(Circular) (Six-base) MAPSORT of: pFN21AB9458.seq Check: 1417 from: 1 to: 7216 pFN21AB9458 7216 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3176 3908 4171 3176 Size: 732 263 6221 Fragments arranged by size: 6221 732 263 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2682 5026 278 Size: 53 83 186 936 1146 2344 2468 Fragments arranged by size: 2468 2344 1146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3321 3456 3747 4559 1937 Size: 1384 135 291 812 4594 Fragments arranged by size: 4594 1384 812 291 135 Acc65I G'GTAC_C Cuts at: 4537 4537 Size: 7216 AclI AA'CG_TT Cuts at: 1563 5343 5716 1563 Size: 3780 373 3063 Fragments arranged by size: 3780 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 2990 3574 4210 4484 5279 6663 6931 Size: 947 411 584 636 274 795 1384 268 Cuts at: 6931 1632 Size: 1917 Fragments arranged by size: 1917 1384 947 795 636 584 411 274 268 AfeI AGC'GCT Cuts at: 2391 2391 Size: 7216 AflII C'TTAA_G Cuts at: 820 1017 4139 820 Size: 197 3122 3897 Fragments arranged by size: 3897 3122 197 AflIII A'CryG_T Cuts at: 1203 6115 7092 1203 Size: 4912 977 1327 Fragments arranged by size: 4912 1327 977 AgeI A'CCGG_T Cuts at: 2203 4215 6047 2203 Size: 2012 1832 3372 Fragments arranged by size: 3372 2012 1832 AhdI GACnn_n'nnGTC Cuts at: 5945 5945 Size: 7216 AleI CACnn'nnGTG Cuts at: 1166 3578 3779 4265 1166 Size: 2412 201 486 4117 Fragments arranged by size: 4117 2412 486 201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2876 4193 1184 Size: 1692 1317 4207 Fragments arranged by size: 4207 1692 1317 AlwNI CAG_nnn'CTG Cuts at: 2316 2453 3559 4359 4959 6531 2316 Size: 137 1106 800 600 1572 3001 Fragments arranged by size: 3001 1572 1106 800 600 137 ApaI G_GGCC'C Cuts at: 2410 3129 2410 Size: 719 6497 ApaLI G'TGCA_C Cuts at: 2770 3131 5273 6429 2770 Size: 361 2142 1156 3557 Fragments arranged by size: 3557 2142 1156 361 ApoI r'AATT_y Cuts at: 1493 4525 4751 4903 7012 1493 Size: 3032 226 152 2109 1697 Fragments arranged by size: 3032 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5770 160 Size: 872 4738 1606 Fragments arranged by size: 4738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 AvaI C'yCGr_G Cuts at: 1870 1943 2398 3080 4288 4404 4427 4541 1870 Size: 73 455 682 1208 116 23 114 4545 Fragments arranged by size: 4545 1208 682 455 116 114 73 23 BamHI G'GATC_C Cuts at: 4546 4913 4546 Size: 367 6849 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 3090 4537 5992 618 Size: 325 217 627 419 884 1447 1455 1842 Fragments arranged by size: 1842 1455 1447 884 627 419 325 217 BanII G_rGCy'C Cuts at: 1391 2399 2410 2985 3081 3129 4535 1391 Size: 1008 11 575 96 48 1406 4072 Fragments arranged by size: 4072 1406 1008 575 96 48 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2257 2471 3515 3748 3804 3967 4290 Size: 971 358 214 1044 233 56 163 323 Cuts at: 4290 4477 928 Size: 187 3667 Fragments arranged by size: 3667 1044 971 358 323 233 214 187 163 56 BbvCI CC'TCA_GC Cuts at: 4479 4479 Size: 7216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5407 5441 1903 Size: 34 3470 34 3678 Fragments arranged by size: 3678 3470 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5108 6318 5108 Size: 1210 6006 BclI T'GATC_A Cuts at: 1548 3336 1548 Size: 1788 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5827 136 Size: 107 122 71 631 605 4155 1525 Fragments arranged by size: 4155 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7211 7211 Size: 7216 BlpI GC'TnA_GC Cuts at: 4422 4633 4422 Size: 211 7005 Bme1580I G_kGCm'C Cuts at: 1790 2410 2774 3129 3135 5277 6433 1790 Size: 620 364 355 6 2142 1156 2573 Fragments arranged by size: 2573 2142 1156 620 364 355 6 BmgBI CAC'GTC Cuts at: 1318 3834 1318 Size: 2516 4700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4947 5905 442 Size: 416 53 83 399 24 3530 958 1753 Fragments arranged by size: 3530 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7216 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2157 3196 2157 Size: 1039 6177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2004 2015 2338 2552 2996 3044 3209 3426 Size: 765 11 323 214 444 48 165 217 Cuts at: 3426 3447 3926 3962 5876 1239 Size: 21 479 36 1914 2579 Fragments arranged by size: 2579 1914 765 479 444 323 217 214 165 48 36 21 11 Bpu10I CC'TnA_GC Cuts at: 4165 4319 4479 4165 Size: 154 160 6902 Fragments arranged by size: 6902 160 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3584 5341 6206 6504 3584 Size: 1757 865 298 4296 Fragments arranged by size: 4296 1757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 3209 3717 4464 5879 882 Size: 617 504 1206 508 747 1415 2219 Fragments arranged by size: 2219 1415 1206 747 617 508 504 BsaAI yAC'GTr Cuts at: 493 1204 5007 493 Size: 711 3803 2702 Fragments arranged by size: 3803 2702 711 BsaBI GATnn'nnATC Cuts at: 2939 3686 7210 2939 Size: 747 3524 2945 Fragments arranged by size: 3524 2945 747 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2679 3005 4305 5023 Size: 53 83 186 936 1146 326 1300 718 Cuts at: 5023 5405 6040 275 Size: 382 635 1451 Fragments arranged by size: 1451 1300 1146 936 718 635 382 326 186 83 53 BsaWI w'CCGG_w Cuts at: 2203 4215 5649 6047 6321 6468 6879 2203 Size: 2012 1434 398 274 147 411 2540 Fragments arranged by size: 2540 2012 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 2664 2694 3068 Size: 30 165 30 599 30 632 30 374 Cuts at: 3068 3098 1178 Size: 30 5296 Fragments arranged by size: 5296 632 599 374 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 2993 3127 3465 1186 Size: 924 883 134 338 4937 Fragments arranged by size: 4937 924 883 338 134 BseYI C'CCAG_C Cuts at: 2042 2386 2392 2410 2782 3023 6419 6915 2042 Size: 344 6 18 372 241 3396 496 2343 Fragments arranged by size: 3396 2343 496 372 344 241 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 2600 2849 2942 3106 1163 Size: 573 744 120 249 93 164 5273 Fragments arranged by size: 5273 744 573 249 164 120 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2826 2898 3500 4666 5427 6455 Size: 275 186 825 72 602 1166 761 1028 Cuts at: 6455 1540 Size: 2301 Fragments arranged by size: 2301 1166 1028 825 761 602 275 186 72 BsiHKAI G_wGCw'C Cuts at: 2774 2985 3135 4405 5277 5362 6433 2774 Size: 211 150 1270 872 85 1071 3557 Fragments arranged by size: 3557 1270 1071 872 211 150 85 BsmI GAATG_Cn' Cuts at: 4727 4820 4727 Size: 93 7123 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2626 2186 Size: 440 6776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2399 2410 2774 2985 3081 3129 3135 Size: 399 609 11 364 211 96 48 6 Cuts at: 3135 4405 4535 5277 5362 6433 1391 Size: 1270 130 742 85 1071 2174 Fragments arranged by size: 2174 1270 1071 742 609 399 364 211 130 96 85 48 11 6 BspEI T'CCGG_A Cuts at: 6879 6879 Size: 7216 BspHI T'CATG_A Cuts at: 3699 5105 6835 3699 Size: 1406 1730 4080 Fragments arranged by size: 4080 1730 1406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2502 3176 3908 4171 4571 844 Size: 1658 674 732 263 400 3489 Fragments arranged by size: 3489 1658 732 674 400 263 BsrBI CCG'CTC Cuts at: 1394 1823 4164 5103 1394 Size: 429 2341 939 3507 Fragments arranged by size: 3507 2341 939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4035 5711 5885 65 Size: 1183 2787 1676 174 1396 Fragments arranged by size: 2787 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2203 4215 5860 6047 1700 Size: 110 393 2012 1645 187 2869 Fragments arranged by size: 2869 2012 1645 393 187 110 BsrGI T'GTAC_A Cuts at: 96 2925 3330 96 Size: 2829 405 3982 Fragments arranged by size: 3982 2829 405 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7216 BssSI C'ACGA_G Cuts at: 2122 5276 6288 2122 Size: 3154 1012 3050 Fragments arranged by size: 3154 3050 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7216 BstBI TT'CG_AA Cuts at: 6020 6020 Size: 7216 BstEII G'GTnAC_C Cuts at: 1184 2650 1184 Size: 1466 5750 BstXI CCAn_nnnn'nTGG Cuts at: 2782 3781 3904 3931 2782 Size: 999 123 27 6067 Fragments arranged by size: 6067 999 123 27 BstYI r'GATC_y Cuts at: 1972 2313 3069 3432 4546 4913 5298 5315 6767 Size: 341 756 363 1114 367 385 17 1452 Cuts at: 6767 6853 6865 7211 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1114 756 385 367 363 346 341 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2257 2257 Size: 7216 BtgI C'CryG_G Cuts at: 513 1065 2004 2051 2695 3348 3369 3576 3618 Size: 552 939 47 644 653 21 207 42 Cuts at: 3618 3667 513 Size: 49 4062 Fragments arranged by size: 4062 939 653 644 552 207 49 47 42 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2605 487 Size: 683 56 1379 5098 Fragments arranged by size: 5098 1379 683 56 BtsI GCAGTG_nn' Cuts at: 2612 4732 5526 5546 2612 Size: 2120 794 20 4282 Fragments arranged by size: 4282 2120 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7216 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7181 DraI TTT'AAA Cuts at: 4520 4520 Size: 7216 DraIII CAC_nnn'GTG Cuts at: 3114 3114 Size: 7216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3287 4481 6223 809 Size: 507 1971 1194 1742 1802 Fragments arranged by size: 1971 1802 1742 1194 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2763 2823 Size: 54 1425 50 275 18 224 709 60 Cuts at: 2823 2883 2895 3497 3570 4663 5552 7181 8 Size: 60 12 602 73 1093 889 1629 43 Fragments arranged by size: 1629 1425 1093 889 709 602 275 224 73 60 60 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2823 2895 3497 4663 1537 Size: 275 1011 72 602 1166 4090 Fragments arranged by size: 4090 1166 1011 602 275 72 EarI CTCTTCn'nnn_ Cuts at: 1353 2338 4124 5146 1353 Size: 985 1786 1022 3423 Fragments arranged by size: 3423 1786 1022 985 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5803 6177 6323 387 Size: 1064 138 4214 374 146 1280 Fragments arranged by size: 4214 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2983 2983 Size: 7216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2015 2338 2552 2579 2990 2996 Size: 393 372 11 323 214 27 411 6 Cuts at: 2996 3044 3209 3426 3447 3574 3926 3962 4210 Size: 48 165 217 21 127 352 36 248 Cuts at: 4210 4484 5279 5876 6663 6931 1239 Size: 274 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 411 393 372 352 323 274 268 248 217 214 165 127 48 36 27 21 11 6 EcoNI CCTnn'n_nnAGG Cuts at: 2457 2876 3361 2457 Size: 419 485 6312 Fragments arranged by size: 6312 485 419 EcoO109I rG'GnC_Cy Cuts at: 2406 2407 3851 3864 2406 Size: 1 1444 13 5758 Fragments arranged by size: 5758 1444 13 1 EcoRI G'AATT_C Cuts at: 4525 4903 4525 Size: 378 6838 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3316 3991 3316 Size: 675 6541 FspI TGC'GCA Cuts at: 5722 5722 Size: 7216 HaeII r_GCGC'y Cuts at: 1678 2393 2838 6363 1678 Size: 715 445 3525 2531 Fragments arranged by size: 3525 2531 715 445 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2664 2696 3208 Size: 32 135 32 430 32 630 32 512 Cuts at: 3208 3240 3422 3454 3818 3850 4400 4432 5857 Size: 32 182 32 364 32 550 32 1425 Cuts at: 5857 5889 5931 5963 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 630 550 512 430 364 182 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2572 4060 4560 4806 669 Size: 988 281 634 1488 500 246 3079 Fragments arranged by size: 3079 1488 988 634 500 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7216 HpaI GTT'AAC Cuts at: 4806 4806 Size: 7216 KpnI G_GTAC'C Cuts at: 4541 4541 Size: 7216 MfeI C'AATT_G Cuts at: 4815 4815 Size: 7216 MluI A'CGCG_T Cuts at: 7092 7092 Size: 7216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6330 6514 1305 Size: 5025 184 2007 Fragments arranged by size: 5025 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3572 10 Size: 54 1425 343 1740 3654 Fragments arranged by size: 3654 1740 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3578 3779 3929 4265 5174 5533 5692 Size: 648 2412 201 150 336 909 359 159 Cuts at: 5692 518 Size: 2042 Fragments arranged by size: 2412 2042 909 648 359 336 201 159 150 MspA1I CmG'CkG Cuts at: 1606 2782 3010 3023 3064 3253 3669 3779 4632 Size: 1176 228 13 41 189 416 110 853 Cuts at: 4632 5309 6457 6702 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1176 1148 853 677 416 245 228 189 110 41 13 NcoI C'CATG_G Cuts at: 513 1065 2004 2695 3348 3618 513 Size: 552 939 691 653 270 4111 Fragments arranged by size: 4111 939 691 653 552 270 NdeI CA'TA_TG Cuts at: 387 387 Size: 7216 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7216 NotI GC'GGCC_GC Cuts at: 4663 4663 Size: 7216 NspI r_CATG'y Cuts at: 1132 1229 2926 3238 3655 4033 4574 6119 1132 Size: 97 1697 312 417 378 541 1545 2229 Fragments arranged by size: 2229 1697 1545 541 417 378 312 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7216 PciI A'CATG_T Cuts at: 6115 6115 Size: 7216 PflMI CCAn_nnn'nTGG Cuts at: 1108 2316 3682 1108 Size: 1208 1366 4642 Fragments arranged by size: 4642 1366 1208 PfoI T'CCnGG_A Cuts at: 2529 3072 3098 4116 2529 Size: 543 26 1018 5629 Fragments arranged by size: 5629 1018 543 26 PmeI GTTT'AAAC Cuts at: 4520 4520 Size: 7216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5285 5317 6798 6830 5285 Size: 32 1481 32 5671 Fragments arranged by size: 5671 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7216 PsiI TTA'TAA Cuts at: 4786 4786 Size: 7216 PspOMI G'GGCC_C Cuts at: 2406 3125 2406 Size: 719 6497 PstI C_TGCA'G Cuts at: 830 2342 2808 3270 3492 3555 3863 4101 4182 Size: 1512 466 462 222 63 308 238 81 Cuts at: 4182 4568 830 Size: 386 3478 Fragments arranged by size: 3478 1512 466 462 386 308 238 222 81 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7216 PvuII CAG'CTG Cuts at: 2782 3023 2782 Size: 241 6975 SacI G_AGCT'C Cuts at: 2985 2985 Size: 7216 SacII CC_GC'GG Cuts at: 3670 3670 Size: 7216 SalI G'TCGA_C Cuts at: 1936 4558 1936 Size: 2622 4594 SapI GCTCTTCn'nnn_ Cuts at: 2338 4124 2338 Size: 1786 5430 SbfI CC_TGCA'GG Cuts at: 3270 3863 4568 3270 Size: 593 705 5918 Fragments arranged by size: 5918 705 593 ScaI AGT'ACT Cuts at: 5464 7176 5464 Size: 1712 5504 SfcI C'TryA_G Cuts at: 826 1045 2338 2804 3202 3266 3361 3488 3529 Size: 219 1293 466 398 64 95 127 41 Cuts at: 3529 3551 3859 4097 4178 4564 5699 6380 6571 Size: 22 308 238 81 386 1135 681 191 Cuts at: 6571 826 Size: 1471 Fragments arranged by size: 1471 1293 1135 681 466 398 386 308 238 219 191 127 95 81 64 41 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 SgrAI Cr'CCGG_yG Cuts at: 1700 2203 1700 Size: 503 6713 SmaI CCC'GGG Cuts at: 1872 4290 4543 1872 Size: 2418 253 4545 Fragments arranged by size: 4545 2418 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 3599 4139 4404 5320 6221 6483 Size: 197 926 1656 540 265 916 901 262 Cuts at: 6483 820 Size: 1553 Fragments arranged by size: 1656 1553 926 916 901 540 265 262 197 SnaBI TAC'GTA Cuts at: 493 5007 493 Size: 4514 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7216 SphI G_CATG'C Cuts at: 1132 4574 1132 Size: 3442 3774 SspI AAT'ATT Cuts at: 5 52 5140 5 Size: 47 5088 2081 Fragments arranged by size: 5088 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7216 StyI C'CwwG_G Cuts at: 513 1065 2004 2695 3348 3618 3636 4655 513 Size: 552 939 691 653 270 18 1019 3074 Fragments arranged by size: 3074 1019 939 691 653 552 270 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4037 5243 5260 5413 5598 6043 325 Size: 1255 2457 1206 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 1206 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2925 3330 3585 Size: 274 80 33 51 1443 948 405 255 Cuts at: 3585 3891 5462 7174 96 Size: 306 1571 1712 138 Fragments arranged by size: 1712 1571 1443 948 405 306 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3068 3100 7176 7208 3068 Size: 32 4076 32 3076 Fragments arranged by size: 4076 3076 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2230 1316 Size: 914 6302 XbaI T'CTAG_A Cuts at: 4552 4552 Size: 7216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 4114 2845 Size: 1269 5947 XhoI C'TCGA_G Cuts at: 1943 4404 1943 Size: 2461 4755 XmaI C'CCGG_G Cuts at: 1870 4288 4541 1870 Size: 2418 253 4545 Fragments arranged by size: 4545 2418 253 XmnI GAAnn'nnTTC Cuts at: 4133 4157 5345 4133 Size: 24 1188 6004 Fragments arranged by size: 6004 1188 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2680 5024 276 Size: 53 83 186 936 1146 2344 2468 Fragments arranged by size: 2468 2344 1146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI