(Circular) (Six-base) MAPSORT of: pFN21AB9494.seq Check: 8686 from: 1 to: 7225 pFN21AB9494 7225 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2651 2829 4108 2651 Size: 178 1279 5768 Fragments arranged by size: 5768 1279 178 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5035 278 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4568 1937 Size: 2631 4594 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 5352 5725 1563 Size: 3789 373 3063 Fragments arranged by size: 3789 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3030 5288 6672 6940 1632 Size: 1398 2258 1384 268 1917 Fragments arranged by size: 2258 1917 1398 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7028 AflIII A'CryG_T Cuts at: 1203 2607 6124 7101 1203 Size: 1404 3517 977 1327 Fragments arranged by size: 3517 1404 1327 977 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 5954 5954 Size: 7225 AleI CACnn'nnGTG Cuts at: 1166 2222 1166 Size: 1056 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7225 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 2808 Size: 32 7193 AlwNI CAG_nnn'CTG Cuts at: 3457 4294 4333 4360 4450 4453 4968 6540 3457 Size: 837 39 27 90 3 515 1572 4142 Fragments arranged by size: 4142 1572 837 515 90 39 27 3 ApaI G_GGCC'C Cuts at: 2145 3517 2145 Size: 1372 5853 ApaLI G'TGCA_C Cuts at: 4394 5282 6438 4394 Size: 888 1156 5181 Fragments arranged by size: 5181 1156 888 ApoI r'AATT_y Cuts at: 1493 2780 3998 4534 4760 4912 7021 1493 Size: 1287 1218 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 1287 1218 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 5779 160 Size: 872 4747 1606 Fragments arranged by size: 4747 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 2080 3017 3087 4211 4550 1870 Size: 73 137 937 70 1124 339 4545 Fragments arranged by size: 4545 1124 937 339 137 73 70 BamHI G'GATC_C Cuts at: 4555 4922 4555 Size: 367 6858 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 2198 3043 3113 4029 Size: 325 217 627 382 29 845 70 916 Cuts at: 4029 4144 4514 4546 6001 618 Size: 115 370 32 1455 1842 Fragments arranged by size: 1842 1455 916 845 627 382 370 325 217 115 70 32 29 BanII G_rGCy'C Cuts at: 1391 2145 2640 2834 3517 4544 1391 Size: 754 495 194 683 1027 4072 Fragments arranged by size: 4072 1027 754 683 495 194 BbeI G_GCGC'C Cuts at: 2173 4518 2173 Size: 2345 4880 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 928 Size: 971 377 5877 Fragments arranged by size: 5877 971 377 BbvCI CC'TCA_GC Cuts at: 3121 3121 Size: 7225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5416 5450 1903 Size: 34 3479 34 3678 Fragments arranged by size: 3678 3479 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6327 5117 Size: 1210 6015 BclI T'GATC_A Cuts at: 1548 3837 1548 Size: 2289 4936 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2540 4049 4153 Size: 107 122 71 631 605 868 1509 104 Cuts at: 4153 5836 136 Size: 1683 1525 Fragments arranged by size: 1683 1525 1509 868 631 605 122 107 104 71 BglII A'GATC_T Cuts at: 7220 7220 Size: 7225 BlpI GC'TnA_GC Cuts at: 3776 4439 4642 3776 Size: 663 203 6359 Fragments arranged by size: 6359 663 203 Bme1580I G_kGCm'C Cuts at: 1790 2145 2203 3018 3048 3221 3517 4032 4149 Size: 355 58 815 30 173 296 515 117 Cuts at: 4149 4398 5286 6442 1790 Size: 249 888 1156 2573 Fragments arranged by size: 2573 1156 888 815 515 355 296 249 173 117 58 30 BmgBI CAC'GTC Cuts at: 1318 2796 2950 3337 1318 Size: 1478 154 387 5206 Fragments arranged by size: 5206 1478 387 154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2346 2424 3153 Size: 416 53 83 399 24 929 78 729 Cuts at: 3153 3470 4956 5914 442 Size: 317 1486 958 1753 Fragments arranged by size: 1753 1486 958 929 729 416 399 317 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7225 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3880 3880 Size: 7225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 3479 3710 4001 4054 4316 4355 5885 Size: 1370 870 231 291 53 262 39 1530 Cuts at: 5885 1239 Size: 2579 Fragments arranged by size: 2579 1530 1370 870 291 262 231 53 39 Bpu10I CC'TnA_GC Cuts at: 3121 3121 Size: 7225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3770 5350 6215 6513 3770 Size: 1580 865 298 4482 Fragments arranged by size: 4482 1580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4417 5888 882 Size: 617 2918 1471 2219 Fragments arranged by size: 2918 2219 1471 617 BsaAI yAC'GTr Cuts at: 493 1204 3382 3535 5016 493 Size: 711 2178 153 1481 2702 Fragments arranged by size: 2702 2178 1481 711 153 BsaBI GATnn'nnATC Cuts at: 7219 7219 Size: 7225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2170 3198 4515 5032 Size: 53 83 186 936 637 1028 1317 517 Cuts at: 5032 5414 6049 275 Size: 382 635 1451 Fragments arranged by size: 1451 1317 1028 936 637 635 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 5658 6056 6330 6477 6888 4092 Size: 1566 398 274 147 411 4429 Fragments arranged by size: 4429 1566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2808 2838 3683 3713 1178 Size: 30 165 30 1405 30 845 30 4690 Fragments arranged by size: 4690 1405 845 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 3053 3077 3377 3856 1186 Size: 1480 387 24 300 479 4555 Fragments arranged by size: 4555 1480 479 387 300 24 BseYI C'CCAG_C Cuts at: 2646 2981 3138 3349 4148 4510 6428 6924 2646 Size: 335 157 211 799 362 1918 496 2947 Fragments arranged by size: 2947 1918 799 496 362 335 211 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 2921 3275 3392 3515 4334 4367 Size: 573 605 580 354 117 123 819 33 Cuts at: 4367 1163 Size: 4021 Fragments arranged by size: 4021 819 605 580 573 354 123 117 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2373 2913 3521 4436 4675 5436 Size: 275 186 372 540 608 915 239 761 Cuts at: 5436 6464 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 915 761 608 540 372 275 239 186 BsiHKAI G_wGCw'C Cuts at: 2640 3576 3795 4398 5286 5371 6442 2640 Size: 936 219 603 888 85 1071 3423 Fragments arranged by size: 3423 1071 936 888 603 219 85 BsmI GAATG_Cn' Cuts at: 4736 4829 4736 Size: 93 7132 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2203 2640 2834 3018 3048 3221 Size: 399 355 58 437 194 184 30 173 Cuts at: 3221 3517 3576 3795 4032 4149 4398 4544 5286 Size: 296 59 219 237 117 249 146 742 Cuts at: 5286 5371 6442 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 399 355 296 249 237 219 194 184 173 146 117 85 59 58 30 BspEI T'CCGG_A Cuts at: 6888 6888 Size: 7225 BspHI T'CATG_A Cuts at: 3840 5114 6844 3840 Size: 1274 1730 4221 Fragments arranged by size: 4221 1730 1274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2651 2829 3738 4108 4580 844 Size: 1807 178 909 370 472 3489 Fragments arranged by size: 3489 1807 909 472 370 178 BsrBI CCG'CTC Cuts at: 1394 1823 3067 3989 4068 5112 1394 Size: 429 1244 922 79 1044 3507 Fragments arranged by size: 3507 1244 1044 922 429 79 BsrDI GCAATG_nn' Cuts at: 65 1248 5720 5894 65 Size: 1183 4472 174 1396 Fragments arranged by size: 4472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2347 4266 5869 6056 1700 Size: 110 537 1919 1603 187 2869 Fragments arranged by size: 2869 1919 1603 537 187 110 BsrGI T'GTAC_A Cuts at: 96 2550 3204 3252 3654 96 Size: 2454 654 48 402 3667 Fragments arranged by size: 3667 2454 654 402 48 BssHII G'CGCG_C Cuts at: 1925 2061 1925 Size: 136 7089 BssSI C'ACGA_G Cuts at: 3293 5285 6297 3293 Size: 1992 1012 4221 Fragments arranged by size: 4221 1992 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3739 4321 4381 4453 794 Size: 2945 582 60 72 3566 Fragments arranged by size: 3566 2945 582 72 60 BstBI TT'CG_AA Cuts at: 2148 6029 2148 Size: 3881 3344 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7225 BstYI r'GATC_y Cuts at: 1972 2568 2673 4329 4555 4922 5307 5324 6776 Size: 596 105 1656 226 367 385 17 1452 Cuts at: 6776 6862 6874 7220 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1656 1452 596 385 367 346 226 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2536 2544 2700 2868 3611 3723 Size: 552 939 532 8 156 168 743 112 Cuts at: 3723 4034 513 Size: 311 3704 Fragments arranged by size: 3704 939 743 552 532 311 168 156 112 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2144 487 Size: 683 56 918 5568 Fragments arranged by size: 5568 918 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3793 4375 4505 4741 5535 5555 2938 Size: 855 582 130 236 794 20 4608 Fragments arranged by size: 4608 855 794 582 236 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7190 DraI TTT'AAA Cuts at: 4529 4529 Size: 7225 DraIII CAC_nnn'GTG Cuts at: 2221 2221 Size: 7225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6232 809 Size: 507 4916 1802 Fragments arranged by size: 4916 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2482 2910 Size: 54 1425 50 275 18 540 112 428 Cuts at: 2910 3518 3537 4037 4059 4672 5561 7190 8 Size: 608 19 500 22 613 889 1629 43 Fragments arranged by size: 1629 1425 889 613 608 540 500 428 275 112 54 50 43 22 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2370 2910 3518 4672 1537 Size: 275 558 540 608 1154 4090 Fragments arranged by size: 4090 1154 608 558 540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2774 3176 5155 1353 Size: 1421 402 1979 3423 Fragments arranged by size: 3423 1979 1421 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3910 5812 6186 6332 387 Size: 1064 138 2321 1902 374 146 1280 Fragments arranged by size: 2321 1902 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2638 2638 Size: 7225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 3030 3479 3710 4001 4054 4316 Size: 393 977 421 449 231 291 53 262 Cuts at: 4316 4355 5288 5885 6672 6940 1239 Size: 39 933 597 787 268 1524 Fragments arranged by size: 1524 977 933 787 597 449 421 393 291 268 262 231 53 39 EcoNI CCTnn'n_nnAGG Cuts at: 2320 2320 Size: 7225 EcoO109I rG'GnC_Cy Cuts at: 2142 2325 2340 3490 3513 2142 Size: 183 15 1150 23 5854 Fragments arranged by size: 5854 1150 183 23 15 EcoRI G'AATT_C Cuts at: 2780 3998 4534 4912 2780 Size: 1218 536 378 5093 Fragments arranged by size: 5093 1218 536 378 FspI TGC'GCA Cuts at: 4504 5731 4504 Size: 1227 5998 HaeII r_GCGC'y Cuts at: 1678 2173 2857 3862 4518 6372 1678 Size: 495 684 1005 656 1854 2531 Fragments arranged by size: 2531 1854 1005 684 656 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5866 5898 5940 5972 1373 Size: 32 135 32 4294 32 42 32 2626 Fragments arranged by size: 4294 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4201 4569 4815 669 Size: 988 281 2263 368 246 3079 Fragments arranged by size: 3079 2263 988 368 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7225 HpaI GTT'AAC Cuts at: 4815 4815 Size: 7225 KasI G'GCGC_C Cuts at: 2169 4514 2169 Size: 2345 4880 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 2616 4824 2616 Size: 2208 5017 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 6339 6523 1305 Size: 1707 3327 184 2007 Fragments arranged by size: 3327 2007 1707 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2484 3539 10 Size: 54 1425 343 652 1055 3696 Fragments arranged by size: 3696 1425 1055 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2892 2943 3650 4391 5183 5542 Size: 648 1056 670 51 707 741 792 359 Cuts at: 5542 5701 518 Size: 159 2042 Fragments arranged by size: 2042 1056 792 741 707 670 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2851 3243 3408 3697 3775 4343 4450 4641 Size: 1245 392 165 289 78 568 107 191 Cuts at: 4641 5318 6466 6711 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1245 1148 677 568 392 289 245 191 165 107 78 NarI GG'CG_CC Cuts at: 2170 4515 2170 Size: 2345 4880 NcoI C'CATG_G Cuts at: 513 1065 2004 2536 2868 513 Size: 552 939 532 332 4870 Fragments arranged by size: 4870 939 552 532 332 NdeI CA'TA_TG Cuts at: 387 387 Size: 7225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7225 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NspI r_CATG'y Cuts at: 1132 1229 2395 2611 4207 4583 6128 1132 Size: 97 1166 216 1596 376 1545 2229 Fragments arranged by size: 2229 1596 1545 1166 376 216 97 PasI CC'CwG_GG Cuts at: 1348 3483 1348 Size: 2135 5090 PciI A'CATG_T Cuts at: 2607 6124 2607 Size: 3517 3708 PflMI CCAn_nnn'nTGG Cuts at: 1108 3457 3937 1108 Size: 2349 480 4396 Fragments arranged by size: 4396 2349 480 PfoI T'CCnGG_A Cuts at: 2625 3543 3960 4332 2625 Size: 918 417 372 5518 Fragments arranged by size: 5518 918 417 372 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PmlI CAC'GTG Cuts at: 3535 3535 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2808 2840 5294 5326 6807 6839 2808 Size: 32 2454 32 1481 32 3194 Fragments arranged by size: 3194 2454 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2340 2340 Size: 7225 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7225 PsiI TTA'TAA Cuts at: 4795 4795 Size: 7225 PspOMI G'GGCC_C Cuts at: 2141 3513 2141 Size: 1372 5853 PstI C_TGCA'G Cuts at: 830 3735 3774 4275 4329 4577 830 Size: 2905 39 501 54 248 3478 Fragments arranged by size: 3478 2905 501 248 54 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 3697 3775 4343 4450 3697 Size: 78 568 107 6472 Fragments arranged by size: 6472 568 107 78 SacI G_AGCT'C Cuts at: 2640 2640 Size: 7225 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SanDI GG'GwC_CC Cuts at: 2340 2340 Size: 7225 SapI GCTCTTCn'nnn_ Cuts at: 3176 3176 Size: 7225 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 5473 7185 5473 Size: 1712 5513 SexAI A'CCwGG_T Cuts at: 3831 3831 Size: 7225 SfcI C'TryA_G Cuts at: 826 1045 3731 3770 4271 4325 4573 5708 6389 Size: 219 2686 39 501 54 248 1135 681 Cuts at: 6389 6580 826 Size: 191 1471 Fragments arranged by size: 2686 1471 1135 681 501 248 219 191 54 39 SfoI GGC'GCC Cuts at: 2171 4516 2171 Size: 2345 4880 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7225 SmaI CCC'GGG Cuts at: 1872 4552 1872 Size: 2680 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3785 5329 6230 6492 820 Size: 197 926 1842 1544 901 262 1553 Fragments arranged by size: 1842 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5016 493 Size: 4523 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 4583 1132 Size: 3451 3774 SspI AAT'ATT Cuts at: 5 52 5149 5 Size: 47 5097 2081 Fragments arranged by size: 5097 2081 47 StuI AGG'CCT Cuts at: 1517 3719 4160 1517 Size: 2202 441 4582 Fragments arranged by size: 4582 2202 441 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2536 2868 3924 4407 4664 Size: 552 939 66 466 332 1056 483 257 Cuts at: 4664 513 Size: 3074 Fragments arranged by size: 3074 1056 939 552 483 466 332 257 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2646 3152 3341 4047 5252 5269 Size: 1255 606 460 506 189 706 1205 17 Cuts at: 5269 5422 5607 6052 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1205 706 606 506 460 445 189 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2550 3204 3252 Size: 274 80 33 51 1443 573 654 48 Cuts at: 3252 3654 3708 5471 7183 96 Size: 402 54 1763 1712 138 Fragments arranged by size: 1763 1712 1443 654 573 402 274 138 80 54 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 3657 3683 3689 3715 7185 7217 2780 Size: 32 845 26 6 26 3470 32 2788 Fragments arranged by size: 3470 2788 845 32 32 26 26 6 Tth111I GACn'n_nGTC Cuts at: 1316 2114 1316 Size: 798 6427 XbaI T'CTAG_A Cuts at: 4561 4561 Size: 7225 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4138 4138 Size: 7225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7225 XmaI C'CCGG_G Cuts at: 1870 4550 1870 Size: 2680 4545 XmnI GAAnn'nnTTC Cuts at: 3851 5354 3851 Size: 1503 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5033 276 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI