Restriction map for
pFN21AB9476
(Linear) (Six-base) MAPSORT of: KIBB9476.seq Check: 2372 from: 1 to: 1047
KIBB9476.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 438 1047
Size: 438 609
Acc65I G'GTAC_C
Cuts at: 0 398 1047
Size: 398 649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 310 1047
Size: 58 252 737
Fragments arranged by size:
737 252 58
AleI CACnn'nnGTG
Cuts at: 0 661 1047
Size: 661 386
AlwNI CAG_nnn'CTG
Cuts at: 0 522 1047
Size: 522 525
ApaI G_GGCC'C
Cuts at: 0 985 1047
Size: 985 62
ApaLI G'TGCA_C
Cuts at: 0 643 934 1047
Size: 643 291 113
Fragments arranged by size:
643 291 113
ApoI r'AATT_y
Cuts at: 0 43 1047
Size: 43 1004
AvaI C'yCGr_G
Cuts at: 0 680 689 1023 1047
Size: 680 9 334 24
Fragments arranged by size:
680 334 24 9
BanI G'GyrC_C
Cuts at: 0 118 398 426 684 1047
Size: 118 280 28 258 363
Fragments arranged by size:
363 280 258 118 28
BanII G_rGCy'C
Cuts at: 0 362 461 656 985 1047
Size: 362 99 195 329 62
Fragments arranged by size:
362 329 195 99 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 1047
Size: 493 554
BglI GCCn_nnn'nGGC
Cuts at: 0 313 562 989 1047
Size: 313 249 427 58
Fragments arranged by size:
427 313 249 58
Bme1580I G_kGCm'C
Cuts at: 0 567 647 689 938 985 1047
Size: 567 80 42 249 47 62
Fragments arranged by size:
567 249 80 62 47 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 553 801 886 1047
Size: 445 108 248 85 161
Fragments arranged by size:
445 248 161 108 85
Bpu10I CC'TnA_GC
Cuts at: 0 233 1047
Size: 233 814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 769 1047
Size: 562 207 278
Fragments arranged by size:
562 278 207
BsaHI Gr'CG_yC
Cuts at: 0 185 1047
Size: 185 862
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 826 1047
Size: 826 221
BseYI C'CCAG_C
Cuts at: 0 114 143 188 394 557 668 1047
Size: 114 29 45 206 163 111 379
Fragments arranged by size:
379 206 163 114 111 45 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1047
Size: 810 237
BsiHKAI G_wGCw'C
Cuts at: 0 362 461 647 938 1047
Size: 362 99 186 291 109
Fragments arranged by size:
362 291 186 109 99
BsmI GAATG_Cn'
Cuts at: 0 234 812 1047
Size: 234 578 235
Fragments arranged by size:
578 235 234
Bsp1286I G_dGCh'C
Cuts at: 0 362 461 567 647 656 689 938 985
Size: 362 99 106 80 9 33 249 47
Cuts at: 985 1047
Size: 62
Fragments arranged by size:
362 249 106 99 80 62 47 33
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 438 1047
Size: 212 226 609
Fragments arranged by size:
609 226 212
BsrBI CCG'CTC
Cuts at: 0 624 1047
Size: 624 423
BssSI C'ACGA_G
Cuts at: 0 109 1047
Size: 109 938
BstAPI GCAn_nnn'nTGC
Cuts at: 0 111 1047
Size: 111 936
BstBI TT'CG_AA
Cuts at: 0 242 1047
Size: 242 805
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 663 1047
Size: 639 24 384
Fragments arranged by size:
639 384 24
BstYI r'GATC_y
Cuts at: 0 467 1047
Size: 467 580
Bsu36I CC'TnA_GG
Cuts at: 0 551 1047
Size: 551 496
BtgI C'CryG_G
Cuts at: 0 26 656 845 1017 1047
Size: 26 630 189 172 30
Fragments arranged by size:
630 189 172 30 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 908 1047
Size: 908 139
BtsI GCAGTG_nn'
Cuts at: 0 175 1047
Size: 175 872
EaeI y'GGCC_r
Cuts at: 0 305 342 1047
Size: 305 37 705
Fragments arranged by size:
705 305 37
EarI CTCTTCn'nnn_
Cuts at: 0 26 256 367 1047
Size: 26 230 111 680
Fragments arranged by size:
680 230 111 26
EcoICRI GAG'CTC
Cuts at: 0 360 459 1047
Size: 360 99 588
Fragments arranged by size:
588 360 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 310 445 553 801 886 1047
Size: 58 252 135 108 248 85 161
Fragments arranged by size:
252 248 161 135 108 85 58
EcoO109I rG'GnC_Cy
Cuts at: 0 528 981 1047
Size: 528 453 66
Fragments arranged by size:
528 453 66
EcoRI G'AATT_C
Cuts at: 0 43 1047
Size: 43 1004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 981 1047
Size: 63 918 66
Fragments arranged by size:
918 66 63
HaeII r_GCGC'y
Cuts at: 0 1011 1047
Size: 1011 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 1047
Size: 370 32 645
Fragments arranged by size:
645 370 32
HincII GTy'rAC
Cuts at: 0 594 1047
Size: 594 453
KpnI G_GTAC'C
Cuts at: 0 402 1047
Size: 402 645
MscI TGG'CCA
Cuts at: 0 344 1047
Size: 344 703
MslI CAynn'nnrTG
Cuts at: 0 604 661 731 1047
Size: 604 57 70 316
Fragments arranged by size:
604 316 70 57
MspA1I CmG'CkG
Cuts at: 0 143 438 561 672 821 1019 1047
Size: 143 295 123 111 149 198 28
Fragments arranged by size:
295 198 149 143 123 111 28
PasI CC'CwG_GG
Cuts at: 0 985 1047
Size: 985 62
PspOMI G'GGCC_C
Cuts at: 0 981 1047
Size: 981 66
PstI C_TGCA'G
Cuts at: 0 527 548 1047
Size: 527 21 499
Fragments arranged by size:
527 499 21
PvuII CAG'CTG
Cuts at: 0 672 821 1047
Size: 672 149 226
Fragments arranged by size:
672 226 149
SacI G_AGCT'C
Cuts at: 0 362 461 1047
Size: 362 99 586
Fragments arranged by size:
586 362 99
SacII CC_GC'GG
Cuts at: 0 1020 1047
Size: 1020 27
SapI GCTCTTCn'nnn_
Cuts at: 0 26 256 367 1047
Size: 26 230 111 680
Fragments arranged by size:
680 230 111 26
SbfI CC_TGCA'GG
Cuts at: 0 527 1047
Size: 527 520
SfcI C'TryA_G
Cuts at: 0 523 544 755 1047
Size: 523 21 211 292
Fragments arranged by size:
523 292 211 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 313 1047
Size: 313 734
SmaI CCC'GGG
Cuts at: 0 682 1025 1047
Size: 682 343 22
Fragments arranged by size:
682 343 22
SmlI C'TyrA_G
Cuts at: 0 577 748 1047
Size: 577 171 299
Fragments arranged by size:
577 299 171
StuI AGG'CCT
Cuts at: 0 964 1047
Size: 964 83
StyI C'CwwG_G
Cuts at: 0 53 977 1047
Size: 53 924 70
Fragments arranged by size:
924 70 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 745 1047
Size: 106 639 302
Fragments arranged by size:
639 302 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1047
Size: 400 32 615
Fragments arranged by size:
615 400 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 140 1047
Size: 140 907
XmaI C'CCGG_G
Cuts at: 0 680 1023 1047
Size: 680 343 24
Fragments arranged by size:
680 343 24
XmnI GAAnn'nnTTC
Cuts at: 0 43 1047
Size: 43 1004
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BglI Bme1580I BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MscI MslI
MspA1I PasI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SfiI SmaI SmlI StuI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI