(Circular) (Six-base) MAPSORT of: pFN21AB9477.seq Check: 5916 from: 1 to: 5704 pFN21AB9477 5704 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2967 3514 278 Size: 53 83 186 936 1431 547 2468 Fragments arranged by size: 2468 1431 936 547 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2651 3767 5151 5419 1632 Size: 1019 1116 1384 268 1917 Fragments arranged by size: 1917 1384 1116 1019 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 4006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2560 3447 5019 2560 Size: 887 1572 3245 Fragments arranged by size: 3245 1572 887 ApaI G_GGCC'C Cuts at: 2132 2753 2132 Size: 621 5083 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2432 2610 2745 3029 1870 Size: 73 489 178 135 284 4545 Fragments arranged by size: 4545 489 284 178 135 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2818 2851 2818 Size: 33 5671 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2972 3025 4480 618 Size: 325 217 627 248 937 53 1455 1842 Fragments arranged by size: 1842 1455 937 627 325 248 217 53 BanII G_rGCy'C Cuts at: 1391 2132 2439 2753 3023 1391 Size: 741 307 314 270 4072 Fragments arranged by size: 4072 741 314 307 270 BbeI G_GCGC'C Cuts at: 2039 2976 2039 Size: 937 4767 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2482 2516 2638 2672 3895 Size: 34 50 34 461 34 122 34 1223 Cuts at: 3895 3929 1903 Size: 34 3678 Fragments arranged by size: 3678 1223 461 122 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 2664 1548 Size: 1116 4588 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2565 2694 4315 Size: 107 122 71 631 605 893 129 1621 Cuts at: 4315 136 Size: 1525 Fragments arranged by size: 1621 1525 893 631 605 129 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2064 3121 2064 Size: 1057 4647 Bme1580I G_kGCm'C Cuts at: 1790 2132 2717 2753 3765 4921 1790 Size: 342 585 36 1012 1156 2573 Fragments arranged by size: 2573 1156 1012 585 342 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 2720 3435 Size: 416 53 83 399 24 610 693 715 Cuts at: 3435 4393 442 Size: 958 1753 Fragments arranged by size: 1753 958 715 693 610 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2840 4364 1239 Size: 1142 459 1524 2579 Fragments arranged by size: 2579 1524 1142 459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 3829 4694 4992 2582 Size: 1247 865 298 3294 Fragments arranged by size: 3294 1247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2383 3495 493 Size: 711 1179 1112 2702 Fragments arranged by size: 2702 1179 1112 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2964 2973 2985 Size: 53 83 186 936 503 928 9 12 Cuts at: 2985 3511 3893 4528 275 Size: 526 382 635 1451 Fragments arranged by size: 1451 936 928 635 526 503 382 186 83 53 12 9 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 4818 BseYI C'CCAG_C Cuts at: 2011 2083 2158 2190 2563 2872 2926 4907 5403 Size: 72 75 32 373 309 54 1981 496 Cuts at: 5403 2011 Size: 2312 Fragments arranged by size: 2312 1981 496 373 309 75 72 54 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 1163 Size: 573 551 4580 Fragments arranged by size: 4580 573 551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3154 3915 4943 1540 Size: 275 186 997 156 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2439 2717 2753 3023 3765 3850 Size: 399 342 307 278 36 270 742 85 Cuts at: 3850 4921 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 307 278 270 85 36 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 3059 844 Size: 1979 236 3489 Fragments arranged by size: 3489 1979 236 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2571 4348 4535 1700 Size: 110 761 1777 187 2869 Fragments arranged by size: 2869 1777 761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 2177 3764 4776 2177 Size: 1587 1012 3105 Fragments arranged by size: 3105 1587 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2560 794 Size: 1766 3938 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2866 2011 Size: 855 4849 BstYI r'GATC_y Cuts at: 1972 2767 3034 3401 3786 3803 5255 5341 5353 Size: 795 267 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 795 385 367 346 267 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2074 2200 2757 2862 513 Size: 552 1009 126 557 105 3355 Fragments arranged by size: 3355 1009 557 552 126 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DraIII CAC_nnn'GTG Cuts at: 2323 2323 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2954 2990 4711 809 Size: 507 1638 36 1721 1802 Fragments arranged by size: 1802 1721 1638 507 36 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2686 2719 Size: 54 1425 50 275 18 385 471 33 Cuts at: 2719 2856 2904 2995 3151 4040 5669 8 Size: 137 48 91 156 889 1629 43 Fragments arranged by size: 1629 1425 889 471 385 275 156 137 91 54 50 48 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 3151 1537 Size: 275 1183 156 4090 Fragments arranged by size: 4090 1183 275 156 EarI CTCTTCn'nnn_ Cuts at: 1353 2781 3634 1353 Size: 1428 853 3423 Fragments arranged by size: 3423 1428 853 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2041 2127 2264 4291 4665 4811 Size: 1064 138 452 86 137 2027 374 146 Cuts at: 4811 387 Size: 1280 Fragments arranged by size: 2027 1280 1064 452 374 146 138 137 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2651 2840 3767 4364 5151 5419 Size: 393 749 270 189 927 597 787 268 Cuts at: 5419 1239 Size: 1524 Fragments arranged by size: 1524 927 787 749 597 393 270 268 189 EcoO109I rG'GnC_Cy Cuts at: 2102 2128 2129 2727 2749 2750 2911 2102 Size: 26 1 598 22 1 161 4895 Fragments arranged by size: 4895 598 161 26 22 1 1 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2039 2976 4851 1678 Size: 361 937 1875 2531 Fragments arranged by size: 2531 1875 937 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 4345 4377 4419 Size: 32 135 32 1187 32 1554 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 1187 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2035 2972 2035 Size: 937 4767 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 4818 5002 1305 Size: 1095 2418 184 2007 Fragments arranged by size: 2418 2007 1095 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3882 Fragments arranged by size: 3882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 2864 3662 4021 4180 518 Size: 648 1356 342 798 359 159 2042 Fragments arranged by size: 2042 1356 798 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2011 2194 2681 3120 3797 4945 5190 1606 Size: 405 183 487 439 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 487 439 405 245 183 NaeI GCC'GGC Cuts at: 2573 2573 Size: 5704 NarI GG'CG_CC Cuts at: 2036 2973 2036 Size: 937 4767 NcoI C'CATG_G Cuts at: 513 1065 2200 2862 513 Size: 552 1135 662 3355 Fragments arranged by size: 3355 1135 662 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2571 2571 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2075 2323 1108 Size: 967 248 4489 Fragments arranged by size: 4489 967 248 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PmlI CAC'GTG Cuts at: 2383 2383 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2314 3773 3805 5286 5318 2282 Size: 32 1459 32 1481 32 2668 Fragments arranged by size: 2668 1481 1459 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2727 2727 Size: 5704 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PspOMI G'GGCC_C Cuts at: 2128 2749 2128 Size: 621 5083 PstI C_TGCA'G Cuts at: 830 3056 830 Size: 2226 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2194 2194 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SanDI GG'GwC_CC Cuts at: 2727 2727 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 3052 4187 4868 5059 826 Size: 219 2007 1135 681 191 1471 Fragments arranged by size: 2007 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2037 2974 2037 Size: 937 4767 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 2747 3031 1872 Size: 875 284 4545 Fragments arranged by size: 4545 875 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3808 4709 4971 820 Size: 197 926 654 1211 901 262 1553 Fragments arranged by size: 1553 1211 926 901 654 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2270 2654 1517 Size: 753 384 4567 Fragments arranged by size: 4567 753 384 StyI C'CwwG_G Cuts at: 513 1065 2200 2862 2988 3143 513 Size: 552 1135 662 126 155 3074 Fragments arranged by size: 3074 1135 662 552 155 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2330 2918 3731 3748 3901 4086 Size: 1255 624 126 588 813 17 153 185 Cuts at: 4086 4531 325 Size: 445 1498 Fragments arranged by size: 1498 1255 813 624 588 445 185 153 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2198 2803 2869 2198 Size: 605 66 5033 Fragments arranged by size: 5033 605 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 2745 3029 1870 Size: 875 284 4545 Fragments arranged by size: 4545 875 284 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2965 3512 276 Size: 53 83 186 936 1431 547 2468 Fragments arranged by size: 2468 1431 936 547 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI