(Circular) (Six-base) MAPSORT of: pFN21AB9483.seq Check: 7967 from: 1 to: 7276 pFN21AB9483 7276 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5086 278 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4559 4619 1937 Size: 2622 60 4594 Fragments arranged by size: 4594 2622 60 Acc65I G'GTAC_C Cuts at: 4597 4597 Size: 7276 AclI AA'CG_TT Cuts at: 1563 2866 5403 5776 1563 Size: 1303 2537 373 3063 Fragments arranged by size: 3063 2537 1303 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2157 2178 3072 3276 3309 3398 3536 Size: 464 61 21 894 204 33 89 138 Cuts at: 3536 5339 6723 6991 1632 Size: 1803 1384 268 1917 Fragments arranged by size: 1917 1803 1384 894 464 268 204 138 89 61 33 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7079 AflIII A'CryG_T Cuts at: 1203 6175 7152 1203 Size: 4972 977 1327 Fragments arranged by size: 4972 1327 977 AgeI A'CCGG_T Cuts at: 6107 6107 Size: 7276 AhdI GACnn_n'nnGTC Cuts at: 3724 6005 3724 Size: 2281 4995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7276 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2370 1184 Size: 1186 6090 AlwNI CAG_nnn'CTG Cuts at: 3424 3514 5019 6591 3424 Size: 90 1505 1572 4109 Fragments arranged by size: 4109 1572 1505 90 ApaI G_GGCC'C Cuts at: 4344 4344 Size: 7276 ApaLI G'TGCA_C Cuts at: 5333 6489 5333 Size: 1156 6120 ApoI r'AATT_y Cuts at: 1493 3958 4585 4811 4963 7072 1493 Size: 2465 627 226 152 2109 1697 Fragments arranged by size: 2465 2109 1697 627 226 152 AseI AT'TA_AT Cuts at: 160 1032 5830 160 Size: 872 4798 1606 Fragments arranged by size: 4798 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 AvaI C'yCGr_G Cuts at: 1870 1943 2487 4601 1870 Size: 73 544 2114 4545 Fragments arranged by size: 4545 2114 544 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4315 4348 4315 Size: 33 7243 BamHI G'GATC_C Cuts at: 4606 4973 4606 Size: 367 6909 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3098 4597 6052 618 Size: 325 217 627 1311 1499 1455 1842 Fragments arranged by size: 1842 1499 1455 1311 627 325 217 BanII G_rGCy'C Cuts at: 1391 2449 3631 4344 4437 4595 1391 Size: 1058 1182 713 93 158 4072 Fragments arranged by size: 4072 1182 1058 713 158 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 928 Size: 971 203 6102 Fragments arranged by size: 6102 971 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4178 4212 5467 5501 1903 Size: 34 2241 34 1255 34 3678 Fragments arranged by size: 3678 2241 1255 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3571 5168 6378 3571 Size: 1597 1210 4469 Fragments arranged by size: 4469 1597 1210 BclI T'GATC_A Cuts at: 1548 2800 1548 Size: 1252 6024 BfrBI ATG'CAT Cuts at: 4145 4145 Size: 7276 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5887 136 Size: 107 122 71 631 605 4215 1525 Fragments arranged by size: 4215 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7271 7271 Size: 7276 BlpI GC'TnA_GC Cuts at: 4693 4693 Size: 7276 Bme1580I G_kGCm'C Cuts at: 1790 4344 5337 6493 1790 Size: 2554 993 1156 2573 Fragments arranged by size: 2573 2554 1156 993 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7276 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2640 5007 5965 Size: 416 53 83 399 24 1223 2367 958 Cuts at: 5965 442 Size: 1753 Fragments arranged by size: 2367 1753 1223 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7276 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 4305 2578 Size: 1727 5549 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3681 4451 5936 1239 Size: 2442 770 1485 2579 Fragments arranged by size: 2579 2442 1485 770 Bpu10I CC'TnA_GC Cuts at: 2343 2343 Size: 7276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5401 6266 6564 5401 Size: 865 298 6113 Fragments arranged by size: 6113 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2462 3261 5939 882 Size: 617 963 799 2678 2219 Fragments arranged by size: 2678 2219 963 799 617 BsaAI yAC'GTr Cuts at: 493 1204 4337 5067 493 Size: 711 3133 730 2702 Fragments arranged by size: 3133 2702 730 711 BsaBI GATnn'nnATC Cuts at: 7270 7270 Size: 7276 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5083 5465 6100 275 Size: 53 83 186 936 3550 382 635 1451 Fragments arranged by size: 3550 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2020 4535 5709 6107 6381 6528 6939 2020 Size: 2515 1174 398 274 147 411 2357 Fragments arranged by size: 2515 2357 1174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7051 Fragments arranged by size: 7051 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2309 2327 2330 2333 2336 2339 2342 Size: 889 234 18 3 3 3 3 3 Cuts at: 2342 2505 2532 1186 Size: 163 27 5930 Fragments arranged by size: 5930 889 234 163 27 18 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3424 4017 6479 6975 3424 Size: 593 2462 496 3725 Fragments arranged by size: 3725 2462 593 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3121 3486 1163 Size: 573 1385 365 4953 Fragments arranged by size: 4953 1385 573 365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4726 5487 6515 1540 Size: 275 186 2725 761 1028 2301 Fragments arranged by size: 2725 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2449 3765 4437 5337 5422 6493 2449 Size: 1316 672 900 85 1071 3232 Fragments arranged by size: 3232 1316 1071 900 672 85 BsmI GAATG_Cn' Cuts at: 4787 4880 4787 Size: 93 7183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2449 3631 3765 4344 4437 4595 5337 Size: 399 659 1182 134 579 93 158 742 Cuts at: 5337 5422 6493 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1182 1071 742 659 579 399 158 134 93 85 BspEI T'CCGG_A Cuts at: 2020 6939 2020 Size: 4919 2357 BspHI T'CATG_A Cuts at: 5165 6895 5165 Size: 1730 5546 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 4631 844 Size: 2299 1488 3489 Fragments arranged by size: 3489 2299 1488 BsrBI CCG'CTC Cuts at: 1394 1823 5163 1394 Size: 429 3340 3507 Fragments arranged by size: 3507 3340 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2609 3624 3721 5771 5945 65 Size: 1183 1361 1015 97 2050 174 1396 Fragments arranged by size: 2050 1396 1361 1183 1015 174 97 BsrFI r'CCGG_y Cuts at: 1700 1810 5920 6107 1700 Size: 110 4110 187 2869 Fragments arranged by size: 4110 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3193 4297 96 Size: 3097 1104 3075 Fragments arranged by size: 3097 3075 1104 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7276 BssSI C'ACGA_G Cuts at: 5336 6348 5336 Size: 1012 6264 BstAPI GCAn_nnn'nTGC Cuts at: 794 3491 3500 3755 794 Size: 2697 9 255 4315 Fragments arranged by size: 4315 2697 255 9 BstBI TT'CG_AA Cuts at: 2067 6080 2067 Size: 4013 3263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7276 BstYI r'GATC_y Cuts at: 1972 4606 4973 5358 5375 6827 6913 6925 7271 Size: 2634 367 385 17 1452 86 12 346 Cuts at: 7271 1972 Size: 1977 Fragments arranged by size: 2634 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4560 4560 Size: 7276 Bsu36I CC'TnA_GG Cuts at: 3322 3322 Size: 7276 BtgI C'CryG_G Cuts at: 513 1065 2814 2979 4372 513 Size: 552 1749 165 1393 3417 Fragments arranged by size: 3417 1749 1393 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2376 4058 487 Size: 683 56 1150 1682 3705 Fragments arranged by size: 3705 1682 1150 683 56 BtsI GCAGTG_nn' Cuts at: 2352 4531 4792 5586 5606 2352 Size: 2179 261 794 20 4022 Fragments arranged by size: 4022 2179 794 261 20 ClaI AT'CG_AT Cuts at: 1554 4321 1554 Size: 2767 4509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3366 3401 529 Size: 35 2802 35 4404 Fragments arranged by size: 4404 2802 35 35 DraI TTT'AAA Cuts at: 2640 2838 3315 3609 4580 2640 Size: 198 477 294 971 5336 Fragments arranged by size: 5336 971 477 294 198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2207 6283 809 Size: 507 891 4076 1802 Fragments arranged by size: 4076 1802 891 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2811 4723 5612 Size: 54 1425 50 275 18 981 1912 889 Cuts at: 5612 7241 8 Size: 1629 43 Fragments arranged by size: 1912 1629 1425 981 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4723 1537 Size: 275 2911 4090 Fragments arranged by size: 4090 2911 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2295 2304 2496 3102 4480 5206 1353 Size: 723 219 9 192 606 1378 726 3423 Fragments arranged by size: 3423 1378 726 723 606 219 192 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2089 5863 6237 6383 387 Size: 1064 138 500 3774 374 146 1280 Fragments arranged by size: 3774 1280 1064 500 374 146 138 EcoICRI GAG'CTC Cuts at: 2447 4435 2447 Size: 1988 5288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2157 2178 3072 3276 3309 3398 Size: 393 464 61 21 894 204 33 89 Cuts at: 3398 3536 3681 4451 5339 5936 6723 6991 1239 Size: 138 145 770 888 597 787 268 1524 Fragments arranged by size: 1524 894 888 787 770 597 464 393 268 204 145 138 89 61 33 21 EcoO109I rG'GnC_Cy Cuts at: 2968 4341 4426 2968 Size: 1373 85 5818 Fragments arranged by size: 5818 1373 85 EcoRI G'AATT_C Cuts at: 4585 4963 4585 Size: 378 6898 EcoRV GAT'ATC Cuts at: 3166 3645 3166 Size: 479 6797 FspI TGC'GCA Cuts at: 2634 5782 2634 Size: 3148 4128 HaeII r_GCGC'y Cuts at: 1678 6423 1678 Size: 4745 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2785 2817 3689 3710 3721 Size: 32 135 32 1213 32 872 21 11 Cuts at: 3721 3742 5917 5949 5991 6023 1373 Size: 21 2175 32 42 32 2626 Fragments arranged by size: 2626 2175 1213 872 135 42 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2992 4620 4866 669 Size: 988 281 1054 1628 246 3079 Fragments arranged by size: 3079 1628 1054 988 281 246 HindIII A'AGCT_T Cuts at: 748 2949 3036 3291 748 Size: 2201 87 255 4733 Fragments arranged by size: 4733 2201 255 87 HpaI GTT'AAC Cuts at: 2992 4866 2992 Size: 1874 5402 KpnI G_GTAC'C Cuts at: 4601 4601 Size: 7276 MfeI C'AATT_G Cuts at: 4875 4875 Size: 7276 MluI A'CGCG_T Cuts at: 7152 7152 Size: 7276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6390 6574 1305 Size: 5085 184 2007 Fragments arranged by size: 5085 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 10 Size: 54 1425 343 981 4473 Fragments arranged by size: 4473 1425 981 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3387 5234 5593 5752 518 Size: 648 2221 1847 359 159 2042 Fragments arranged by size: 2221 2042 1847 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2878 3002 3424 4692 5369 6517 6762 Size: 529 743 124 422 1268 677 1148 245 Cuts at: 6762 1606 Size: 2120 Fragments arranged by size: 2120 1268 1148 743 677 529 422 245 124 NcoI C'CATG_G Cuts at: 513 1065 2814 2979 513 Size: 552 1749 165 4810 Fragments arranged by size: 4810 1749 552 165 NdeI CA'TA_TG Cuts at: 387 4254 387 Size: 3867 3409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7276 NotI GC'GGCC_GC Cuts at: 4723 4723 Size: 7276 NsiI A_TGCA'T Cuts at: 4147 4147 Size: 7276 NspI r_CATG'y Cuts at: 1132 1229 4172 4289 4634 6179 1132 Size: 97 2943 117 345 1545 2229 Fragments arranged by size: 2943 2229 1545 345 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7276 PciI A'CATG_T Cuts at: 6175 6175 Size: 7276 PflMI CCAn_nnn'nTGG Cuts at: 1108 2858 1108 Size: 1750 5526 PmeI GTTT'AAAC Cuts at: 4580 4580 Size: 7276 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5345 5377 6858 6890 5345 Size: 32 1481 32 5731 Fragments arranged by size: 5731 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7276 PsiI TTA'TAA Cuts at: 4846 4846 Size: 7276 PspOMI G'GGCC_C Cuts at: 4340 4340 Size: 7276 PspXI vC'TCGA_Gb Cuts at: 2487 2487 Size: 7276 PstI C_TGCA'G Cuts at: 830 4628 830 Size: 3798 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7276 PvuII CAG'CTG Cuts at: 2135 2878 3002 3424 2135 Size: 743 124 422 5987 Fragments arranged by size: 5987 743 422 124 SacI G_AGCT'C Cuts at: 2449 4437 2449 Size: 1988 5288 SalI G'TCGA_C Cuts at: 1936 4618 1936 Size: 2682 4594 SbfI CC_TGCA'GG Cuts at: 4628 4628 Size: 7276 ScaI AGT'ACT Cuts at: 5524 7236 5524 Size: 1712 5564 SfcI C'TryA_G Cuts at: 826 1045 4222 4572 4624 5759 6440 6631 826 Size: 219 3177 350 52 1135 681 191 1471 Fragments arranged by size: 3177 1471 1135 681 350 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7276 SmaI CCC'GGG Cuts at: 1872 4603 1872 Size: 2731 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2487 5380 6281 6543 820 Size: 197 926 544 2893 901 262 1553 Fragments arranged by size: 2893 1553 926 901 544 262 197 SnaBI TAC'GTA Cuts at: 493 5067 493 Size: 4574 2702 SpeI A'CTAG_T Cuts at: 152 3238 152 Size: 3086 4190 SphI G_CATG'C Cuts at: 1132 4172 4634 1132 Size: 3040 462 3774 Fragments arranged by size: 3774 3040 462 SspI AAT'ATT Cuts at: 5 52 2701 3753 3881 5200 5 Size: 47 2649 1052 128 1319 2081 Fragments arranged by size: 2649 2081 1319 1052 128 47 StuI AGG'CCT Cuts at: 1517 2425 3320 4239 1517 Size: 908 895 919 4554 Fragments arranged by size: 4554 919 908 895 StyI C'CwwG_G Cuts at: 513 1065 2590 2814 2979 4715 513 Size: 552 1525 224 165 1736 3074 Fragments arranged by size: 3074 1736 1525 552 224 165 SwaI ATTT'AAAT Cuts at: 2640 2640 Size: 7276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 2786 5303 5320 5473 5658 6103 Size: 1255 873 333 2517 17 153 185 445 Cuts at: 6103 325 Size: 1498 Fragments arranged by size: 2517 1498 1255 873 445 333 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3193 4297 5522 Size: 274 80 33 51 1443 1216 1104 1225 Cuts at: 5522 7234 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1225 1216 1104 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3776 3808 4170 4202 7236 7268 3776 Size: 32 362 32 3034 32 3784 Fragments arranged by size: 3784 3034 362 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7276 XbaI T'CTAG_A Cuts at: 4612 4612 Size: 7276 XhoI C'TCGA_G Cuts at: 1943 2487 1943 Size: 544 6732 XmaI C'CCGG_G Cuts at: 1870 4601 1870 Size: 2731 4545 XmnI GAAnn'nnTTC Cuts at: 3294 4031 5405 3294 Size: 737 1374 5165 Fragments arranged by size: 5165 1374 737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5084 276 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BsiWI BsmBI BstXI DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI