(Circular) (Six-base) MAPSORT of: pFN21AB9461.seq Check: 6367 from: 1 to: 6379 pFN21AB9461 6379 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2975 3186 2975 Size: 211 6168 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 2642 4442 5826 6094 1632 Size: 917 93 1800 1384 268 1917 Fragments arranged by size: 1917 1800 1384 917 268 93 AflII C'TTAA_G Cuts at: 820 1017 3185 820 Size: 197 2168 4014 Fragments arranged by size: 4014 2168 197 AflIII A'CryG_T Cuts at: 1203 2274 5278 6255 1203 Size: 1071 3004 977 1327 Fragments arranged by size: 3004 1327 1071 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 3380 1166 Size: 2214 4165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6379 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3276 3308 3276 Size: 32 6347 AlwNI CAG_nnn'CTG Cuts at: 2638 4122 5694 2638 Size: 1484 1572 3323 Fragments arranged by size: 3323 1572 1484 ApaLI G'TGCA_C Cuts at: 3469 4436 5592 3469 Size: 967 1156 4256 Fragments arranged by size: 4256 1156 967 ApoI r'AATT_y Cuts at: 1493 2370 2675 2705 2879 3239 3688 3914 4066 Size: 877 305 30 174 360 449 226 152 Cuts at: 4066 6175 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 877 449 360 305 226 174 152 30 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2924 3704 1870 Size: 73 981 780 4545 Fragments arranged by size: 4545 981 780 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3599 3632 3599 Size: 33 6346 BamHI G'GATC_C Cuts at: 2169 3709 4076 2169 Size: 1540 367 4472 Fragments arranged by size: 4472 1540 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2699 3700 5155 618 Size: 325 217 627 912 1001 1455 1842 Fragments arranged by size: 1842 1455 1001 912 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 3698 1391 Size: 681 1626 4072 Fragments arranged by size: 4072 1626 681 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2681 2715 4570 4604 1903 Size: 34 744 34 1855 34 3678 Fragments arranged by size: 3678 1855 744 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2434 4271 5481 2434 Size: 1837 1210 3332 Fragments arranged by size: 3332 1837 1210 BclI T'GATC_A Cuts at: 1548 2511 1548 Size: 963 5416 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4990 136 Size: 107 122 71 631 605 3318 1525 Fragments arranged by size: 3318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3006 6374 3006 Size: 3368 3011 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 3473 4440 5596 1790 Size: 1683 967 1156 2573 Fragments arranged by size: 2573 1683 1156 967 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4110 5068 442 Size: 416 53 83 399 24 2693 958 1753 Fragments arranged by size: 2693 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3429 1056 Size: 2373 4006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2244 2558 2680 3516 5039 1239 Size: 1005 314 122 836 1523 2579 Fragments arranged by size: 2579 1523 1005 836 314 122 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 6379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4504 5369 5667 2708 Size: 1796 865 298 3420 Fragments arranged by size: 3420 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 2734 5042 882 Size: 617 855 380 2308 2219 Fragments arranged by size: 2308 2219 855 617 380 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3486 4812 5210 5484 5631 6042 3486 Size: 1326 398 274 147 411 3823 Fragments arranged by size: 3823 1326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6154 Fragments arranged by size: 6154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2096 1186 Size: 907 3 5469 Fragments arranged by size: 5469 907 3 BseYI C'CCAG_C Cuts at: 3346 3511 5582 6078 3346 Size: 165 2071 496 3647 Fragments arranged by size: 3647 2071 496 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3612 1163 Size: 573 1876 3930 Fragments arranged by size: 3930 1876 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 3473 4440 4525 5596 2072 Size: 1401 967 85 1071 2855 Fragments arranged by size: 2855 1401 1071 967 85 BsmI GAATG_Cn' Cuts at: 3890 3983 3890 Size: 93 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 3473 3698 4440 4525 5596 1391 Size: 399 282 1401 225 742 85 1071 2174 Fragments arranged by size: 2174 1401 1071 742 399 282 225 85 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 3186 3264 3734 844 Size: 2131 211 78 470 3489 Fragments arranged by size: 3489 2131 470 211 78 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 4874 5048 65 Size: 1183 1362 2264 174 1396 Fragments arranged by size: 2264 1396 1362 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3246 5023 5210 1700 Size: 110 1436 1777 187 2869 Fragments arranged by size: 2869 1777 1436 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 3576 96 Size: 2181 1299 2899 Fragments arranged by size: 2899 2181 1299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6379 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstXI CCAn_nnnn'nTGG Cuts at: 2710 2710 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2169 2348 3006 3709 4076 4461 4478 5930 Size: 197 179 658 703 367 385 17 1452 Cuts at: 5930 6016 6028 6374 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 703 658 385 367 346 197 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3081 513 Size: 552 939 1077 3811 Fragments arranged by size: 3811 1077 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2433 3895 4689 4709 2433 Size: 1462 794 20 4103 Fragments arranged by size: 4103 1462 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2476 2511 3340 3375 529 Size: 35 1912 35 829 35 3533 Fragments arranged by size: 3533 1912 829 35 35 35 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DraIII CAC_nnn'GTG Cuts at: 3468 3468 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3826 4715 Size: 54 1425 50 275 18 177 1819 889 Cuts at: 4715 6344 8 Size: 1629 43 Fragments arranged by size: 1819 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 3114 4309 1353 Size: 717 1044 1195 3423 Fragments arranged by size: 3423 1195 1044 717 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2070 2070 Size: 6379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2549 2558 2642 2680 3516 4442 Size: 393 612 305 9 84 38 836 926 Cuts at: 4442 5039 5826 6094 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 926 836 787 612 597 393 305 268 84 38 9 EcoRI G'AATT_C Cuts at: 2370 2879 3688 4066 2370 Size: 509 809 378 4683 Fragments arranged by size: 4683 809 509 378 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2428 2450 2460 2482 5020 Size: 32 135 32 856 22 10 22 2538 Cuts at: 5020 5052 5094 5126 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2538 856 135 42 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 2469 3723 3969 669 Size: 988 281 531 1254 246 3079 Fragments arranged by size: 3079 1254 988 531 281 246 HindIII A'AGCT_T Cuts at: 748 3581 3624 748 Size: 2833 43 3503 Fragments arranged by size: 3503 2833 43 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2284 3246 3624 5493 5677 1305 Size: 979 962 378 1869 184 2007 Fragments arranged by size: 2007 1869 979 962 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4380 Fragments arranged by size: 4380 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2062 3380 4337 4696 4855 518 Size: 648 896 1318 957 359 159 2042 Fragments arranged by size: 2042 1318 957 896 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3795 4472 5620 5865 1606 Size: 2189 677 1148 245 2120 Fragments arranged by size: 2189 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3248 3248 Size: 6379 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 NdeI CA'TA_TG Cuts at: 387 2059 2683 3379 387 Size: 1672 624 696 3387 Fragments arranged by size: 3387 1672 696 624 NgoMIV G'CCGG_C Cuts at: 3246 3246 Size: 6379 NheI G'CTAG_C Cuts at: 1052 3425 1052 Size: 2373 4006 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2278 3737 5282 1132 Size: 97 1049 1459 1545 2229 Fragments arranged by size: 2229 1545 1459 1049 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 2274 5278 2274 Size: 3004 3375 PflMI CCAn_nnn'nTGG Cuts at: 1108 2327 1108 Size: 1219 5160 PfoI T'CCnGG_A Cuts at: 2550 2550 Size: 6379 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PstI C_TGCA'G Cuts at: 830 3261 3541 3731 830 Size: 2431 280 190 3478 Fragments arranged by size: 3478 2431 280 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 SacI G_AGCT'C Cuts at: 2072 2072 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 2987 4627 6339 2987 Size: 1640 1712 3027 Fragments arranged by size: 3027 1712 1640 SexAI A'CCwGG_T Cuts at: 3528 3528 Size: 6379 SfcI C'TryA_G Cuts at: 826 1045 2753 3257 3537 3727 4862 5543 5734 Size: 219 1708 504 280 190 1135 681 191 Cuts at: 5734 826 Size: 1471 Fragments arranged by size: 1708 1471 1135 681 504 280 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3185 4483 5384 5646 820 Size: 197 926 780 462 1298 901 262 1553 Fragments arranged by size: 1553 1298 926 901 780 462 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2004 2909 3093 3818 513 Size: 552 939 905 184 725 3074 Fragments arranged by size: 3074 939 905 725 552 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3218 4406 4423 4576 4761 5206 325 Size: 1255 1638 1188 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1255 1188 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2985 3560 Size: 274 80 33 51 1443 300 708 575 Cuts at: 3560 3576 4625 6337 96 Size: 16 1049 1712 138 Fragments arranged by size: 1712 1443 1049 708 575 300 274 138 80 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 6339 6371 2762 Size: 32 3545 32 2770 Fragments arranged by size: 3545 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 3206 4508 3206 Size: 1302 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI