Restriction map for
pFN21AB9483
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AcyI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BbeI BbrPI BbvCI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BsmFI BsmI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI BstEII
BstPI BstXI BstYI CelII Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FseI
HaeII Hin1I KasI KpnI KspI
MamI MfeI MflI MluI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I Tth111I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 2553 GTATAC GT^[AC][GT]AC
AccII 91 CGCG CG^CG
AccIII 15 TCCGGA T^CCGGA
AclI 860 AACGTT AA^CGTT
AcsI 1953 AAATTT [GA]^AATT[CT]
AhdI 1714 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1465 GATCC GGATC(4/5)
AocI 1316 CCTTAGG CC^T[ACGT]AGG
ApaI 2335 GGGCCC GGGCC^C
ApoI 1953 AAATTT [GA]^AATT[CT]
AspEI 1714 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 482 CTCGAG C^[CT]CG[GA]G
AvaII 582 GGACC G^G[AT]CC
AviII 627 TGCGCA TGC^GCA
BalI 806 TGGCCA TGG^CCA
BanI 1093 GGCACC G^G[CT][GA]CC
BbsI 104 GTCTTC GAAGAC(2/6)
BcgI 2184 GCAAGCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1572 GGATAC GTATCC(6/5)
BclI 795 TGATCA T^GATCA
BpuAI 104 GTCTTC GAAGAC(2/6)
BsaAI 2330 TACGTG [CT]AC^GT[GA]
BseAI 15 TCCGGA T^CCGGA
BsoBI 482 CTCGAG C^[CT]CG[GA]G
BspDI 2315 ATCGAT AT^CGAT
BspEI 15 TCCGGA T^CCGGA
BspMI 1129 ACCTGC ACCTGC(4/8)
Bst1107I 2553 GTATAC GTA^TAC
BstBI 61 TTCGAA TT^CGAA
BstUI 91 CGCG CG^CG
BstZ17I 2553 GTATAC GTA^TAC
Bsu36I 1316 CCTTAGG CC^T[ACGT]AGG
ClaI 2315 ATCGAT AT^CGAT
DrdI 196 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 806 TGGCCA [CT]^GGCC[GA]
Eam1105I 1714 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1316 CCTTAGG CC^T[ACGT]AGG
EcoT22I 2138 ATGCAT ATGCA^T
FbaI 795 TGATCA T^GATCA
FspI 627 TGCGCA TGC^GCA
HgaI 1766 GCGTC GACGC(5/10)
HincII 985 GTTAAC GT[CT]^[GA]AC
HindII 985 GTTAAC GT[CT]^[GA]AC
HpaI 985 GTTAAC GTT^AAC
MluNI 806 TGGCCA TGG^CCA
MroI 15 TCCGGA T^CCGGA
MscI 806 TGGCCA TGG^CCA
MslI 1378 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 91 CGCG CG^CG
NdeI 2248 CATATG CA^TATG
NsiI 2138 ATGCAT ATGCA^T
NspV 61 TTCGAA TT^CGAA
PaeR7I 482 CTCGAG C^TCGAG
PflMI 847 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 860 AACGTT AA^CGTT
SfcI 2217 CTATAG C^T[GA][CT]AG
SfuI 61 TTCGAA TT^CGAA
SmlI 482 CTCGAG C^T[CT][GA]AG
SpeI 1233 ACTAGT A^CTAGT
SphI 2163 GCATGC GCATG^C
SwaI 632 ATTTAAAT ATTT^AAAT
Van91I 847 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 482 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1414 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1504 CAGGAACTG
Asp700I 1285 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2022 GAACATTTTC
BpmI 1655 CTGGAG CTGGAG(16/14)
2425 CTGGAG
BsaI 1262 GAGACC GGTCTC(1/5)
451 GGTCTC
BsaWI 15 TCCGGA [AT]^CCGG[AT]
2530 ACCGGA
BsgI 1131 CTGCAC GTGCAG(16/14)
1496 CTGCAC
Bsp1407I 1188 TGTACA T^GTACA
2292 TGTACA
BsrGI 1188 TGTACA T^GTACA
2292 TGTACA
CfoI 628 GCGC GCG^C
2167 GCGC
EcoRV 1159 GATATC GAT^ATC
1638 GATATC
FauI 89 CCCGC CCCGC(4/6)
352 GCGGG
HapII 16 CCGG C^CGG
2531 CCGG
HhaI 628 GCGC GCG^C
2167 GCGC
HinP1I 628 GCGC G^CGC
2167 GCGC
HpaII 16 CCGG C^CGG
2531 CCGG
MspI 16 CCGG C^CGG
2531 CCGG
NcoI 809 CCATGG C^CATGG
974 CCATGG
NspI 2163 GCATGC [GA]CATG^[CT]
2280 ACATGC
PleI 148 GACTC GAGTC(4/5)
1720 GAGTC
SacI 440 GAGCTC GAGCT^C
2428 GAGCTC
SspBI 1188 TGTACA T^GTACA
2292 TGTACA
XmnI 1285 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2022 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BfaI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I