(Circular) (Six-base) MAPSORT of: pFN21AB9454.seq Check: 9407 from: 1 to: 6262 pFN21AB9454 6262 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 3605 1937 Size: 118 1550 4594 Fragments arranged by size: 4594 1550 118 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2802 4325 5709 5977 1632 Size: 464 706 1523 1384 268 1917 Fragments arranged by size: 1917 1523 1384 706 464 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 3074 5161 6138 1203 Size: 1871 2087 977 1327 Fragments arranged by size: 2087 1871 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 3029 1166 Size: 1863 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3329 3406 1184 Size: 2145 77 4040 Fragments arranged by size: 4040 2145 77 AlwNI CAG_nnn'CTG Cuts at: 4005 5577 4005 Size: 1572 4690 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2455 3571 3797 3949 6058 1493 Size: 962 1116 226 152 2109 1697 Fragments arranged by size: 2109 1697 1116 962 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 3587 1870 Size: 73 1644 4545 Fragments arranged by size: 4545 1644 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3583 5038 618 Size: 325 217 627 1796 1455 1842 Fragments arranged by size: 1842 1796 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2916 3367 3581 1391 Size: 1525 451 214 4072 Fragments arranged by size: 4072 1525 451 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 3071 928 Size: 971 216 956 4119 Fragments arranged by size: 4119 971 956 216 BbvCI CC'TCA_GC Cuts at: 2790 2790 Size: 6262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3310 3475 6257 3310 Size: 165 2782 3315 Fragments arranged by size: 3315 2782 165 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 4323 5479 1790 Size: 2533 1156 2573 Fragments arranged by size: 2573 2533 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2726 2960 3993 Size: 416 53 83 399 24 1309 234 1033 Cuts at: 3993 4951 442 Size: 958 1753 Fragments arranged by size: 1753 1309 1033 958 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 2781 4922 1239 Size: 1380 162 2141 2579 Fragments arranged by size: 2579 2141 1380 162 Bpu10I CC'TnA_GC Cuts at: 2657 2790 2657 Size: 133 6129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3093 3130 4387 5252 5550 3093 Size: 37 1257 865 298 3805 Fragments arranged by size: 3805 1257 865 298 37 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2826 4925 882 Size: 617 1327 2099 2219 Fragments arranged by size: 2219 2099 1327 617 BsaAI yAC'GTr Cuts at: 493 1204 3235 4053 493 Size: 711 2031 818 2702 Fragments arranged by size: 2702 2031 818 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4069 4451 5086 275 Size: 53 83 186 936 2536 382 635 1451 Fragments arranged by size: 2536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 4695 5093 5367 5514 5925 2011 Size: 2684 398 274 147 411 2348 Fragments arranged by size: 2684 2348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2592 2622 2754 2784 2823 Size: 30 165 30 1189 30 132 30 39 Cuts at: 2823 2853 1178 Size: 30 4587 Fragments arranged by size: 4587 1189 165 132 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2341 2359 2717 1186 Size: 1155 18 358 4731 Fragments arranged by size: 4731 1155 358 18 BseYI C'CCAG_C Cuts at: 3499 5465 5961 3499 Size: 1966 496 3800 Fragments arranged by size: 3800 1966 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2691 1163 Size: 573 955 4734 Fragments arranged by size: 4734 955 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2775 3367 4323 4408 5479 2775 Size: 592 956 85 1071 3558 Fragments arranged by size: 3558 1071 956 592 85 BsmI GAATG_Cn' Cuts at: 3008 3773 3866 3008 Size: 765 93 5404 Fragments arranged by size: 5404 765 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2775 2916 3367 3581 4323 4408 5479 Size: 399 985 141 451 214 742 85 1071 Cuts at: 5479 1391 Size: 2174 Fragments arranged by size: 2174 1071 985 742 451 399 214 141 85 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 3617 844 Size: 1819 954 3489 Fragments arranged by size: 3489 1819 954 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5093 1700 Size: 110 3096 187 2869 Fragments arranged by size: 3096 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2512 96 Size: 2416 3846 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstYI r'GATC_y Cuts at: 1972 3310 3475 3592 3959 4344 4361 5813 5899 Size: 1338 165 117 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1338 385 367 346 165 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5710 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2887 487 Size: 683 56 1661 3862 Fragments arranged by size: 3862 1661 683 56 BtsI GCAGTG_nn' Cuts at: 3514 3778 4572 4592 3514 Size: 264 794 20 5184 Fragments arranged by size: 5184 794 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 2095 3566 2095 Size: 1471 4791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3467 5269 809 Size: 507 2151 1802 1802 Fragments arranged by size: 2151 1802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3709 4598 6227 Size: 54 1425 50 275 18 1879 889 1629 Cuts at: 6227 8 Size: 43 Fragments arranged by size: 1879 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2341 2359 2368 2377 2386 2392 2410 2588 Size: 988 18 9 9 9 6 18 178 Cuts at: 2588 4192 1353 Size: 1604 3423 Fragments arranged by size: 3423 1604 988 178 18 18 9 9 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2218 4849 5223 5369 387 Size: 1064 138 629 2631 374 146 1280 Fragments arranged by size: 2631 1280 1064 629 374 146 138 EcoICRI GAG'CTC Cuts at: 3365 3365 Size: 6262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2619 2781 2802 4325 4922 5709 Size: 393 464 523 162 21 1523 597 787 Cuts at: 5709 5977 1239 Size: 268 1524 Fragments arranged by size: 1524 1523 787 597 523 464 393 268 162 21 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 5409 1678 Size: 3731 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2318 2350 2823 Size: 32 135 32 549 32 165 32 473 Cuts at: 2823 2855 4903 4935 4977 5009 1373 Size: 32 2048 32 42 32 2626 Fragments arranged by size: 2626 2048 549 473 165 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5376 5560 1305 Size: 4071 184 2007 Fragments arranged by size: 4071 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2103 3029 4220 4579 4738 518 Size: 648 937 926 1191 359 159 2042 Fragments arranged by size: 2042 1191 937 926 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3678 4355 5503 5748 1606 Size: 2072 677 1148 245 2120 Fragments arranged by size: 2120 2072 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5710 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3078 3620 5165 1132 Size: 97 1849 542 1545 2229 Fragments arranged by size: 2229 1849 1545 542 97 PasI CC'CwG_GG Cuts at: 1348 3535 1348 Size: 2187 4075 PciI A'CATG_T Cuts at: 3074 5161 3074 Size: 2087 4175 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PfoI T'CCnGG_A Cuts at: 2596 2596 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2051 3335 1613 Size: 438 1284 4540 Fragments arranged by size: 4540 1284 438 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2415 2447 2415 Size: 32 6230 PstI C_TGCA'G Cuts at: 830 3614 830 Size: 2784 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 SacI G_AGCT'C Cuts at: 3367 3367 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SfcI C'TryA_G Cuts at: 826 1045 2849 3043 3610 4745 5426 5617 826 Size: 219 1804 194 567 1135 681 191 1471 Fragments arranged by size: 1804 1471 1135 681 567 219 194 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3108 3145 4366 5267 5529 820 Size: 197 926 1165 37 1221 901 262 1553 Fragments arranged by size: 1553 1221 1165 926 901 262 197 37 SnaBI TAC'GTA Cuts at: 493 3235 4053 493 Size: 2742 818 2702 Fragments arranged by size: 2742 2702 818 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 2820 1517 Size: 1303 4959 StyI C'CwwG_G Cuts at: 513 1065 2284 3701 513 Size: 552 1219 1417 3074 Fragments arranged by size: 3074 1417 1219 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 2078 2858 3225 3491 4289 4306 Size: 1255 447 51 780 367 266 798 17 Cuts at: 4306 4459 4644 5089 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 798 780 447 445 367 266 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2512 3511 4508 Size: 274 80 33 51 1443 535 999 997 Cuts at: 4508 6220 96 Size: 1712 138 Fragments arranged by size: 1712 1443 999 997 535 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2653 2685 2754 2786 6222 6254 2653 Size: 32 69 32 3436 32 2661 Fragments arranged by size: 3436 2661 69 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2837 1316 Size: 1521 4741 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3532 3532 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 2553 4391 2553 Size: 1838 4424 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI