Restriction map for
pFN21AB9483
(Linear) (Six-base) MAPSORT of: KIBB9483.seq Check: 6407 from: 1 to: 2571
KIBB9483.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 2554 2571
Size: 2554 17
AclI AA'CG_TT
Cuts at: 0 861 2571
Size: 861 1710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 152 173 1067 1271 1304 1393 1531
Size: 91 61 21 894 204 33 89 138
Cuts at: 1531 2571
Size: 1040
Fragments arranged by size:
1040 894 204 138 91 89 61 33
21
AhdI GACnn_n'nnGTC
Cuts at: 0 1719 2571
Size: 1719 852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 2571
Size: 365 2206
AlwNI CAG_nnn'CTG
Cuts at: 0 1419 1509 2571
Size: 1419 90 1062
Fragments arranged by size:
1419 1062 90
ApaI G_GGCC'C
Cuts at: 0 2339 2571
Size: 2339 232
ApoI r'AATT_y
Cuts at: 0 1953 2571
Size: 1953 618
AvaI C'yCGr_G
Cuts at: 0 482 2571
Size: 482 2089
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2310 2343 2571
Size: 2310 33 228
Fragments arranged by size:
2310 228 33
BanI G'GyrC_C
Cuts at: 0 1093 2571
Size: 1093 1478
BanII G_rGCy'C
Cuts at: 0 444 1626 2339 2432 2571
Size: 444 1182 713 93 139
Fragments arranged by size:
1182 713 444 139 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 2571
Size: 97 2474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2173 2207 2571
Size: 2173 34 364
Fragments arranged by size:
2173 364 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1566 2571
Size: 1566 1005
BclI T'GATC_A
Cuts at: 0 795 2571
Size: 795 1776
BfrBI ATG'CAT
Cuts at: 0 2140 2571
Size: 2140 431
Bme1580I G_kGCm'C
Cuts at: 0 2339 2571
Size: 2339 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 635 2571
Size: 635 1936
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 2300 2571
Size: 573 1727 271
Fragments arranged by size:
1727 573 271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 2446 2571
Size: 1676 770 125
Fragments arranged by size:
1676 770 125
Bpu10I CC'TnA_GC
Cuts at: 0 338 2571
Size: 338 2233
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 1256 2571
Size: 457 799 1315
Fragments arranged by size:
1315 799 457
BsaAI yAC'GTr
Cuts at: 0 2332 2571
Size: 2332 239
BsaWI w'CCGG_w
Cuts at: 0 15 2530 2571
Size: 15 2515 41
Fragments arranged by size:
2515 41 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 304 322 325 328 331 334 337
Size: 70 234 18 3 3 3 3 3
Cuts at: 337 500 527 2571
Size: 163 27 2044
Fragments arranged by size:
2044 234 163 70 27 18 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 1419 2012 2571
Size: 1419 593 559
Fragments arranged by size:
1419 593 559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1116 1481 2571
Size: 1116 365 1090
Fragments arranged by size:
1116 1090 365
BsiHKAI G_wGCw'C
Cuts at: 0 444 1760 2432 2571
Size: 444 1316 672 139
Fragments arranged by size:
1316 672 444 139
Bsp1286I G_dGCh'C
Cuts at: 0 444 1626 1760 2339 2432 2571
Size: 444 1182 134 579 93 139
Fragments arranged by size:
1182 579 444 139 134 93
BspEI T'CCGG_A
Cuts at: 0 15 2571
Size: 15 2556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1138 2571
Size: 1138 1433
BsrDI GCAATG_nn'
Cuts at: 0 604 1619 1716 2571
Size: 604 1015 97 855
Fragments arranged by size:
1015 855 604 97
BsrGI T'GTAC_A
Cuts at: 0 1188 2292 2571
Size: 1188 1104 279
Fragments arranged by size:
1188 1104 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1486 1495 1750 2571
Size: 1486 9 255 821
Fragments arranged by size:
1486 821 255 9
BstBI TT'CG_AA
Cuts at: 0 62 2571
Size: 62 2509
BstZ17I GTA'TAC
Cuts at: 0 2555 2571
Size: 2555 16
Bsu36I CC'TnA_GG
Cuts at: 0 1317 2571
Size: 1317 1254
BtgI C'CryG_G
Cuts at: 0 809 974 2367 2571
Size: 809 165 1393 204
Fragments arranged by size:
1393 809 204 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 371 2053 2571
Size: 371 1682 518
Fragments arranged by size:
1682 518 371
BtsI GCAGTG_nn'
Cuts at: 0 347 2526 2571
Size: 347 2179 45
Fragments arranged by size:
2179 347 45
ClaI AT'CG_AT
Cuts at: 0 2316 2571
Size: 2316 255
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1361 1396 2571
Size: 1361 35 1175
Fragments arranged by size:
1361 1175 35
DraI TTT'AAA
Cuts at: 0 635 833 1310 1604 2571
Size: 635 198 477 294 967
Fragments arranged by size:
967 635 477 294 198
DrdI GACnn_nn'nnGTC
Cuts at: 0 202 2571
Size: 202 2369
EaeI y'GGCC_r
Cuts at: 0 806 2571
Size: 806 1765
EarI CTCTTCn'nnn_
Cuts at: 0 71 290 299 491 1097 2475 2571
Size: 71 219 9 192 606 1378 96
Fragments arranged by size:
1378 606 219 192 96 71 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 84 2571
Size: 84 2487
EcoICRI GAG'CTC
Cuts at: 0 442 2430 2571
Size: 442 1988 141
Fragments arranged by size:
1988 442 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 152 173 1067 1271 1304 1393 1531
Size: 91 61 21 894 204 33 89 138
Cuts at: 1531 1676 2446 2571
Size: 145 770 125
Fragments arranged by size:
894 770 204 145 138 125 91 89
61 33 21
EcoO109I rG'GnC_Cy
Cuts at: 0 963 2336 2421 2571
Size: 963 1373 85 150
Fragments arranged by size:
1373 963 150 85
EcoRV GAT'ATC
Cuts at: 0 1161 1640 2571
Size: 1161 479 931
Fragments arranged by size:
1161 931 479
FspI TGC'GCA
Cuts at: 0 629 2571
Size: 629 1942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 780 812 1684 1705 1716 1737 2571
Size: 780 32 872 21 11 21 834
Fragments arranged by size:
872 834 780 32 21 21 11
HincII GTy'rAC
Cuts at: 0 987 2571
Size: 987 1584
HindIII A'AGCT_T
Cuts at: 0 944 1031 1286 2571
Size: 944 87 255 1285
Fragments arranged by size:
1285 944 255 87
HpaI GTT'AAC
Cuts at: 0 987 2571
Size: 987 1584
MscI TGG'CCA
Cuts at: 0 808 2571
Size: 808 1763
MslI CAynn'nnrTG
Cuts at: 0 1382 2571
Size: 1382 1189
MspA1I CmG'CkG
Cuts at: 0 130 873 997 1419 2571
Size: 130 743 124 422 1152
Fragments arranged by size:
1152 743 422 130 124
NcoI C'CATG_G
Cuts at: 0 809 974 2571
Size: 809 165 1597
Fragments arranged by size:
1597 809 165
NdeI CA'TA_TG
Cuts at: 0 2249 2571
Size: 2249 322
NsiI A_TGCA'T
Cuts at: 0 2142 2571
Size: 2142 429
NspI r_CATG'y
Cuts at: 0 2167 2284 2571
Size: 2167 117 287
Fragments arranged by size:
2167 287 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 853 2571
Size: 853 1718
PspOMI G'GGCC_C
Cuts at: 0 2335 2571
Size: 2335 236
PspXI vC'TCGA_Gb
Cuts at: 0 482 2571
Size: 482 2089
PvuII CAG'CTG
Cuts at: 0 130 873 997 1419 2571
Size: 130 743 124 422 1152
Fragments arranged by size:
1152 743 422 130 124
SacI G_AGCT'C
Cuts at: 0 444 2432 2571
Size: 444 1988 139
Fragments arranged by size:
1988 444 139
SfcI C'TryA_G
Cuts at: 0 2217 2571
Size: 2217 354
SmlI C'TyrA_G
Cuts at: 0 482 2571
Size: 482 2089
SpeI A'CTAG_T
Cuts at: 0 1233 2571
Size: 1233 1338
SphI G_CATG'C
Cuts at: 0 2167 2571
Size: 2167 404
SspI AAT'ATT
Cuts at: 0 696 1748 1876 2571
Size: 696 1052 128 695
Fragments arranged by size:
1052 696 695 128
StuI AGG'CCT
Cuts at: 0 420 1315 2234 2571
Size: 420 895 919 337
Fragments arranged by size:
919 895 420 337
StyI C'CwwG_G
Cuts at: 0 585 809 974 2571
Size: 585 224 165 1597
Fragments arranged by size:
1597 585 224 165
SwaI ATTT'AAAT
Cuts at: 0 635 2571
Size: 635 1936
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 781 2571
Size: 448 333 1790
Fragments arranged by size:
1790 448 333
TatI w'GTAC_w
Cuts at: 0 1188 2292 2571
Size: 1188 1104 279
Fragments arranged by size:
1188 1104 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 2165 2197 2571
Size: 1771 32 362 32 374
Fragments arranged by size:
1771 374 362 32 32
XhoI C'TCGA_G
Cuts at: 0 482 2571
Size: 482 2089
XmnI GAAnn'nnTTC
Cuts at: 0 1289 2026 2571
Size: 1289 737 545
Fragments arranged by size:
1289 737 545
Enzymes that do cut:
AccI AclI AcuI AhdI AlfI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbsI BcgI BciVI BclI
BfrBI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BsrGI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRV FspI Hin4I HincII HindIII
HpaI MscI MslI MspA1I NcoI NdeI NsiI NspI
PflMI PspOMI PspXI PvuII SacI SfcI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BglI BglII BlpI BmgBI BmtI BpuEI BsaBI
BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrFI BssHII BssSI BstEII BstXI BstYI DraIII EagI
EcoNI EcoRI FalI FseI FspAI HaeII KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI Tth111I XbaI XcmI
XmaI ZraI