Restriction map for
pFN21AB9471
(Circular) (Six-base) MAPSORT of: pFN21AB9471.seq Check: 1450 from: 1 to: 8002
pFN21AB9471 8002 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2266 3325 3912 4624 2266
Size: 1059 587 712 5644
Fragments arranged by size:
5644 1059 712 587
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5812 278
Size: 53 83 186 936 4276 2468
Fragments arranged by size:
4276 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3873 5345 1937
Size: 1936 1472 4594
Fragments arranged by size:
4594 1936 1472
Acc65I G'GTAC_C
Cuts at: 3571 5323 3571
Size: 1752 6250
AclI AA'CG_TT
Cuts at: 1563 6129 6502 1563
Size: 4566 373 3063
Fragments arranged by size:
4566 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 3210 3489 4128 4457 5240 6065 7449
Size: 887 691 279 639 329 783 825 1384
Cuts at: 7449 7717 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 887 825 783 691 639 329
279 268
AfeI AGC'GCT
Cuts at: 3006 5211 3006
Size: 2205 5797
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7805
AflIII A'CryG_T
Cuts at: 1203 6901 7878 1203
Size: 5698 977 1327
Fragments arranged by size:
5698 1327 977
AgeI A'CCGG_T
Cuts at: 6833 6833
Size: 8002
AhdI GACnn_n'nnGTC
Cuts at: 6731 6731
Size: 8002
AleI CACnn'nnGTG
Cuts at: 1166 4151 1166
Size: 2985 5017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8002
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 7970
AlwNI CAG_nnn'CTG
Cuts at: 2364 2638 3232 3593 4372 4493 4576 5269 5745
Size: 274 594 361 779 121 83 693 476
Cuts at: 5745 7317 2364
Size: 1572 3049
Fragments arranged by size:
3049 1572 779 693 594 476 361 274
121 83
ApaI G_GGCC'C
Cuts at: 2327 2522 2705 2327
Size: 195 183 7624
Fragments arranged by size:
7624 195 183
ApaLI G'TGCA_C
Cuts at: 6059 7215 6059
Size: 1156 6846
ApoI r'AATT_y
Cuts at: 1493 2599 5311 5537 5689 7798 1493
Size: 1106 2712 226 152 2109 1697
Fragments arranged by size:
2712 2109 1697 1106 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6556 160
Size: 872 5524 1606
Fragments arranged by size:
5524 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 3731 3821 5327 1870
Size: 73 201 1587 90 1506 4545
Fragments arranged by size:
4545 1587 1506 201 90 73
BamHI G'GATC_C
Cuts at: 5332 5699 5332
Size: 367 7635
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 2923 3095 3375 3571
Size: 325 217 627 803 333 172 280 196
Cuts at: 3571 4358 4750 4840 5323 6778 618
Size: 787 392 90 483 1455 1842
Fragments arranged by size:
1842 1455 803 787 627 483 392 333
325 280 217 196 172 90
BanII G_rGCy'C
Cuts at: 1391 2327 2450 2522 2705 3828 4184 4797 4818
Size: 936 123 72 183 1123 356 613 21
Cuts at: 4818 5321 1391
Size: 503 4072
Fragments arranged by size:
4072 1123 936 613 503 356 183 123
72 21
BbeI G_GCGC'C
Cuts at: 3379 3379
Size: 8002
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2683 3564 4320 928
Size: 971 784 881 756 4610
Fragments arranged by size:
4610 971 881 784 756
BbvCI CC'TCA_GC
Cuts at: 2254 3215 3983 5213 2254
Size: 961 768 1230 5043
Fragments arranged by size:
5043 1230 961 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 6193 6227 1903
Size: 34 1904 34 2318 34 3678
Fragments arranged by size:
3678 2318 1904 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 4672 5894 7104 3074
Size: 1598 1222 1210 3972
Fragments arranged by size:
3972 1598 1222 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2884 3419 4450
Size: 107 122 71 631 605 1212 535 1031
Cuts at: 4450 6613 136
Size: 2163 1525
Fragments arranged by size:
2163 1525 1212 1031 631 605 535 122
107 71
BglII A'GATC_T
Cuts at: 7997 7997
Size: 8002
BlpI GC'TnA_GC
Cuts at: 3681 5419 3681
Size: 1738 6264
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2327 2522 2705 3854 4078 4225 5050
Size: 361 176 195 183 1149 224 147 825
Cuts at: 5050 6063 7219 1790
Size: 1013 1156 2573
Fragments arranged by size:
2573 1156 1149 1013 825 361 224 195
183 176 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8002
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2900 3581 4145
Size: 416 53 83 399 24 1483 681 564
Cuts at: 4145 4594 5733 6691 442
Size: 449 1139 958 1753
Fragments arranged by size:
1753 1483 1139 958 681 564 449 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8002
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 3451 2269
Size: 1182 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1997 2298 2478 2591 3230 3657 4321 4811
Size: 758 301 180 113 639 427 664 490
Cuts at: 4811 6662 1239
Size: 1851 2579
Fragments arranged by size:
2579 1851 758 664 639 490 427 301
180 113
Bpu10I CC'TnA_GC
Cuts at: 2244 2254 3215 3535 3750 3983 5213 2244
Size: 10 961 320 215 233 1230 5033
Fragments arranged by size:
5033 1230 961 320 233 215 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3729 6127 6992 7290 3729
Size: 2398 865 298 4441
Fragments arranged by size:
4441 2398 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6665 882
Size: 617 5166 2219
Fragments arranged by size:
5166 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5793 493
Size: 711 4589 2702
Fragments arranged by size:
4589 2702 711
BsaBI GATnn'nnATC
Cuts at: 7996 7996
Size: 8002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 5809 6191 6826
Size: 53 83 186 936 1843 2433 382 635
Cuts at: 6826 275
Size: 1451
Fragments arranged by size:
2433 1843 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4962 6435 6833 7107 7254 7665 4092
Size: 870 1473 398 274 147 411 4429
Fragments arranged by size:
4429 1473 870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 2154 2184 2451
Size: 30 165 30 720 30 1 30 267
Cuts at: 2451 2481 4166 4196 1178
Size: 30 1685 30 4984
Fragments arranged by size:
4984 1685 720 267 165 30 30 30
30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2352 3311 4044 4416 4847 4850 4877
Size: 961 205 959 733 372 431 3 27
Cuts at: 4877 5291 1186
Size: 414 3897
Fragments arranged by size:
3897 961 959 733 431 414 372 205
27 3
BseYI C'CCAG_C
Cuts at: 2019 2109 2271 2882 3074 3099 7205 7701 2019
Size: 90 162 611 192 25 4106 496 2320
Fragments arranged by size:
4106 2320 611 496 192 162 90 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2585 4364 4373 4589 1163
Size: 573 849 1779 9 216 4576
Fragments arranged by size:
4576 1779 849 573 216 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4648 5452 6213 7241 1540
Size: 275 186 2647 804 761 1028 2301
Fragments arranged by size:
2647 2301 1028 804 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3320 3365 3553 3687 6063 6148 7219 3320
Size: 45 188 134 2376 85 1071 4103
Fragments arranged by size:
4103 2376 1071 188 134 85 45
BsmI GAATG_Cn'
Cuts at: 2663 3451 5513 5606 2663
Size: 788 2062 93 5059
Fragments arranged by size:
5059 2062 788 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2327 2450 2522 2705 3320 3365
Size: 399 361 176 123 72 183 615 45
Cuts at: 3365 3553 3687 3828 3854 4078 4184 4225 4797
Size: 188 134 141 26 224 106 41 572
Cuts at: 4797 4818 5050 5321 6063 6148 7219 1391
Size: 21 232 271 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 572 399 361 271
232 224 188 183 176 141 134 123
106 85 72 45 41 26 21
BspEI T'CCGG_A
Cuts at: 4092 4962 7665 4092
Size: 870 2703 4429
Fragments arranged by size:
4429 2703 870
BspHI T'CATG_A
Cuts at: 5891 7621 5891
Size: 1730 6272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2266 2677 3325 3912 4326 4624 5357 844
Size: 1422 411 648 587 414 298 733 3489
Fragments arranged by size:
3489 1422 733 648 587 414 411 298
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 2812 3039 3943 5283 5889 1394
Size: 429 425 564 227 904 1340 606 3507
Fragments arranged by size:
3507 1340 904 606 564 429 425 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 4536 4657 6497 6671 65
Size: 1183 3288 121 1840 174 1396
Fragments arranged by size:
3288 1840 1396 1183 174 121
BsrFI r'CCGG_y
Cuts at: 1700 1810 2682 4994 6646 6833 1700
Size: 110 872 2312 1652 187 2869
Fragments arranged by size:
2869 2312 1652 872 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8002
BssSI C'ACGA_G
Cuts at: 2150 3552 4970 6062 7074 2150
Size: 1402 1418 1092 1012 3078
Fragments arranged by size:
3078 1418 1402 1092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3232 4387 5269 794
Size: 2438 1155 882 3527
Fragments arranged by size:
3527 2438 1155 882
BstBI TT'CG_AA
Cuts at: 6806 6806
Size: 8002
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8002
BstXI CCAn_nnnn'nTGG
Cuts at: 2867 3082 4594 2867
Size: 215 1512 6275
Fragments arranged by size:
6275 1512 215
BstYI r'GATC_y
Cuts at: 1972 3021 4237 4848 5332 5699 6084 6101 7553
Size: 1049 1216 611 484 367 385 17 1452
Cuts at: 7553 7639 7651 7997 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1216 1049 611 484 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2490 3012 2490
Size: 522 7480
BtgI C'CryG_G
Cuts at: 513 1065 3285 4166 4268 4593 513
Size: 552 2220 881 102 325 3922
Fragments arranged by size:
3922 2220 881 552 325 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3844 4064 4701 487
Size: 683 56 2618 220 637 3788
Fragments arranged by size:
3788 2618 683 637 220 56
BtsI GCAGTG_nn'
Cuts at: 2636 3318 3437 3466 5170 5518 6312 6332 2636
Size: 682 119 29 1704 348 794 20 4306
Fragments arranged by size:
4306 1704 794 682 348 119 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2703 2738 529
Size: 35 2139 35 5793
Fragments arranged by size:
5793 2139 35 35
DraI TTT'AAA
Cuts at: 5306 5306
Size: 8002
DraIII CAC_nnn'GTG
Cuts at: 2677 2677
Size: 8002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3767 5035 7009 809
Size: 507 2451 1268 1974 1802
Fragments arranged by size:
2451 1974 1802 1268 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2668 3262 4509
Size: 54 1425 50 275 18 838 594 1247
Cuts at: 4509 4645 4941 4999 5449 6338 7967 8
Size: 136 296 58 450 889 1629 43
Fragments arranged by size:
1629 1425 1247 889 838 594 450 296
275 136 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4645 5449 1537
Size: 275 2833 804 4090
Fragments arranged by size:
4090 2833 804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3465 4701 4759 4761 4776 4866 5150
Size: 707 1405 1236 58 2 15 90 284
Cuts at: 5150 5171 5932 1353
Size: 21 761 3423
Fragments arranged by size:
3423 1405 1236 761 707 284 90 58
21 15 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3363 6589 6963 7109 387
Size: 1064 138 1774 3226 374 146 1280
Fragments arranged by size:
3226 1774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1997 2298 2478 2519 2591 3210 3230
Size: 393 365 301 180 41 72 619 20
Cuts at: 3230 3489 3657 4128 4321 4457 4811 5240 6065
Size: 259 168 471 193 136 354 429 825
Cuts at: 6065 6662 7449 7717 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 825 787 619 597 471 429 393
365 354 301 268 259 193 180 168
136 72 41 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 3053 4210 5273 2981
Size: 72 1157 1063 5710
Fragments arranged by size:
5710 1157 1063 72
EcoO109I rG'GnC_Cy
Cuts at: 2323 2518 2701 2974 3662 4276 5266 2323
Size: 195 183 273 688 614 990 5059
Fragments arranged by size:
5059 990 688 614 273 195 183
EcoRI G'AATT_C
Cuts at: 2599 5311 5689 2599
Size: 2712 378 4912
Fragments arranged by size:
4912 2712 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4270 4576 4270
Size: 306 7696
FspI TGC'GCA
Cuts at: 2969 5169 6508 2969
Size: 2200 1339 4463
Fragments arranged by size:
4463 2200 1339
FspAI rTGC'GCAy
Cuts at: 5169 5169
Size: 8002
HaeII r_GCGC'y
Cuts at: 1678 3008 3081 3379 5213 7149 1678
Size: 1330 73 298 1834 1936 2531
Fragments arranged by size:
2531 1936 1834 1330 298 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 2437 2451 2469
Size: 32 135 32 582 32 251 14 18
Cuts at: 2469 2483 3727 3759 3920 3952 4063 4095 4392
Size: 14 1244 32 161 32 111 32 297
Cuts at: 4392 4424 4679 4711 5086 5118 6643 6675 6717
Size: 32 255 32 375 32 1525 32 42
Cuts at: 6717 6749 1373
Size: 32 2626
Fragments arranged by size:
2626 1525 1244 582 375 297 255 251
161 135 111 42 32 32 32 32
32 32 32 32 32 32 32 18
14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5346 5592 669
Size: 988 281 3408 246 3079
Fragments arranged by size:
3408 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8002
HpaI GTT'AAC
Cuts at: 5592 5592
Size: 8002
KasI G'GCGC_C
Cuts at: 3375 3375
Size: 8002
KpnI G_GTAC'C
Cuts at: 3575 5327 3575
Size: 1752 6250
MfeI C'AATT_G
Cuts at: 5601 5601
Size: 8002
MluI A'CGCG_T
Cuts at: 7878 7878
Size: 8002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3756 3903 5001 7116 7300 1305
Size: 2451 147 1098 2115 184 2007
Fragments arranged by size:
2451 2115 2007 1098 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5001 10
Size: 54 1425 343 3169 3011
Fragments arranged by size:
3169 3011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3587 4151 5960 6319 6478 518
Size: 648 957 1464 564 1809 359 159 2042
Fragments arranged by size:
2042 1809 1464 957 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2364 2527 2569 2659 2882 3208 3811 4232
Size: 758 163 42 90 223 326 603 421
Cuts at: 4232 4468 4901 5237 5418 6095 7243 7488 1606
Size: 236 433 336 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 677 603 433 421 336
326 245 236 223 181 163 90 42
NarI GG'CG_CC
Cuts at: 3376 3376
Size: 8002
NcoI C'CATG_G
Cuts at: 513 1065 3285 4268 513
Size: 552 2220 983 4247
Fragments arranged by size:
4247 2220 983 552
NdeI CA'TA_TG
Cuts at: 387 5172 387
Size: 4785 3217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8002
NotI GC'GGCC_GC
Cuts at: 5449 5449
Size: 8002
NspI r_CATG'y
Cuts at: 1132 1229 5360 6905 1132
Size: 97 4131 1545 2229
Fragments arranged by size:
4131 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3172 4985 1348
Size: 1824 1813 4365
Fragments arranged by size:
4365 1824 1813
PciI A'CATG_T
Cuts at: 6901 6901
Size: 8002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 2920 4372 4468 4606 1108
Size: 1170 642 1452 96 138 4504
Fragments arranged by size:
4504 1452 1170 642 138 96
PfoI T'CCnGG_A
Cuts at: 2158 2455 4410 2158
Size: 297 1955 5750
Fragments arranged by size:
5750 1955 297
PmeI GTTT'AAAC
Cuts at: 5306 5306
Size: 8002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6071 6103 7584 7616 6071
Size: 32 1481 32 6457
Fragments arranged by size:
6457 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3662 4276 3662
Size: 614 7388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8002
PsiI TTA'TAA
Cuts at: 5572 5572
Size: 8002
PspOMI G'GGCC_C
Cuts at: 2323 2518 2701 2323
Size: 195 183 7624
Fragments arranged by size:
7624 195 183
PstI C_TGCA'G
Cuts at: 830 2103 2877 2985 3153 3444 4237 5209 5354
Size: 1273 774 108 168 291 793 972 145
Cuts at: 5354 830
Size: 3478
Fragments arranged by size:
3478 1273 972 793 774 291 168 145
108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8002
PvuII CAG'CTG
Cuts at: 2364 2527 2569 2659 2882 4232 2364
Size: 163 42 90 223 1350 6134
Fragments arranged by size:
6134 1350 223 163 90 42
SalI G'TCGA_C
Cuts at: 1936 5344 1936
Size: 3408 4594
SapI GCTCTTCn'nnn_
Cuts at: 5150 5171 5150
Size: 21 7981
SbfI CC_TGCA'GG
Cuts at: 5354 5354
Size: 8002
ScaI AGT'ACT
Cuts at: 6250 7962 6250
Size: 1712 6290
SexAI A'CCwGG_T
Cuts at: 2673 2673
Size: 8002
SfcI C'TryA_G
Cuts at: 826 1045 2099 2428 2873 2981 3149 3440 4233
Size: 219 1054 329 445 108 168 291 793
Cuts at: 4233 4925 5205 5350 6485 7166 7357 826
Size: 692 280 145 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 793 692 681 445 329
291 280 219 191 168 145 108
SfoI GGC'GCC
Cuts at: 3377 3377
Size: 8002
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8002
SmaI CCC'GGG
Cuts at: 1872 3733 5329 1872
Size: 1861 1596 4545
Fragments arranged by size:
4545 1861 1596
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3744 6106 7007 7269 820
Size: 197 926 1801 2362 901 262 1553
Fragments arranged by size:
2362 1801 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5793 493
Size: 5300 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8002
SphI G_CATG'C
Cuts at: 1132 5360 1132
Size: 4228 3774
SspI AAT'ATT
Cuts at: 5 52 5926 5
Size: 47 5874 2081
Fragments arranged by size:
5874 2081 47
StuI AGG'CCT
Cuts at: 1517 5006 1517
Size: 3489 4513
StyI C'CwwG_G
Cuts at: 513 1065 2025 2079 2439 3285 3843 4268 4745
Size: 552 960 54 360 846 558 425 477
Cuts at: 4745 5018 5441 513
Size: 273 423 3074
Fragments arranged by size:
3074 960 846 558 552 477 425 423
360 273 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3271 6029 6046 6199 6384 6829
Size: 1255 1533 158 2758 17 153 185 445
Cuts at: 6829 325
Size: 1498
Fragments arranged by size:
2758 1533 1498 1255 445 185 158 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6248 7960 96
Size: 274 80 33 51 1443 4271 1712 138
Fragments arranged by size:
4271 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 4087 4119 7962 7994 2405
Size: 32 1650 32 3843 32 2413
Fragments arranged by size:
3843 2413 1650 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8002
XbaI T'CTAG_A
Cuts at: 5338 5338
Size: 8002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 3511 4369 2305
Size: 1206 858 5938
Fragments arranged by size:
5938 1206 858
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8002
XmaI C'CCGG_G
Cuts at: 1870 3731 5327 1870
Size: 1861 1596 4545
Fragments arranged by size:
4545 1861 1596
XmnI GAAnn'nnTTC
Cuts at: 2603 4523 4556 5114 6131 2603
Size: 1920 33 558 1017 4474
Fragments arranged by size:
4474 1920 1017 558 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5810 276
Size: 53 83 186 936 4276 2468
Fragments arranged by size:
4276 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI
EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI
SwaI