(Circular) (Six-base) MAPSORT of: pFN21AB9488.seq Check: 7435 from: 1 to: 8104 pFN21AB9488 8104 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5914 278 Size: 53 83 186 936 4378 2468 Fragments arranged by size: 4378 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2200 5371 5447 1937 Size: 263 3171 76 4594 Fragments arranged by size: 4594 3171 263 76 Acc65I G'GTAC_C Cuts at: 5425 5425 Size: 8104 AclI AA'CG_TT Cuts at: 1563 6231 6604 1563 Size: 4668 373 3063 Fragments arranged by size: 4668 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 2686 2756 3287 3524 3857 4044 4050 Size: 819 235 70 531 237 333 187 6 Cuts at: 4050 6167 7551 7819 1632 Size: 2117 1384 268 1917 Fragments arranged by size: 2117 1917 1384 819 531 333 268 237 235 187 70 6 AflII C'TTAA_G Cuts at: 820 1017 4756 820 Size: 197 3739 4168 Fragments arranged by size: 4168 3739 197 AflIII A'CryG_T Cuts at: 1203 7003 7980 1203 Size: 5800 977 1327 Fragments arranged by size: 5800 1327 977 AgeI A'CCGG_T Cuts at: 6935 6935 Size: 8104 AhdI GACnn_n'nnGTC Cuts at: 3595 6833 3595 Size: 3238 4866 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 6907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2503 1184 Size: 1319 6785 AlwNI CAG_nnn'CTG Cuts at: 3694 5272 5847 7419 3694 Size: 1578 575 1572 4379 Fragments arranged by size: 4379 1578 1572 575 ApaI G_GGCC'C Cuts at: 4777 4777 Size: 8104 ApaLI G'TGCA_C Cuts at: 6161 7317 6161 Size: 1156 6948 ApoI r'AATT_y Cuts at: 1493 2639 3249 3708 4682 5145 5413 5639 5791 Size: 1146 610 459 974 463 268 226 152 Cuts at: 5791 7900 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1146 974 610 463 459 268 226 152 AseI AT'TA_AT Cuts at: 160 1032 6658 160 Size: 872 5626 1606 Fragments arranged by size: 5626 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8104 AvaI C'yCGr_G Cuts at: 1870 1943 4835 5429 1870 Size: 73 2892 594 4545 Fragments arranged by size: 4545 2892 594 73 BamHI G'GATC_C Cuts at: 5434 5801 5434 Size: 367 7737 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 5425 6880 618 Size: 325 217 627 494 3144 1455 1842 Fragments arranged by size: 3144 1842 1455 627 494 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2782 4777 4800 5160 5423 1391 Size: 621 770 1995 23 360 263 4072 Fragments arranged by size: 4072 1995 770 621 360 263 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4421 4889 928 Size: 971 2522 468 4143 Fragments arranged by size: 4143 2522 971 468 BbvCI CC'TCA_GC Cuts at: 4972 4972 Size: 8104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6295 6329 1903 Size: 34 4358 34 3678 Fragments arranged by size: 4358 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2199 3141 5216 5255 5996 7206 2199 Size: 942 2075 39 741 1210 3097 Fragments arranged by size: 3097 2075 1210 942 741 39 BclI T'GATC_A Cuts at: 1548 4242 1548 Size: 2694 5410 BfrBI ATG'CAT Cuts at: 2495 2954 2495 Size: 459 7645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4157 6715 136 Size: 107 122 71 631 605 2485 2558 1525 Fragments arranged by size: 2558 2485 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2835 5059 8099 2835 Size: 2224 3040 2840 Fragments arranged by size: 3040 2840 2224 BlpI GC'TnA_GC Cuts at: 5521 5521 Size: 8104 Bme1580I G_kGCm'C Cuts at: 1790 3350 4777 6165 7321 1790 Size: 1560 1427 1388 1156 2573 Fragments arranged by size: 2573 1560 1427 1388 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8104 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 4219 5154 Size: 416 53 83 399 24 909 1893 935 Cuts at: 5154 5835 6793 442 Size: 681 958 1753 Fragments arranged by size: 1893 1753 958 935 909 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8104 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4784 4890 6764 1239 Size: 3545 106 1874 2579 Fragments arranged by size: 3545 2579 1874 106 Bpu10I CC'TnA_GC Cuts at: 4185 4972 5327 4185 Size: 787 355 6962 Fragments arranged by size: 6962 787 355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3948 4827 6229 7094 7392 3948 Size: 879 1402 865 298 4660 Fragments arranged by size: 4660 1402 879 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5189 6767 882 Size: 617 3690 1578 2219 Fragments arranged by size: 3690 2219 1578 617 BsaAI yAC'GTr Cuts at: 493 1204 4423 5023 5204 5895 493 Size: 711 3219 600 181 691 2702 Fragments arranged by size: 3219 2702 711 691 600 181 BsaBI GATnn'nnATC Cuts at: 3831 8098 3831 Size: 4267 3837 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2118 5911 6293 6928 Size: 53 83 186 936 585 3793 382 635 Cuts at: 6928 275 Size: 1451 Fragments arranged by size: 3793 1451 936 635 585 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6537 6935 7209 7356 7767 6537 Size: 398 274 147 411 6874 Fragments arranged by size: 6874 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2472 5193 5223 1178 Size: 30 165 30 1039 30 2721 30 4059 Fragments arranged by size: 4059 2721 1039 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4134 4137 4140 1186 Size: 2948 3 3 5150 Fragments arranged by size: 5150 2948 3 3 BseYI C'CCAG_C Cuts at: 7307 7803 7307 Size: 496 7608 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2949 3788 4278 1163 Size: 573 1213 839 490 4989 Fragments arranged by size: 4989 1213 839 573 490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5554 6315 7343 1540 Size: 275 186 3553 761 1028 2301 Fragments arranged by size: 3553 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 3512 4707 4800 6165 6250 7321 2172 Size: 1340 1195 93 1365 85 1071 2955 Fragments arranged by size: 2955 1365 1340 1195 1071 93 85 BsmI GAATG_Cn' Cuts at: 4892 5059 5615 5708 4892 Size: 167 556 93 7288 Fragments arranged by size: 7288 556 167 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4079 4079 Size: 8104 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2172 2782 3350 3512 4707 4777 Size: 399 222 160 610 568 162 1195 70 Cuts at: 4777 4800 5160 5423 6165 6250 7321 1391 Size: 23 360 263 742 85 1071 2174 Fragments arranged by size: 2174 1195 1071 742 610 568 399 360 263 222 162 160 85 70 23 BspEI T'CCGG_A Cuts at: 7767 7767 Size: 8104 BspHI T'CATG_A Cuts at: 5993 7723 5993 Size: 1730 6374 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5459 844 Size: 4615 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3082 5991 1394 Size: 429 1259 2909 3507 Fragments arranged by size: 3507 2909 1259 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 2497 3322 4087 4983 6599 6773 Size: 1183 1212 37 825 765 896 1616 174 Cuts at: 6773 65 Size: 1396 Fragments arranged by size: 1616 1396 1212 1183 896 825 765 174 37 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 6748 6935 1700 Size: 110 236 4702 187 2869 Fragments arranged by size: 4702 2869 236 187 110 BsrGI T'GTAC_A Cuts at: 96 2764 4270 96 Size: 2668 1506 3930 Fragments arranged by size: 3930 2668 1506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8104 BssSI C'ACGA_G Cuts at: 6164 7176 6164 Size: 1012 7092 BstAPI GCAn_nnn'nTGC Cuts at: 794 3071 5230 794 Size: 2277 2159 3668 Fragments arranged by size: 3668 2277 2159 BstBI TT'CG_AA Cuts at: 6908 6908 Size: 8104 BstEII G'GTnAC_C Cuts at: 1184 2177 1184 Size: 993 7111 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2981 2365 Size: 616 7488 BstYI r'GATC_y Cuts at: 1972 2241 2835 4066 5059 5434 5801 6186 6203 Size: 269 594 1231 993 375 367 385 17 Cuts at: 6203 7655 7741 7753 8099 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1231 993 594 385 375 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7365 Fragments arranged by size: 7365 683 56 BtsI GCAGTG_nn' Cuts at: 2418 3776 5620 6414 6434 2418 Size: 1358 1844 794 20 4088 Fragments arranged by size: 4088 1844 1358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8104 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8069 DraI TTT'AAA Cuts at: 4107 5408 4107 Size: 1301 6803 DraIII CAC_nnn'GTG Cuts at: 2019 2548 2019 Size: 529 7575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 7111 809 Size: 507 3017 2778 1802 Fragments arranged by size: 3017 2778 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5551 6440 8069 Size: 54 1425 50 275 18 3721 889 1629 Cuts at: 8069 8 Size: 43 Fragments arranged by size: 3721 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5551 1537 Size: 275 3739 4090 Fragments arranged by size: 4090 3739 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2614 3312 4434 6034 1353 Size: 1261 698 1122 1600 3423 Fragments arranged by size: 3423 1600 1261 1122 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6691 7065 7211 387 Size: 1064 138 5102 374 146 1280 Fragments arranged by size: 5102 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4798 4798 Size: 8104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2686 2756 3287 3524 3857 4044 Size: 393 819 235 70 531 237 333 187 Cuts at: 4044 4050 4784 4890 6167 6764 7551 7819 1239 Size: 6 734 106 1277 597 787 268 1524 Fragments arranged by size: 1524 1277 819 787 734 597 531 393 333 268 237 235 187 106 70 6 EcoO109I rG'GnC_Cy Cuts at: 4774 4968 4774 Size: 194 7910 EcoRI G'AATT_C Cuts at: 3249 5413 5791 3249 Size: 2164 378 5562 Fragments arranged by size: 5562 2164 378 EcoRV GAT'ATC Cuts at: 3667 3667 Size: 8104 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5143 5143 Size: 8104 FspI TGC'GCA Cuts at: 6610 6610 Size: 8104 HaeII r_GCGC'y Cuts at: 1678 7251 1678 Size: 5573 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 2442 2474 3581 Size: 32 135 32 556 32 282 32 1107 Cuts at: 3581 3613 5051 5083 5190 5222 5372 5404 6745 Size: 32 1438 32 107 32 150 32 1341 Cuts at: 6745 6777 6819 6851 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1438 1341 1107 556 282 150 135 107 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 5372 5448 5694 669 Size: 988 281 1080 2354 76 246 3079 Fragments arranged by size: 3079 2354 1080 988 281 246 76 HindIII A'AGCT_T Cuts at: 748 3117 3417 748 Size: 2369 300 5435 Fragments arranged by size: 5435 2369 300 HpaI GTT'AAC Cuts at: 3018 5694 3018 Size: 2676 5428 KpnI G_GTAC'C Cuts at: 5429 5429 Size: 8104 MfeI C'AATT_G Cuts at: 2877 4647 5703 2877 Size: 1770 1056 5278 Fragments arranged by size: 5278 1770 1056 MluI A'CGCG_T Cuts at: 7980 7980 Size: 8104 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4468 7218 7402 1305 Size: 3163 2750 184 2007 Fragments arranged by size: 3163 2750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6282 Fragments arranged by size: 6282 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2221 2363 3807 4632 5052 6062 6421 Size: 648 1055 142 1444 825 420 1010 359 Cuts at: 6421 6580 518 Size: 159 2042 Fragments arranged by size: 2042 1444 1055 1010 825 648 420 359 159 142 MspA1I CmG'CkG Cuts at: 1606 3265 4376 4513 5272 5323 5520 6197 7345 Size: 1659 1111 137 759 51 197 677 1148 Cuts at: 7345 7590 1606 Size: 245 2120 Fragments arranged by size: 2120 1659 1148 1111 759 677 245 197 137 51 NaeI GCC'GGC Cuts at: 2048 2048 Size: 8104 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7552 NdeI CA'TA_TG Cuts at: 387 2218 387 Size: 1831 6273 NgoMIV G'CCGG_C Cuts at: 2046 2046 Size: 8104 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8104 NotI GC'GGCC_GC Cuts at: 5551 5551 Size: 8104 NsiI A_TGCA'T Cuts at: 2497 2956 2497 Size: 459 7645 NspI r_CATG'y Cuts at: 1132 1229 2499 2591 5462 7007 1132 Size: 97 1270 92 2871 1545 2229 Fragments arranged by size: 2871 2229 1545 1270 97 92 PasI CC'CwG_GG Cuts at: 1348 4770 1348 Size: 3422 4682 PciI A'CATG_T Cuts at: 7003 7003 Size: 8104 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 2548 4913 1108 Size: 911 529 2365 4299 Fragments arranged by size: 4299 2365 911 529 PmeI GTTT'AAAC Cuts at: 5408 5408 Size: 8104 PmlI CAC'GTG Cuts at: 4423 5023 4423 Size: 600 7504 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6173 6205 7686 7718 6173 Size: 32 1481 32 6559 Fragments arranged by size: 6559 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4968 4968 Size: 8104 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8104 PsiI TTA'TAA Cuts at: 2091 2561 2825 4223 5674 2091 Size: 470 264 1398 1451 4521 Fragments arranged by size: 4521 1451 1398 470 264 PspOMI G'GGCC_C Cuts at: 4773 4773 Size: 8104 PspXI vC'TCGA_Gb Cuts at: 4835 4835 Size: 8104 PstI C_TGCA'G Cuts at: 830 2482 5404 5456 830 Size: 1652 2922 52 3478 Fragments arranged by size: 3478 2922 1652 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8104 PvuII CAG'CTG Cuts at: 3265 4513 5272 5323 3265 Size: 1248 759 51 6046 Fragments arranged by size: 6046 1248 759 51 SacI G_AGCT'C Cuts at: 4800 4800 Size: 8104 SalI G'TCGA_C Cuts at: 1936 5370 5446 1936 Size: 3434 76 4594 Fragments arranged by size: 4594 3434 76 SbfI CC_TGCA'GG Cuts at: 5456 5456 Size: 8104 ScaI AGT'ACT Cuts at: 6352 8064 6352 Size: 1712 6392 SfcI C'TryA_G Cuts at: 826 1045 2478 2839 3495 5111 5400 5452 6587 Size: 219 1433 361 656 1616 289 52 1135 Cuts at: 6587 7268 7459 826 Size: 681 191 1471 Fragments arranged by size: 1616 1471 1433 1135 681 656 361 289 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8104 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8104 SmaI CCC'GGG Cuts at: 1872 5431 1872 Size: 3559 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3963 4756 4835 4842 6208 7109 Size: 197 926 2020 793 79 7 1366 901 Cuts at: 7109 7371 820 Size: 262 1553 Fragments arranged by size: 2020 1553 1366 926 901 793 262 197 79 7 SnaBI TAC'GTA Cuts at: 493 5204 5895 493 Size: 4711 691 2702 Fragments arranged by size: 4711 2702 691 SpeI A'CTAG_T Cuts at: 152 152 Size: 8104 SphI G_CATG'C Cuts at: 1132 5462 1132 Size: 4330 3774 SspI AAT'ATT Cuts at: 5 52 2794 3392 3730 4921 6028 5 Size: 47 2742 598 338 1191 1107 2081 Fragments arranged by size: 2742 2081 1191 1107 598 338 47 StuI AGG'CCT Cuts at: 1517 2978 1517 Size: 1461 6643 StyI C'CwwG_G Cuts at: 513 1065 3562 5543 513 Size: 552 2497 1981 3074 Fragments arranged by size: 3074 2497 1981 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3720 4155 5138 6131 6148 6301 6486 Size: 1255 2140 435 983 993 17 153 185 Cuts at: 6486 6931 325 Size: 445 1498 Fragments arranged by size: 2140 1498 1255 993 983 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2524 2764 3745 Size: 274 80 33 51 1443 547 240 981 Cuts at: 3745 4270 6350 8062 96 Size: 525 2080 1712 138 Fragments arranged by size: 2080 1712 1443 981 547 525 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4083 4115 4465 4497 8064 8096 4083 Size: 32 350 32 3567 32 4091 Fragments arranged by size: 4091 3567 350 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8104 XbaI T'CTAG_A Cuts at: 5440 5440 Size: 8104 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4822 4822 Size: 8104 XhoI C'TCGA_G Cuts at: 1943 4835 1943 Size: 2892 5212 XmaI C'CCGG_G Cuts at: 1870 5429 1870 Size: 3559 4545 XmnI GAAnn'nnTTC Cuts at: 6233 6233 Size: 8104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5912 276 Size: 53 83 186 936 4378 2468 Fragments arranged by size: 4378 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI PfoI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI