Restriction map for
pFN21AB9471
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AvrII BamHI
BbrPI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1566 GGTACC G^GTACC
AccI 1867 GTCTAC GT^[AC][GT]AC
AccII 1361 CGCG CG^CG
AcsI 594 GAATTC [GA]^AATT[CT]
AcyI 1370 GGCGCC G[GA]^CG[CT]C
ApoI 594 GAATTC [GA]^AATT[CT]
Asp718I 1566 GGTACC G^GTACC
BalI 2994 TGGCCA TGG^CCA
BbeI 1370 GGCGCC GGCGC^C
BcgI 1847 GCACTTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1675 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1675 GCTGAGC GC^T[ACGT]AGC
BsaHI 1370 GGCGCC G[GA]^CG[CT]C
BsiEI 2640 CGGCCG CG[GA][CT]^CG
BstUI 1361 CGCG CG^CG
CelII 1675 GCTGAGC GC^T[ACGT]AGC
DraIII 667 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2640 CGGCCG C^GGCCG
EclXI 2640 CGGCCG C^GGCCG
Eco52I 2640 CGGCCG C^GGCCG
EcoRI 594 GAATTC G^AATTC
Hin1I 1370 GGCGCC G[GA]^CG[CT]C
KasI 1370 GGCGCC G^GCGCC
KpnI 1566 GGTACC GGTAC^C
MaeII 2171 ACGT A^CGT
MluNI 2994 TGGCCA TGG^CCA
MscI 2994 TGGCCA TGG^CCA
MseI 2720 TTAA T^TAA
MvnI 1361 CGCG CG^CG
NarI 1370 GGCGCC GG^CGCC
NdeI 3166 CATATG CA^TATG
SexAI 668 ACCAGGT A^CC[AT]GGT
SfoI 1370 GGCGCC GGC^GCC
SmaI 1726 CCCGGG CCC^GGG
SmlI 1739 CTCAAG C^T[CT][GA]AG
StuI 2999 AGGCCT AGG^CCT
TaqI 2156 TCGA T^CGA
Tru9I 2720 TTAA T^TAA
TthHB8I 2156 TCGA T^CGA
XmaCI 1726 CCCGGG C^CCGGG
XmaI 1726 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 2087 TCCGGA T^CCGGA
2957 TCCGGA
AfaI 135 GTAC GT^AC
1567 GTAC
AocI 484 CCTGAGG CC^T[ACGT]AGG
1006 CCTCAGG
Aor51HI 999 AGCGCT AGC^GCT
3204 AGCGCT
AviII 962 TGCGCA TGC^GCA
3162 TGCGCA
BciVI 2673 GGATAC GTATCC(6/5)
1058 GTATCC
BsaWI 2087 TCCGGA [AT]^CCGG[AT]
2957 TCCGGA
BseAI 2087 TCCGGA T^CCGGA
2957 TCCGGA
BsmI 1440 GAATGC GAATGC(1/-1)
658 GCATTC
BspEI 2087 TCCGGA T^CCGGA
2957 TCCGGA
BsrDI 2532 CATTGC GCAATG(2/0)
2645 GCAATG
BsrFI 677 ACCGGC [GA]^CCGG[CT]
2989 GCCGGT
Bsu36I 484 CCTGAGG CC^T[ACGT]AGG
1006 CCTCAGG
Cfr10I 677 ACCGGC [GA]^CCGG[CT]
2989 GCCGGT
DrdI 1756 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3024 GACAGGGAAGTC
Eco47III 999 AGCGCT AGC^GCT
3204 AGCGCT
Eco81I 484 CCTGAGG CC^T[ACGT]AGG
1006 CCTCAGG
FspI 962 TGCGCA TGC^GCA
3162 TGCGCA
MroI 2087 TCCGGA T^CCGGA
2957 TCCGGA
NcoI 1280 CCATGG C^CATGG
2263 CCATGG
PpuMI 1656 GGGTCCT [GA]G^G[AT]CC[CT]
2270 AGGACCT
RsaI 135 GTAC GT^AC
1567 GTAC
SapI 3150 GAAGAGC GCTCTTC(1/4)
3171 GAAGAGC
Tsp509I 595 AATT ^AATT
1393 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I TspRI
Van91I XcmI XhoII XmnI