Restriction map for
pFN21AB9461
(Linear) (Six-base) MAPSORT of: KIBB9461.seq Check: 4174 from: 1 to: 1674
KIBB9461.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 970 1181 1674
Size: 970 211 493
Fragments arranged by size:
970 493 211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 637 1674
Size: 544 93 1037
Fragments arranged by size:
1037 544 93
AflII C'TTAA_G
Cuts at: 0 1180 1674
Size: 1180 494
AflIII A'CryG_T
Cuts at: 0 269 1674
Size: 269 1405
AleI CACnn'nnGTG
Cuts at: 0 1375 1674
Size: 1375 299
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1271 1303 1674
Size: 1271 32 371
Fragments arranged by size:
1271 371 32
AlwNI CAG_nnn'CTG
Cuts at: 0 633 1674
Size: 633 1041
ApaLI G'TGCA_C
Cuts at: 0 1464 1674
Size: 1464 210
ApoI r'AATT_y
Cuts at: 0 365 670 700 874 1234 1674
Size: 365 305 30 174 360 440
Fragments arranged by size:
440 365 360 305 174 30
AvaI C'yCGr_G
Cuts at: 0 919 1674
Size: 919 755
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1594 1627 1674
Size: 1594 33 47
Fragments arranged by size:
1594 47 33
BamHI G'GATC_C
Cuts at: 0 164 1674
Size: 164 1510
BanI G'GyrC_C
Cuts at: 0 694 1674
Size: 694 980
BanII G_rGCy'C
Cuts at: 0 67 1674
Size: 67 1607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 676 710 1674
Size: 676 34 964
Fragments arranged by size:
964 676 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 429 1674
Size: 429 1245
BclI T'GATC_A
Cuts at: 0 506 1674
Size: 506 1168
BglII A'GATC_T
Cuts at: 0 1001 1674
Size: 1001 673
Bme1580I G_kGCm'C
Cuts at: 0 1468 1674
Size: 1468 206
BmtI G_CTAG'C
Cuts at: 0 1424 1674
Size: 1424 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 553 675 1511 1674
Size: 239 314 122 836 163
Fragments arranged by size:
836 314 239 163 122
Bpu10I CC'TnA_GC
Cuts at: 0 496 1674
Size: 496 1178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1674
Size: 703 971
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 729 1674
Size: 349 380 945
Fragments arranged by size:
945 380 349
BsaWI w'CCGG_w
Cuts at: 0 1481 1674
Size: 1481 193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 91 1674
Size: 88 3 1583
Fragments arranged by size:
1583 88 3
BseYI C'CCAG_C
Cuts at: 0 1341 1506 1674
Size: 1341 165 168
Fragments arranged by size:
1341 168 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1607 1674
Size: 1607 67
BsiHKAI G_wGCw'C
Cuts at: 0 67 1468 1674
Size: 67 1401 206
Fragments arranged by size:
1401 206 67
Bsp1286I G_dGCh'C
Cuts at: 0 67 1468 1674
Size: 67 1401 206
Fragments arranged by size:
1401 206 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 970 1181 1259 1674
Size: 970 211 78 415
Fragments arranged by size:
970 415 211 78
BsrDI GCAATG_nn'
Cuts at: 0 605 1674
Size: 605 1069
BsrFI r'CCGG_y
Cuts at: 0 1241 1674
Size: 1241 433
BsrGI T'GTAC_A
Cuts at: 0 272 1571 1674
Size: 272 1299 103
Fragments arranged by size:
1299 272 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1674
Size: 705 969
BstYI r'GATC_y
Cuts at: 0 164 343 1001 1674
Size: 164 179 658 673
Fragments arranged by size:
673 658 179 164
BtgI C'CryG_G
Cuts at: 0 1076 1674
Size: 1076 598
BtsI GCAGTG_nn'
Cuts at: 0 428 1674
Size: 428 1246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 471 506 1335 1370 1674
Size: 471 35 829 35 304
Fragments arranged by size:
829 471 304 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1463 1674
Size: 1463 211
EaeI y'GGCC_r
Cuts at: 0 2 1674
Size: 2 1672
EarI CTCTTCn'nnn_
Cuts at: 0 65 1109 1674
Size: 65 1044 565
Fragments arranged by size:
1044 565 65
EcoICRI GAG'CTC
Cuts at: 0 65 1674
Size: 65 1609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 544 553 637 675 1511 1674
Size: 239 305 9 84 38 836 163
Fragments arranged by size:
836 305 239 163 84 38 9
EcoRI G'AATT_C
Cuts at: 0 365 874 1674
Size: 365 509 800
Fragments arranged by size:
800 509 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 423 445 455 477 1674
Size: 423 22 10 22 1197
Fragments arranged by size:
1197 423 22 22 10
HincII GTy'rAC
Cuts at: 0 464 1674
Size: 464 1210
HindIII A'AGCT_T
Cuts at: 0 1576 1619 1674
Size: 1576 43 55
Fragments arranged by size:
1576 55 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1241 1619 1674
Size: 279 962 378 55
Fragments arranged by size:
962 378 279 55
MscI TGG'CCA
Cuts at: 0 4 1674
Size: 4 1670
MslI CAynn'nnrTG
Cuts at: 0 57 1375 1674
Size: 57 1318 299
Fragments arranged by size:
1318 299 57
NaeI GCC'GGC
Cuts at: 0 1243 1674
Size: 1243 431
NdeI CA'TA_TG
Cuts at: 0 54 678 1374 1674
Size: 54 624 696 300
Fragments arranged by size:
696 624 300 54
NgoMIV G'CCGG_C
Cuts at: 0 1241 1674
Size: 1241 433
NheI G'CTAG_C
Cuts at: 0 1420 1674
Size: 1420 254
NspI r_CATG'y
Cuts at: 0 273 1674
Size: 273 1401
PciI A'CATG_T
Cuts at: 0 269 1674
Size: 269 1405
PflMI CCAn_nnn'nTGG
Cuts at: 0 322 1674
Size: 322 1352
PfoI T'CCnGG_A
Cuts at: 0 545 1674
Size: 545 1129
PstI C_TGCA'G
Cuts at: 0 1256 1536 1674
Size: 1256 280 138
Fragments arranged by size:
1256 280 138
SacI G_AGCT'C
Cuts at: 0 67 1674
Size: 67 1607
ScaI AGT'ACT
Cuts at: 0 982 1674
Size: 982 692
SexAI A'CCwGG_T
Cuts at: 0 1523 1674
Size: 1523 151
SfcI C'TryA_G
Cuts at: 0 748 1252 1532 1674
Size: 748 504 280 142
Fragments arranged by size:
748 504 280 142
SmlI C'TyrA_G
Cuts at: 0 718 1180 1674
Size: 718 462 494
Fragments arranged by size:
718 494 462
StyI C'CwwG_G
Cuts at: 0 904 1088 1674
Size: 904 184 586
Fragments arranged by size:
904 586 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1213 1674
Size: 1213 461
TatI w'GTAC_w
Cuts at: 0 272 980 1555 1571 1674
Size: 272 708 575 16 103
Fragments arranged by size:
708 575 272 103 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 1674
Size: 757 32 885
Fragments arranged by size:
885 757 32
XmnI GAAnn'nnTTC
Cuts at: 0 1201 1674
Size: 1201 473
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AloI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BcgI BciVI
BclI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstXI BstYI BtgI BtsI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoRI Hin4I HincII HindIII
MmeI MscI MslI NaeI NdeI NgoMIV NheI NspI
PciI PflMI PfoI PstI SacI ScaI SexAI SfcI
SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
ApaI AscI AseI AsiSI AvrII BbeI BbsI BbvCI
BfrBI BglI BlpI BmgBI BmrI BplI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DrdI EagI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MspA1I NarI NcoI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI