(Circular) (Six-base) MAPSORT of: pFN21AB9459.seq Check: 8573 from: 1 to: 8377 pFN21AB9459 8377 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4405 4405 Size: 8377 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6187 278 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3738 5720 1937 Size: 1801 1982 4594 Fragments arranged by size: 4594 1982 1801 Acc65I G'GTAC_C Cuts at: 5698 5698 Size: 8377 AclI AA'CG_TT Cuts at: 1563 6504 6877 1563 Size: 4941 373 3063 Fragments arranged by size: 4941 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3249 3344 3930 4271 4731 5000 5027 6440 Size: 1617 95 586 341 460 269 27 1413 Cuts at: 6440 7824 8092 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1617 1413 1384 586 460 341 269 268 95 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8180 AflIII A'CryG_T Cuts at: 1203 3501 3508 7276 8253 1203 Size: 2298 7 3768 977 1327 Fragments arranged by size: 3768 2298 1327 977 7 AgeI A'CCGG_T Cuts at: 7208 7208 Size: 8377 AhdI GACnn_n'nnGTC Cuts at: 7106 7106 Size: 8377 AleI CACnn'nnGTG Cuts at: 1166 2619 3719 3964 5618 1166 Size: 1453 1100 245 1654 3925 Fragments arranged by size: 3925 1654 1453 1100 245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3413 1184 Size: 2229 6148 AlwNI CAG_nnn'CTG Cuts at: 2197 4921 6120 7692 2197 Size: 2724 1199 1572 2882 Fragments arranged by size: 2882 2724 1572 1199 ApaI G_GGCC'C Cuts at: 5403 5403 Size: 8377 ApaLI G'TGCA_C Cuts at: 2125 5300 6434 7590 2125 Size: 3175 1134 1156 2912 Fragments arranged by size: 3175 2912 1156 1134 ApoI r'AATT_y Cuts at: 1493 2015 3797 5066 5087 5686 5912 6064 8173 Size: 522 1782 1269 21 599 226 152 2109 Cuts at: 8173 1493 Size: 1697 Fragments arranged by size: 2109 1782 1697 1269 599 522 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 6931 160 Size: 872 5899 1606 Fragments arranged by size: 5899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 AvaI C'yCGr_G Cuts at: 1870 1943 3765 4896 5396 5702 1870 Size: 73 1822 1131 500 306 4545 Fragments arranged by size: 4545 1822 1131 500 306 73 BamHI G'GATC_C Cuts at: 2073 4245 5707 6074 2073 Size: 2172 1462 367 4376 Fragments arranged by size: 4376 2172 1462 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 4463 5382 5698 7153 Size: 325 217 627 1565 1111 919 316 1455 Cuts at: 7153 618 Size: 1842 Fragments arranged by size: 1842 1565 1455 1111 919 627 325 316 217 BanII G_rGCy'C Cuts at: 1391 2049 4668 5403 5696 1391 Size: 658 2619 735 293 4072 Fragments arranged by size: 4072 2619 735 658 293 BbeI G_GCGC'C Cuts at: 5386 5386 Size: 8377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2451 2764 928 Size: 971 552 313 6541 Fragments arranged by size: 6541 971 552 313 BbvCI CC'TCA_GC Cuts at: 2798 2798 Size: 8377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6568 6602 1903 Size: 34 4631 34 3678 Fragments arranged by size: 4631 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4411 4453 6269 7479 4411 Size: 42 1816 1210 5309 Fragments arranged by size: 5309 1816 1210 42 BclI T'GATC_A Cuts at: 1548 2344 3312 3813 4789 1548 Size: 796 968 501 976 5136 Fragments arranged by size: 5136 976 968 796 501 BfrBI ATG'CAT Cuts at: 3301 3301 Size: 8377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6988 136 Size: 107 122 71 631 605 5316 1525 Fragments arranged by size: 5316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2694 3369 4657 4758 8372 2694 Size: 675 1288 101 3614 2699 Fragments arranged by size: 3614 2699 1288 675 101 BlpI GC'TnA_GC Cuts at: 2048 5794 2048 Size: 3746 4631 Bme1580I G_kGCm'C Cuts at: 1790 2129 2280 2406 3357 4731 5304 5403 6438 Size: 339 151 126 951 1374 573 99 1035 Cuts at: 6438 7594 1790 Size: 1156 2573 Fragments arranged by size: 2573 1374 1156 1035 951 573 339 151 126 99 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2568 2641 2859 Size: 416 53 83 399 24 1151 73 218 Cuts at: 2859 3678 6108 7066 442 Size: 819 2430 958 1753 Fragments arranged by size: 2430 1753 1151 958 819 416 399 218 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 3156 1056 Size: 2100 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 3875 3983 4373 4682 7037 1239 Size: 990 1646 108 390 309 2355 2579 Fragments arranged by size: 2579 2355 1646 990 390 309 108 Bpu10I CC'TnA_GC Cuts at: 2798 4674 2798 Size: 1876 6501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5333 6502 7367 7665 5333 Size: 1169 865 298 6045 Fragments arranged by size: 6045 1169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2865 7040 882 Size: 617 516 850 4175 2219 Fragments arranged by size: 4175 2219 850 617 516 BsaAI yAC'GTr Cuts at: 493 1204 6168 493 Size: 711 4964 2702 Fragments arranged by size: 4964 2702 711 BsaBI GATnn'nnATC Cuts at: 8371 8371 Size: 8377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5383 6184 6566 7201 Size: 53 83 186 936 3850 801 382 635 Cuts at: 7201 275 Size: 1451 Fragments arranged by size: 3850 1451 936 801 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6810 7208 7482 7629 8040 6810 Size: 398 274 147 411 7147 Fragments arranged by size: 7147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8152 Fragments arranged by size: 8152 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2226 4503 5087 1186 Size: 1040 2277 584 4476 Fragments arranged by size: 4476 2277 1040 584 BseYI C'CCAG_C Cuts at: 4023 4033 4536 4641 5154 7580 8076 4023 Size: 10 503 105 513 2426 496 4324 Fragments arranged by size: 4324 2426 513 503 496 105 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3824 4383 1163 Size: 573 2088 559 5157 Fragments arranged by size: 5157 2088 573 559 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2620 5827 6588 7616 1540 Size: 275 186 619 3207 761 1028 2301 Fragments arranged by size: 3207 2301 1028 761 619 275 186 BsiHKAI G_wGCw'C Cuts at: 2129 2428 4495 4668 5304 6438 6523 7594 2129 Size: 299 2067 173 636 1134 85 1071 2912 Fragments arranged by size: 2912 2067 1134 1071 636 299 173 85 BsmI GAATG_Cn' Cuts at: 3301 3859 4213 5888 5981 3301 Size: 558 354 1675 93 5697 Fragments arranged by size: 5697 1675 558 354 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2209 2209 Size: 8377 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2129 2280 2406 2428 3357 4495 Size: 399 259 80 151 126 22 929 1138 Cuts at: 4495 4668 4731 5304 5403 5696 6438 6523 7594 Size: 173 63 573 99 293 742 85 1071 Cuts at: 7594 1391 Size: 2174 Fragments arranged by size: 2174 1138 1071 929 742 573 399 293 259 173 151 126 99 85 80 63 22 BspEI T'CCGG_A Cuts at: 8040 8040 Size: 8377 BspHI T'CATG_A Cuts at: 2311 3577 4834 6266 7996 2311 Size: 1266 1257 1432 1730 2692 Fragments arranged by size: 2692 1730 1432 1266 1257 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2744 4405 5298 5732 844 Size: 1900 1661 893 434 3489 Fragments arranged by size: 3489 1900 1661 893 434 BsrBI CCG'CTC Cuts at: 1394 1823 6264 1394 Size: 429 4441 3507 Fragments arranged by size: 4441 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2267 4467 6872 7046 65 Size: 1183 1019 2200 2405 174 1396 Fragments arranged by size: 2405 2200 1396 1183 1019 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7021 7208 1700 Size: 110 5211 187 2869 Fragments arranged by size: 5211 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3143 3183 96 Size: 3047 40 5290 Fragments arranged by size: 5290 3047 40 BssHII G'CGCG_C Cuts at: 1925 2031 1925 Size: 106 8271 BssSI C'ACGA_G Cuts at: 6437 7449 6437 Size: 1012 7365 BstAPI GCAn_nnn'nTGC Cuts at: 794 3128 4499 5299 794 Size: 2334 1371 800 3872 Fragments arranged by size: 3872 2334 1371 800 BstBI TT'CG_AA Cuts at: 3688 4812 7181 3688 Size: 1124 2369 4884 Fragments arranged by size: 4884 2369 1124 BstEII G'GTnAC_C Cuts at: 1184 5611 1184 Size: 4427 3950 BstXI CCAn_nnnn'nTGG Cuts at: 2939 4006 2939 Size: 1067 7310 BstYI r'GATC_y Cuts at: 1972 2073 2694 3369 4245 4657 4758 5707 6074 Size: 101 621 675 876 412 101 949 367 Cuts at: 6074 6459 6476 7928 8014 8026 8372 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 949 876 675 621 412 385 367 346 101 101 86 17 12 BstZ17I GTA'TAC Cuts at: 3739 3739 Size: 8377 Bsu36I CC'TnA_GG Cuts at: 3244 5409 3244 Size: 2165 6212 BtgI C'CryG_G Cuts at: 513 1065 3676 4297 5199 5536 513 Size: 552 2611 621 902 337 3354 Fragments arranged by size: 3354 2611 902 621 552 337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7638 Fragments arranged by size: 7638 683 56 BtsI GCAGTG_nn' Cuts at: 2595 3917 5893 6687 6707 2595 Size: 1322 1976 794 20 4265 Fragments arranged by size: 4265 1976 1322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8342 DraI TTT'AAA Cuts at: 5681 5681 Size: 8377 DraIII CAC_nnn'GTG Cuts at: 3847 5567 3847 Size: 1720 6657 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7384 809 Size: 507 6068 1802 Fragments arranged by size: 6068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2972 3868 4195 Size: 54 1425 50 275 18 1142 896 327 Cuts at: 4195 5824 6713 8342 8 Size: 1629 889 1629 43 Fragments arranged by size: 1629 1629 1425 1142 896 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5824 1537 Size: 275 4012 4090 Fragments arranged by size: 4090 4012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6307 1353 Size: 4954 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6964 7338 7484 387 Size: 1064 138 5375 374 146 1280 Fragments arranged by size: 5375 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4666 4666 Size: 8377 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 3249 3344 3875 3930 3983 4271 Size: 393 597 1020 95 531 55 53 288 Cuts at: 4271 4373 4682 4731 5000 5027 6440 7037 7824 Size: 102 309 49 269 27 1413 597 787 Cuts at: 7824 8092 1239 Size: 268 1524 Fragments arranged by size: 1524 1413 1020 787 597 597 531 393 309 288 269 268 102 95 55 53 49 27 EcoNI CCTnn'n_nnAGG Cuts at: 4052 4052 Size: 8377 EcoO109I rG'GnC_Cy Cuts at: 2890 3603 5571 2890 Size: 713 1968 5696 Fragments arranged by size: 5696 1968 713 EcoRI G'AATT_C Cuts at: 5686 6064 5686 Size: 378 7999 FspI TGC'GCA Cuts at: 6883 6883 Size: 8377 HaeII r_GCGC'y Cuts at: 1678 2257 5386 7524 1678 Size: 579 3129 2138 2531 Fragments arranged by size: 3129 2531 2138 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2113 2145 2410 Size: 32 135 32 486 32 23 32 265 Cuts at: 2410 2442 3191 3223 3304 3336 3398 3430 5647 Size: 32 749 32 81 32 62 32 2217 Cuts at: 5647 5679 7018 7050 7092 7124 1373 Size: 32 1339 32 42 32 2626 Fragments arranged by size: 2626 2217 1339 749 486 265 135 81 62 42 32 32 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2397 5721 5967 669 Size: 988 281 72 387 3324 246 3079 Fragments arranged by size: 3324 3079 988 387 281 246 72 HindIII A'AGCT_T Cuts at: 748 2246 5009 748 Size: 1498 2763 4116 Fragments arranged by size: 4116 2763 1498 HpaI GTT'AAC Cuts at: 2010 2397 5967 2010 Size: 387 3570 4420 Fragments arranged by size: 4420 3570 387 KasI G'GCGC_C Cuts at: 5382 5382 Size: 8377 KpnI G_GTAC'C Cuts at: 5702 5702 Size: 8377 MfeI C'AATT_G Cuts at: 4423 5976 4423 Size: 1553 6824 MluI A'CGCG_T Cuts at: 8253 8253 Size: 8377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4542 4775 5050 7491 7675 1305 Size: 3237 233 275 2441 184 2007 Fragments arranged by size: 3237 2441 2007 275 233 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3870 4197 10 Size: 54 1425 343 2038 327 4190 Fragments arranged by size: 4190 2038 1425 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2619 2937 3719 3964 5618 6335 Size: 648 1191 262 318 782 245 1654 717 Cuts at: 6335 6694 6853 518 Size: 359 159 2042 Fragments arranged by size: 2042 1654 1191 782 717 648 359 318 262 245 159 MspA1I CmG'CkG Cuts at: 1606 3034 4289 4700 4997 5038 5366 5793 6470 Size: 1428 1255 411 297 41 328 427 677 Cuts at: 6470 7618 7863 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1428 1255 1148 677 427 411 328 297 245 41 NarI GG'CG_CC Cuts at: 5383 5383 Size: 8377 NcoI C'CATG_G Cuts at: 513 1065 4297 513 Size: 552 3232 4593 Fragments arranged by size: 4593 3232 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8377 NheI G'CTAG_C Cuts at: 1052 3152 1052 Size: 2100 6277 NotI GC'GGCC_GC Cuts at: 5824 5824 Size: 8377 NsiI A_TGCA'T Cuts at: 3303 3303 Size: 8377 NspI r_CATG'y Cuts at: 1132 1229 2603 3505 3512 5491 5735 7280 1132 Size: 97 1374 902 7 1979 244 1545 2229 Fragments arranged by size: 2229 1979 1545 1374 902 244 97 7 PasI CC'CwG_GG Cuts at: 1348 4691 1348 Size: 3343 5034 PciI A'CATG_T Cuts at: 3501 3508 7276 3501 Size: 7 3768 4602 Fragments arranged by size: 4602 3768 7 PflMI CCAn_nnn'nTGG Cuts at: 1108 4072 5567 1108 Size: 2964 1495 3918 Fragments arranged by size: 3918 2964 1495 PmeI GTTT'AAAC Cuts at: 5681 5681 Size: 8377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6446 6478 7959 7991 6446 Size: 32 1481 32 6832 Fragments arranged by size: 6832 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2890 2890 Size: 8377 PshAI GACnn'nnGTC Cuts at: 1613 4267 1613 Size: 2654 5723 PsiI TTA'TAA Cuts at: 5947 5947 Size: 8377 PspOMI G'GGCC_C Cuts at: 5399 5399 Size: 8377 PstI C_TGCA'G Cuts at: 830 4056 4560 5295 5609 5670 5729 830 Size: 3226 504 735 314 61 59 3478 Fragments arranged by size: 3478 3226 735 504 314 61 59 PvuI CG_AT'CG Cuts at: 2001 2620 2001 Size: 619 7758 PvuII CAG'CTG Cuts at: 3034 4289 4700 4997 5038 5366 3034 Size: 1255 411 297 41 328 6045 Fragments arranged by size: 6045 1255 411 328 297 41 SacI G_AGCT'C Cuts at: 4668 4668 Size: 8377 SalI G'TCGA_C Cuts at: 1936 5719 1936 Size: 3783 4594 SbfI CC_TGCA'GG Cuts at: 5729 5729 Size: 8377 ScaI AGT'ACT Cuts at: 6625 8337 6625 Size: 1712 6665 SfcI C'TryA_G Cuts at: 826 1045 2580 4052 4556 4587 4883 5291 5605 Size: 219 1535 1472 504 31 296 408 314 Cuts at: 5605 5666 5725 6860 7541 7732 826 Size: 61 59 1135 681 191 1471 Fragments arranged by size: 1535 1472 1471 1135 681 504 408 314 296 219 191 61 59 31 SfoI GGC'GCC Cuts at: 5384 5384 Size: 8377 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8377 SmaI CCC'GGG Cuts at: 1872 3767 5704 1872 Size: 1895 1937 4545 Fragments arranged by size: 4545 1937 1895 SmlI C'TyrA_G Cuts at: 820 1017 1943 5348 6481 7382 7644 820 Size: 197 926 3405 1133 901 262 1553 Fragments arranged by size: 3405 1553 1133 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6168 493 Size: 5675 2702 SpeI A'CTAG_T Cuts at: 152 2081 3862 152 Size: 1929 1781 4667 Fragments arranged by size: 4667 1929 1781 SphI G_CATG'C Cuts at: 1132 5735 1132 Size: 4603 3774 SspI AAT'ATT Cuts at: 5 52 6301 5 Size: 47 6249 2081 Fragments arranged by size: 6249 2081 47 StuI AGG'CCT Cuts at: 1517 3249 1517 Size: 1732 6645 StyI C'CwwG_G Cuts at: 513 1065 2069 4297 4737 5816 513 Size: 552 1004 2228 440 1079 3074 Fragments arranged by size: 3074 2228 1079 1004 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3033 4211 5294 6404 6421 6574 6759 Size: 1255 1453 1178 1083 1110 17 153 185 Cuts at: 6759 7204 325 Size: 445 1498 Fragments arranged by size: 1498 1453 1255 1178 1110 1083 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2602 3143 3183 Size: 274 80 33 51 1443 625 541 40 Cuts at: 3183 5181 6623 8335 96 Size: 1998 1442 1712 138 Fragments arranged by size: 1998 1712 1443 1442 625 541 274 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2317 2349 3766 3798 8337 8369 2317 Size: 32 1417 32 4539 32 2325 Fragments arranged by size: 4539 2325 1417 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8377 XbaI T'CTAG_A Cuts at: 2375 3160 5236 5713 2375 Size: 785 2076 477 5039 Fragments arranged by size: 5039 2076 785 477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 4758 5410 2446 Size: 2312 652 5413 Fragments arranged by size: 5413 2312 652 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8377 XmaI C'CCGG_G Cuts at: 1870 3765 5702 1870 Size: 1895 1937 4545 Fragments arranged by size: 4545 1937 1895 XmnI GAAnn'nnTTC Cuts at: 4149 6506 4149 Size: 2357 6020 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6185 276 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BplI BsiWI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI