
Cuts at: 3918 4087 4316 4532 5274 5359 6430 1391
Size: 169 229 216 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 369 332 299
295 280 229 216 169 161 85
BspEI T'CCGG_A
Cuts at: 6876 6876
Size: 7213
BspHI T'CATG_A
Cuts at: 5102 6832 5102
Size: 1730 5483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 2585 3366 4568 844
Size: 1349 392 781 1202 3489
Fragments arranged by size:
3489 1349 1202 781 392
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2143 5100 1394
Size: 429 302 18 2957 3507
Fragments arranged by size:
3507 2957 429 302 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 5708 5882 65
Size: 1183 1494 2966 174 1396
Fragments arranged by size:
2966 1494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 4032 5857 6044 1700
Size: 110 243 1979 1825 187 2869
Fragments arranged by size:
2869 1979 1825 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 3511 96
Size: 3415 3798
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7213
BssSI C'ACGA_G
Cuts at: 5273 6285 5273
Size: 1012 6201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7213
BstBI TT'CG_AA
Cuts at: 3188 6017 3188
Size: 2829 4384
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7213
BstXI CCAn_nnnn'nTGG
Cuts at: 4219 4219
Size: 7213
BstYI r'GATC_y
Cuts at: 1972 2513 2638 2927 4467 4543 4910 5295 5312
Size: 541 125 289 1540 76 367 385 17
Cuts at: 5312 6764 6850 6862 7208 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1540 1452 541 385 367 346 289
125 86 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2689 3325 3429 4076 2689
Size: 636 104 647 5826
Fragments arranged by size:
5826 647 636 104
BtgI C'CryG_G
Cuts at: 513 1065 3597 3763 3942 3982 4215 513
Size: 552 2532 166 179 40 233 3511
Fragments arranged by size:
3511 2532 552 233 179 166 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6474
Fragments arranged by size:
6474 683 56
BtsI GCAGTG_nn'
Cuts at: 3263 4729 5523 5543 3263
Size: 1466 794 20 4933
Fragments arranged by size:
4933 1466 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7178
DraI TTT'AAA
Cuts at: 4517 4517
Size: 7213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3421 6220 809
Size: 507 2105 2799 1802
Fragments arranged by size:
2799 2105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2160 3369
Size: 54 1425 50 275 18 189 141 1209
Cuts at: 3369 3564 3979 4002 4050 4185 4660 5549 7178
Size: 195 415 23 48 135 475 889 1629
Cuts at: 7178 8
Size: 43
Fragments arranged by size:
1629 1425 1209 889 475 415 275 195
189 141 135 54 50 48 43 23
18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 4050 4185 4660 1537
Size: 275 207 2031 135 475 4090
Fragments arranged by size:
4090 2031 475 275 207 135
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 2442 2880 3597 3643 3896 4062 5143
Size: 1006 83 438 717 46 253 166 1081
Cuts at: 5143 1353
Size: 3423
Fragments arranged by size:
3423 1081 1006 717 438 253 166 83
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 2186 3394 5800 6174 6320
Size: 1064 138 504 93 1208 2406 374 146
Cuts at: 6320 387
Size: 1280
Fragments arranged by size:
2406 1280 1208 1064 504 374 146 138
93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2198 2321 2466 2477 2493 3329
Size: 393 428 138 123 145 11 16 836
Cuts at: 3329 3671 3733 3761 3776 3806 4353 5276 5873
Size: 342 62 28 15 30 547 923 597
Cuts at: 5873 6660 6928 1239
Size: 787 268 1524
Fragments arranged by size:
1524 923 836 787 597 547 428 393
342 268 145 138 123 62 30 28
16 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2478 3311 3477 3668 2478
Size: 833 166 191 6023
Fragments arranged by size:
6023 833 191 166
EcoO109I rG'GnC_Cy
Cuts at: 3453 3482 4072 4083 3453
Size: 29 590 11 6583
Fragments arranged by size:
6583 590 29 11
EcoRI G'AATT_C
Cuts at: 3184 4522 4900 3184
Size: 1338 378 5497
Fragments arranged by size:
5497 1338 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3859 3859
Size: 7213
FspI TGC'GCA
Cuts at: 5719 5719
Size: 7213
HaeII r_GCGC'y
Cuts at: 1678 2054 2095 2197 2220 2232 3333 3441 6360
Size: 376 41 102 23 12 1101 108 2919
Cuts at: 6360 1678
Size: 2531
Fragments arranged by size:
2919 2531 1101 376 108 102 41 23
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 2540 2572 2593
Size: 32 135 32 880 32 56 32 21
Cuts at: 2593 2625 2672 2704 2765 2797 3208 3240 4181
Size: 32 47 32 61 32 411 32 941
Cuts at: 4181 4213 4262 4294 5854 5886 5928 5960 1373
Size: 32 49 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1560 941 880 411 135 61 56
49 47 42 32 32 32 32 32
32 32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 4557 4803 669
Size: 988 281 2619 246 3079
Fragments arranged by size:
3079 2619 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7213
HpaI GTT'AAC
Cuts at: 4803 4803
Size: 7213
KasI G'GCGC_C
Cuts at: 2050 2216 2228 2050
Size: 166 12 7035
Fragments arranged by size:
7035 166 12
KpnI G_GTAC'C
Cuts at: 4538 4538
Size: 7213
MfeI C'AATT_G
Cuts at: 2345 4812 2345
Size: 2467 4746
MluI A'CGCG_T
Cuts at: 7089 7089
Size: 7213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 3363 6327 6511 1305
Size: 1329 729 2964 184 2007
Fragments arranged by size:
2964 2007 1329 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3981 4004 10
Size: 54 1425 343 2149 23 3219
Fragments arranged by size:
3219 2149 1425 343 54 23
MslI CAynn'nnrTG
Cuts at: 518 1166 2910 4217 5171 5530 5689 518
Size: 648 1744 1307 954 359 159 2042
Fragments arranged by size:
2042 1744 1307 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 3765 3944 4629 5306 6454 6699 1606
Size: 643 1516 179 685 677 1148 245 2120
Fragments arranged by size:
2120 1516 1148 685 677 643 245 179
NaeI GCC'GGC
Cuts at: 2055 2055
Size: 7213
NarI GG'CG_CC
Cuts at: 2051 2217 2229 2051
Size: 166 12 7035
Fragments arranged by size:
7035 166 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6661
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7213
NgoMIV G'CCGG_C
Cuts at: 2053 2053
Size: 7213
NheI G'CTAG_C
Cuts at: 1052 4166 1052
Size: 3114 4099
NotI GC'GGCC_GC
Cuts at: 4660 4660
Size: 7213
NspI r_CATG'y
Cuts at: 1132 1229 3395 4571 6116 1132
Size: 97 2166 1176 1545 2229
Fragments arranged by size:
2229 2166 1545 1176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7213
PciI A'CATG_T
Cuts at: 6112 6112
Size: 7213
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3649 3784 1108
Size: 2541 135 4537
Fragments arranged by size:
4537 2541 135
PmeI GTTT'AAAC
Cuts at: 4517 4517
Size: 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2326 4187 4219 4283 4315 5282 5314 6795
Size: 32 1861 32 64 32 967 32 1481
Cuts at: 6795 6827 2294
Size: 32 2680
Fragments arranged by size:
2680 1861 1481 967 64 32 32 32
32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7213
PsiI TTA'TAA
Cuts at: 4783 4783
Size: 7213
PspOMI G'GGCC_C
Cuts at: 3914 4083 3914
Size: 169 7044
PstI C_TGCA'G
Cuts at: 830 2638 3552 4565 830
Size: 1808 914 1013 3478
Fragments arranged by size:
3478 1808 1013 914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7213
SacII CC_GC'GG
Cuts at: 3766 3945 3766
Size: 179 7034
SalI G'TCGA_C
Cuts at: 1936 4555 1936
Size: 2619 4594
SapI GCTCTTCn'nnn_
Cuts at: 2442 3643 2442
Size: 1201 6012
SbfI CC_TGCA'GG
Cuts at: 3552 4565 3552
Size: 1013 6200
ScaI AGT'ACT
Cuts at: 4181 5461 7173 4181
Size: 1280 1712 4221
Fragments arranged by size:
4221 1712 1280
SexAI A'CCwGG_T
Cuts at: 3663 3663
Size: 7213
SfcI C'TryA_G
Cuts at: 826 1045 2634 3548 3585 4561 5696 6377 6568
Size: 219 1589 914 37 976 1135 681 191
Cuts at: 6568 826
Size: 1471
Fragments arranged by size:
1589 1471 1135 976 914 681 219 191
37
SfiI GGCCn_nnn'nGGCC
Cuts at: 2159 2159
Size: 7213
SfoI GGC'GCC
Cuts at: 2052 2218 2230 2052
Size: 166 12 7035
Fragments arranged by size:
7035 166 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7213
SmaI CCC'GGG
Cuts at: 1872 2138 3147 3687 4540 1872
Size: 266 1009 540 853 4545
Fragments arranged by size:
4545 1009 853 540 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2605 3179 3875 4457 5317 6218
Size: 197 926 662 574 696 582 860 901
Cuts at: 6218 6480 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 860 696 662 582 574
262 197
SnaBI TAC'GTA
Cuts at: 493 5004 493
Size: 4511 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7213
SphI G_CATG'C
Cuts at: 1132 4571 1132
Size: 3439 3774
SspI AAT'ATT
Cuts at: 5 52 5137 5
Size: 47 5085 2081
Fragments arranged by size:
5085 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 3610 4445 1517
Size: 1170 923 835 4285
Fragments arranged by size:
4285 1170 923 835
StyI C'CwwG_G
Cuts at: 513 1065 2592 3615 3891 4652 513
Size: 552 1527 1023 276 761 3074
Fragments arranged by size:
3074 1527 1023 761 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4470 5240 5257 5410 5595 6040 325
Size: 1255 2890 770 17 153 185 445 1498
Fragments arranged by size:
2890 1498 1255 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3511 4179 5459
Size: 274 80 33 51 1443 1534 668 1280
Cuts at: 5459 7171 96
Size: 1712 138
Fragments arranged by size:
1712 1534 1443 1280 668 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3118 3150 3889 3921 7173 7205 3118
Size: 32 739 32 3252 32 3126
Fragments arranged by size:
3252 3126 739 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7213
XbaI T'CTAG_A
Cuts at: 4549 4549
Size: 7213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2979 3013 3255 2979
Size: 34 242 6937
Fragments arranged by size:
6937 242 34
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7213
XmaI C'CCGG_G
Cuts at: 1870 2136 3145 3685 4538 1870
Size: 266 1009 540 853 4545
Fragments arranged by size:
4545 1009 853 540 266
XmnI GAAnn'nnTTC
Cuts at: 5342 5342
Size: 7213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5021 276
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SrfI SwaI