(Circular) (Six-base) MAPSORT of: pFN21AB9492.seq Check: 480 from: 1 to: 8314 pFN21AB9492 8314 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6124 278 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3466 5657 1937 Size: 1529 2191 4594 Fragments arranged by size: 4594 2191 1529 Acc65I G'GTAC_C Cuts at: 5635 5635 Size: 8314 AclI AA'CG_TT Cuts at: 1563 6441 6814 1563 Size: 4878 373 3063 Fragments arranged by size: 4878 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2413 3181 3477 5507 6377 7761 8029 1632 Size: 781 768 296 2030 870 1384 268 1917 Fragments arranged by size: 2030 1917 1384 870 781 768 296 268 AflII C'TTAA_G Cuts at: 820 1017 2162 820 Size: 197 1145 6972 Fragments arranged by size: 6972 1145 197 AflIII A'CryG_T Cuts at: 1203 7213 8190 1203 Size: 6010 977 1327 Fragments arranged by size: 6010 1327 977 AgeI A'CCGG_T Cuts at: 7145 7145 Size: 8314 AhdI GACnn_n'nnGTC Cuts at: 7043 7043 Size: 8314 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8314 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5208 5240 5208 Size: 32 8282 AlwNI CAG_nnn'CTG Cuts at: 5485 5596 6057 7629 5485 Size: 111 461 1572 6170 Fragments arranged by size: 6170 1572 461 111 ApaLI G'TGCA_C Cuts at: 4029 6371 7527 4029 Size: 2342 1156 4816 Fragments arranged by size: 4816 2342 1156 ApoI r'AATT_y Cuts at: 1493 2263 2673 3425 3550 3665 3698 4725 5623 Size: 770 410 752 125 115 33 1027 898 Cuts at: 5623 5849 6001 8110 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1027 898 770 752 410 226 152 125 115 33 AseI AT'TA_AT Cuts at: 160 1032 2852 6868 160 Size: 872 1820 4016 1606 Fragments arranged by size: 4016 1820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 AvaI C'yCGr_G Cuts at: 1870 1943 5639 1870 Size: 73 3696 4545 Fragments arranged by size: 4545 3696 73 BamHI G'GATC_C Cuts at: 3040 5644 6011 3040 Size: 2604 367 5343 Fragments arranged by size: 5343 2604 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5635 7090 618 Size: 325 217 627 3848 1455 1842 Fragments arranged by size: 3848 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2161 3589 5633 1391 Size: 770 1428 2044 4072 Fragments arranged by size: 4072 2044 1428 770 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 3278 3978 4536 4868 928 Size: 971 933 446 700 558 332 4374 Fragments arranged by size: 4374 971 933 700 558 446 332 BbvCI CC'TCA_GC Cuts at: 4173 4173 Size: 8314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4885 4919 6505 6539 1903 Size: 34 2948 34 1586 34 3678 Fragments arranged by size: 3678 2948 1586 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3819 6206 7416 3819 Size: 2387 1210 4717 Fragments arranged by size: 4717 2387 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8314 BfrBI ATG'CAT Cuts at: 2880 3931 5088 2880 Size: 1051 1157 6106 Fragments arranged by size: 6106 1157 1051 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6925 136 Size: 107 122 71 631 605 5253 1525 Fragments arranged by size: 5253 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5011 8309 5011 Size: 3298 5016 BlpI GC'TnA_GC Cuts at: 2496 5149 5731 2496 Size: 2653 582 5079 Fragments arranged by size: 5079 2653 582 Bme1580I G_kGCm'C Cuts at: 1790 4033 4747 6375 7531 1790 Size: 2243 714 1628 1156 2573 Fragments arranged by size: 2573 2243 1628 1156 714 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 6045 7003 Size: 416 53 83 399 24 1145 3483 958 Cuts at: 7003 442 Size: 1753 Fragments arranged by size: 3483 1753 1145 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4133 1056 Size: 3077 5237 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2154 4874 6974 1239 Size: 836 79 2720 2100 2579 Fragments arranged by size: 2720 2579 2100 836 79 Bpu10I CC'TnA_GC Cuts at: 4173 4173 Size: 8314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3542 4212 5138 6439 7304 7602 3542 Size: 670 926 1301 865 298 4254 Fragments arranged by size: 4254 1301 926 865 670 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3785 6977 882 Size: 617 2286 3192 2219 Fragments arranged by size: 3192 2286 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6105 493 Size: 711 4901 2702 Fragments arranged by size: 4901 2702 711 BsaBI GATnn'nnATC Cuts at: 4401 8308 4401 Size: 3907 4407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6121 6503 7138 275 Size: 53 83 186 936 4588 382 635 1451 Fragments arranged by size: 4588 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 6747 7145 7419 7566 7977 4785 Size: 1962 398 274 147 411 5122 Fragments arranged by size: 5122 1962 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2388 2418 2574 2604 5208 Size: 30 165 30 985 30 156 30 2604 Cuts at: 5208 5238 1178 Size: 30 4254 Fragments arranged by size: 4254 2604 985 165 156 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2130 5130 5133 5213 1186 Size: 941 3 3000 3 80 4287 Fragments arranged by size: 4287 3000 941 80 3 3 BseYI C'CCAG_C Cuts at: 2059 2686 5346 7517 8013 2059 Size: 627 2660 2171 496 2360 Fragments arranged by size: 2660 2360 2171 627 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4713 4830 1163 Size: 573 2977 117 4647 Fragments arranged by size: 4647 2977 573 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5764 6525 7553 1540 Size: 275 186 3763 761 1028 2301 Fragments arranged by size: 3763 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4033 6375 6460 7531 4033 Size: 2342 85 1071 4816 Fragments arranged by size: 4816 2342 1071 85 BsmI GAATG_Cn' Cuts at: 2232 2455 4936 5825 5918 2232 Size: 223 2481 889 93 4628 Fragments arranged by size: 4628 2481 889 223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 3589 4033 4747 5633 6375 6460 Size: 399 371 1428 444 714 886 742 85 Cuts at: 6460 7531 1391 Size: 1071 2174 Fragments arranged by size: 2174 1428 1071 886 742 714 444 399 371 85 BspEI T'CCGG_A Cuts at: 4785 7977 4785 Size: 3192 5122 BspHI T'CATG_A Cuts at: 6203 7933 6203 Size: 1730 6584 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5301 5437 5669 844 Size: 4457 136 232 3489 Fragments arranged by size: 4457 3489 232 136 BsrBI CCG'CTC Cuts at: 1394 1823 6201 1394 Size: 429 4378 3507 Fragments arranged by size: 4378 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3375 3385 4140 6809 6983 65 Size: 1183 2127 10 755 2669 174 1396 Fragments arranged by size: 2669 2127 1396 1183 755 174 10 BsrFI r'CCGG_y Cuts at: 1700 1810 6958 7145 1700 Size: 110 5148 187 2869 Fragments arranged by size: 5148 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2304 3979 96 Size: 2208 1675 4431 Fragments arranged by size: 4431 2208 1675 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8314 BssSI C'ACGA_G Cuts at: 6374 7386 6374 Size: 1012 7302 BstAPI GCAn_nnn'nTGC Cuts at: 794 3506 794 Size: 2712 5602 BstBI TT'CG_AA Cuts at: 7118 7118 Size: 8314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8314 BstXI CCAn_nnnn'nTGG Cuts at: 2625 5384 2625 Size: 2759 5555 BstYI r'GATC_y Cuts at: 1972 3011 3040 5011 5644 6011 6396 6413 7865 Size: 1039 29 1971 633 367 385 17 1452 Cuts at: 7865 7951 7963 8309 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1971 1452 1039 633 385 367 346 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3103 4614 513 Size: 552 939 1099 1511 4213 Fragments arranged by size: 4213 1511 1099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7575 Fragments arranged by size: 7575 683 56 BtsI GCAGTG_nn' Cuts at: 2362 2396 2981 5830 6624 6644 2362 Size: 34 585 2849 794 20 4032 Fragments arranged by size: 4032 2849 794 585 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8279 DraI TTT'AAA Cuts at: 3220 5238 5618 3220 Size: 2018 380 5916 Fragments arranged by size: 5916 2018 380 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7321 809 Size: 507 6005 1802 Fragments arranged by size: 6005 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3912 5280 5761 Size: 54 1425 50 275 18 2082 1368 481 Cuts at: 5761 6650 8279 8 Size: 889 1629 43 Fragments arranged by size: 2082 1629 1425 1368 889 481 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5761 1537 Size: 275 3949 4090 Fragments arranged by size: 4090 3949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3368 4055 4404 5186 6244 1353 Size: 2015 687 349 782 1058 3423 Fragments arranged by size: 3423 2015 1058 782 687 349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5052 6901 7275 7421 387 Size: 1064 138 3463 1849 374 146 1280 Fragments arranged by size: 3463 1849 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2154 2413 3181 3477 4874 5507 Size: 393 443 79 259 768 296 1397 633 Cuts at: 5507 6377 6974 7761 8029 1239 Size: 870 597 787 268 1524 Fragments arranged by size: 1524 1397 870 787 768 633 597 443 393 296 268 259 79 EcoNI CCTnn'n_nnAGG Cuts at: 3219 3219 Size: 8314 EcoO109I rG'GnC_Cy Cuts at: 5130 5509 5130 Size: 379 7935 EcoRI G'AATT_C Cuts at: 2263 3698 4725 5623 6001 2263 Size: 1435 1027 898 378 4576 Fragments arranged by size: 4576 1435 1027 898 378 EcoRV GAT'ATC Cuts at: 4216 4216 Size: 8314 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3265 3718 4896 3265 Size: 453 1178 6683 Fragments arranged by size: 6683 1178 453 FspI TGC'GCA Cuts at: 6820 6820 Size: 8314 HaeII r_GCGC'y Cuts at: 1678 4009 7461 1678 Size: 2331 3452 2531 Fragments arranged by size: 3452 2531 2331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 3055 3087 3726 Size: 32 135 32 592 32 859 32 639 Cuts at: 3726 3758 3916 3948 6955 6987 7029 7061 1373 Size: 32 158 32 3007 32 42 32 2626 Fragments arranged by size: 3007 2626 859 639 592 158 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2104 5658 5904 669 Size: 988 281 166 3554 246 3079 Fragments arranged by size: 3554 3079 988 281 246 166 HindIII A'AGCT_T Cuts at: 748 2609 748 Size: 1861 6453 HpaI GTT'AAC Cuts at: 5904 5904 Size: 8314 KpnI G_GTAC'C Cuts at: 5639 5639 Size: 8314 MfeI C'AATT_G Cuts at: 3938 4946 5913 3938 Size: 1008 967 6339 Fragments arranged by size: 6339 1008 967 MluI A'CGCG_T Cuts at: 8190 8190 Size: 8314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5200 7428 7612 1305 Size: 3895 2228 184 2007 Fragments arranged by size: 3895 2228 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3914 5282 10 Size: 54 1425 343 2082 1368 3042 Fragments arranged by size: 3042 2082 1425 1368 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2478 2623 2875 6272 6631 6790 518 Size: 648 1312 145 252 3397 359 159 2042 Fragments arranged by size: 3397 2042 1312 648 359 252 159 145 MspA1I CmG'CkG Cuts at: 1606 2154 3503 4112 4705 5350 5730 6407 7555 Size: 548 1349 609 593 645 380 677 1148 Cuts at: 7555 7800 1606 Size: 245 2120 Fragments arranged by size: 2120 1349 1148 677 645 609 593 548 380 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3103 4614 513 Size: 552 939 1099 1511 4213 Fragments arranged by size: 4213 1511 1099 939 552 NdeI CA'TA_TG Cuts at: 387 5002 387 Size: 4615 3699 NheI G'CTAG_C Cuts at: 1052 4129 1052 Size: 3077 5237 NotI GC'GGCC_GC Cuts at: 5761 5761 Size: 8314 NsiI A_TGCA'T Cuts at: 2882 3933 5090 2882 Size: 1051 1157 6106 Fragments arranged by size: 6106 1157 1051 NspI r_CATG'y Cuts at: 1132 1229 2686 3864 4144 4817 4900 5672 7217 Size: 97 1457 1178 280 673 83 772 1545 Cuts at: 7217 1132 Size: 2229 Fragments arranged by size: 2229 1545 1457 1178 772 673 280 97 83 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8314 PciI A'CATG_T Cuts at: 7213 7213 Size: 8314 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8314 PfoI T'CCnGG_A Cuts at: 3681 5376 3681 Size: 1695 6619 PmeI GTTT'AAAC Cuts at: 5618 5618 Size: 8314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5208 5240 6383 6415 7896 7928 5208 Size: 32 1143 32 1481 32 5594 Fragments arranged by size: 5594 1481 1143 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5130 5130 Size: 8314 PshAI GACnn'nnGTC Cuts at: 1613 2865 1613 Size: 1252 7062 PsiI TTA'TAA Cuts at: 5884 5884 Size: 8314 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5282 5314 5282 Size: 32 8282 PstI C_TGCA'G Cuts at: 830 2153 4021 4117 4704 5666 830 Size: 1323 1868 96 587 962 3478 Fragments arranged by size: 3478 1868 1323 962 587 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8314 PvuII CAG'CTG Cuts at: 3503 4112 4705 5350 3503 Size: 609 593 645 6467 Fragments arranged by size: 6467 645 609 593 SalI G'TCGA_C Cuts at: 1936 5656 1936 Size: 3720 4594 SapI GCTCTTCn'nnn_ Cuts at: 3368 3368 Size: 8314 SbfI CC_TGCA'GG Cuts at: 5666 5666 Size: 8314 ScaI AGT'ACT Cuts at: 6562 8274 6562 Size: 1712 6602 SexAI A'CCwGG_T Cuts at: 5403 5403 Size: 8314 SfcI C'TryA_G Cuts at: 826 1045 2149 3467 4017 4113 4700 5542 5662 Size: 219 1104 1318 550 96 587 842 120 Cuts at: 5662 6797 7478 7669 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1318 1135 1104 842 681 587 550 219 191 120 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8314 SmaI CCC'GGG Cuts at: 1872 5641 1872 Size: 3769 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 3557 4227 5117 6418 7319 Size: 197 926 219 1395 670 890 1301 901 Cuts at: 7319 7581 820 Size: 262 1553 Fragments arranged by size: 1553 1395 1301 926 901 890 670 262 219 197 SnaBI TAC'GTA Cuts at: 493 6105 493 Size: 5612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8314 SphI G_CATG'C Cuts at: 1132 5672 1132 Size: 4540 3774 SspI AAT'ATT Cuts at: 5 52 2226 2380 4514 6238 5 Size: 47 2174 154 2134 1724 2081 Fragments arranged by size: 2174 2134 2081 1724 154 47 StuI AGG'CCT Cuts at: 1517 3225 1517 Size: 1708 6606 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 3103 3392 4614 4629 4639 Size: 552 939 213 886 289 1222 15 10 Cuts at: 4639 5512 5563 5753 513 Size: 873 51 190 3074 Fragments arranged by size: 3074 1222 939 886 873 552 289 213 190 51 15 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2678 3018 6341 6358 6511 6696 7141 Size: 1255 1098 340 3323 17 153 185 445 Cuts at: 7141 325 Size: 1498 Fragments arranged by size: 3323 1498 1255 1098 445 340 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2395 2443 Size: 274 80 33 51 1443 327 91 48 Cuts at: 2443 3979 4428 6560 8272 96 Size: 1536 449 2132 1712 138 Fragments arranged by size: 2132 1712 1536 1443 449 327 274 138 91 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4327 4359 4716 4748 8274 8306 4327 Size: 32 357 32 3526 32 4335 Fragments arranged by size: 4335 3526 357 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8314 XbaI T'CTAG_A Cuts at: 2270 2914 5650 2270 Size: 644 2736 4934 Fragments arranged by size: 4934 2736 644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8314 XmaI C'CCGG_G Cuts at: 1870 5639 1870 Size: 3769 4545 XmnI GAAnn'nnTTC Cuts at: 2280 3418 6443 2280 Size: 1138 3025 4151 Fragments arranged by size: 4151 3025 1138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6122 276 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI