Restriction map for
pFN21AB9423
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AcsI AflII AflIII
AgeI AhdI AlwNI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HindIII
HinfI HpaI HphI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 496 GTCTAC GT^[AC][GT]AC
AccIII 320 TCCGGA T^CCGGA
AclI 415 AACGTT AA^CGTT
AcyI 108 GGCGTC G[GA]^CG[CT]C
Alw44I 490 GTGCAC G^TGCAC
Aor51HI 80 AGCGCT AGC^GCT
ApaI 279 GGGCCC GGGCC^C
ApaLI 490 GTGCAC G^TGCAC
AspHI 490 GTGCAC G[AT]GC[AT]^C
AvaI 156 CCCGGG C^[CT]CG[GA]G
BbsI 25 GTCTTC GAAGAC(2/6)
BpmI 613 CTGGAG CTGGAG(16/14)
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaHI 108 GGCGTC G[GA]^CG[CT]C
BsaWI 320 TCCGGA [AT]^CCGG[AT]
BseAI 320 TCCGGA T^CCGGA
BsiHKAI 490 GTGCAC G[AT]GC[AT]^C
BsoBI 156 CCCGGG C^[CT]CG[GA]G
BspEI 320 TCCGGA T^CCGGA
BstAPI 447 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 347 CCAGG CC^[AT]GG
BstYI 461 AGATCC [GA]^GATC[CT]
DraIII 190 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 329 CCACGG C^C[GA][CT]GG
EaeI 140 TGGCCG [CT]^GGCC[GA]
EarI 403 CTCTTC CTCTTC(1/4)
Eco47III 80 AGCGCT AGC^GCT
EcoRII 347 CCAGG ^CC[AT]GG
EcoT14I 605 CCAAGG C^C[AT][AT]GG
HaeII 80 AGCGCT [GA]GCGC^[CT]
Hin1I 108 GGCGTC G[GA]^CG[CT]C
HincII 214 GTCAAC GT[CT]^[GA]AC
HindII 214 GTCAAC GT[CT]^[GA]AC
Ksp632I 403 CTCTTC CTCTTC(1/4)
MflI 461 AGATCC [GA]^GATC[CT]
MroI 320 TCCGGA T^CCGGA
MseI 206 TTAA T^TAA
MvaI 347 CCAGG CC^[AT]GG
Psp1406I 415 AACGTT AA^CGTT
PstI 445 CTGCAG CTGCA^G
PvuII 148 CAGCTG CAG^CTG
SfaNI 518 GCATC GCATC(5/9)
SfcI 445 CTGCAG C^T[GA][CT]AG
SmaI 156 CCCGGG CCC^GGG
StyI 605 CCAAGG C^C[AT][AT]GG
Tru9I 206 TTAA T^TAA
XhoII 461 AGATCC [GA]^GATC[CT]
XmaCI 156 CCCGGG C^CCGGG
XmaI 156 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 294 GTAC GT^AC
351 GTAC
AlwI 462 GATCC GGATC(4/5)
374 GGATC
BanI 73 GGCACC G^G[CT][GA]CC
229 GGCACC
BanII 279 GGGCCC G[GA]GC[CT]^C
531 GGGCTC
BbrPI 190 CACGTG CAC^GTG
544 CACGTG
BsaAI 190 CACGTG [CT]AC^GT[GA]
544 CACGTG
BsrI 176 ACTGG ACTGG(1/-1)
371 ACTGG
BstXI 347 CCAGGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
605 CCAAGGAGCTGG
HgaI 109 GCGTC GACGC(5/10)
212 GCGTC
MspA1I 148 CAGCTG C[AC]G^C[GT]G
297 CCGCTG
NlaIII 270 CATG CATG^
354 CATG
PmaCI 190 CACGTG CAC^GTG
544 CACGTG
PmlI 190 CACGTG CAC^GTG
544 CACGTG
PpuMI 164 AGGACCT [GA]G^G[AT]CC[CT]
398 AGGACCT
RsaI 294 GTAC GT^AC
351 GTAC
TspRI 335 CACTG CA[GC]TG[ACGT][ACGT]^
620 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BspMI BssKI BstF5I
BstUI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MnlI MspI
MvnI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
TthHB8I