Restriction map for
pFN21AB9449
(Linear) (Six-base) MAPSORT of: KIBB9449.seq Check: 9787 from: 1 to: 1395
KIBB9449.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 1395
Size: 1291 104
AflII C'TTAA_G
Cuts at: 0 702 1395
Size: 702 693
AflIII A'CryG_T
Cuts at: 0 950 1395
Size: 950 445
AgeI A'CCGG_T
Cuts at: 0 1357 1395
Size: 1357 38
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 686 1395
Size: 686 709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 957 989 1395
Size: 957 32 406
Fragments arranged by size:
957 406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 985 1395
Size: 985 410
ApaI G_GGCC'C
Cuts at: 0 797 1395
Size: 797 598
AvaI C'yCGr_G
Cuts at: 0 61 268 328 961 1160 1305 1378 1395
Size: 61 207 60 633 199 145 73 17
Fragments arranged by size:
633 207 199 145 73 61 60 17
BamHI G'GATC_C
Cuts at: 0 491 1395
Size: 491 904
BanI G'GyrC_C
Cuts at: 0 65 1341 1395
Size: 65 1276 54
Fragments arranged by size:
1276 65 54
BanII G_rGCy'C
Cuts at: 0 797 940 1159 1219 1395
Size: 797 143 219 60 176
Fragments arranged by size:
797 219 176 143 60
BbeI G_GCGC'C
Cuts at: 0 69 1345 1395
Size: 69 1276 50
Fragments arranged by size:
1276 69 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 1040 1395
Size: 1006 34 355
Fragments arranged by size:
1006 355 34
BglI GCCn_nnn'nGGC
Cuts at: 0 23 696 1395
Size: 23 673 699
Fragments arranged by size:
699 673 23
Bme1580I G_kGCm'C
Cuts at: 0 701 797 1077 1395
Size: 701 96 280 318
Fragments arranged by size:
701 318 280 96
BmgBI CAC'GTC
Cuts at: 0 141 951 1196 1395
Size: 141 810 245 199
Fragments arranged by size:
810 245 199 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1160 1395
Size: 838 322 235
Fragments arranged by size:
838 322 235
BsaBI GATnn'nnATC
Cuts at: 0 605 1395
Size: 605 790
BsaHI Gr'CG_yC
Cuts at: 0 66 1342 1395
Size: 66 1276 53
Fragments arranged by size:
1276 66 53
BsaWI w'CCGG_w
Cuts at: 0 630 637 1318 1357 1395
Size: 630 7 681 39 38
Fragments arranged by size:
681 630 39 38 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 864 894 1174 1204 1395
Size: 864 30 280 30 191
Fragments arranged by size:
864 280 191 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 1071 1337 1395
Size: 244 827 266 58
Fragments arranged by size:
827 266 244 58
BseYI C'CCAG_C
Cuts at: 0 69 255 826 978 1395
Size: 69 186 571 152 417
Fragments arranged by size:
571 417 186 152 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1303 1395
Size: 1165 138 92
Fragments arranged by size:
1165 138 92
BsiEI CG_ry'CG
Cuts at: 0 43 47 1395
Size: 43 4 1348
Fragments arranged by size:
1348 43 4
BsiHKAI G_wGCw'C
Cuts at: 0 389 544 1297 1395
Size: 389 155 753 98
Fragments arranged by size:
753 389 155 98
BsmI GAATG_Cn'
Cuts at: 0 1326 1395
Size: 1326 69
Bsp1286I G_dGCh'C
Cuts at: 0 389 544 701 797 940 1077 1159 1219
Size: 389 155 157 96 143 137 82 60
Cuts at: 1219 1297 1395
Size: 78 98
Fragments arranged by size:
389 157 155 143 137 98 96 82
78 60
BspEI T'CCGG_A
Cuts at: 0 630 637 1395
Size: 630 7 758
Fragments arranged by size:
758 630 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 1395
Size: 179 1216
BsrBI CCG'CTC
Cuts at: 0 234 519 1395
Size: 234 285 876
Fragments arranged by size:
876 285 234
BsrFI r'CCGG_y
Cuts at: 0 42 1357 1395
Size: 42 1315 38
Fragments arranged by size:
1315 42 38
BstEII G'GTnAC_C
Cuts at: 0 849 1395
Size: 849 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1020 1395
Size: 1020 375
BstYI r'GATC_y
Cuts at: 0 413 491 600 1395
Size: 413 78 109 795
Fragments arranged by size:
795 413 109 78
BtgI C'CryG_G
Cuts at: 0 37 1254 1395
Size: 37 1217 141
Fragments arranged by size:
1217 141 37
BtsI GCAGTG_nn'
Cuts at: 0 127 685 1077 1395
Size: 127 558 392 318
Fragments arranged by size:
558 392 318 127
ClaI AT'CG_AT
Cuts at: 0 917 1395
Size: 917 478
EaeI y'GGCC_r
Cuts at: 0 40 44 302 1395
Size: 40 4 258 1093
Fragments arranged by size:
1093 258 40 4
EagI C'GGCC_G
Cuts at: 0 40 44 1395
Size: 40 4 1351
Fragments arranged by size:
1351 40 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 435 826 1395
Size: 435 391 569
Fragments arranged by size:
569 435 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1160 1291 1395
Size: 838 322 131 104
Fragments arranged by size:
838 322 131 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 436 1395
Size: 97 339 959
Fragments arranged by size:
959 339 97
EcoO109I rG'GnC_Cy
Cuts at: 0 793 1395
Size: 793 602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 1395
Size: 1080 315
FseI GG_CCGG'CC
Cuts at: 0 46 1395
Size: 46 1349
FspI TGC'GCA
Cuts at: 0 313 1395
Size: 313 1082
HaeII r_GCGC'y
Cuts at: 0 69 906 1345 1395
Size: 69 837 439 50
Fragments arranged by size:
837 439 69 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1395
Size: 572 32 791
Fragments arranged by size:
791 572 32
KasI G'GCGC_C
Cuts at: 0 65 1341 1395
Size: 65 1276 54
Fragments arranged by size:
1276 65 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1395
Size: 416 979
MscI TGG'CCA
Cuts at: 0 304 1395
Size: 304 1091
MspA1I CmG'CkG
Cuts at: 0 39 985 1004 1125 1395
Size: 39 946 19 121 270
Fragments arranged by size:
946 270 121 39 19
NaeI GCC'GGC
Cuts at: 0 44 1395
Size: 44 1351
NarI GG'CG_CC
Cuts at: 0 66 1342 1395
Size: 66 1276 53
Fragments arranged by size:
1276 66 53
NgoMIV G'CCGG_C
Cuts at: 0 42 1395
Size: 42 1353
PasI CC'CwG_GG
Cuts at: 0 855 1395
Size: 855 540
PflMI CCAn_nnn'nTGG
Cuts at: 0 985 1007 1395
Size: 985 22 388
Fragments arranged by size:
985 388 22
PfoI T'CCnGG_A
Cuts at: 0 710 1395
Size: 710 685
PspOMI G'GGCC_C
Cuts at: 0 793 1395
Size: 793 602
PstI C_TGCA'G
Cuts at: 0 176 1072 1395
Size: 176 896 323
Fragments arranged by size:
896 323 176
PvuII CAG'CTG
Cuts at: 0 985 1395
Size: 985 410
SacII CC_GC'GG
Cuts at: 0 40 1395
Size: 40 1355
SbfI CC_TGCA'GG
Cuts at: 0 176 1395
Size: 176 1219
ScaI AGT'ACT
Cuts at: 0 193 507 1395
Size: 193 314 888
Fragments arranged by size:
888 314 193
SexAI A'CCwGG_T
Cuts at: 0 160 464 845 1395
Size: 160 304 381 550
Fragments arranged by size:
550 381 304 160
SfcI C'TryA_G
Cuts at: 0 172 1068 1395
Size: 172 896 327
Fragments arranged by size:
896 327 172
SfoI GGC'GCC
Cuts at: 0 67 1343 1395
Size: 67 1276 52
Fragments arranged by size:
1276 67 52
SmaI CCC'GGG
Cuts at: 0 63 963 1162 1380 1395
Size: 63 900 199 218 15
Fragments arranged by size:
900 218 199 63 15
SmlI C'TyrA_G
Cuts at: 0 702 1395
Size: 702 693
SrfI GCCC'GGGC
Cuts at: 0 63 1395
Size: 63 1332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 1129 1395
Size: 112 1017 266
Fragments arranged by size:
1017 266 112
TatI w'GTAC_w
Cuts at: 0 77 191 505 1395
Size: 77 114 314 890
Fragments arranged by size:
890 314 114 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 864 896 1174 1206 1395
Size: 864 32 278 32 189
Fragments arranged by size:
864 278 189 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 466 1395
Size: 466 929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 1395
Size: 804 591
XmaI C'CCGG_G
Cuts at: 0 61 961 1160 1378 1395
Size: 61 900 199 218 17
Fragments arranged by size:
900 218 199 61 17
Enzymes that do cut:
AcuI AflII AflIII AgeI AlfI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BcgI BglI Bme1580I
BmgBI BpmI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BstEII BstXI BstYI BtgI BtsI ClaI EaeI
EagI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI
HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI
NgoMIV PasI PflMI PfoI PspOMI PstI PvuII SacII
SbfI ScaI SexAI SfcI SfoI SmaI SmlI SrfI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsiWI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI
EcoRV FspAI HincII HindIII HpaI KpnI MfeI MluI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI XbaI XhoI XmnI ZraI