(Circular) (Six-base) MAPSORT of: pFN21AB9472.seq Check: 9975 from: 1 to: 6217 pFN21AB9472 6217 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2110 2257 2672 2110 Size: 147 415 5655 Fragments arranged by size: 5655 415 147 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2648 2867 4280 5664 5932 1632 Size: 644 372 219 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 644 372 268 219 AfeI AGC'GCT Cuts at: 3322 3322 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 2295 2934 3475 5116 6093 1203 Size: 1092 639 541 1641 977 1327 Fragments arranged by size: 1641 1327 1092 977 639 541 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 2938 4946 2938 Size: 2008 4209 AleI CACnn'nnGTG Cuts at: 1166 2774 1166 Size: 1608 4609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2223 2254 2845 3401 3960 5532 2223 Size: 31 591 556 559 1572 2908 Fragments arranged by size: 2908 1572 591 559 556 31 ApaI G_GGCC'C Cuts at: 2391 3389 2391 Size: 998 5219 ApaLI G'TGCA_C Cuts at: 3220 4274 5430 3220 Size: 1054 1156 4007 Fragments arranged by size: 4007 1156 1054 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2079 3542 1870 Size: 73 136 1463 4545 Fragments arranged by size: 4545 1463 136 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2472 2532 2687 2864 3023 Size: 325 217 627 685 60 155 177 159 Cuts at: 3023 3179 3252 3538 4993 618 Size: 156 73 286 1455 1842 Fragments arranged by size: 1842 1455 685 627 325 286 217 177 159 156 155 73 60 BanII G_rGCy'C Cuts at: 1391 2018 2391 2601 3389 3536 1391 Size: 627 373 210 788 147 4072 Fragments arranged by size: 4072 788 627 373 210 147 BbeI G_GCGC'C Cuts at: 2476 2691 2868 3027 2476 Size: 215 177 159 5666 Fragments arranged by size: 5666 215 177 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3160 928 Size: 971 1261 3985 Fragments arranged by size: 3985 1261 971 BbvCI CC'TCA_GC Cuts at: 3317 3317 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2251 4828 136 Size: 107 122 71 631 605 579 2577 1525 Fragments arranged by size: 2577 1525 631 605 579 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2595 3634 2595 Size: 1039 5178 Bme1580I G_kGCm'C Cuts at: 1790 2159 2391 2440 2535 2989 3182 3224 3255 Size: 369 232 49 95 454 193 42 31 Cuts at: 3255 3389 4278 5434 1790 Size: 134 889 1156 2573 Fragments arranged by size: 2573 1156 889 454 369 232 193 134 95 49 42 31 BmgBI CAC'GTC Cuts at: 1318 2796 2824 2935 1318 Size: 1478 28 111 4600 Fragments arranged by size: 4600 1478 111 28 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2153 3389 3392 Size: 416 53 83 399 24 736 1236 3 Cuts at: 3392 3948 4906 442 Size: 556 958 1753 Fragments arranged by size: 1753 1236 958 736 556 416 399 83 53 24 3 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2245 4877 1239 Size: 1006 2632 2579 Fragments arranged by size: 2632 2579 1006 Bpu10I CC'TnA_GC Cuts at: 2327 3317 2327 Size: 990 5227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 4342 5207 5505 2498 Size: 1844 865 298 3210 Fragments arranged by size: 3210 1844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3363 4880 882 Size: 617 1864 1517 2219 Fragments arranged by size: 2219 1864 1517 617 BsaAI yAC'GTr Cuts at: 493 1204 2296 2553 3478 4008 493 Size: 711 1092 257 925 530 2702 Fragments arranged by size: 2702 1092 925 711 530 257 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2473 2688 2865 Size: 53 83 186 936 476 464 215 177 Cuts at: 2865 3024 4024 4406 5041 275 Size: 159 1000 382 635 1451 Fragments arranged by size: 1451 1000 936 635 476 464 382 215 186 177 159 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3341 3371 1178 Size: 30 165 30 1938 30 4024 Fragments arranged by size: 4024 1938 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2389 3097 3362 1186 Size: 1203 708 265 4041 Fragments arranged by size: 4041 1203 708 265 BseYI C'CCAG_C Cuts at: 2050 2131 3154 3372 5420 5916 2050 Size: 81 1023 218 2048 496 2351 Fragments arranged by size: 2351 2048 1023 496 218 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 2444 2642 3280 3667 4428 Size: 275 186 69 374 198 638 387 761 Cuts at: 4428 5456 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 638 387 374 275 198 186 69 BsiHKAI G_wGCw'C Cuts at: 2748 3224 4278 4363 5434 2748 Size: 476 1054 85 1071 3531 Fragments arranged by size: 3531 1071 1054 476 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2159 2391 2440 2535 2601 2748 Size: 399 228 141 232 49 95 66 147 Cuts at: 2748 2989 3182 3224 3255 3389 3536 4278 4363 Size: 241 193 42 31 134 147 742 85 Cuts at: 4363 5434 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 241 232 228 193 147 147 141 134 95 85 66 49 42 31 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2110 2257 2643 2672 3572 844 Size: 1266 147 386 29 900 3489 Fragments arranged by size: 3489 1266 900 386 147 29 BsrBI CCG'CTC Cuts at: 1394 1823 2275 4104 1394 Size: 429 452 1829 3507 Fragments arranged by size: 3507 1829 452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2285 2830 3158 4861 5048 1700 Size: 110 255 220 545 328 1703 187 2869 Fragments arranged by size: 2869 1703 545 328 255 220 187 110 BsrGI T'GTAC_A Cuts at: 96 3009 96 Size: 2913 3304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2896 3034 2896 Size: 138 6079 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2114 2493 2925 3033 3131 3138 513 Size: 552 1049 379 432 108 98 7 3592 Fragments arranged by size: 3592 1049 552 432 379 108 98 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2159 2438 3733 4527 4547 2159 Size: 279 1295 794 20 3829 Fragments arranged by size: 3829 1295 794 279 20 ClaI AT'CG_AT Cuts at: 1554 2892 1554 Size: 1338 4879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DraIII CAC_nnn'GTG Cuts at: 2764 2764 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2108 5224 809 Size: 507 792 3116 1802 Fragments arranged by size: 3116 1802 792 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2203 2523 Size: 54 1425 50 275 18 237 136 320 Cuts at: 2523 2639 2902 3117 3309 3664 4553 6182 8 Size: 116 263 215 192 355 889 1629 43 Fragments arranged by size: 1629 1425 889 355 320 275 263 237 215 192 136 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 2639 3664 1537 Size: 275 255 572 1025 4090 Fragments arranged by size: 4090 1025 572 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 4147 1353 Size: 2794 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 4804 5178 5324 387 Size: 1064 138 525 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 525 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2245 2276 2648 2867 4280 4877 5664 Size: 393 613 31 372 219 1413 597 787 Cuts at: 5664 5932 1239 Size: 268 1524 Fragments arranged by size: 1524 1413 787 613 597 393 372 268 219 31 EcoO109I rG'GnC_Cy Cuts at: 2055 2084 2170 2387 2416 3295 2055 Size: 29 86 217 29 879 4977 Fragments arranged by size: 4977 879 217 86 29 29 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 2816 4723 2816 Size: 1907 4310 HaeII r_GCGC'y Cuts at: 1678 2476 2691 2868 3027 3057 3165 3324 5364 Size: 798 215 177 159 30 108 159 2040 Cuts at: 5364 1678 Size: 2531 Fragments arranged by size: 2531 2040 798 215 177 159 159 108 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2924 2956 3287 Size: 32 135 32 812 32 508 32 331 Cuts at: 3287 3319 3341 3373 4858 4890 4932 4964 1373 Size: 32 22 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 812 508 331 135 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3561 3807 669 Size: 988 281 397 1226 246 3079 Fragments arranged by size: 3079 1226 988 397 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2472 2687 2864 3023 2472 Size: 215 177 159 5666 Fragments arranged by size: 5666 215 177 159 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2525 10 Size: 54 1425 343 693 3702 Fragments arranged by size: 3702 1425 693 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2774 2894 4175 4534 4693 518 Size: 648 1389 219 120 1281 359 159 2042 Fragments arranged by size: 2042 1389 1281 648 359 219 159 120 MspA1I CmG'CkG Cuts at: 1606 2044 2182 2220 2529 2594 2652 2725 3285 Size: 438 138 38 309 65 58 73 560 Cuts at: 3285 3633 4310 5458 5703 1606 Size: 348 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 560 438 348 309 245 138 73 65 58 38 NaeI GCC'GGC Cuts at: 2287 3160 2287 Size: 873 5344 NarI GG'CG_CC Cuts at: 2473 2688 2865 3024 2473 Size: 215 177 159 5666 Fragments arranged by size: 5666 215 177 159 NcoI C'CATG_G Cuts at: 513 1065 3033 513 Size: 552 1968 3697 Fragments arranged by size: 3697 1968 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2285 3158 2285 Size: 873 5344 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3010 3575 5120 1132 Size: 97 1781 565 1545 2229 Fragments arranged by size: 2229 1781 1545 565 97 PasI CC'CwG_GG Cuts at: 1348 2369 1348 Size: 1021 5196 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 3401 1108 Size: 2293 3924 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 2553 3478 2553 Size: 925 5292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2416 3295 2170 Size: 246 879 5092 Fragments arranged by size: 5092 879 246 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2387 3385 2387 Size: 998 5219 PstI C_TGCA'G Cuts at: 830 2154 3569 830 Size: 1324 1415 3478 Fragments arranged by size: 3478 1415 1324 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 2170 2170 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2150 3040 3565 4700 5381 5572 826 Size: 219 1105 890 525 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 890 681 525 219 191 SfoI GGC'GCC Cuts at: 2474 2689 2866 3025 2474 Size: 215 177 159 5666 Fragments arranged by size: 5666 215 177 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 4321 5222 5484 820 Size: 197 926 570 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2573 1517 Size: 1056 5161 StyI C'CwwG_G Cuts at: 513 1065 2196 3019 3033 3656 513 Size: 552 1131 823 14 623 3074 Fragments arranged by size: 3074 1131 823 623 552 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 4463 6175 Size: 274 80 33 51 1443 1032 1454 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1712 1454 1443 1032 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 2794 1316 Size: 1478 4739 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 2893 2707 Size: 186 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI