(Circular) (Six-base) MAPSORT of: pFN21AB9449.seq Check: 2784 from: 1 to: 6100 pFN21AB9449 6100 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3296 4163 5547 5815 1632 Size: 1664 867 1384 268 1917 Fragments arranged by size: 1917 1664 1384 867 268 AflII C'TTAA_G Cuts at: 820 1017 2707 820 Size: 197 1690 4213 Fragments arranged by size: 4213 1690 197 AflIII A'CryG_T Cuts at: 1203 2955 4999 5976 1203 Size: 1752 2044 977 1327 Fragments arranged by size: 2044 1752 1327 977 AgeI A'CCGG_T Cuts at: 3362 4931 3362 Size: 1569 4531 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2691 1184 Size: 1507 4593 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2962 2994 2962 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 2990 3843 5415 2990 Size: 853 1572 3675 Fragments arranged by size: 3675 1572 853 ApaI G_GGCC'C Cuts at: 2802 2802 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2273 2333 2966 3165 3310 3383 Size: 73 123 207 60 633 199 145 73 Cuts at: 3383 3425 1870 Size: 42 4545 Fragments arranged by size: 4545 633 207 199 145 123 73 73 60 42 BamHI G'GATC_C Cuts at: 2496 3430 3797 2496 Size: 934 367 4799 Fragments arranged by size: 4799 934 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 3346 3421 4876 618 Size: 325 217 627 283 1276 75 1455 1842 Fragments arranged by size: 1842 1455 1276 627 325 283 217 75 BanII G_rGCy'C Cuts at: 1391 2802 2945 3164 3224 3419 1391 Size: 1411 143 219 60 195 4072 Fragments arranged by size: 4072 1411 219 195 143 60 BbeI G_GCGC'C Cuts at: 2074 3350 2074 Size: 1276 4824 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3011 3045 4291 4325 1903 Size: 34 50 34 990 34 1246 34 3678 Fragments arranged by size: 3678 1246 990 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 2701 4711 Size: 107 122 71 631 605 356 673 2010 Cuts at: 4711 136 Size: 1525 Fragments arranged by size: 2010 1525 673 631 605 356 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2706 2802 3082 4161 5317 1790 Size: 916 96 280 1079 1156 2573 Fragments arranged by size: 2573 1156 1079 916 280 96 BmgBI CAC'GTC Cuts at: 1318 2146 2956 3201 1318 Size: 828 810 245 4217 Fragments arranged by size: 4217 828 810 245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2843 3165 4760 1239 Size: 1604 322 1595 2579 Fragments arranged by size: 2579 1604 1595 322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 2610 6094 2610 Size: 3484 2616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2071 3347 3907 4289 Size: 53 83 186 936 538 1276 560 382 Cuts at: 4289 4924 275 Size: 635 1451 Fragments arranged by size: 1451 1276 936 635 560 538 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2635 2642 3323 3362 4533 4931 5205 5352 5763 Size: 7 681 39 1171 398 274 147 411 Cuts at: 5763 2635 Size: 2972 Fragments arranged by size: 2972 1171 681 411 398 274 147 39 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2869 2899 3179 3209 1178 Size: 30 165 30 1466 30 280 30 4069 Fragments arranged by size: 4069 1466 280 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 3076 3342 1186 Size: 1063 827 266 3944 Fragments arranged by size: 3944 1063 827 266 BseYI C'CCAG_C Cuts at: 2074 2260 2831 2983 5303 5799 2074 Size: 186 571 152 2320 496 2375 Fragments arranged by size: 2375 2320 571 496 186 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3170 3308 1163 Size: 573 1434 138 3955 Fragments arranged by size: 3955 1434 573 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2052 3550 4311 5339 1540 Size: 275 186 47 4 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 47 4 BsiHKAI G_wGCw'C Cuts at: 2394 2549 3302 4161 4246 5317 2394 Size: 155 753 859 85 1071 3177 Fragments arranged by size: 3177 1071 859 753 155 85 BsmI GAATG_Cn' Cuts at: 3331 3611 3704 3331 Size: 280 93 5727 Fragments arranged by size: 5727 280 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2549 2706 2802 2945 3082 3164 Size: 399 604 155 157 96 143 137 82 Cuts at: 3164 3224 3302 3419 4161 4246 5317 1391 Size: 60 78 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 157 155 143 137 117 96 85 82 78 60 BspEI T'CCGG_A Cuts at: 2635 2642 5763 2635 Size: 7 3121 2972 Fragments arranged by size: 3121 2972 7 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 3455 844 Size: 1340 1271 3489 Fragments arranged by size: 3489 1340 1271 BsrBI CCG'CTC Cuts at: 1394 1823 2239 2524 3987 1394 Size: 429 416 285 1463 3507 Fragments arranged by size: 3507 1463 429 416 285 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 3362 4744 4931 1700 Size: 110 237 1315 1382 187 2869 Fragments arranged by size: 2869 1382 1315 237 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 2854 1184 Size: 1670 4430 BstXI CCAn_nnnn'nTGG Cuts at: 3025 3025 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2418 2496 2605 3430 3797 4182 4199 5651 Size: 446 78 109 825 367 385 17 1452 Cuts at: 5651 5737 5749 6095 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 825 446 385 367 346 109 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2042 3259 513 Size: 552 977 1217 3354 Fragments arranged by size: 3354 1217 977 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 2132 2690 3082 3616 4410 4430 2132 Size: 558 392 534 794 20 3802 Fragments arranged by size: 3802 794 558 534 392 20 ClaI AT'CG_AT Cuts at: 1554 2922 1554 Size: 1368 4732 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2049 2307 Size: 54 1425 50 275 18 215 4 258 Cuts at: 2307 3547 4436 6065 8 Size: 1240 889 1629 43 Fragments arranged by size: 1629 1425 1240 889 275 258 215 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2045 2049 3547 1537 Size: 275 233 4 1498 4090 Fragments arranged by size: 4090 1498 275 233 4 EarI CTCTTCn'nnn_ Cuts at: 1353 4030 1353 Size: 2677 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2440 2831 4687 5061 5207 387 Size: 1064 138 851 391 1856 374 146 1280 Fragments arranged by size: 1856 1280 1064 851 391 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2843 3165 3296 4163 4760 5547 5815 Size: 393 1211 322 131 867 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 1211 867 787 597 393 322 268 131 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2441 2102 Size: 339 5761 EcoO109I rG'GnC_Cy Cuts at: 2798 2798 Size: 6100 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 6100 FseI GG_CCGG'CC Cuts at: 2051 2051 Size: 6100 FspI TGC'GCA Cuts at: 2318 4606 2318 Size: 2288 3812 HaeII r_GCGC'y Cuts at: 1678 2074 2911 3350 5247 1678 Size: 396 837 439 1897 2531 Fragments arranged by size: 2531 1897 837 439 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2577 2609 4741 4773 4815 Size: 32 135 32 1005 32 2132 32 42 Cuts at: 4815 4847 1373 Size: 32 2626 Fragments arranged by size: 2626 2132 1005 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KasI G'GCGC_C Cuts at: 2070 3346 2070 Size: 1276 4824 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 5214 5398 1305 Size: 1116 2793 184 2007 Fragments arranged by size: 2793 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 10 Size: 54 1425 343 477 3801 Fragments arranged by size: 3801 1425 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2990 3009 3130 3516 4193 5341 5586 Size: 438 946 19 121 386 677 1148 245 Cuts at: 5586 1606 Size: 2120 Fragments arranged by size: 2120 1148 946 677 438 386 245 121 19 NaeI GCC'GGC Cuts at: 2049 2049 Size: 6100 NarI GG'CG_CC Cuts at: 2071 3347 2071 Size: 1276 4824 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5548 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NgoMIV G'CCGG_C Cuts at: 2047 2047 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3458 5003 1132 Size: 97 2229 1545 2229 Fragments arranged by size: 2229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2860 1348 Size: 1512 4588 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2990 3012 1108 Size: 1882 22 4196 Fragments arranged by size: 4196 1882 22 PfoI T'CCnGG_A Cuts at: 2715 2715 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 2798 2798 Size: 6100 PstI C_TGCA'G Cuts at: 830 2181 3077 3452 830 Size: 1351 896 375 3478 Fragments arranged by size: 3478 1351 896 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2990 2990 Size: 6100 SacII CC_GC'GG Cuts at: 2045 2045 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 2181 3452 2181 Size: 1271 4829 ScaI AGT'ACT Cuts at: 2198 2512 4348 6060 2198 Size: 314 1836 1712 2238 Fragments arranged by size: 2238 1836 1712 314 SexAI A'CCwGG_T Cuts at: 2165 2469 2850 2165 Size: 304 381 5415 Fragments arranged by size: 5415 381 304 SfcI C'TryA_G Cuts at: 826 1045 2177 3073 3448 4583 5264 5455 826 Size: 219 1132 896 375 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 896 681 375 219 191 SfoI GGC'GCC Cuts at: 2072 3348 2072 Size: 1276 4824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2068 2968 3167 3385 3427 1872 Size: 196 900 199 218 42 4545 Fragments arranged by size: 4545 900 218 199 196 42 SmlI C'TyrA_G Cuts at: 820 1017 1943 2707 4204 5105 5367 820 Size: 197 926 764 1497 901 262 1553 Fragments arranged by size: 1553 1497 926 901 764 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SrfI GCCC'GGGC Cuts at: 2068 2068 Size: 6100 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 3539 513 Size: 552 2474 3074 Fragments arranged by size: 3074 2474 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 3134 4127 4144 4297 4482 4927 Size: 1255 537 1017 993 17 153 185 445 Cuts at: 4927 325 Size: 1498 Fragments arranged by size: 1498 1255 1017 993 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2196 2510 Size: 274 80 33 51 1443 105 114 314 Cuts at: 2510 4346 6058 96 Size: 1836 1712 138 Fragments arranged by size: 1836 1712 1443 314 274 138 114 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2869 2901 3179 3211 6060 6092 2869 Size: 32 278 32 2849 32 2877 Fragments arranged by size: 2877 2849 278 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2471 1316 Size: 1155 4945 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2809 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 2066 2966 3165 3383 3425 1870 Size: 196 900 199 218 42 4545 Fragments arranged by size: 4545 900 218 199 196 42 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SwaI