Restriction map for
pFN21AB9475
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 1024 AAATTC [GA]^AATT[CT]
AflIII 880 ACACGT A^C[GA][CT]GT
AhdI 790 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1024 AAATTC [GA]^AATT[CT]
AspEI 790 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 769 GAGCAC G[AT]GC[AT]^C
BalI 28 TGGCCA TGG^CCA
BanI 822 GGTGCC G^G[CT][GA]CC
BanII 78 GGGCTC G[GA]GC[CT]^C
BciVI 397 GTATCC GTATCC(6/5)
BfaI 553 CTAG C^TAG
BglII 15 AGATCT A^GATCT
BsaI 229 GAGACC GGTCTC(1/5)
BsiHKAI 769 GAGCAC G[AT]GC[AT]^C
BsiYI 826 CCTTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 826 CCTTCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 138 ACCTGC ACCTGC(4/8)
BsrDI 461 GCAATG GCAATG(2/0)
BstYI 15 AGATCT [GA]^GATC[CT]
DpnII 16 GATC ^GATC
DraI 955 TTTAAA TTT^AAA
EaeI 28 TGGCCA [CT]^GGCC[GA]
Eam1105I 790 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 826 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 292 GATATC GAT^ATC
EcoT14I 96 CCAAGG C^C[AT][AT]GG
FauI 405 CCCGC CCCGC(4/6)
HindIII 177 AAGCTT A^AGCTT
HphI 829 TCACC GGTGA(8/7)
MaeI 553 CTAG C^TAG
MaeII 882 ACGT A^CGT
MboI 16 GATC ^GATC
MflI 15 AGATCT [GA]^GATC[CT]
MluNI 28 TGGCCA TGG^CCA
MscI 28 TGGCCA TGG^CCA
MspA1I 536 CAGCTG C[AC]G^C[GT]G
MwoI 696 GCAGCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 16 GATC ^GATC
PshAI 628 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 536 CAGCTG CAG^CTG
SapI 665 GAAGAGC GCTCTTC(1/4)
Sau3AI 16 GATC ^GATC
SexAI 392 ACCAGGT A^CC[AT]GGT
StyI 96 CCAAGG C^C[AT][AT]GG
TaqI 20 TCGA T^CGA
TfiI 898 GAATC G^A[AT]TC
Tsp509I 1025 AATT ^AATT
TthHB8I 20 TCGA T^CGA
XbaI 552 TCTAGA T^CTAGA
XhoII 15 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 7 CCGC CCGC(-3/-1)
406 CCGC
AfaI 136 GTAC GT^AC
456 GTAC
AlwNI 70 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
700 CAGAGGCTG
BmyI 78 GGGCTC G[AGT]GC[ACT]^C
769 GAGCAC
BpmI 278 CTCCAG CTGGAG(16/14)
637 CTCCAG
Bsp1286I 78 GGGCTC G[AGT]GC[ACT]^C
769 GAGCAC
DraIII 146 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
881 CACGTCGTG
MseI 892 TTAA T^TAA
956 TTAA
NspI 447 ACATGC [GA]CATG^[CT]
670 GCATGT
PstI 203 CTGCAG CTGCA^G
852 CTGCAG
RsaI 136 GTAC GT^AC
456 GTAC
SfcI 203 CTGCAG C^T[GA][CT]AG
852 CTGCAG
Tru9I 892 TTAA T^TAA
956 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AvaII BbsI BbvI BpuAI
BsaJI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeIII MboII
MnlI MvaI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI