Restriction map for
pFN21AB9423
(Linear) (Six-base) MAPSORT of: KIBB9423.seq Check: 5199 from: 1 to: 651
KIBB9423.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 562 651
Size: 562 89
AccI GT'mk_AC
Cuts at: 0 497 651
Size: 497 154
AclI AA'CG_TT
Cuts at: 0 416 651
Size: 416 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 651
Size: 37 614
AfeI AGC'GCT
Cuts at: 0 82 651
Size: 82 569
ApaI G_GGCC'C
Cuts at: 0 283 651
Size: 283 368
ApaLI G'TGCA_C
Cuts at: 0 490 651
Size: 490 161
AvaI C'yCGr_G
Cuts at: 0 156 651
Size: 156 495
BanI G'GyrC_C
Cuts at: 0 73 229 651
Size: 73 156 422
Fragments arranged by size:
422 156 73
BanII G_rGCy'C
Cuts at: 0 283 535 651
Size: 283 252 116
Fragments arranged by size:
283 252 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 651
Size: 18 633
Bme1580I G_kGCm'C
Cuts at: 0 76 283 494 651
Size: 76 207 211 157
Fragments arranged by size:
211 207 157 76
BmgBI CAC'GTC
Cuts at: 0 495 651
Size: 495 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 651
Size: 186 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 651
Size: 634 17
BsaAI yAC'GTr
Cuts at: 0 192 546 651
Size: 192 354 105
Fragments arranged by size:
354 192 105
BsaHI Gr'CG_yC
Cuts at: 0 109 651
Size: 109 542
BsaWI w'CCGG_w
Cuts at: 0 320 651
Size: 320 331
BseYI C'CCAG_C
Cuts at: 0 77 651
Size: 77 574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 166 231 651
Size: 76 90 65 420
Fragments arranged by size:
420 90 76 65
BsiHKAI G_wGCw'C
Cuts at: 0 494 651
Size: 494 157
Bsp1286I G_dGCh'C
Cuts at: 0 76 283 494 535 651
Size: 76 207 211 41 116
Fragments arranged by size:
211 207 116 76 41
BspEI T'CCGG_A
Cuts at: 0 320 651
Size: 320 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 368 562 651
Size: 140 228 194 89
Fragments arranged by size:
228 194 140 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 453 651
Size: 453 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 612 651
Size: 354 258 39
Fragments arranged by size:
354 258 39
BstYI r'GATC_y
Cuts at: 0 461 651
Size: 461 190
BtgI C'CryG_G
Cuts at: 0 329 651
Size: 329 322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 504 651
Size: 504 147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 651
Size: 399 35 217
Fragments arranged by size:
399 217 35
DraIII CAC_nnn'GTG
Cuts at: 0 195 651
Size: 195 456
EaeI y'GGCC_r
Cuts at: 0 140 651
Size: 140 511
EarI CTCTTCn'nnn_
Cuts at: 0 409 651
Size: 409 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 651
Size: 37 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 634 651
Size: 37 597 17
Fragments arranged by size:
597 37 17
EcoO109I rG'GnC_Cy
Cuts at: 0 165 279 280 399 651
Size: 165 114 1 119 252
Fragments arranged by size:
252 165 119 114 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 651
Size: 42 609
HaeII r_GCGC'y
Cuts at: 0 84 651
Size: 84 567
HincII GTy'rAC
Cuts at: 0 216 651
Size: 216 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 651
Size: 29 622
MspA1I CmG'CkG
Cuts at: 0 150 299 651
Size: 150 149 352
Fragments arranged by size:
352 150 149
PmlI CAC'GTG
Cuts at: 0 192 546 651
Size: 192 354 105
Fragments arranged by size:
354 192 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 651
Size: 301 32 318
Fragments arranged by size:
318 301 32
PpuMI rG'GwC_Cy
Cuts at: 0 165 399 651
Size: 165 234 252
Fragments arranged by size:
252 234 165
PspOMI G'GGCC_C
Cuts at: 0 279 651
Size: 279 372
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 541 573 651
Size: 541 32 78
Fragments arranged by size:
541 78 32
PstI C_TGCA'G
Cuts at: 0 449 651
Size: 449 202
PvuII CAG'CTG
Cuts at: 0 150 651
Size: 150 501
SfcI C'TryA_G
Cuts at: 0 445 651
Size: 445 206
SmaI CCC'GGG
Cuts at: 0 158 651
Size: 158 493
StyI C'CwwG_G
Cuts at: 0 605 651
Size: 605 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 168 651
Size: 91 77 483
Fragments arranged by size:
483 91 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 414 446 651
Size: 414 32 205
Fragments arranged by size:
414 205 32
XmaI C'CCGG_G
Cuts at: 0 156 651
Size: 156 495
Enzymes that do cut:
AarI AccI AclI AcuI AfeI ApaI ApaLI AvaI
BanI BanII BbsI Bme1580I BmgBI BmrI BpmI BsaAI
BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BstAPI BstXI BstYI BtgI BtgZI CspCI DraIII EaeI
EarI EciI Eco57MI EcoO109I FalI HaeII HincII MmeI
MspA1I PmlI PpiI PpuMI PspOMI PsrI PstI PvuII
SfcI SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI DraI DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI