Restriction map for
pFN21AB9471
(Linear) (Six-base) MAPSORT of: KIBB9471.seq Check: 6587 from: 1 to: 3297
KIBB9471.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 261 1320 1907 2619 3297
Size: 261 1059 587 712 678
Fragments arranged by size:
1059 712 678 587 261
AccI GT'mk_AC
Cuts at: 0 1868 3297
Size: 1868 1429
Acc65I G'GTAC_C
Cuts at: 0 1566 3297
Size: 1566 1731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1205 1484 2123 2452 3235 3297
Size: 514 691 279 639 329 783 62
Fragments arranged by size:
783 691 639 514 329 279 62
AfeI AGC'GCT
Cuts at: 0 1001 3206 3297
Size: 1001 2205 91
Fragments arranged by size:
2205 1001 91
AleI CACnn'nnGTG
Cuts at: 0 2146 3297
Size: 2146 1151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 808 840 3297
Size: 808 32 2457
Fragments arranged by size:
2457 808 32
AlwNI CAG_nnn'CTG
Cuts at: 0 359 633 1227 1588 2367 2488 2571 3264
Size: 359 274 594 361 779 121 83 693
Cuts at: 3264 3297
Size: 33
Fragments arranged by size:
779 693 594 361 359 274 121 83
33
ApaI G_GGCC'C
Cuts at: 0 322 517 700 3297
Size: 322 195 183 2597
Fragments arranged by size:
2597 322 195 183
ApoI r'AATT_y
Cuts at: 0 594 3297
Size: 594 2703
AvaI C'yCGr_G
Cuts at: 0 139 1726 1816 3297
Size: 139 1587 90 1481
Fragments arranged by size:
1587 1481 139 90
BanI G'GyrC_C
Cuts at: 0 585 918 1090 1370 1566 2353 2745 2835
Size: 585 333 172 280 196 787 392 90
Cuts at: 2835 3297
Size: 462
Fragments arranged by size:
787 585 462 392 333 280 196 172
90
BanII G_rGCy'C
Cuts at: 0 322 445 517 700 1823 2179 2792 2813
Size: 322 123 72 183 1123 356 613 21
Cuts at: 2813 3297
Size: 484
Fragments arranged by size:
1123 613 484 356 322 183 123 72
21
BbeI G_GCGC'C
Cuts at: 0 1374 3297
Size: 1374 1923
BbsI GAAGACnn'nnnn_
Cuts at: 0 678 1559 2315 3297
Size: 678 881 756 982
Fragments arranged by size:
982 881 756 678
BbvCI CC'TCA_GC
Cuts at: 0 249 1210 1978 3208 3297
Size: 249 961 768 1230 89
Fragments arranged by size:
1230 961 768 249 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1836 1870 3297
Size: 1836 34 1427
Fragments arranged by size:
1836 1427 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1069 2667 3297
Size: 1069 1598 630
Fragments arranged by size:
1598 1069 630
BglI GCCn_nnn'nGGC
Cuts at: 0 879 1414 2445 3297
Size: 879 535 1031 852
Fragments arranged by size:
1031 879 852 535
BlpI GC'TnA_GC
Cuts at: 0 1676 3297
Size: 1676 1621
Bme1580I G_kGCm'C
Cuts at: 0 146 322 517 700 1849 2073 2220 3045
Size: 146 176 195 183 1149 224 147 825
Cuts at: 3045 3297
Size: 252
Fragments arranged by size:
1149 825 252 224 195 183 176 147
146
BmrI ACTGGGnnnn_n'
Cuts at: 0 895 1576 2140 2589 3297
Size: 895 681 564 449 708
Fragments arranged by size:
895 708 681 564 449
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 1446 3297
Size: 264 1182 1851
Fragments arranged by size:
1851 1182 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 473 586 1225 1652 2316 2806 3297
Size: 293 180 113 639 427 664 490 491
Fragments arranged by size:
664 639 491 490 427 293 180 113
Bpu10I CC'TnA_GC
Cuts at: 0 239 249 1210 1530 1745 1978 3208 3297
Size: 239 10 961 320 215 233 1230 89
Fragments arranged by size:
1230 961 320 239 233 215 89 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 3297
Size: 1724 1573
BsaHI Gr'CG_yC
Cuts at: 0 1371 3297
Size: 1371 1926
BsaWI w'CCGG_w
Cuts at: 0 2087 2957 3297
Size: 2087 870 340
Fragments arranged by size:
2087 870 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 149 179 446 476 2161 2191
Size: 118 30 1 30 267 30 1685 30
Cuts at: 2191 3297
Size: 1106
Fragments arranged by size:
1685 1106 267 118 30 30 30 30
1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 347 1306 2039 2411 2842 2845 2872
Size: 142 205 959 733 372 431 3 27
Cuts at: 2872 3286 3297
Size: 414 11
Fragments arranged by size:
959 733 431 414 372 205 142 27
11 3
BseYI C'CCAG_C
Cuts at: 0 14 104 266 877 1069 1094 3297
Size: 14 90 162 611 192 25 2203
Fragments arranged by size:
2203 611 192 162 90 25 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 2359 2368 2584 3297
Size: 580 1779 9 216 713
Fragments arranged by size:
1779 713 580 216 9
BsiEI CG_ry'CG
Cuts at: 0 2643 3297
Size: 2643 654
BsiHKAI G_wGCw'C
Cuts at: 0 1315 1360 1548 1682 3297
Size: 1315 45 188 134 1615
Fragments arranged by size:
1615 1315 188 134 45
BsmI GAATG_Cn'
Cuts at: 0 658 1446 3297
Size: 658 788 1851
Fragments arranged by size:
1851 788 658
Bsp1286I G_dGCh'C
Cuts at: 0 146 322 445 517 700 1315 1360 1548
Size: 146 176 123 72 183 615 45 188
Cuts at: 1548 1682 1823 1849 2073 2179 2220 2792 2813
Size: 134 141 26 224 106 41 572 21
Cuts at: 2813 3045 3297
Size: 232 252
Fragments arranged by size:
615 572 252 232 224 188 183 176
146 141 134 123 106 72 45 41
26 21
BspEI T'CCGG_A
Cuts at: 0 2087 2957 3297
Size: 2087 870 340
Fragments arranged by size:
2087 870 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 672 1320 1907 2321 2619 3297
Size: 261 411 648 587 414 298 678
Fragments arranged by size:
678 648 587 414 411 298 261
BsrBI CCG'CTC
Cuts at: 0 243 807 1034 1938 3278 3297
Size: 243 564 227 904 1340 19
Fragments arranged by size:
1340 904 564 243 227 19
BsrDI GCAATG_nn'
Cuts at: 0 2531 2652 3297
Size: 2531 121 645
Fragments arranged by size:
2531 645 121
BsrFI r'CCGG_y
Cuts at: 0 677 2989 3297
Size: 677 2312 308
Fragments arranged by size:
2312 677 308
BssSI C'ACGA_G
Cuts at: 0 145 1547 2965 3297
Size: 145 1402 1418 332
Fragments arranged by size:
1418 1402 332 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1227 2382 3264 3297
Size: 1227 1155 882 33
Fragments arranged by size:
1227 1155 882 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 862 1077 2589 3297
Size: 862 215 1512 708
Fragments arranged by size:
1512 862 708 215
BstYI r'GATC_y
Cuts at: 0 1016 2232 2843 3297
Size: 1016 1216 611 454
Fragments arranged by size:
1216 1016 611 454
Bsu36I CC'TnA_GG
Cuts at: 0 485 1007 3297
Size: 485 522 2290
Fragments arranged by size:
2290 522 485
BtgI C'CryG_G
Cuts at: 0 1280 2161 2263 2588 3297
Size: 1280 881 102 325 709
Fragments arranged by size:
1280 881 709 325 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1839 2059 2696 3297
Size: 1839 220 637 601
Fragments arranged by size:
1839 637 601 220
BtsI GCAGTG_nn'
Cuts at: 0 631 1313 1432 1461 3165 3297
Size: 631 682 119 29 1704 132
Fragments arranged by size:
1704 682 631 132 119 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 698 733 3297
Size: 698 35 2564
Fragments arranged by size:
2564 698 35
DraIII CAC_nnn'GTG
Cuts at: 0 672 3297
Size: 672 2625
DrdI GACnn_nn'nnGTC
Cuts at: 0 1762 3030 3297
Size: 1762 1268 267
Fragments arranged by size:
1762 1268 267
EaeI y'GGCC_r
Cuts at: 0 663 1257 2504 2640 2936 2994 3297
Size: 663 594 1247 136 296 58 303
Fragments arranged by size:
1247 663 594 303 296 136 58
EagI C'GGCC_G
Cuts at: 0 2640 3297
Size: 2640 657
EarI CTCTTCn'nnn_
Cuts at: 0 55 1460 2696 2754 2756 2771 2861 3145
Size: 55 1405 1236 58 2 15 90 284
Cuts at: 3145 3166 3297
Size: 21 131
Fragments arranged by size:
1405 1236 284 131 90 58 55 21
15 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1358 3297
Size: 1358 1939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 473 514 586 1205 1225 1484 1652
Size: 293 180 41 72 619 20 259 168
Cuts at: 1652 2123 2316 2452 2806 3235 3297
Size: 471 193 136 354 429 62
Fragments arranged by size:
619 471 429 354 293 259 193 180
168 136 72 62 41 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 1048 2205 3268 3297
Size: 976 72 1157 1063 29
Fragments arranged by size:
1157 1063 976 72 29
EcoO109I rG'GnC_Cy
Cuts at: 0 318 513 696 969 1657 2271 3261 3297
Size: 318 195 183 273 688 614 990 36
Fragments arranged by size:
990 688 614 318 273 195 183 36
EcoRI G'AATT_C
Cuts at: 0 594 3297
Size: 594 2703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2265 2571 3297
Size: 2265 306 726
Fragments arranged by size:
2265 726 306
FspI TGC'GCA
Cuts at: 0 964 3164 3297
Size: 964 2200 133
Fragments arranged by size:
2200 964 133
FspAI rTGC'GCAy
Cuts at: 0 3164 3297
Size: 3164 133
HaeII r_GCGC'y
Cuts at: 0 1003 1076 1374 3208 3297
Size: 1003 73 298 1834 89
Fragments arranged by size:
1834 1003 298 89 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 432 446 464 478 1722 1754
Size: 149 32 251 14 18 14 1244 32
Cuts at: 1754 1915 1947 2058 2090 2387 2419 2674 2706
Size: 161 32 111 32 297 32 255 32
Cuts at: 2706 3081 3113 3297
Size: 375 32 184
Fragments arranged by size:
1244 375 297 255 251 184 161 149
111 32 32 32 32 32 32 32
18 14 14
KasI G'GCGC_C
Cuts at: 0 1370 3297
Size: 1370 1927
KpnI G_GTAC'C
Cuts at: 0 1570 3297
Size: 1570 1727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1751 1898 2996 3297
Size: 1751 147 1098 301
Fragments arranged by size:
1751 1098 301 147
MscI TGG'CCA
Cuts at: 0 2996 3297
Size: 2996 301
MslI CAynn'nnrTG
Cuts at: 0 118 1582 2146 3297
Size: 118 1464 564 1151
Fragments arranged by size:
1464 1151 564 118
MspA1I CmG'CkG
Cuts at: 0 359 522 564 654 877 1203 1806 2227
Size: 359 163 42 90 223 326 603 421
Cuts at: 2227 2463 2896 3232 3297
Size: 236 433 336 65
Fragments arranged by size:
603 433 421 359 336 326 236 223
163 90 65 42
NarI GG'CG_CC
Cuts at: 0 1371 3297
Size: 1371 1926
NcoI C'CATG_G
Cuts at: 0 1280 2263 3297
Size: 1280 983 1034
Fragments arranged by size:
1280 1034 983
NdeI CA'TA_TG
Cuts at: 0 3167 3297
Size: 3167 130
PasI CC'CwG_GG
Cuts at: 0 1167 2980 3297
Size: 1167 1813 317
Fragments arranged by size:
1813 1167 317
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 915 2367 2463 2601 3297
Size: 273 642 1452 96 138 696
Fragments arranged by size:
1452 696 642 273 138 96
PfoI T'CCnGG_A
Cuts at: 0 153 450 2405 3297
Size: 153 297 1955 892
Fragments arranged by size:
1955 892 297 153
PpuMI rG'GwC_Cy
Cuts at: 0 1657 2271 3297
Size: 1657 614 1026
Fragments arranged by size:
1657 1026 614
PspOMI G'GGCC_C
Cuts at: 0 318 513 696 3297
Size: 318 195 183 2601
Fragments arranged by size:
2601 318 195 183
PstI C_TGCA'G
Cuts at: 0 98 872 980 1148 1439 2232 3204 3297
Size: 98 774 108 168 291 793 972 93
Fragments arranged by size:
972 793 774 291 168 108 98 93
PvuII CAG'CTG
Cuts at: 0 359 522 564 654 877 2227 3297
Size: 359 163 42 90 223 1350 1070
Fragments arranged by size:
1350 1070 359 223 163 90 42
SapI GCTCTTCn'nnn_
Cuts at: 0 3145 3166 3297
Size: 3145 21 131
Fragments arranged by size:
3145 131 21
SexAI A'CCwGG_T
Cuts at: 0 668 3297
Size: 668 2629
SfcI C'TryA_G
Cuts at: 0 94 423 868 976 1144 1435 2228 2920
Size: 94 329 445 108 168 291 793 692
Cuts at: 2920 3200 3297
Size: 280 97
Fragments arranged by size:
793 692 445 329 291 280 168 108
97 94
SfoI GGC'GCC
Cuts at: 0 1372 3297
Size: 1372 1925
SmaI CCC'GGG
Cuts at: 0 1728 3297
Size: 1728 1569
SmlI C'TyrA_G
Cuts at: 0 1739 3297
Size: 1739 1558
StuI AGG'CCT
Cuts at: 0 3001 3297
Size: 3001 296
StyI C'CwwG_G
Cuts at: 0 20 74 434 1280 1838 2263 2740 3013
Size: 20 54 360 846 558 425 477 273
Cuts at: 3013 3297
Size: 284
Fragments arranged by size:
846 558 477 425 360 284 273 54
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 1266 3297
Size: 1108 158 2031
Fragments arranged by size:
2031 1108 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 2082 2114 3297
Size: 400 32 1650 32 1183
Fragments arranged by size:
1650 1183 400 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 1506 2364 3297
Size: 300 1206 858 933
Fragments arranged by size:
1206 933 858 300
XmaI C'CCGG_G
Cuts at: 0 1726 3297
Size: 1726 1571
XmnI GAAnn'nnTTC
Cuts at: 0 598 2518 2551 3109 3297
Size: 598 1920 33 558 188
Fragments arranged by size:
1920 598 558 188 33
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NdeI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SapI SexAI SfcI SfoI SmaI SmlI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI
BglII BmgBI BmtI BsaI BsaAI BsaBI BsiWI BsmBI
BspHI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI
EcoICRI EcoRV FseI HincII HindIII HpaI MfeI MluI
NaeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI ZraI