(Circular) (Six-base) MAPSORT of: pFN21AB9475.seq Check: 792 from: 1 to: 5734 pFN21AB9475 5734 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2145 2679 2732 2769 2790 2806 3797 Size: 479 34 534 53 37 21 16 991 Cuts at: 3797 5181 5449 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 991 534 479 268 53 37 34 21 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2885 4633 5610 1203 Size: 1682 1748 977 1327 Fragments arranged by size: 1748 1682 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 2800 4463 2800 Size: 1663 4071 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2885 1184 Size: 1701 4033 AlwNI CAG_nnn'CTG Cuts at: 2080 2710 3477 5049 2080 Size: 630 767 1572 2765 Fragments arranged by size: 2765 1572 767 630 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3029 3043 3269 3421 5530 1493 Size: 1536 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1536 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 3059 1870 Size: 73 1116 4545 Fragments arranged by size: 4545 1116 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2827 3055 4510 618 Size: 325 217 627 1040 228 1455 1842 Fragments arranged by size: 1842 1455 1040 627 325 228 217 BanII G_rGCy'C Cuts at: 1391 2087 3053 1391 Size: 696 966 4072 Fragments arranged by size: 4072 966 696 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 2088 2796 928 Size: 971 140 49 708 3866 Fragments arranged by size: 3866 971 708 140 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3925 3959 1903 Size: 34 50 34 1904 34 3678 Fragments arranged by size: 3678 1904 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2413 3626 4836 2413 Size: 1213 1210 3311 Fragments arranged by size: 3311 1213 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2020 5729 2020 Size: 3709 2025 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 3795 4951 1790 Size: 2005 1156 2573 Fragments arranged by size: 2573 2005 1156 BmgBI CAC'GTC Cuts at: 1318 2888 1318 Size: 1570 4164 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 3465 4423 Size: 416 53 83 399 24 629 1419 958 Cuts at: 4423 442 Size: 1753 Fragments arranged by size: 1753 1419 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 2627 4394 1239 Size: 1029 359 1767 2579 Fragments arranged by size: 2579 1767 1029 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2228 4397 882 Size: 617 729 2169 2219 Fragments arranged by size: 2219 2169 729 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 1178 Size: 30 165 30 1278 30 4201 Fragments arranged by size: 4201 1278 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5734 BseYI C'CCAG_C Cuts at: 2238 4937 5433 2238 Size: 2699 496 2539 Fragments arranged by size: 2699 2539 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5161 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2778 3795 3880 4951 2778 Size: 1017 85 1071 3561 Fragments arranged by size: 3561 1071 1017 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2778 3053 3795 3880 4951 1391 Size: 399 297 691 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 297 275 85 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 3089 844 Size: 1308 937 3489 Fragments arranged by size: 3489 1308 937 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2473 4229 4403 65 Size: 1183 1225 1756 174 1396 Fragments arranged by size: 1756 1396 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2020 3064 3431 3816 3833 5285 5371 5383 Size: 48 1044 367 385 17 1452 86 12 Cuts at: 5383 5729 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1044 385 367 346 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 3250 4044 4064 3250 Size: 794 20 4920 Fragments arranged by size: 4920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2425 2460 529 Size: 35 1861 35 3803 Fragments arranged by size: 3803 1861 35 35 DraI TTT'AAA Cuts at: 2962 3038 2962 Size: 76 5658 DraIII CAC_nnn'GTG Cuts at: 2156 2891 2156 Size: 735 4999 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 3181 4070 Size: 54 1425 50 275 18 203 1148 889 Cuts at: 4070 5699 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1148 889 275 203 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2665 2841 3664 1353 Size: 768 544 176 823 3423 Fragments arranged by size: 3423 823 768 544 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2145 2268 2627 2679 2732 2769 Size: 393 479 34 123 359 52 53 37 Cuts at: 2769 2790 2806 3797 4394 5181 5449 1239 Size: 21 16 991 597 787 268 1524 Fragments arranged by size: 1524 991 787 597 479 393 359 268 123 53 52 37 34 21 16 EcoNI CCTnn'n_nnAGG Cuts at: 2835 2835 Size: 5734 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 EcoRV GAT'ATC Cuts at: 2299 2299 Size: 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 2200 Size: 5734 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 4375 4407 4449 Size: 32 135 32 1214 32 1557 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1557 1214 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 2182 748 Size: 1434 4300 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2972 4848 5032 1305 Size: 1667 1876 184 2007 Fragments arranged by size: 2007 1876 1667 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2035 10 Size: 54 1425 343 203 3709 Fragments arranged by size: 3709 1425 343 203 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 3150 3827 4975 5220 1606 Size: 937 607 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 607 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2456 2679 3092 4637 1132 Size: 97 1227 223 413 1545 2229 Fragments arranged by size: 2229 1545 1227 413 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2637 1613 Size: 1024 4710 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PstI C_TGCA'G Cuts at: 830 2212 2861 3086 830 Size: 1382 649 225 3478 Fragments arranged by size: 3478 1382 649 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2543 2543 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SapI GCTCTTCn'nnn_ Cuts at: 2665 2665 Size: 5734 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SexAI A'CCwGG_T Cuts at: 2397 2397 Size: 5734 SfcI C'TryA_G Cuts at: 826 1045 2208 2857 3082 4217 4898 5089 826 Size: 219 1163 649 225 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 681 649 225 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2101 3173 513 Size: 552 1036 1072 3074 Fragments arranged by size: 3074 1072 1036 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 3761 3778 3931 4116 4561 325 Size: 1255 869 1312 17 153 185 445 1498 Fragments arranged by size: 1498 1312 1255 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 3980 5692 Size: 274 80 33 51 1443 483 1520 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1520 1443 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 2681 2713 5694 5726 2212 Size: 32 437 32 2981 32 2220 Fragments arranged by size: 2981 2220 437 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 2557 3070 2557 Size: 513 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI