Restriction map for
pFN21AB9462
(Linear) (Six-base) MAPSORT of: KIBB9462.seq Check: 592 from: 1 to: 2736
KIBB9462.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 331 1502 1960 2736
Size: 331 1171 458 776
Fragments arranged by size:
1171 776 458 331
AccI GT'mk_AC
Cuts at: 0 2525 2736
Size: 2525 211
Acc65I G'GTAC_C
Cuts at: 0 1640 2736
Size: 1640 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 2094 2736
Size: 452 1642 642
Fragments arranged by size:
1642 642 452
AfeI AGC'GCT
Cuts at: 0 2056 2690 2736
Size: 2056 634 46
Fragments arranged by size:
2056 634 46
AflII C'TTAA_G
Cuts at: 0 208 1339 2736
Size: 208 1131 1397
Fragments arranged by size:
1397 1131 208
AflIII A'CryG_T
Cuts at: 0 444 605 1675 2736
Size: 444 161 1070 1061
Fragments arranged by size:
1070 1061 444 161
AleI CACnn'nnGTG
Cuts at: 0 1825 2736
Size: 1825 911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1351 2736
Size: 1351 1385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 2736
Size: 646 32 2058
Fragments arranged by size:
2058 646 32
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1542 2133 2736
Size: 357 1185 591 603
Fragments arranged by size:
1185 603 591 357
ApaI G_GGCC'C
Cuts at: 0 36 2736
Size: 36 2700
ApaLI G'TGCA_C
Cuts at: 0 1161 2298 2736
Size: 1161 1137 438
Fragments arranged by size:
1161 1137 438
ApoI r'AATT_y
Cuts at: 0 120 2736
Size: 120 2616
AvaI C'yCGr_G
Cuts at: 0 346 1860 2332 2736
Size: 346 1514 472 404
Fragments arranged by size:
1514 472 404 346
BanI G'GyrC_C
Cuts at: 0 304 594 1327 1640 1948 2419 2736
Size: 304 290 733 313 308 471 317
Fragments arranged by size:
733 471 317 313 308 304 290
BanII G_rGCy'C
Cuts at: 0 36 184 478 779 931 946 1301 1753
Size: 36 148 294 301 152 15 355 452
Cuts at: 1753 1940 2666 2736
Size: 187 726 70
Fragments arranged by size:
726 452 355 301 294 187 152 148
70 36 15
BbeI G_GCGC'C
Cuts at: 0 308 2736
Size: 308 2428
BbsI GAAGACnn'nnnn_
Cuts at: 0 1597 2736
Size: 1597 1139
BbvCI CC'TCA_GC
Cuts at: 0 58 983 2736
Size: 58 925 1753
Fragments arranged by size:
1753 925 58
BclI T'GATC_A
Cuts at: 0 1817 2736
Size: 1817 919
BglI GCCn_nnn'nGGC
Cuts at: 0 68 2270 2736
Size: 68 2202 466
Fragments arranged by size:
2202 466 68
BlpI GC'TnA_GC
Cuts at: 0 1120 1934 2736
Size: 1120 814 802
Fragments arranged by size:
1120 814 802
Bme1580I G_kGCm'C
Cuts at: 0 36 599 645 1165 2070 2302 2333 2561
Size: 36 563 46 520 905 232 31 228
Cuts at: 2561 2736
Size: 175
Fragments arranged by size:
905 563 520 232 228 175 46 36
31
BmgBI CAC'GTC
Cuts at: 0 1152 1676 2736
Size: 1152 524 1060
Fragments arranged by size:
1152 1060 524
BmrI ACTGGGnnnn_n'
Cuts at: 0 160 747 2736
Size: 160 587 1989
Fragments arranged by size:
1989 587 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1767 2736
Size: 1767 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 312 838 901 991 1029 2736
Size: 256 56 526 63 90 38 1707
Fragments arranged by size:
1707 526 256 90 63 56 38
Bpu10I CC'TnA_GC
Cuts at: 0 58 983 2736
Size: 58 925 1753
Fragments arranged by size:
1753 925 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1120 1762 2215 2698 2736
Size: 631 489 642 453 483 38
Fragments arranged by size:
642 631 489 483 453 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 2329 2475 2736
Size: 486 1843 146 261
Fragments arranged by size:
1843 486 261 146
BsaAI yAC'GTr
Cuts at: 0 608 1881 2297 2736
Size: 608 1273 416 439
Fragments arranged by size:
1273 608 439 416
BsaBI GATnn'nnATC
Cuts at: 0 1990 2736
Size: 1990 746
BsaHI Gr'CG_yC
Cuts at: 0 305 2736
Size: 305 2431
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 568 598 2736
Size: 310 30 228 30 2138
Fragments arranged by size:
2138 310 228 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 988 2736
Size: 46 942 1748
Fragments arranged by size:
1748 942 46
BseYI C'CCAG_C
Cuts at: 0 300 938 1331 2133 2268 2685 2736
Size: 300 638 393 802 135 417 51
Fragments arranged by size:
802 638 417 393 300 135 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 949 1237 1243 1480 2736
Size: 388 561 288 6 237 1256
Fragments arranged by size:
1256 561 388 288 237 6
BsiEI CG_ry'CG
Cuts at: 0 1035 1290 2435 2736
Size: 1035 255 1145 301
Fragments arranged by size:
1145 1035 301 255
BsiHKAI G_wGCw'C
Cuts at: 0 478 1165 2231 2302 2666 2736
Size: 478 687 1066 71 364 70
Fragments arranged by size:
1066 687 478 364 71 70
BsiWI C'GTAC_G
Cuts at: 0 1020 2736
Size: 1020 1716
BsmI GAATG_Cn'
Cuts at: 0 311 2510 2736
Size: 311 2199 226
Fragments arranged by size:
2199 311 226
Bsp1286I G_dGCh'C
Cuts at: 0 36 184 478 599 645 779 931 946
Size: 36 148 294 121 46 134 152 15
Cuts at: 946 1165 1301 1753 1940 2070 2231 2302 2333
Size: 219 136 452 187 130 161 71 31
Cuts at: 2333 2561 2666 2736
Size: 228 105 70
Fragments arranged by size:
452 294 228 219 187 161 152 148
136 134 130 121 105 71 70 46
36 31 15
BspHI T'CATG_A
Cuts at: 0 1925 2736
Size: 1925 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 331 1502 1960 2736
Size: 290 41 1171 458 776
Fragments arranged by size:
1171 776 458 290 41
BsrBI CCG'CTC
Cuts at: 0 698 1512 1532 1550 1584 2736
Size: 698 814 20 18 34 1152
Fragments arranged by size:
1152 814 698 34 20 18
BsrFI r'CCGG_y
Cuts at: 0 230 1067 1718 2736
Size: 230 837 651 1018
Fragments arranged by size:
1018 837 651 230
BsrGI T'GTAC_A
Cuts at: 0 2372 2444 2736
Size: 2372 72 292
Fragments arranged by size:
2372 292 72
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1266 1542 1610 1715 2556 2736
Size: 1266 276 68 105 841 180
Fragments arranged by size:
1266 841 276 180 105 68
BstBI TT'CG_AA
Cuts at: 0 826 2736
Size: 826 1910
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 2736
Size: 492 2244
BtgI C'CryG_G
Cuts at: 0 623 769 1244 1784 2736
Size: 623 146 475 540 952
Fragments arranged by size:
952 623 540 475 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 732 1356 1779 2736
Size: 732 624 423 957
Fragments arranged by size:
957 732 624 423
BtsI GCAGTG_nn'
Cuts at: 0 417 1110 1620 2736
Size: 417 693 510 1116
Fragments arranged by size:
1116 693 510 417
ClaI AT'CG_AT
Cuts at: 0 1229 2736
Size: 1229 1507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 180 215 2736
Size: 180 35 2521
Fragments arranged by size:
2521 180 35
DraIII CAC_nnn'GTG
Cuts at: 0 2175 2736
Size: 2175 561
EaeI y'GGCC_r
Cuts at: 0 174 494 1287 1522 1781 2099 2432 2597
Size: 174 320 793 235 259 318 333 165
Cuts at: 2597 2693 2736
Size: 96 43
Fragments arranged by size:
793 333 320 318 259 235 174 165
96 43
EagI C'GGCC_G
Cuts at: 0 1287 2432 2736
Size: 1287 1145 304
Fragments arranged by size:
1287 1145 304
EarI CTCTTCn'nnn_
Cuts at: 0 4 517 1780 2542 2736
Size: 4 513 1263 762 194
Fragments arranged by size:
1263 762 513 194 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 796 2736
Size: 796 1940
EcoICRI GAG'CTC
Cuts at: 0 476 2664 2736
Size: 476 2188 72
Fragments arranged by size:
2188 476 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 312 452 838 901 991 1029 2094
Size: 256 56 140 386 63 90 38 1065
Cuts at: 2094 2736
Size: 642
Fragments arranged by size:
1065 642 386 256 140 90 63 56
38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 327 973 1138 1634 2736
Size: 327 646 165 496 1102
Fragments arranged by size:
1102 646 496 327 165
EcoO109I rG'GnC_Cy
Cuts at: 0 2682 2736
Size: 2682 54
EcoRI G'AATT_C
Cuts at: 0 120 2736
Size: 120 2616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 2649 2736
Size: 33 2616 87
Fragments arranged by size:
2616 87 33
FspI TGC'GCA
Cuts at: 0 1447 2736
Size: 1447 1289
HaeII r_GCGC'y
Cuts at: 0 308 376 550 1422 2058 2489 2692 2736
Size: 308 68 174 872 636 431 203 44
Fragments arranged by size:
872 636 431 308 203 174 68 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 568 600 2641 2673 2736
Size: 310 32 226 32 2041 32 63
Fragments arranged by size:
2041 310 226 63 32 32 32
HincII GTy'rAC
Cuts at: 0 1359 2736
Size: 1359 1377
HindIII A'AGCT_T
Cuts at: 0 1738 2736
Size: 1738 998
KasI G'GCGC_C
Cuts at: 0 304 2736
Size: 304 2432
KpnI G_GTAC'C
Cuts at: 0 1644 2736
Size: 1644 1092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2633 2736
Size: 2633 103
MscI TGG'CCA
Cuts at: 0 496 2101 2695 2736
Size: 496 1605 594 41
Fragments arranged by size:
1605 594 496 41
MslI CAynn'nnrTG
Cuts at: 0 490 1825 2143 2736
Size: 490 1335 318 593
Fragments arranged by size:
1335 593 490 318
MspA1I CmG'CkG
Cuts at: 0 404 938 1335 1443 1542 2097 2130 2196
Size: 404 534 397 108 99 555 33 66
Cuts at: 2196 2736
Size: 540
Fragments arranged by size:
555 540 534 404 397 108 99 66
33
NaeI GCC'GGC
Cuts at: 0 1720 2736
Size: 1720 1016
NarI GG'CG_CC
Cuts at: 0 305 2736
Size: 305 2431
NcoI C'CATG_G
Cuts at: 0 623 1244 2736
Size: 623 621 1492
Fragments arranged by size:
1492 623 621
NgoMIV G'CCGG_C
Cuts at: 0 1718 2736
Size: 1718 1018
NspI r_CATG'y
Cuts at: 0 1608 1692 2283 2517 2736
Size: 1608 84 591 234 219
Fragments arranged by size:
1608 591 234 219 84
PasI CC'CwG_GG
Cuts at: 0 90 1757 2499 2736
Size: 90 1667 742 237
Fragments arranged by size:
1667 742 237 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 624 2175 2736
Size: 234 390 1551 561
Fragments arranged by size:
1551 561 390 234
PfoI T'CCnGG_A
Cuts at: 0 815 1480 2186 2736
Size: 815 665 706 550
Fragments arranged by size:
815 706 665 550
PmlI CAC'GTG
Cuts at: 0 2297 2736
Size: 2297 439
PshAI GACnn'nnGTC
Cuts at: 0 1280 2710 2736
Size: 1280 1430 26
Fragments arranged by size:
1430 1280 26
PspOMI G'GGCC_C
Cuts at: 0 32 2736
Size: 32 2704
PspXI vC'TCGA_Gb
Cuts at: 0 346 2736
Size: 346 2390
PstI C_TGCA'G
Cuts at: 0 1561 2129 2736
Size: 1561 568 607
Fragments arranged by size:
1561 607 568
PvuII CAG'CTG
Cuts at: 0 938 1335 1443 1542 2097 2130 2736
Size: 938 397 108 99 555 33 606
Fragments arranged by size:
938 606 555 397 108 99 33
SacI G_AGCT'C
Cuts at: 0 478 2666 2736
Size: 478 2188 70
Fragments arranged by size:
2188 478 70
SexAI A'CCwGG_T
Cuts at: 0 1649 2736
Size: 1649 1087
SfcI C'TryA_G
Cuts at: 0 1319 1557 2125 2274 2736
Size: 1319 238 568 149 462
Fragments arranged by size:
1319 568 462 238 149
SfoI GGC'GCC
Cuts at: 0 306 2736
Size: 306 2430
SmlI C'TyrA_G
Cuts at: 0 208 346 646 1135 1339 1741 2230 2677
Size: 208 138 300 489 204 402 489 447
Cuts at: 2677 2736
Size: 59
Fragments arranged by size:
489 489 447 402 300 208 204 138
59
SphI G_CATG'C
Cuts at: 0 1608 2283 2736
Size: 1608 675 453
Fragments arranged by size:
1608 675 453
StuI AGG'CCT
Cuts at: 0 158 709 1858 2736
Size: 158 551 1149 878
Fragments arranged by size:
1149 878 551 158
StyI C'CwwG_G
Cuts at: 0 623 779 1244 1944 2736
Size: 623 156 465 700 792
Fragments arranged by size:
792 700 623 465 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 438 442 468 1345 2049 2736
Size: 352 86 4 26 877 704 687
Fragments arranged by size:
877 704 687 352 86 26 4
TatI w'GTAC_w
Cuts at: 0 1343 1463 2372 2444 2736
Size: 1343 120 909 72 292
Fragments arranged by size:
1343 909 292 120 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 2736
Size: 106 32 2598
Fragments arranged by size:
2598 106 32
Tth111I GACn'n_nGTC
Cuts at: 0 634 2736
Size: 634 2102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1809 2736
Size: 1053 756 927
Fragments arranged by size:
1053 927 756
XhoI C'TCGA_G
Cuts at: 0 346 2736
Size: 346 2390
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BstAPI BstBI BstXI BtgI BtgZI BtsI ClaI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI PstI PvuII SacI SexAI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI
Enzymes that do not cut:
AatII AclI AgeI AhdI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BfrBI BglII BmtI BsaWI
BsmBI BspEI BsrDI BssHII BssSI BstEII BstYI BstZ17I
Bsu36I DraI DrdI EcoRV FseI FspAI HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PpuMI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI
XmnI ZraI