Restriction map for
pFN21AB9468
(Circular) (Six-base) MAPSORT of: pFN21AB9468.seq Check: 3186 from: 1 to: 11137
pFN21AB9468 11137 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 8947 278
Size: 53 83 186 936 476 6935 2468
Fragments arranged by size:
6935 2468 936 476 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7019 7269 8480 1937
Size: 5082 250 1211 4594
Fragments arranged by size:
5082 4594 1211 250
Acc65I G'GTAC_C
Cuts at: 8458 8458
Size: 11137
AclI AA'CG_TT
Cuts at: 1563 4112 6117 9264 9637 1563
Size: 2549 2005 3147 373 3063
Fragments arranged by size:
3147 3063 2549 2005 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3126 3188 3631 4118 4177 4890 5054 9200
Size: 1494 62 443 487 59 713 164 4146
Cuts at: 9200 10584 10852 1632
Size: 1384 268 1917
Fragments arranged by size:
4146 1917 1494 1384 713 487 443 268
164 62 59
AfeI AGC'GCT
Cuts at: 6861 6861
Size: 11137
AflII C'TTAA_G
Cuts at: 820 1017 2324 3731 820
Size: 197 1307 1407 8226
Fragments arranged by size:
8226 1407 1307 197
AflIII A'CryG_T
Cuts at: 1203 6169 10036 11013 1203
Size: 4966 3867 977 1327
Fragments arranged by size:
4966 3867 1327 977
AgeI A'CCGG_T
Cuts at: 9968 9968
Size: 11137
AhdI GACnn_n'nnGTC
Cuts at: 9866 9866
Size: 11137
AleI CACnn'nnGTG
Cuts at: 1166 2338 5156 7091 1166
Size: 1172 2818 1935 5212
Fragments arranged by size:
5212 2818 1935 1172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6548 6854 1184
Size: 5364 306 5467
Fragments arranged by size:
5467 5364 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4609 4641 4609
Size: 32 11105
AlwNI CAG_nnn'CTG
Cuts at: 2026 3440 4913 6335 6577 8212 8398 8880 10452
Size: 1414 1473 1422 242 1635 186 482 1572
Cuts at: 10452 2026
Size: 2711
Fragments arranged by size:
2711 1635 1572 1473 1422 1414 482 242
186
ApaLI G'TGCA_C
Cuts at: 5270 9194 10350 5270
Size: 3924 1156 6057
Fragments arranged by size:
6057 3924 1156
ApoI r'AATT_y
Cuts at: 1493 2842 3489 4021 4388 5210 6233 6338 6545
Size: 1349 647 532 367 822 1023 105 207
Cuts at: 6545 7042 8111 8446 8672 8824 10933 1493
Size: 497 1069 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 1069 1023 822 647 532
497 367 335 226 207 152 105
AseI AT'TA_AT
Cuts at: 160 1032 2463 3752 5765 9691 160
Size: 872 1431 1289 2013 3926 1606
Fragments arranged by size:
3926 2013 1606 1431 1289 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11137
AvaI C'yCGr_G
Cuts at: 1870 1943 2623 7461 8462 1870
Size: 73 680 4838 1001 4545
Fragments arranged by size:
4838 4545 1001 680 73
BamHI G'GATC_C
Cuts at: 4290 5236 8467 8834 4290
Size: 946 3231 367 6593
Fragments arranged by size:
6593 3231 946 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4520 5683 6186 8458 9913
Size: 325 217 627 2733 1163 503 2272 1455
Cuts at: 9913 618
Size: 1842
Fragments arranged by size:
2733 2272 1842 1455 1163 627 503 325
217
BanII G_rGCy'C
Cuts at: 1391 4329 5151 7584 7642 8456 1391
Size: 2938 822 2433 58 814 4072
Fragments arranged by size:
4072 2938 2433 822 814 58
BbeI G_GCGC'C
Cuts at: 6190 6190
Size: 11137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4183 4470 4724 5771 6573 6914 928
Size: 971 2284 287 254 1047 802 341 5151
Fragments arranged by size:
5151 2284 1047 971 802 341 287 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6296 6330 8142 8176 9328 9362 1903
Size: 34 4359 34 1812 34 1152 34 3678
Fragments arranged by size:
4359 3678 1812 1152 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9029 10239 9029
Size: 1210 9927
BclI T'GATC_A
Cuts at: 1548 2892 3781 6813 1548
Size: 1344 889 3032 5872
Fragments arranged by size:
5872 3032 1344 889
BfrBI ATG'CAT
Cuts at: 8148 8148
Size: 11137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9748 136
Size: 107 122 71 631 605 8076 1525
Fragments arranged by size:
8076 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2557 3361 5578 11132 2557
Size: 804 2217 5554 2562
Fragments arranged by size:
5554 2562 2217 804
BlpI GC'TnA_GC
Cuts at: 2632 3458 8554 2632
Size: 826 5096 5215
Fragments arranged by size:
5215 5096 826
Bme1580I G_kGCm'C
Cuts at: 1790 4312 5274 5531 5933 9198 10354 1790
Size: 2522 962 257 402 3265 1156 2573
Fragments arranged by size:
3265 2573 2522 1156 962 402 257
BmgBI CAC'GTC
Cuts at: 1318 6172 1318
Size: 4854 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4289 6230 7070
Size: 416 53 83 399 24 2872 1941 840
Cuts at: 7070 8868 9826 442
Size: 1798 958 1753
Fragments arranged by size:
2872 1941 1798 1753 958 840 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2225 3198 1056
Size: 1169 973 8995
Fragments arranged by size:
8995 1169 973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 7186 7273 7603 8438 5645
Size: 1541 87 330 835 8344
Fragments arranged by size:
8344 1541 835 330 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2532 4740 4814 5519 5790 6389 6677
Size: 1127 166 2208 74 705 271 599 288
Cuts at: 6677 7092 7199 7243 7391 7640 7682 8408 9797
Size: 415 107 44 148 249 42 726 1389
Cuts at: 9797 1239
Size: 2579
Fragments arranged by size:
2579 2208 1389 1127 726 705 599 415
288 271 249 166 148 107 74 44
42
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 11137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4662 9262 10127 10425 4662
Size: 4600 865 298 5374
Fragments arranged by size:
5374 4600 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6653 7261 9800 882
Size: 617 5154 608 2539 2219
Fragments arranged by size:
5154 2539 2219 617 608
BsaAI yAC'GTr
Cuts at: 493 1204 8928 493
Size: 711 7724 2702
Fragments arranged by size:
7724 2702 711
BsaBI GATnn'nnATC
Cuts at: 2755 6398 11131 2755
Size: 3643 4733 2761
Fragments arranged by size:
4733 3643 2761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 6187 8944 9326
Size: 53 83 186 936 476 4178 2757 382
Cuts at: 9326 9961 275
Size: 635 1451
Fragments arranged by size:
4178 2757 1451 936 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5950 9570 9968 10242 10389 10800 5950
Size: 3620 398 274 147 411 6287
Fragments arranged by size:
6287 3620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2055 5988 6018 1178
Size: 30 165 30 622 30 3933 30 6297
Fragments arranged by size:
6297 3933 622 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4094 4097 4910 5858 6239 7136 8221 1186
Size: 2908 3 813 948 381 897 1085 4102
Fragments arranged by size:
4102 2908 1085 948 897 813 381 3
BseYI C'CCAG_C
Cuts at: 2019 3921 7197 7205 7641 8025 10340 10836 2019
Size: 1902 3276 8 436 384 2315 496 2320
Fragments arranged by size:
3276 2320 2315 1902 496 436 384 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3887 4466 6246 6702 6980 7071 1163
Size: 573 2151 579 1780 456 278 91 5229
Fragments arranged by size:
5229 2151 1780 579 573 456 278 91
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8587 9348 10376 1540
Size: 275 186 6586 761 1028 2301
Fragments arranged by size:
6586 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2652 2925 4137 4329 5274 5437 6183 7584 9198
Size: 273 1212 192 945 163 746 1401 1614
Cuts at: 9198 9283 10354 2652
Size: 85 1071 3435
Fragments arranged by size:
3435 1614 1401 1212 1071 945 746 273
192 163 85
BsmI GAATG_Cn'
Cuts at: 5304 7347 8648 8741 5304
Size: 2043 1301 93 7700
Fragments arranged by size:
7700 2043 1301 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3076 4144 7424 3076
Size: 1068 3280 6789
Fragments arranged by size:
6789 3280 1068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2652 2925 4137 4312 4329 5151 5274
Size: 399 862 273 1212 175 17 822 123
Cuts at: 5274 5437 5531 5933 6183 7584 7642 8456 9198
Size: 163 94 402 250 1401 58 814 742
Cuts at: 9198 9283 10354 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1401 1212 1071 862 822 814 742
402 399 273 250 175 163 123 94
85 58 17
BspEI T'CCGG_A
Cuts at: 5950 10800 5950
Size: 4850 6287
BspHI T'CATG_A
Cuts at: 3519 9026 10756 3519
Size: 5507 1730 3900
Fragments arranged by size:
5507 3900 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7281 8492 844
Size: 6437 1211 3489
Fragments arranged by size:
6437 3489 1211
BsrBI CCG'CTC
Cuts at: 1394 1823 9024 1394
Size: 429 7201 3507
Fragments arranged by size:
7201 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 4707 7830 9632 9806 65
Size: 1183 1149 2310 3123 1802 174 1396
Fragments arranged by size:
3123 2310 1802 1396 1183 1149 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7868 9781 9968 1700
Size: 110 6058 1913 187 2869
Fragments arranged by size:
6058 2869 1913 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11137
BssHII G'CGCG_C
Cuts at: 1925 6736 6738 7143 1925
Size: 4811 2 405 5919
Fragments arranged by size:
5919 4811 405 2
BssSI C'ACGA_G
Cuts at: 2420 9197 10209 2420
Size: 6777 1012 3348
Fragments arranged by size:
6777 3348 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3052 5557 794
Size: 2258 2505 6374
Fragments arranged by size:
6374 2505 2258
BstBI TT'CG_AA
Cuts at: 9941 9941
Size: 11137
BstEII G'GTnAC_C
Cuts at: 1184 7165 1184
Size: 5981 5156
BstXI CCAn_nnnn'nTGG
Cuts at: 7252 7252
Size: 11137
BstYI r'GATC_y
Cuts at: 1972 2557 3361 4030 4290 5136 5236 5578 7063
Size: 585 804 669 260 846 100 342 1485
Cuts at: 7063 8467 8834 9219 9236 10688 10774 10786 11132
Size: 1404 367 385 17 1452 86 12 346
Cuts at: 11132 1972
Size: 1977
Fragments arranged by size:
1977 1485 1452 1404 846 804 669 585
385 367 346 342 260 100 86 17
12
BstZ17I GTA'TAC
Cuts at: 7020 7020
Size: 11137
Bsu36I CC'TnA_GG
Cuts at: 2103 8238 2103
Size: 6135 5002
BtgI C'CryG_G
Cuts at: 513 1065 2823 4350 5175 6696 7710 8169 513
Size: 552 1758 1527 825 1521 1014 459 3481
Fragments arranged by size:
3481 1758 1527 1521 1014 825 552 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2704 8134 487
Size: 683 56 1478 5430 3490
Fragments arranged by size:
5430 3490 1478 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 4248 4660 5455 6462 7086 8653 9447 9467
Size: 1703 412 795 1007 624 1567 794 20
Cuts at: 9467 2545
Size: 4215
Fragments arranged by size:
4215 1703 1567 1007 795 794 624 412
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3780 3815 529
Size: 35 3216 35 7851
Fragments arranged by size:
7851 3216 35 35
DraI TTT'AAA
Cuts at: 4158 5229 5458 6544 6633 8441 4158
Size: 1071 229 1086 89 1808 6854
Fragments arranged by size:
6854 1808 1086 1071 229 89
DraIII CAC_nnn'GTG
Cuts at: 5432 6224 7879 5432
Size: 792 1655 8690
Fragments arranged by size:
8690 1655 792
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10144 809
Size: 507 8828 1802
Fragments arranged by size:
8828 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 3495 4347
Size: 54 1425 50 275 18 282 1383 852
Cuts at: 4347 6951 7188 7870 8166 8584 9473 11102 8
Size: 2604 237 682 296 418 889 1629 43
Fragments arranged by size:
2604 1629 1425 1383 889 852 682 418
296 282 275 237 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8584 1537
Size: 275 6772 4090
Fragments arranged by size:
6772 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 3045 3204 3756 4317 5237 6117 6639
Size: 804 888 159 552 561 920 880 522
Cuts at: 6639 7572 8100 9067 1353
Size: 933 528 967 3423
Fragments arranged by size:
3423 967 933 920 888 880 804 561
552 528 522 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5870 8189 9724 10098 10244 387
Size: 1064 138 4281 2319 1535 374 146 1280
Fragments arranged by size:
4281 2319 1535 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4327 7582 4327
Size: 3255 7882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2532 3126 3188 3631 4118 4177
Size: 393 734 166 594 62 443 487 59
Cuts at: 4177 4740 4814 4890 5054 5519 5790 6389 6677
Size: 563 74 76 164 465 271 599 288
Cuts at: 6677 7092 7199 7243 7391 7640 7682 8408 9200
Size: 415 107 44 148 249 42 726 792
Cuts at: 9200 9797 10584 10852 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 792 787 734 726 599 597 594
563 487 465 443 415 393 288 271
268 249 166 164 148 107 76 74
62 59 44 42
EcoO109I rG'GnC_Cy
Cuts at: 4834 7624 7743 4834
Size: 2790 119 8228
Fragments arranged by size:
8228 2790 119
EcoRI G'AATT_C
Cuts at: 2842 3489 8446 8824 2842
Size: 647 4957 378 5155
Fragments arranged by size:
5155 4957 647 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2186 3154 3167 3193 3950 7987 2186
Size: 968 13 26 757 4037 5336
Fragments arranged by size:
5336 4037 968 757 26 13
FspI TGC'GCA
Cuts at: 9643 9643
Size: 11137
HaeII r_GCGC'y
Cuts at: 1678 2687 6190 6863 7187 10284 1678
Size: 1009 3503 673 324 3097 2531
Fragments arranged by size:
3503 3097 2531 1009 673 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2690 2722 2785
Size: 32 135 32 453 32 633 32 63
Cuts at: 2785 2817 3655 3687 3773 3805 3833 3865 4070
Size: 32 838 32 86 32 28 32 205
Cuts at: 4070 4102 4666 4698 4858 4890 5570 5602 5988
Size: 32 564 32 160 32 680 32 386
Cuts at: 5988 6020 6604 6636 8053 8085 9778 9810 9852
Size: 32 584 32 1417 32 1693 32 42
Cuts at: 9852 9884 1373
Size: 32 2626
Fragments arranged by size:
2626 1693 1417 838 680 633 584 564
453 386 205 160 135 86 63 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 5080 7806 8481 8727 669
Size: 988 281 3142 2726 675 246 3079
Fragments arranged by size:
3142 3079 2726 988 675 281 246
HindIII A'AGCT_T
Cuts at: 748 4220 5381 6388 6753 7028 748
Size: 3472 1161 1007 365 275 4857
Fragments arranged by size:
4857 3472 1161 1007 365 275
HpaI GTT'AAC
Cuts at: 8727 8727
Size: 11137
KasI G'GCGC_C
Cuts at: 6186 6186
Size: 11137
KpnI G_GTAC'C
Cuts at: 8462 8462
Size: 11137
MfeI C'AATT_G
Cuts at: 8736 8736
Size: 11137
MluI A'CGCG_T
Cuts at: 11013 11013
Size: 11137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 5581 7651 10251 10435 1305
Size: 948 3328 2070 2600 184 2007
Fragments arranged by size:
3328 2600 2070 2007 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3497 6953 7190 8168 10
Size: 54 1425 343 1665 3456 237 978 2979
Fragments arranged by size:
3456 2979 1665 1425 978 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2338 4565 5156 5910 6809 7091 9095
Size: 648 1172 2227 591 754 899 282 2004
Cuts at: 9095 9454 9613 518
Size: 359 159 2042
Fragments arranged by size:
2227 2042 2004 1172 899 754 648 591
359 282 159
MspA1I CmG'CkG
Cuts at: 1606 2026 3743 5156 8083 8553 9230 10378 10623
Size: 420 1717 1413 2927 470 677 1148 245
Cuts at: 10623 1606
Size: 2120
Fragments arranged by size:
2927 2120 1717 1413 1148 677 470 420
245
NaeI GCC'GGC
Cuts at: 7870 7870
Size: 11137
NarI GG'CG_CC
Cuts at: 6187 6187
Size: 11137
NcoI C'CATG_G
Cuts at: 513 1065 2823 5175 6696 7710 8169 513
Size: 552 1758 2352 1521 1014 459 3481
Fragments arranged by size:
3481 2352 1758 1521 1014 552 459
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11137
NgoMIV G'CCGG_C
Cuts at: 7868 7868
Size: 11137
NheI G'CTAG_C
Cuts at: 1052 2221 3194 1052
Size: 1169 973 8995
Fragments arranged by size:
8995 1169 973
NotI GC'GGCC_GC
Cuts at: 8584 8584
Size: 11137
NsiI A_TGCA'T
Cuts at: 8150 8150
Size: 11137
NspI r_CATG'y
Cuts at: 1132 1229 3055 8495 10040 1132
Size: 97 1826 5440 1545 2229
Fragments arranged by size:
5440 2229 1826 1545 97
PacI TTA_AT'TAA
Cuts at: 3756 3756
Size: 11137
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11137
PciI A'CATG_T
Cuts at: 10036 10036
Size: 11137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3190 5813 1108
Size: 2082 2623 6432
Fragments arranged by size:
6432 2623 2082
PfoI T'CCnGG_A
Cuts at: 5634 6174 6899 7617 8115 5634
Size: 540 725 718 498 8656
Fragments arranged by size:
8656 725 718 540 498
PmeI GTTT'AAAC
Cuts at: 8441 8441
Size: 11137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5834 5866 9206 9238 10719 10751 5834
Size: 32 3340 32 1481 32 6220
Fragments arranged by size:
6220 3340 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11137
PsiI TTA'TAA
Cuts at: 5261 8036 8707 5261
Size: 2775 671 7691
Fragments arranged by size:
7691 2775 671
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2524 2556 4788 4820 7985 8017 2524
Size: 32 2232 32 3165 32 5644
Fragments arranged by size:
5644 3165 2232 32 32 32
PstI C_TGCA'G
Cuts at: 830 2698 2931 4243 4655 4674 5649 6888 7299
Size: 1868 233 1312 412 19 975 1239 411
Cuts at: 7299 8489 830
Size: 1190 3478
Fragments arranged by size:
3478 1868 1312 1239 1190 975 412 411
233 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11137
PvuII CAG'CTG
Cuts at: 2026 5156 8083 2026
Size: 3130 2927 5080
Fragments arranged by size:
5080 3130 2927
SacI G_AGCT'C
Cuts at: 4329 7584 4329
Size: 3255 7882
SalI G'TCGA_C
Cuts at: 1936 8479 1936
Size: 6543 4594
SapI GCTCTTCn'nnn_
Cuts at: 4317 7572 4317
Size: 3255 7882
SbfI CC_TGCA'GG
Cuts at: 8489 8489
Size: 11137
ScaI AGT'ACT
Cuts at: 5067 6410 9385 11097 5067
Size: 1343 2975 1712 5107
Fragments arranged by size:
5107 2975 1712 1343
SexAI A'CCwGG_T
Cuts at: 7905 7905
Size: 11137
SfcI C'TryA_G
Cuts at: 826 1045 2694 2927 4239 4651 4670 4955 5645
Size: 219 1649 233 1312 412 19 285 690
Cuts at: 5645 6099 6494 6884 7295 8485 9620 10301 10492
Size: 454 395 390 411 1190 1135 681 191
Cuts at: 10492 826
Size: 1471
Fragments arranged by size:
1649 1471 1312 1190 1135 690 681 454
412 411 395 390 285 233 219 191
19
SfoI GGC'GCC
Cuts at: 6188 6188
Size: 11137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11137
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11137
SmaI CCC'GGG
Cuts at: 1872 2625 8464 1872
Size: 753 5839 4545
Fragments arranged by size:
5839 4545 753
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 3731 4677 9241 10142 10404
Size: 197 926 381 1407 946 4564 901 262
Cuts at: 10404 820
Size: 1553
Fragments arranged by size:
4564 1553 1407 946 926 901 381 262
197
SnaBI TAC'GTA
Cuts at: 493 8928 493
Size: 8435 2702
SpeI A'CTAG_T
Cuts at: 152 7382 152
Size: 7230 3907
SphI G_CATG'C
Cuts at: 1132 8495 1132
Size: 7363 3774
SspI AAT'ATT
Cuts at: 5 52 2433 5946 9061 5
Size: 47 2381 3513 3115 2081
Fragments arranged by size:
3513 3115 2381 2081 47
StuI AGG'CCT
Cuts at: 1517 6639 1517
Size: 5122 6015
StyI C'CwwG_G
Cuts at: 513 1065 2232 2823 2950 5175 5523 6696 7284
Size: 552 1167 591 127 2225 348 1173 588
Cuts at: 7284 7710 8169 8576 513
Size: 426 459 407 3074
Fragments arranged by size:
3074 2225 1173 1167 591 588 552 459
426 407 348 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 6212 9164 9181 9334 9519 9964
Size: 1255 2484 2148 2952 17 153 185 445
Cuts at: 9964 325
Size: 1498
Fragments arranged by size:
2952 2484 2148 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2150 5065 6408
Size: 274 80 33 51 1443 173 2915 1343
Cuts at: 6408 9383 11095 96
Size: 2975 1712 138
Fragments arranged by size:
2975 2915 1712 1443 1343 274 173 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7449 7481 11097 11129 7449
Size: 32 3616 32 7457
Fragments arranged by size:
7457 3616 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11137
XbaI T'CTAG_A
Cuts at: 2069 4573 5248 5581 8473 2069
Size: 2504 675 333 2892 4733
Fragments arranged by size:
4733 2892 2504 675 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4302 4838 4302
Size: 536 10601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11137
XmaI C'CCGG_G
Cuts at: 1870 2623 8462 1870
Size: 753 5839 4545
Fragments arranged by size:
5839 4545 753
XmnI GAAnn'nnTTC
Cuts at: 3276 4556 5370 5589 6444 7347 8322 9266 3276
Size: 1280 814 219 855 903 975 944 5147
Fragments arranged by size:
5147 1280 975 944 903 855 814 219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 8945 276
Size: 53 83 186 936 476 6935 2468
Fragments arranged by size:
6935 2468 936 476 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BsiWI EcoNI
EcoRV FseI FspAI NruI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SfiI SrfI SwaI