(Circular) (Six-base) MAPSORT of: pFN21AB9469.seq Check: 5659 from: 1 to: 7111 pFN21AB9469 7111 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4454 1937 Size: 2517 4594 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2978 5174 6558 6826 1632 Size: 1346 2196 1384 268 1917 Fragments arranged by size: 2196 1917 1384 1346 268 AfeI AGC'GCT Cuts at: 2149 3718 3785 2149 Size: 1569 67 5475 Fragments arranged by size: 5475 1569 67 AflII C'TTAA_G Cuts at: 820 1017 2750 820 Size: 197 1733 5181 Fragments arranged by size: 5181 1733 197 AflIII A'CryG_T Cuts at: 1203 6010 6987 1203 Size: 4807 977 1327 Fragments arranged by size: 4807 1327 977 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 3524 5840 3524 Size: 2316 4795 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AlwNI CAG_nnn'CTG Cuts at: 2034 2089 2194 3262 4091 4854 6426 2034 Size: 55 105 1068 829 763 1572 2719 Fragments arranged by size: 2719 1572 1068 829 763 105 55 ApaI G_GGCC'C Cuts at: 2943 3594 4098 2943 Size: 651 504 5956 Fragments arranged by size: 5956 651 504 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 2105 2180 2270 2356 2402 3353 3644 4420 Size: 612 75 90 86 46 951 291 776 Cuts at: 4420 4646 4798 6907 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 776 612 291 226 152 90 86 75 46 AseI AT'TA_AT Cuts at: 160 1032 5665 160 Size: 872 4633 1606 Fragments arranged by size: 4633 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 2730 3741 4436 1870 Size: 73 787 1011 695 4545 Fragments arranged by size: 4545 1011 787 695 73 AvrII C'CTAG_G Cuts at: 3448 3448 Size: 7111 BamHI G'GATC_C Cuts at: 4441 4808 4441 Size: 367 6744 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4432 5887 618 Size: 325 217 627 2645 1455 1842 Fragments arranged by size: 2645 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2655 2943 3594 3610 3996 4098 4430 1391 Size: 1264 288 651 16 386 102 332 4072 Fragments arranged by size: 4072 1264 651 386 332 288 102 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2451 2984 928 Size: 971 552 533 5055 Fragments arranged by size: 5055 971 552 533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5302 5336 1903 Size: 34 3365 34 3678 Fragments arranged by size: 3678 3365 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5003 6213 5003 Size: 1210 5901 BclI T'GATC_A Cuts at: 1548 1548 Size: 7111 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 136 Size: 107 122 71 631 605 4050 1525 Fragments arranged by size: 4050 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3690 7106 3690 Size: 3416 3695 BlpI GC'TnA_GC Cuts at: 2931 4528 2931 Size: 1597 5514 Bme1580I G_kGCm'C Cuts at: 1790 2943 3594 4098 5172 6328 1790 Size: 1153 651 504 1074 1156 2573 Fragments arranged by size: 2573 1156 1153 1074 651 504 BmgBI CAC'GTC Cuts at: 1318 4012 1318 Size: 2694 4417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3331 4220 4842 Size: 416 53 83 399 24 1914 889 622 Cuts at: 4842 5800 442 Size: 958 1753 Fragments arranged by size: 1914 1753 958 889 622 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3430 4149 3430 Size: 719 6392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2111 2707 4179 4419 5771 1239 Size: 803 69 596 1472 240 1352 2579 Fragments arranged by size: 2579 1472 1352 803 596 240 69 Bpu10I CC'TnA_GC Cuts at: 3609 3609 Size: 7111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 4368 5236 6101 6399 2054 Size: 2314 868 865 298 2766 Fragments arranged by size: 2766 2314 868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 3191 3418 4120 5774 882 Size: 617 1380 312 227 702 1654 2219 Fragments arranged by size: 2219 1654 1380 702 617 312 227 BsaAI yAC'GTr Cuts at: 493 1204 4902 493 Size: 711 3698 2702 Fragments arranged by size: 3698 2702 711 BsaBI GATnn'nnATC Cuts at: 3638 7105 3638 Size: 3467 3644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2906 4918 5300 5935 Size: 53 83 186 936 1373 2012 382 635 Cuts at: 5935 275 Size: 1451 Fragments arranged by size: 2012 1451 1373 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2361 5544 5942 6216 6363 6774 2361 Size: 3183 398 274 147 411 2698 Fragments arranged by size: 3183 2698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2670 2700 1178 Size: 30 165 30 1267 30 5589 Fragments arranged by size: 5589 1267 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3943 1186 Size: 2757 4354 BseYI C'CCAG_C Cuts at: 6314 6810 6314 Size: 496 6615 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3705 1163 Size: 573 1969 4569 Fragments arranged by size: 4569 1969 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4561 5322 6350 1540 Size: 275 186 2560 761 1028 2301 Fragments arranged by size: 2560 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2655 3543 3610 3681 5172 5257 6328 2655 Size: 888 67 71 1491 85 1071 3438 Fragments arranged by size: 3438 1491 1071 888 85 71 67 BsmI GAATG_Cn' Cuts at: 3274 4622 4715 3274 Size: 1348 93 5670 Fragments arranged by size: 5670 1348 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2655 2943 3543 3594 3610 3681 3996 Size: 399 865 288 600 51 16 71 315 Cuts at: 3996 4098 4430 5172 5257 6328 1391 Size: 102 332 742 85 1071 2174 Fragments arranged by size: 2174 1071 865 742 600 399 332 315 288 102 85 71 51 16 BspEI T'CCGG_A Cuts at: 2361 6774 2361 Size: 4413 2698 BspHI T'CATG_A Cuts at: 3384 5000 6730 3384 Size: 1616 1730 3765 Fragments arranged by size: 3765 1730 1616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4466 844 Size: 3622 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2392 4998 1394 Size: 429 569 2606 3507 Fragments arranged by size: 3507 2606 569 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4309 5606 5780 65 Size: 1183 3061 1297 174 1396 Fragments arranged by size: 3061 1396 1297 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 5171 6183 5171 Size: 1012 6099 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7111 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7111 BstYI r'GATC_y Cuts at: 1972 3690 4240 4441 4808 5193 5210 6662 6748 Size: 1718 550 201 367 385 17 1452 86 Cuts at: 6748 6760 7106 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1718 1452 550 385 367 346 201 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3362 3708 3362 Size: 346 6765 BtgI C'CryG_G Cuts at: 513 1065 2004 3090 513 Size: 552 939 1086 4534 Fragments arranged by size: 4534 1086 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2236 487 Size: 683 56 1010 5362 Fragments arranged by size: 5362 1010 683 56 BtsI GCAGTG_nn' Cuts at: 4627 5421 5441 4627 Size: 794 20 6297 Fragments arranged by size: 6297 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4415 4415 Size: 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2197 6118 809 Size: 507 881 3921 1802 Fragments arranged by size: 3921 1802 881 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3624 4558 5447 Size: 54 1425 50 275 18 1794 934 889 Cuts at: 5447 7076 8 Size: 1629 43 Fragments arranged by size: 1794 1629 1425 934 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 5041 1353 Size: 1377 2311 3423 Fragments arranged by size: 3423 2311 1377 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3726 4208 5698 6072 6218 387 Size: 1064 138 2137 482 1490 374 146 1280 Fragments arranged by size: 2137 1490 1280 1064 482 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 3608 2653 Size: 955 6156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2111 2707 2978 4179 4419 5174 Size: 393 410 69 596 271 1201 240 755 Cuts at: 5174 5771 6558 6826 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1201 787 755 597 596 410 393 271 268 240 69 EcoO109I rG'GnC_Cy Cuts at: 2939 2940 3052 4233 2939 Size: 1 112 1181 5817 Fragments arranged by size: 5817 1181 112 1 EcoRI G'AATT_C Cuts at: 4420 4798 4420 Size: 378 6733 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3572 4409 3572 Size: 837 6274 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 2151 3720 3787 6258 1678 Size: 473 1569 67 2471 2531 Fragments arranged by size: 2531 2471 1569 473 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3510 3542 3592 3624 5752 Size: 32 135 32 1938 32 50 32 2128 Cuts at: 5752 5784 5826 5858 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2128 1938 135 50 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4455 4701 669 Size: 988 281 2517 246 3079 Fragments arranged by size: 3079 2517 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7111 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4710 4710 Size: 7111 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2713 3594 3871 3969 6225 6409 1305 Size: 1408 881 277 98 2256 184 2007 Fragments arranged by size: 2256 2007 1408 881 277 184 98 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5289 Fragments arranged by size: 5289 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3315 3599 3659 5069 5428 5587 518 Size: 648 2149 284 60 1410 359 159 2042 Fragments arranged by size: 2149 2042 1410 648 359 284 159 60 MspA1I CmG'CkG Cuts at: 1606 2020 4527 5204 6352 6597 1606 Size: 414 2507 677 1148 245 2120 Fragments arranged by size: 2507 2120 1148 677 414 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3090 513 Size: 552 939 1086 4534 Fragments arranged by size: 4534 1086 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 2784 4469 6014 1132 Size: 97 1555 1685 1545 2229 Fragments arranged by size: 2229 1685 1555 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 4091 1108 Size: 2983 4128 PfoI T'CCnGG_A Cuts at: 2559 2791 4195 4337 4364 4396 2559 Size: 232 1404 142 27 32 5274 Fragments arranged by size: 5274 1404 232 142 32 27 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3028 3060 5180 5212 6693 6725 3028 Size: 32 2120 32 1481 32 3414 Fragments arranged by size: 3414 2120 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 4681 4681 Size: 7111 PspOMI G'GGCC_C Cuts at: 2939 3590 4094 2939 Size: 651 504 5956 Fragments arranged by size: 5956 651 504 PstI C_TGCA'G Cuts at: 830 2199 3051 4038 4463 830 Size: 1369 852 987 425 3478 Fragments arranged by size: 3478 1369 987 852 425 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 SacI G_AGCT'C Cuts at: 2655 3610 2655 Size: 955 6156 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 2963 5359 7071 2963 Size: 2396 1712 3003 Fragments arranged by size: 3003 2396 1712 SexAI A'CCwGG_T Cuts at: 4343 4343 Size: 7111 SfcI C'TryA_G Cuts at: 826 1045 2195 2498 3047 3391 4034 4459 5594 Size: 219 1150 303 549 344 643 425 1135 Cuts at: 5594 6275 6466 826 Size: 681 191 1471 Fragments arranged by size: 1471 1150 1135 681 643 549 425 344 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 2732 4438 1872 Size: 860 1706 4545 Fragments arranged by size: 4545 1706 860 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2750 4383 5215 6116 6378 Size: 197 926 126 681 1633 832 901 262 Cuts at: 6378 820 Size: 1553 Fragments arranged by size: 1633 1553 926 901 832 681 262 197 126 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SspI AAT'ATT Cuts at: 5 52 2590 3190 3247 5035 5 Size: 47 2538 600 57 1788 2081 Fragments arranged by size: 2538 2081 1788 600 57 47 StuI AGG'CCT Cuts at: 1517 3970 1517 Size: 2453 4658 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 2385 2943 3090 3448 4550 Size: 552 939 345 36 558 147 358 1102 Cuts at: 4550 513 Size: 3074 Fragments arranged by size: 3074 1102 939 558 552 358 345 147 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2845 5138 5155 5308 5493 5938 325 Size: 1255 1265 2293 17 153 185 445 1498 Fragments arranged by size: 2293 1498 1265 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2961 5357 7069 Size: 274 80 33 51 1443 984 2396 1712 Cuts at: 7069 96 Size: 138 Fragments arranged by size: 2396 1712 1443 984 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2649 2681 7071 7103 2649 Size: 32 4390 32 2657 Fragments arranged by size: 4390 2657 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2983 1316 Size: 1667 5444 XbaI T'CTAG_A Cuts at: 4447 4447 Size: 7111 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2908 2908 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 2730 4436 1870 Size: 860 1706 4545 Fragments arranged by size: 4545 1706 860 XmnI GAAnn'nnTTC Cuts at: 2161 5240 2161 Size: 3079 4032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI