(Circular) (Six-base) MAPSORT of: pFN21AB9476.seq Check: 2730 from: 1 to: 5752 pFN21AB9476 5752 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2443 2443 Size: 5752 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 2403 3073 2403 Size: 670 5082 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2315 3815 5199 5467 1632 Size: 431 252 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 431 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 2666 1166 Size: 1500 4252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 2527 3495 5067 2527 Size: 968 1572 3212 Fragments arranged by size: 3212 1572 968 ApaI G_GGCC'C Cuts at: 2990 2990 Size: 5752 ApaLI G'TGCA_C Cuts at: 2648 2939 3809 4965 2648 Size: 291 870 1156 3435 Fragments arranged by size: 3435 1156 870 291 ApoI r'AATT_y Cuts at: 1493 2048 3061 3287 3439 5548 1493 Size: 555 1013 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2685 2694 3028 3077 1870 Size: 73 742 9 334 49 4545 Fragments arranged by size: 4545 742 334 73 49 9 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2403 2431 2689 3073 Size: 325 217 627 336 280 28 258 384 Cuts at: 3073 4528 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 384 336 325 280 258 217 28 BanII G_rGCy'C Cuts at: 1391 2367 2466 2661 2990 3071 1391 Size: 976 99 195 329 81 4072 Fragments arranged by size: 4072 976 329 195 99 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 4182 Fragments arranged by size: 4182 971 599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 2567 2994 Size: 107 122 71 631 605 646 249 427 Cuts at: 2994 4363 136 Size: 1369 1525 Fragments arranged by size: 1525 1369 646 631 605 427 249 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2572 2652 2694 2943 2990 3813 4969 1790 Size: 782 80 42 249 47 823 1156 2573 Fragments arranged by size: 2573 1156 823 782 249 80 47 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2558 2806 2891 4412 1239 Size: 1211 108 248 85 1521 2579 Fragments arranged by size: 2579 1521 1211 248 108 85 Bpu10I CC'TnA_GC Cuts at: 2238 2238 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 2774 3877 4742 5040 2567 Size: 207 1103 865 298 3279 Fragments arranged by size: 3279 1103 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 3559 3941 4576 Size: 53 83 186 936 657 1369 382 635 Cuts at: 4576 275 Size: 1451 Fragments arranged by size: 1451 1369 936 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5527 Fragments arranged by size: 5527 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2831 1186 Size: 1645 4107 BseYI C'CCAG_C Cuts at: 2119 2148 2193 2399 2562 2673 4955 5451 2119 Size: 29 45 206 163 111 2282 496 2420 Fragments arranged by size: 2420 2282 496 206 163 111 45 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2815 1163 Size: 573 1079 4100 Fragments arranged by size: 4100 1079 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 2466 2652 2943 3813 3898 4969 2367 Size: 99 186 291 870 85 1071 3150 Fragments arranged by size: 3150 1071 870 291 186 99 85 BsmI GAATG_Cn' Cuts at: 2239 2817 3263 3356 2239 Size: 578 446 93 4635 Fragments arranged by size: 4635 578 446 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2466 2572 2652 2661 2694 2943 Size: 399 577 99 106 80 9 33 249 Cuts at: 2943 2990 3071 3813 3898 4969 1391 Size: 47 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 399 249 106 99 85 81 80 47 33 9 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2443 3107 844 Size: 1373 226 664 3489 Fragments arranged by size: 3489 1373 664 226 BsrBI CCG'CTC Cuts at: 1394 1823 2629 3639 1394 Size: 429 806 1010 3507 Fragments arranged by size: 3507 1010 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 2114 3812 4824 2114 Size: 1698 1012 3042 Fragments arranged by size: 3042 1698 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2116 794 Size: 1322 4430 BstBI TT'CG_AA Cuts at: 2247 4556 2247 Size: 2309 3443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2644 2668 2644 Size: 24 5728 BstYI r'GATC_y Cuts at: 1972 2472 3082 3449 3834 3851 5303 5389 5401 Size: 500 610 367 385 17 1452 86 12 Cuts at: 5401 5747 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 610 500 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2556 2556 Size: 5752 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2661 2850 3022 513 Size: 552 939 27 630 189 172 3243 Fragments arranged by size: 3243 939 630 552 189 172 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2913 487 Size: 683 56 1687 3326 Fragments arranged by size: 3326 1687 683 56 BtsI GCAGTG_nn' Cuts at: 2180 3268 4062 4082 2180 Size: 1088 794 20 3850 Fragments arranged by size: 3850 1088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2310 2347 3199 Size: 54 1425 50 275 18 480 37 852 Cuts at: 3199 4088 5717 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 852 480 275 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2261 2372 3682 1353 Size: 678 230 111 1310 3423 Fragments arranged by size: 3423 1310 678 230 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2365 2464 2365 Size: 99 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2315 2450 2558 2806 2891 3815 Size: 393 431 252 135 108 248 85 924 Cuts at: 3815 4412 5199 5467 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 431 393 268 252 248 135 108 85 EcoO109I rG'GnC_Cy Cuts at: 2533 2986 2533 Size: 453 5299 EcoRI G'AATT_C Cuts at: 2048 3061 3439 2048 Size: 1013 378 4361 Fragments arranged by size: 4361 1013 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2986 2068 Size: 918 4834 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 3016 4899 1678 Size: 1338 1883 2531 Fragments arranged by size: 2531 1883 1338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 4393 4425 4467 Size: 32 135 32 803 32 1986 32 42 Cuts at: 4467 4499 1373 Size: 32 2626 Fragments arranged by size: 2626 1986 803 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2599 3096 3342 669 Size: 988 281 661 497 246 3079 Fragments arranged by size: 3079 988 661 497 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 2407 3077 2407 Size: 670 5082 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 10 Size: 54 1425 343 517 3413 Fragments arranged by size: 3413 1425 517 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 2666 2736 3710 4069 4228 518 Size: 648 1443 57 70 974 359 159 2042 Fragments arranged by size: 2042 1443 974 648 359 159 70 57 MspA1I CmG'CkG Cuts at: 1606 2148 2443 2566 2677 2826 3024 3168 3845 Size: 542 295 123 111 149 198 144 677 Cuts at: 3845 4993 5238 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 542 295 245 198 149 144 123 111 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4261 Fragments arranged by size: 4261 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 2990 1348 Size: 1642 4110 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PspOMI G'GGCC_C Cuts at: 2986 2986 Size: 5752 PstI C_TGCA'G Cuts at: 830 2532 2553 3104 830 Size: 1702 21 551 3478 Fragments arranged by size: 3478 1702 551 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2677 2826 2677 Size: 149 5603 SacI G_AGCT'C Cuts at: 2367 2466 2367 Size: 99 5653 SacII CC_GC'GG Cuts at: 3025 3025 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2031 2261 2372 2031 Size: 230 111 5411 Fragments arranged by size: 5411 230 111 SbfI CC_TGCA'GG Cuts at: 2532 3104 2532 Size: 572 5180 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2528 2549 2760 3100 4235 4916 5107 Size: 219 1483 21 211 340 1135 681 191 Cuts at: 5107 826 Size: 1471 Fragments arranged by size: 1483 1471 1135 681 340 219 211 191 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2318 2318 Size: 5752 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 2687 3030 3079 1872 Size: 815 343 49 4545 Fragments arranged by size: 4545 815 343 49 SmlI C'TyrA_G Cuts at: 820 1017 1943 2582 2753 3856 4757 5019 820 Size: 197 926 639 171 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 639 262 197 171 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2969 1517 Size: 1452 4300 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 2982 3191 513 Size: 552 939 54 924 209 3074 Fragments arranged by size: 3074 939 924 552 209 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2750 3779 3796 3949 4134 4579 Size: 1255 531 639 1029 17 153 185 445 Cuts at: 4579 325 Size: 1498 Fragments arranged by size: 1498 1255 1029 639 531 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3998 5710 96 Size: 274 80 33 51 1443 2021 1712 138 Fragments arranged by size: 2021 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 5712 5744 2405 Size: 32 3275 32 2413 Fragments arranged by size: 3275 2413 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2145 2145 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 2685 3028 3077 1870 Size: 815 343 49 4545 Fragments arranged by size: 4545 815 343 49 XmnI GAAnn'nnTTC Cuts at: 2048 3881 2048 Size: 1833 3919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI