(Circular) (Six-base) MAPSORT of: pFN21AB9465.seq Check: 8330 from: 1 to: 7816 pFN21AB9465 7816 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2023 3276 3892 4139 2023 Size: 1253 616 247 5700 Fragments arranged by size: 5700 1253 616 247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5626 278 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5159 1937 Size: 3222 4594 Acc65I G'GTAC_C Cuts at: 5137 5137 Size: 7816 AclI AA'CG_TT Cuts at: 1563 5943 6316 1563 Size: 4380 373 3063 Fragments arranged by size: 4380 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3239 3635 4359 5879 7263 7531 1632 Size: 725 882 396 724 1520 1384 268 1917 Fragments arranged by size: 1917 1520 1384 882 725 724 396 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7619 AflIII A'CryG_T Cuts at: 1203 2625 6715 7692 1203 Size: 1422 4090 977 1327 Fragments arranged by size: 4090 1422 1327 977 AgeI A'CCGG_T Cuts at: 6647 6647 Size: 7816 AhdI GACnn_n'nnGTC Cuts at: 6545 6545 Size: 7816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2288 2293 2677 3645 1184 Size: 1104 5 384 968 5355 Fragments arranged by size: 5355 1104 968 384 5 AlwNI CAG_nnn'CTG Cuts at: 3268 3658 4039 5559 7131 3268 Size: 390 381 1520 1572 3953 Fragments arranged by size: 3953 1572 1520 390 381 ApaLI G'TGCA_C Cuts at: 5873 7029 5873 Size: 1156 6660 ApoI r'AATT_y Cuts at: 1493 3025 3296 4700 5125 5351 5503 7612 1493 Size: 1532 271 1404 425 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 1404 425 271 226 152 AseI AT'TA_AT Cuts at: 160 1032 2148 6370 160 Size: 872 1116 4222 1606 Fragments arranged by size: 4222 1606 1116 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 AvaI C'yCGr_G Cuts at: 1870 1943 4428 4821 5141 1870 Size: 73 2485 393 320 4545 Fragments arranged by size: 4545 2485 393 320 73 BamHI G'GATC_C Cuts at: 4470 5146 5513 4470 Size: 676 367 6773 Fragments arranged by size: 6773 676 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2201 4121 5137 6592 Size: 325 217 627 248 166 1920 1016 1455 Cuts at: 6592 618 Size: 1842 Fragments arranged by size: 1920 1842 1455 1016 627 325 248 217 166 BanII G_rGCy'C Cuts at: 1391 3324 4144 4305 5135 1391 Size: 1933 820 161 830 4072 Fragments arranged by size: 4072 1933 830 820 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3194 3590 4059 928 Size: 971 1295 396 469 4685 Fragments arranged by size: 4685 1295 971 469 396 BbvCI CC'TCA_GC Cuts at: 2908 5049 2908 Size: 2141 5675 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4094 4128 4802 4836 6007 6041 1903 Size: 34 2157 34 674 34 1171 34 3678 Fragments arranged by size: 3678 2157 1171 674 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5708 6918 5708 Size: 1210 6606 BclI T'GATC_A Cuts at: 1548 1548 Size: 7816 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4211 4634 6427 Size: 107 122 71 631 605 2539 423 1793 Cuts at: 6427 136 Size: 1525 Fragments arranged by size: 2539 1793 1525 631 605 423 122 107 71 BglII A'GATC_T Cuts at: 2815 3247 7811 2815 Size: 432 4564 2820 Fragments arranged by size: 4564 2820 432 BlpI GC'TnA_GC Cuts at: 2060 3354 4418 5233 2060 Size: 1294 1064 815 4643 Fragments arranged by size: 4643 1294 1064 815 Bme1580I G_kGCm'C Cuts at: 1790 2040 2204 2224 5877 7033 1790 Size: 250 164 20 3653 1156 2573 Fragments arranged by size: 3653 2573 1156 250 164 20 BmgBI CAC'GTC Cuts at: 1318 2536 3229 1318 Size: 1218 693 5905 Fragments arranged by size: 5905 1218 693 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2207 3679 3709 Size: 416 53 83 399 24 790 1472 30 Cuts at: 3709 5015 5547 6505 442 Size: 1306 532 958 1753 Fragments arranged by size: 1753 1472 1306 958 790 532 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 4743 1056 Size: 3687 4129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2544 2600 2815 3563 4037 4048 4241 Size: 869 436 56 215 748 474 11 193 Cuts at: 4241 4520 6476 1239 Size: 279 1956 2579 Fragments arranged by size: 2579 1956 869 748 474 436 279 215 193 56 11 Bpu10I CC'TnA_GC Cuts at: 2396 2908 3323 4149 4274 5049 2396 Size: 512 415 826 125 775 5163 Fragments arranged by size: 5163 826 775 512 415 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3372 3573 4259 4894 5941 6806 7104 3372 Size: 201 686 635 1047 865 298 4084 Fragments arranged by size: 4084 1047 865 686 635 298 201 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6479 882 Size: 617 4980 2219 Fragments arranged by size: 4980 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5607 493 Size: 711 4403 2702 Fragments arranged by size: 4403 2702 711 BsaBI GATnn'nnATC Cuts at: 7810 7810 Size: 7816 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3915 4107 5623 6005 Size: 53 83 186 936 2382 192 1516 382 Cuts at: 6005 6640 275 Size: 635 1451 Fragments arranged by size: 2382 1516 1451 936 635 382 192 186 83 53 BsaWI w'CCGG_w Cuts at: 6249 6647 6921 7068 7479 6249 Size: 398 274 147 411 6586 Fragments arranged by size: 6586 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3536 3566 3962 3992 4199 Size: 30 165 30 2133 30 396 30 207 Cuts at: 4199 4229 4641 4671 1178 Size: 30 412 30 4323 Fragments arranged by size: 4323 2133 412 396 207 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 3782 3930 3965 4313 4349 4424 4721 Size: 1114 1482 148 35 348 36 75 297 Cuts at: 4721 1186 Size: 4281 Fragments arranged by size: 4281 1482 1114 348 297 148 75 36 35 BseYI C'CCAG_C Cuts at: 2081 2755 2871 3956 4339 4644 4749 4803 5055 Size: 674 116 1085 383 305 105 54 252 Cuts at: 5055 7019 7515 2081 Size: 1964 496 2382 Fragments arranged by size: 2382 1964 1085 674 496 383 305 252 116 105 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2440 1163 Size: 573 704 6539 Fragments arranged by size: 6539 704 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3285 5266 6027 7055 1540 Size: 275 186 1284 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3324 4305 4515 5877 5962 7033 2277 Size: 1047 981 210 1362 85 1071 3060 Fragments arranged by size: 3060 1362 1071 1047 981 210 85 BsmI GAATG_Cn' Cuts at: 3475 4249 5327 5420 3475 Size: 774 1078 93 5871 Fragments arranged by size: 5871 1078 774 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2204 2224 2277 3324 4144 4305 Size: 399 250 164 20 53 1047 820 161 Cuts at: 4305 4515 5135 5877 5962 7033 1391 Size: 210 620 742 85 1071 2174 Fragments arranged by size: 2174 1071 1047 820 742 620 399 250 210 164 161 85 53 20 BspEI T'CCGG_A Cuts at: 7479 7479 Size: 7816 BspHI T'CATG_A Cuts at: 4726 5705 7435 4726 Size: 979 1730 5107 Fragments arranged by size: 5107 1730 979 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2499 2847 3276 3465 3892 4139 5171 Size: 1179 476 348 429 189 427 247 1032 Cuts at: 5171 844 Size: 3489 Fragments arranged by size: 3489 1179 1032 476 429 427 348 247 189 BsrBI CCG'CTC Cuts at: 1394 1823 2116 2634 2829 3733 4557 5703 1394 Size: 429 293 518 195 904 824 1146 3507 Fragments arranged by size: 3507 1146 904 824 518 429 293 195 BsrDI GCAATG_nn' Cuts at: 65 1248 4110 6311 6485 65 Size: 1183 2862 2201 174 1396 Fragments arranged by size: 2862 2201 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 4844 6460 6647 1700 Size: 110 254 2780 1616 187 2869 Fragments arranged by size: 2869 2780 1616 254 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7816 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7816 BssSI C'ACGA_G Cuts at: 5876 6888 5876 Size: 1012 6804 BstAPI GCAn_nnn'nTGC Cuts at: 794 3268 794 Size: 2474 5342 BstBI TT'CG_AA Cuts at: 6620 6620 Size: 7816 BstEII G'GTnAC_C Cuts at: 1184 3478 5101 1184 Size: 2294 1623 3899 Fragments arranged by size: 3899 2294 1623 BstXI CCAn_nnnn'nTGG Cuts at: 4318 4639 4660 4318 Size: 321 21 7474 Fragments arranged by size: 7474 321 21 BstYI r'GATC_y Cuts at: 1972 2815 2865 3247 4470 5146 5513 5898 5915 Size: 843 50 382 1223 676 367 385 17 Cuts at: 5915 7367 7453 7465 7811 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1223 843 676 385 382 367 346 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3495 3981 4395 513 Size: 552 939 1491 486 414 3934 Fragments arranged by size: 3934 1491 939 552 486 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7077 Fragments arranged by size: 7077 683 56 BtsI GCAGTG_nn' Cuts at: 2045 2277 2666 3079 4839 5087 5332 6126 6146 Size: 232 389 413 1760 248 245 794 20 Cuts at: 6146 2045 Size: 3715 Fragments arranged by size: 3715 1760 794 413 389 248 245 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7816 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7781 DraI TTT'AAA Cuts at: 5120 5120 Size: 7816 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2233 2720 4445 6823 809 Size: 507 917 487 1725 2378 1802 Fragments arranged by size: 2378 1802 1725 917 507 487 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2410 3252 Size: 54 1425 50 275 18 186 394 842 Cuts at: 3252 3282 3498 3819 4368 4398 5263 6152 7781 Size: 30 216 321 549 30 865 889 1629 Cuts at: 7781 8 Size: 43 Fragments arranged by size: 1629 1425 889 865 842 549 394 321 275 216 186 54 50 43 30 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3282 5263 1537 Size: 275 1470 1981 4090 Fragments arranged by size: 4090 1981 1470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 2738 3789 4221 4881 5746 1353 Size: 1250 135 1051 432 660 865 3423 Fragments arranged by size: 3423 1250 1051 865 660 432 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 6403 6777 6923 387 Size: 1064 138 522 4292 374 146 1280 Fragments arranged by size: 4292 1280 1064 522 374 146 138 EcoICRI GAG'CTC Cuts at: 3322 4303 3322 Size: 981 6835 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2357 2544 2600 2815 3239 3563 Size: 393 476 249 187 56 215 424 324 Cuts at: 3563 3635 4037 4048 4241 4359 4520 5879 6476 Size: 72 402 11 193 118 161 1359 597 Cuts at: 6476 7263 7531 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1359 787 597 476 424 402 393 324 268 249 215 193 187 161 118 72 56 11 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2987 4496 2399 Size: 588 1509 5719 Fragments arranged by size: 5719 1509 588 EcoO109I rG'GnC_Cy Cuts at: 4345 4531 4345 Size: 186 7630 EcoRI G'AATT_C Cuts at: 3296 5125 5503 3296 Size: 1829 378 5609 Fragments arranged by size: 5609 1829 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2749 3145 2749 Size: 396 7420 FspI TGC'GCA Cuts at: 6322 6322 Size: 7816 HaeII r_GCGC'y Cuts at: 1678 2082 6963 1678 Size: 404 4881 2531 Fragments arranged by size: 4881 2531 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2945 2977 3761 3793 4021 Size: 32 135 32 1373 32 784 32 228 Cuts at: 4021 4053 4308 4340 4641 4673 4936 4968 6457 Size: 32 255 32 301 32 263 32 1489 Cuts at: 6457 6489 6531 6563 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1489 1373 784 301 263 255 228 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2245 2556 5160 5406 669 Size: 988 281 307 311 2604 246 3079 Fragments arranged by size: 3079 2604 988 311 307 281 246 HindIII A'AGCT_T Cuts at: 748 2726 748 Size: 1978 5838 HpaI GTT'AAC Cuts at: 2556 5406 2556 Size: 2850 4966 KpnI G_GTAC'C Cuts at: 5141 5141 Size: 7816 MfeI C'AATT_G Cuts at: 5415 5415 Size: 7816 MluI A'CGCG_T Cuts at: 7692 7692 Size: 7816 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3534 6930 7114 1305 Size: 2229 3396 184 2007 Fragments arranged by size: 3396 2229 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 2412 3500 3821 10 Size: 54 1425 343 186 394 1088 321 4005 Fragments arranged by size: 4005 1425 1088 394 343 321 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2709 3005 4259 4658 5774 6133 6292 Size: 648 1543 296 1254 399 1116 359 159 Cuts at: 6292 518 Size: 2042 Fragments arranged by size: 2042 1543 1254 1116 648 399 359 296 159 MspA1I CmG'CkG Cuts at: 1606 2416 2512 2677 2759 2875 3202 3217 3334 Size: 810 96 165 82 116 327 15 117 Cuts at: 3334 3358 3580 3598 3951 4002 4379 4673 4807 Size: 24 222 18 353 51 377 294 134 Cuts at: 4807 5232 5909 7057 7302 1606 Size: 425 677 1148 245 2120 Fragments arranged by size: 2120 1148 810 677 425 377 353 327 294 245 222 165 134 117 116 96 82 51 24 18 15 NaeI GCC'GGC Cuts at: 2066 4846 2066 Size: 2780 5036 NcoI C'CATG_G Cuts at: 513 1065 2004 3495 513 Size: 552 939 1491 4834 Fragments arranged by size: 4834 1491 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7816 NgoMIV G'CCGG_C Cuts at: 2064 4844 2064 Size: 2780 5036 NheI G'CTAG_C Cuts at: 1052 4739 1052 Size: 3687 4129 NotI GC'GGCC_GC Cuts at: 5263 5263 Size: 7816 NspI r_CATG'y Cuts at: 1132 1229 2629 5174 6719 1132 Size: 97 1400 2545 1545 2229 Fragments arranged by size: 2545 2229 1545 1400 97 PasI CC'CwG_GG Cuts at: 1348 4311 1348 Size: 2963 4853 PciI A'CATG_T Cuts at: 2625 6715 2625 Size: 4090 3726 PflMI CCAn_nnn'nTGG Cuts at: 1108 4039 4756 4945 1108 Size: 2931 717 189 3979 Fragments arranged by size: 3979 2931 717 189 PfoI T'CCnGG_A Cuts at: 3884 3884 Size: 7816 PmeI GTTT'AAAC Cuts at: 5120 5120 Size: 7816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2580 2612 3927 3959 5885 5917 7398 7430 2580 Size: 32 1315 32 1926 32 1481 32 2966 Fragments arranged by size: 2966 1926 1481 1315 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4345 4531 4345 Size: 186 7630 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7816 PsiI TTA'TAA Cuts at: 5386 5386 Size: 7816 PstI C_TGCA'G Cuts at: 830 2496 2702 2844 2953 3216 3801 3967 4812 Size: 1666 206 142 109 263 585 166 845 Cuts at: 4812 5168 830 Size: 356 3478 Fragments arranged by size: 3478 1666 845 585 356 263 206 166 142 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7816 PvuII CAG'CTG Cuts at: 2416 2512 2759 2875 3217 3598 3951 4002 4379 Size: 96 247 116 342 381 353 51 377 Cuts at: 4379 4673 4807 2416 Size: 294 134 5425 Fragments arranged by size: 5425 381 377 353 342 294 247 134 116 96 51 SacI G_AGCT'C Cuts at: 3324 4305 3324 Size: 981 6835 SalI G'TCGA_C Cuts at: 1936 5158 1936 Size: 3222 4594 SapI GCTCTTCn'nnn_ Cuts at: 3789 3789 Size: 7816 SbfI CC_TGCA'GG Cuts at: 5168 5168 Size: 7816 ScaI AGT'ACT Cuts at: 2846 6064 7776 2846 Size: 3218 1712 2886 Fragments arranged by size: 3218 2886 1712 SexAI A'CCwGG_T Cuts at: 4347 4347 Size: 7816 SfcI C'TryA_G Cuts at: 826 1045 2492 2698 2840 2949 3212 3797 3963 Size: 219 1447 206 142 109 263 585 166 Cuts at: 3963 4808 5164 6299 6980 7171 826 Size: 845 356 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 845 681 585 356 263 219 206 191 166 142 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7816 SmaI CCC'GGG Cuts at: 1872 5143 1872 Size: 3271 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3387 3588 4238 4909 5920 6821 Size: 197 926 1444 201 650 671 1011 901 Cuts at: 6821 7083 820 Size: 262 1553 Fragments arranged by size: 1553 1444 1011 926 901 671 650 262 201 197 SnaBI TAC'GTA Cuts at: 493 5607 493 Size: 5114 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7816 SphI G_CATG'C Cuts at: 1132 5174 1132 Size: 4042 3774 SspI AAT'ATT Cuts at: 5 52 5740 5 Size: 47 5688 2081 Fragments arranged by size: 5688 2081 47 StuI AGG'CCT Cuts at: 1517 2957 4175 4817 1517 Size: 1440 1218 642 4516 Fragments arranged by size: 4516 1440 1218 642 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3495 4474 4632 4767 5255 Size: 552 939 48 1443 979 158 135 488 Cuts at: 5255 513 Size: 3074 Fragments arranged by size: 3074 1443 979 939 552 488 158 135 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2905 4184 5843 5860 6013 6198 Size: 1255 639 686 1279 1659 17 153 185 Cuts at: 6198 6643 325 Size: 445 1498 Fragments arranged by size: 1659 1498 1279 1255 686 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 3426 6062 Size: 274 80 33 51 1443 867 582 2636 Cuts at: 6062 7774 96 Size: 1712 138 Fragments arranged by size: 2636 1712 1443 867 582 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2455 2487 2880 2912 3824 3856 3962 3994 7776 Size: 32 393 32 912 32 106 32 3782 Cuts at: 7776 7808 2455 Size: 32 2463 Fragments arranged by size: 3782 2463 912 393 106 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7816 XbaI T'CTAG_A Cuts at: 3607 5152 3607 Size: 1545 6271 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 4084 4660 2011 Size: 2073 576 5167 Fragments arranged by size: 5167 2073 576 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7816 XmaI C'CCGG_G Cuts at: 1870 5141 1870 Size: 3271 4545 XmnI GAAnn'nnTTC Cuts at: 5945 5945 Size: 7816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5624 276 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI