(Circular) (Six-base) MAPSORT of: pFN21AB9425.seq Check: 2424 from: 1 to: 6916 pFN21AB9425 6916 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2627 2627 Size: 6916 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3333 4089 4726 278 Size: 53 83 186 936 1797 756 637 2468 Fragments arranged by size: 2468 1797 936 756 637 186 83 53 AccI GT'mk_AC Cuts at: 1937 4259 1937 Size: 2322 4594 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 5043 5416 1563 Size: 3480 373 3063 Fragments arranged by size: 3480 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3323 4979 6363 6631 1632 Size: 1691 1656 1384 268 1917 Fragments arranged by size: 1917 1691 1656 1384 268 AfeI AGC'GCT Cuts at: 3503 3503 Size: 6916 AflII C'TTAA_G Cuts at: 820 1017 2027 820 Size: 197 1010 5709 Fragments arranged by size: 5709 1010 197 AflIII A'CryG_T Cuts at: 1203 5815 6792 1203 Size: 4612 977 1327 Fragments arranged by size: 4612 1327 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 2219 2621 4136 1166 Size: 1053 402 1515 3946 Fragments arranged by size: 3946 1515 1053 402 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6916 AlwNI CAG_nnn'CTG Cuts at: 4659 6231 4659 Size: 1572 5344 ApaI G_GGCC'C Cuts at: 3781 3781 Size: 6916 ApaLI G'TGCA_C Cuts at: 4973 6129 4973 Size: 1156 5760 ApoI r'AATT_y Cuts at: 1493 3512 4175 4225 4451 4603 6712 1493 Size: 2019 663 50 226 152 2109 1697 Fragments arranged by size: 2109 2019 1697 663 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 5470 160 Size: 872 4438 1606 Fragments arranged by size: 4438 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 4113 4241 1870 Size: 73 2170 128 4545 Fragments arranged by size: 4545 2170 128 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2590 2623 3389 3422 2590 Size: 33 766 33 6084 Fragments arranged by size: 6084 766 33 33 BamHI G'GATC_C Cuts at: 4246 4613 4246 Size: 367 6549 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2637 2981 4237 5692 618 Size: 325 217 627 850 344 1256 1455 1842 Fragments arranged by size: 1842 1455 1256 850 627 344 325 217 BanII G_rGCy'C Cuts at: 1391 2649 2705 3170 3781 4235 1391 Size: 1258 56 465 611 454 4072 Fragments arranged by size: 4072 1258 611 465 454 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2186 4209 928 Size: 971 287 2023 3635 Fragments arranged by size: 3635 2023 971 287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5107 5141 1903 Size: 34 3170 34 3678 Fragments arranged by size: 3678 3170 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2591 2753 4808 6018 2591 Size: 162 2055 1210 3489 Fragments arranged by size: 3489 2055 1210 162 BclI T'GATC_A Cuts at: 1548 2385 1548 Size: 837 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5527 136 Size: 107 122 71 631 605 3855 1525 Fragments arranged by size: 3855 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6911 6911 Size: 6916 BlpI GC'TnA_GC Cuts at: 3893 4333 3893 Size: 440 6476 Bme1580I G_kGCm'C Cuts at: 1790 3781 4977 6133 1790 Size: 1991 1196 1156 2573 Fragments arranged by size: 2573 1991 1196 1156 BmgBI CAC'GTC Cuts at: 1318 3352 3874 1318 Size: 2034 522 4360 Fragments arranged by size: 4360 2034 522 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3878 4030 4647 Size: 416 53 83 399 24 2461 152 617 Cuts at: 4647 5605 442 Size: 958 1753 Fragments arranged by size: 2461 1753 958 617 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 4052 5576 1239 Size: 1142 1671 1524 2579 Fragments arranged by size: 2579 1671 1524 1142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 3269 5041 5906 6204 3101 Size: 168 1772 865 298 3813 Fragments arranged by size: 3813 1772 865 298 168 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2678 2852 3474 5579 882 Size: 617 1179 174 622 2105 2219 Fragments arranged by size: 2219 2105 1179 622 617 174 BsaAI yAC'GTr Cuts at: 493 1204 4707 493 Size: 711 3503 2702 Fragments arranged by size: 3503 2702 711 BsaBI GATnn'nnATC Cuts at: 2036 6910 2036 Size: 4874 2042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3330 3984 4086 4723 Size: 53 83 186 936 1797 654 102 637 Cuts at: 4723 5105 5740 275 Size: 382 635 1451 Fragments arranged by size: 1797 1451 936 654 637 635 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 5349 5747 6021 6168 6579 5349 Size: 398 274 147 411 5686 Fragments arranged by size: 5686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3737 3767 1178 Size: 30 165 30 2334 30 4327 Fragments arranged by size: 4327 2334 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 3135 3140 1186 Size: 1141 808 5 4962 Fragments arranged by size: 4962 1141 808 5 BseYI C'CCAG_C Cuts at: 2666 2706 6119 6615 2666 Size: 40 3413 496 2967 Fragments arranged by size: 3413 2967 496 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2281 2599 3343 1163 Size: 573 545 318 744 4736 Fragments arranged by size: 4736 744 573 545 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 2462 4366 5127 6155 1540 Size: 275 186 101 360 1904 761 1028 2301 Fragments arranged by size: 2301 1904 1028 761 360 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2649 3132 4029 4977 5062 6133 2649 Size: 483 897 948 85 1071 3432 Fragments arranged by size: 3432 1071 948 897 483 85 BsmI GAATG_Cn' Cuts at: 4427 4520 4427 Size: 93 6823 BsmBI CGTCTCn'nnnn_ Cuts at: 3150 4077 3150 Size: 927 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2649 2705 3132 3170 3781 4029 4235 Size: 399 859 56 427 38 611 248 206 Cuts at: 4235 4977 5062 6133 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 859 742 611 427 399 248 206 85 56 38 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 3927 4805 6535 3927 Size: 878 1730 4308 Fragments arranged by size: 4308 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2627 4271 844 Size: 1783 1644 3489 Fragments arranged by size: 3489 1783 1644 BsrBI CCG'CTC Cuts at: 1394 1823 4803 1394 Size: 429 2980 3507 Fragments arranged by size: 3507 2980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2557 2973 5411 5585 65 Size: 1183 879 430 416 2438 174 1396 Fragments arranged by size: 2438 1396 1183 879 430 416 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4123 5560 5747 1700 Size: 110 2313 1437 187 2869 Fragments arranged by size: 2869 2313 1437 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6916 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6916 BssSI C'ACGA_G Cuts at: 2642 4976 5988 2642 Size: 2334 1012 3570 Fragments arranged by size: 3570 2334 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 794 Size: 2021 4895 BstBI TT'CG_AA Cuts at: 5720 5720 Size: 6916 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6916 BstXI CCAn_nnnn'nTGG Cuts at: 2911 3211 3581 2911 Size: 300 370 6246 Fragments arranged by size: 6246 370 300 BstYI r'GATC_y Cuts at: 1972 3735 4246 4613 4998 5015 6467 6553 6565 Size: 1763 511 367 385 17 1452 86 12 Cuts at: 6565 6911 1972 Size: 346 1977 Fragments arranged by size: 1977 1763 1452 511 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3765 513 Size: 552 939 1761 3664 Fragments arranged by size: 3664 1761 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3951 487 Size: 683 56 2725 3452 Fragments arranged by size: 3452 2725 683 56 BtsI GCAGTG_nn' Cuts at: 2825 3637 4432 5226 5246 2825 Size: 812 795 794 20 4495 Fragments arranged by size: 4495 812 795 794 20 ClaI AT'CG_AT Cuts at: 1554 2391 3031 1554 Size: 837 640 5439 Fragments arranged by size: 5439 837 640 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6881 DraI TTT'AAA Cuts at: 4220 4220 Size: 6916 DraIII CAC_nnn'GTG Cuts at: 2661 2661 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2210 5923 809 Size: 507 894 3713 1802 Fragments arranged by size: 3713 1802 894 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 2158 2166 Size: 54 1425 50 275 18 269 59 8 Cuts at: 2166 2289 2459 2509 2523 2913 2953 3154 4363 Size: 123 170 50 14 390 40 201 1209 Cuts at: 4363 5252 6881 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1209 889 390 275 269 201 170 123 59 54 50 50 43 40 18 14 8 EagI C'GGCC_G Cuts at: 1537 1812 2099 2459 4363 1537 Size: 275 287 360 1904 4090 Fragments arranged by size: 4090 1904 360 287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2368 2427 3368 4113 4846 1353 Size: 1015 59 941 745 733 3423 Fragments arranged by size: 3423 1015 941 745 733 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5503 5877 6023 387 Size: 1064 138 3914 374 146 1280 Fragments arranged by size: 3914 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 2647 Size: 6916 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 3323 4052 4979 5576 6363 6631 Size: 393 749 942 729 927 597 787 268 Cuts at: 6631 1239 Size: 1524 Fragments arranged by size: 1524 942 927 787 749 729 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2093 2758 2784 3778 2093 Size: 665 26 994 5231 Fragments arranged by size: 5231 994 665 26 EcoRI G'AATT_C Cuts at: 4175 4225 4603 4175 Size: 50 378 6488 Fragments arranged by size: 6488 378 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4072 4072 Size: 6916 FspI TGC'GCA Cuts at: 5422 5422 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 3211 3505 3952 6063 1678 Size: 1533 294 447 2111 2531 Fragments arranged by size: 2531 2111 1533 447 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2223 2255 2377 2409 2556 Size: 32 135 32 651 32 122 32 147 Cuts at: 2556 2588 3370 3402 3539 3571 3922 3954 5557 Size: 32 782 32 137 32 351 32 1603 Cuts at: 5557 5589 5631 5663 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1603 782 651 351 147 137 135 122 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 2722 4260 4506 669 Size: 988 281 568 216 1538 246 3079 Fragments arranged by size: 3079 1538 988 568 281 246 216 HindIII A'AGCT_T Cuts at: 748 3921 748 Size: 3173 3743 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4149 4515 4149 Size: 366 6550 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3729 6030 6214 1305 Size: 2424 2301 184 2007 Fragments arranged by size: 2424 2301 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 2525 3156 10 Size: 54 1425 343 459 234 631 3770 Fragments arranged by size: 3770 1425 631 459 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2219 2621 3239 3579 4136 4874 Size: 648 843 210 402 618 340 557 738 Cuts at: 4874 5233 5392 518 Size: 359 159 2042 Fragments arranged by size: 2042 843 738 648 618 557 402 359 340 210 159 MspA1I CmG'CkG Cuts at: 1606 2710 2952 3160 4332 5009 6157 6402 1606 Size: 1104 242 208 1172 677 1148 245 2120 Fragments arranged by size: 2120 1172 1148 1104 677 245 242 208 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5425 Fragments arranged by size: 5425 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6916 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2458 2728 4274 5819 1132 Size: 97 1229 270 1546 1545 2229 Fragments arranged by size: 2229 1546 1545 1229 270 97 PasI CC'CwG_GG Cuts at: 1348 3781 1348 Size: 2433 4483 PciI A'CATG_T Cuts at: 5815 5815 Size: 6916 PflMI CCAn_nnn'nTGG Cuts at: 1108 2070 2089 1108 Size: 962 19 5935 Fragments arranged by size: 5935 962 19 PfoI T'CCnGG_A Cuts at: 4029 4029 Size: 6916 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2426 2458 4985 5017 6498 6530 2426 Size: 32 2527 32 1481 32 2812 Fragments arranged by size: 2812 2527 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2758 2784 2093 Size: 665 26 6225 Fragments arranged by size: 6225 665 26 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PspOMI G'GGCC_C Cuts at: 3777 3777 Size: 6916 PstI C_TGCA'G Cuts at: 830 2325 3547 3744 4268 830 Size: 1495 1222 197 524 3478 Fragments arranged by size: 3478 1495 1222 524 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 3160 3160 Size: 6916 SacI G_AGCT'C Cuts at: 2649 2649 Size: 6916 SalI G'TCGA_C Cuts at: 1936 4258 1936 Size: 2322 4594 SanDI GG'GwC_CC Cuts at: 2093 2784 2093 Size: 691 6225 SapI GCTCTTCn'nnn_ Cuts at: 2427 4113 2427 Size: 1686 5230 SbfI CC_TGCA'GG Cuts at: 3744 4268 3744 Size: 524 6392 ScaI AGT'ACT Cuts at: 2910 5164 6876 2910 Size: 2254 1712 2950 Fragments arranged by size: 2950 2254 1712 SexAI A'CCwGG_T Cuts at: 2694 2694 Size: 6916 SfcI C'TryA_G Cuts at: 826 1045 2321 3543 3592 3740 4264 5399 6080 Size: 219 1276 1222 49 148 524 1135 681 Cuts at: 6080 6271 826 Size: 191 1471 Fragments arranged by size: 1471 1276 1222 1135 681 524 219 191 148 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 4115 4243 1872 Size: 2243 128 4545 Fragments arranged by size: 4545 2243 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 3080 3284 5020 5921 6183 Size: 197 926 84 1053 204 1736 901 262 Cuts at: 6183 820 Size: 1553 Fragments arranged by size: 1736 1553 1053 926 901 262 204 197 84 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 2458 4274 1132 Size: 1326 1816 3774 Fragments arranged by size: 3774 1816 1326 SspI AAT'ATT Cuts at: 5 52 4840 5 Size: 47 4788 2081 Fragments arranged by size: 4788 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6916 StyI C'CwwG_G Cuts at: 513 1065 2004 3438 4355 513 Size: 552 939 1434 917 3074 Fragments arranged by size: 3074 1434 939 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3061 4012 4014 4943 4960 5113 5298 Size: 1255 1481 951 2 929 17 153 185 Cuts at: 5298 5743 325 Size: 445 1498 Fragments arranged by size: 1498 1481 1255 951 929 445 185 153 17 2 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2908 5162 6874 Size: 274 80 33 51 1443 931 2254 1712 Cuts at: 6874 96 Size: 138 Fragments arranged by size: 2254 1712 1443 931 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 6876 6908 2768 Size: 32 4076 32 2776 Fragments arranged by size: 4076 2776 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6916 XbaI T'CTAG_A Cuts at: 4252 4252 Size: 6916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 2881 Size: 6916 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6916 XmaI C'CCGG_G Cuts at: 1870 4113 4241 1870 Size: 2243 128 4545 Fragments arranged by size: 4545 2243 128 XmnI GAAnn'nnTTC Cuts at: 3675 3923 5045 3675 Size: 248 1122 5546 Fragments arranged by size: 5546 1122 248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3331 4087 4724 276 Size: 53 83 186 936 1797 756 637 2468 Fragments arranged by size: 2468 1797 936 756 637 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI