Restriction map for
pFN21AB9449
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AclI AcsI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BbrPI BbsI
BbvCI BciVI BclI BfaI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsiWI BsmAI BsmBI Bsp1407I
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MboII MfeI MluI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 702 CTTAAG C^TTAAG
AflIII 950 ACGTGT A^C[GA][CT]GT
AgeI 1357 ACCGGT A^CCGGT
AlwNI 980 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 793 GGGCCC GGGCC^C
AspI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 311 TGCGCA TGC^GCA
BalI 302 TGGCCA TGG^CCA
BamHI 491 GGATCC G^GATCC
BcgI 1017 CGATGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 702 CTTAAG C^TTAAG
BsaBI 601 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1326 GCATTC GAATGC(1/-1)
BspDI 916 ATCGAT AT^CGAT
BspMI 170 ACCTGC ACCTGC(4/8)
BstEII 849 GGTGACC G^GT[ACGT]ACC
BstPI 849 GGTGACC G^GT[ACGT]ACC
BstXI 1013 CCAACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 916 ATCGAT AT^CGAT
DraII 792 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 792 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 849 GGTGACC G^GT[ACGT]ACC
FseI 41 GGCCGGCC GGCCGG^CC
FspI 311 TGCGCA TGC^GCA
HgaI 560 GCGTC GACGC(5/10)
KspI 37 CCGCGG CCGC^GG
MamI 601 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 302 TGGCCA TGG^CCA
MscI 302 TGGCCA TGG^CCA
NaeI 42 GCCGGC GCC^GGC
NgoMIV 42 GCCGGC G^CCGGC
PflFI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1357 ACCGGT A^CCGGT
PvuII 983 CAGCTG CAG^CTG
SacII 37 CCGCGG CCGC^GG
SbfI 171 CCTGCAGG CCTGCA^GG
SmlI 702 CTTAAG C^T[CT][GA]AG
Sse8387I 171 CCTGCAGG CCTGCA^GG
TfiI 634 GAATC G^A[AT]TC
Tth111I 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 797 CCAACTTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 630 TCCGGA T^CCGGA
637 TCCGGA
AcyI 65 GGCGCC G[GA]^CG[CT]C
1341 GGCGCC
BanI 65 GGCGCC G^G[CT][GA]CC
1341 GGCGCC
BbeI 65 GGCGCC GGCGC^C
1341 GGCGCC
BglI 17 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
690 GCCCAACGGGC
BpmI 817 CTGGAG CTGGAG(16/14)
1139 CTGGAG
BsaHI 65 GGCGCC G[GA]^CG[CT]C
1341 GGCGCC
BseAI 630 TCCGGA T^CCGGA
637 TCCGGA
BsgI 1180 CTGCAC GTGCAG(16/14)
1282 GTGCAG
BsiEI 40 CGGCCG CG[GA][CT]^CG
44 CGGCCG
BspEI 630 TCCGGA T^CCGGA
637 TCCGGA
BsrBI 232 GAGCGG CCGCTC(-3/-3)
517 GAGCGG
BsrFI 42 GCCGGC [GA]^CCGG[CT]
1357 ACCGGT
BsrI 597 ACTGG ACTGG(1/-1)
996 CCAGT
Cfr10I 42 GCCGGC [GA]^CCGG[CT]
1357 ACCGGT
DdeI 536 CTCAG C^T[ACGT]AG
1085 CTCAG
DsaI 37 CCGCGG C^C[GA][CT]GG
1254 CCACGG
EagI 40 CGGCCG C^GGCCG
44 CGGCCG
EclXI 40 CGGCCG C^GGCCG
44 CGGCCG
Eco52I 40 CGGCCG C^GGCCG
44 CGGCCG
EcoNI 93 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
432 CCTGCGCCAGG
Hin1I 65 GGCGCC G[GA]^CG[CT]C
1341 GGCGCC
KasI 65 GGCGCC G^GCGCC
1341 GGCGCC
MroI 630 TCCGGA T^CCGGA
637 TCCGGA
MseI 485 TTAA T^TAA
703 TTAA
NarI 65 GGCGCC GG^CGCC
1341 GGCGCC
PflMI 979 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1001 CCAGCGGGTGG
PstI 172 CTGCAG CTGCA^G
1068 CTGCAG
ScaI 191 AGTACT AGT^ACT
505 AGTACT
SfcI 172 CTGCAG C^T[GA][CT]AG
1068 CTGCAG
SfoI 65 GGCGCC GGC^GCC
1341 GGCGCC
Tru9I 485 TTAA T^TAA
703 TTAA
Van91I 979 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1001 CCAGCGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SmaI TaqI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI