(Circular) (Six-base) MAPSORT of: pFN21AB9433.seq Check: 8808 from: 1 to: 5521 pFN21AB9433 5521 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2629 3584 4968 5236 1632 Size: 815 182 955 1384 268 1917 Fragments arranged by size: 1917 1384 955 815 268 182 AfeI AGC'GCT Cuts at: 2231 2231 Size: 5521 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 2524 4420 5397 1203 Size: 1321 1896 977 1327 Fragments arranged by size: 1896 1327 1321 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 2062 2382 4250 2062 Size: 320 1868 3333 Fragments arranged by size: 3333 1868 320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AlwNI CAG_nnn'CTG Cuts at: 2494 2536 2707 3264 4836 2494 Size: 42 171 557 1572 3179 Fragments arranged by size: 3179 1572 557 171 42 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2123 2846 1870 Size: 73 89 91 723 4545 Fragments arranged by size: 4545 723 91 89 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2842 4297 618 Size: 325 217 627 325 730 1455 1842 Fragments arranged by size: 1842 1455 730 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 2840 1391 Size: 1449 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2799 928 Size: 971 900 3650 Fragments arranged by size: 3650 971 900 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 4132 136 Size: 107 122 71 631 605 1126 1334 1525 Fragments arranged by size: 1525 1334 1126 631 605 122 107 71 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2129 2600 2609 2938 2129 Size: 471 9 329 4712 Fragments arranged by size: 4712 471 329 9 Bme1580I G_kGCm'C Cuts at: 1790 3582 4738 1790 Size: 1792 1156 2573 Fragments arranged by size: 2573 1792 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2759 2797 2740 Size: 19 38 5464 Fragments arranged by size: 5464 38 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 4181 1239 Size: 1235 1707 2579 Fragments arranged by size: 2579 1707 1235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4511 4809 3646 Size: 865 298 4358 Fragments arranged by size: 4358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4184 882 Size: 617 2685 2219 Fragments arranged by size: 2685 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2443 3312 493 Size: 711 1239 869 2702 Fragments arranged by size: 2702 1239 869 711 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2801 3328 3710 4345 Size: 53 83 186 936 1268 527 382 635 Cuts at: 4345 275 Size: 1451 Fragments arranged by size: 1451 1268 936 635 527 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2104 3954 4352 4626 4773 5184 2104 Size: 1850 398 274 147 411 2441 Fragments arranged by size: 2441 1850 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2140 2346 2558 2748 1186 Size: 838 3 113 206 212 190 3959 Fragments arranged by size: 3959 838 212 206 190 113 3 BseYI C'CCAG_C Cuts at: 2515 4724 5220 2515 Size: 2209 496 2816 Fragments arranged by size: 2816 2209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4948 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2406 2971 3732 4760 1540 Size: 275 186 405 565 761 1028 2301 Fragments arranged by size: 2301 1028 761 565 405 275 186 BsiHKAI G_wGCw'C Cuts at: 3582 3667 4738 3582 Size: 85 1071 4365 Fragments arranged by size: 4365 1071 85 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 2036 Size: 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2840 3582 3667 4738 1391 Size: 399 1050 742 85 1071 2174 Fragments arranged by size: 2174 1071 1050 742 399 85 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 2715 2876 844 Size: 1406 465 161 3489 Fragments arranged by size: 3489 1406 465 161 BsrBI CCG'CTC Cuts at: 1394 1823 2613 3408 1394 Size: 429 790 795 3507 Fragments arranged by size: 3507 795 790 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2604 2660 2683 4165 4352 1700 Size: 110 794 56 23 1482 187 2869 Fragments arranged by size: 2869 1482 794 187 110 56 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 2707 794 Size: 1913 3608 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 2663 1184 Size: 1479 4042 BstYI r'GATC_y Cuts at: 1972 2083 2851 3218 3603 3620 5072 5158 5170 Size: 111 768 367 385 17 1452 86 12 Cuts at: 5170 5516 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 768 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2211 513 Size: 552 939 207 3823 Fragments arranged by size: 3823 939 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2534 3037 3831 3851 2293 Size: 241 503 794 20 3963 Fragments arranged by size: 3963 794 503 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2968 3857 Size: 54 1425 50 275 18 573 565 889 Cuts at: 3857 5486 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 573 565 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2403 2968 1537 Size: 275 591 565 4090 Fragments arranged by size: 4090 591 565 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2648 2709 3451 1353 Size: 681 614 61 742 3423 Fragments arranged by size: 3423 742 681 614 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 2175 2329 4108 4482 4628 Size: 1064 138 507 79 154 1779 374 146 Cuts at: 4628 387 Size: 1280 Fragments arranged by size: 1779 1280 1064 507 374 154 146 138 79 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2474 2629 3584 4181 4968 5236 Size: 393 815 27 155 955 597 787 268 Cuts at: 5236 1239 Size: 1524 Fragments arranged by size: 1524 955 815 787 597 393 268 155 27 EcoO109I rG'GnC_Cy Cuts at: 2141 2704 2141 Size: 563 4958 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 2233 2304 4668 1678 Size: 555 71 2364 2531 Fragments arranged by size: 2531 2364 555 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2368 2400 2750 Size: 32 135 32 476 32 288 32 350 Cuts at: 2750 2782 4162 4194 4236 4268 1373 Size: 32 1380 32 42 32 2626 Fragments arranged by size: 2626 1380 476 350 288 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 2524 5397 2524 Size: 2873 2648 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3699 Fragments arranged by size: 3699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3479 3838 3997 518 Size: 648 2313 359 159 2042 Fragments arranged by size: 2313 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2213 2352 2408 2937 3614 4762 5007 1606 Size: 607 139 56 529 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 607 529 245 139 56 NaeI GCC'GGC Cuts at: 2606 2606 Size: 5521 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NgoMIV G'CCGG_C Cuts at: 2604 2604 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2403 2968 2403 Size: 565 4956 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2088 2088 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2704 2704 Size: 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PstI C_TGCA'G Cuts at: 830 2279 2541 2873 830 Size: 1449 262 332 3478 Fragments arranged by size: 3478 1449 332 262 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 SacII CC_GC'GG Cuts at: 2214 2214 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SapI GCTCTTCn'nnn_ Cuts at: 2648 2648 Size: 5521 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2275 2537 2869 4004 4685 4876 826 Size: 219 1230 262 332 1135 681 191 1471 Fragments arranged by size: 1471 1230 1135 681 332 262 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 2660 1700 Size: 960 4561 SmaI CCC'GGG Cuts at: 1872 2034 2125 2848 1872 Size: 162 91 723 4545 Fragments arranged by size: 4545 723 162 91 SmlI C'TyrA_G Cuts at: 820 1017 1943 3625 4526 4788 820 Size: 197 926 1682 901 262 1553 Fragments arranged by size: 1682 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2960 513 Size: 552 939 956 3074 Fragments arranged by size: 3074 956 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3548 3565 3718 3903 4348 325 Size: 1255 1968 17 153 185 445 1498 Fragments arranged by size: 1968 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2032 2123 2846 1870 Size: 162 91 723 4545 Fragments arranged by size: 4545 723 162 91 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI