(Circular) (Six-base) MAPSORT of: pFN21AB9422.seq Check: 2950 from: 1 to: 6418 pFN21AB9422 6418 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3761 1937 Size: 1824 4594 Acc65I G'GTAC_C Cuts at: 3739 3739 Size: 6418 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 3275 4481 5865 6133 1632 Size: 945 698 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 945 698 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6221 AflIII A'CryG_T Cuts at: 1203 2103 3684 5317 6294 1203 Size: 900 1581 1633 977 1327 Fragments arranged by size: 1633 1581 1327 977 900 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3354 1184 Size: 2170 4248 AlwNI CAG_nnn'CTG Cuts at: 2695 3615 4161 5733 2695 Size: 920 546 1572 3380 Fragments arranged by size: 3380 1572 920 546 ApaI G_GGCC'C Cuts at: 2607 2607 Size: 6418 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 3007 3438 3727 3953 4105 6214 1493 Size: 1514 431 289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 431 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 3743 1870 Size: 73 1800 4545 Fragments arranged by size: 4545 1800 73 BamHI G'GATC_C Cuts at: 3748 4115 3748 Size: 367 6051 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3739 5194 618 Size: 325 217 627 1952 1455 1842 Fragments arranged by size: 1952 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2145 2607 3385 3737 1391 Size: 754 462 778 352 4072 Fragments arranged by size: 4072 778 754 462 352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3513 928 Size: 971 1614 3833 Fragments arranged by size: 3833 1614 971 BbvCI CC'TCA_GC Cuts at: 3603 3603 Size: 6418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6418 BfrBI ATG'CAT Cuts at: 3194 3194 Size: 6418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5029 136 Size: 107 122 71 631 605 3357 1525 Fragments arranged by size: 3357 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2636 6413 2636 Size: 3777 2641 BlpI GC'TnA_GC Cuts at: 2955 3835 2955 Size: 880 5538 Bme1580I G_kGCm'C Cuts at: 1790 2607 4479 5635 1790 Size: 817 1872 1156 2573 Fragments arranged by size: 2573 1872 1156 817 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 4149 5107 Size: 416 53 83 399 24 629 2103 958 Cuts at: 5107 442 Size: 1753 Fragments arranged by size: 2103 1753 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2374 2497 2882 5078 1239 Size: 1135 123 385 2196 2579 Fragments arranged by size: 2579 2196 1135 385 123 Bpu10I CC'TnA_GC Cuts at: 2432 3603 2432 Size: 1171 5247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 2486 2732 4543 5408 5706 2323 Size: 163 246 1811 865 298 3035 Fragments arranged by size: 3035 1811 865 298 246 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5081 882 Size: 617 3582 2219 Fragments arranged by size: 3582 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3700 4209 493 Size: 711 2496 509 2702 Fragments arranged by size: 2702 2496 711 509 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4225 4607 5242 275 Size: 53 83 186 936 2692 382 635 1451 Fragments arranged by size: 2692 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4851 5249 5523 5670 6081 4851 Size: 398 274 147 411 5188 Fragments arranged by size: 5188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2880 2910 1178 Size: 30 165 30 1477 30 4686 Fragments arranged by size: 4686 1477 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2228 2512 2687 3612 1186 Size: 946 96 284 175 925 3992 Fragments arranged by size: 3992 946 925 284 175 96 BseYI C'CCAG_C Cuts at: 5621 6117 5621 Size: 496 5922 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5845 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2831 4479 4564 5635 2145 Size: 686 1648 85 1071 2928 Fragments arranged by size: 2928 1648 1071 686 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2607 2831 3385 3737 4479 4564 Size: 399 355 462 224 554 352 742 85 Cuts at: 4564 5635 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 554 462 399 355 352 224 85 BspEI T'CCGG_A Cuts at: 6081 6081 Size: 6418 BspHI T'CATG_A Cuts at: 2308 3442 4307 6037 2308 Size: 1134 865 1730 2689 Fragments arranged by size: 2689 1730 1134 865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3773 844 Size: 2929 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3336 3464 4913 5087 65 Size: 1183 2088 128 1449 174 1396 Fragments arranged by size: 2088 1449 1396 1183 174 128 BsrFI r'CCGG_y Cuts at: 1700 1810 5062 5249 1700 Size: 110 3252 187 2869 Fragments arranged by size: 3252 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 2608 2629 4478 5490 2608 Size: 21 1849 1012 3536 Fragments arranged by size: 3536 1849 1012 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6418 BstBI TT'CG_AA Cuts at: 2664 3504 5222 2664 Size: 840 1718 3860 Fragments arranged by size: 3860 1718 840 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2636 3748 4115 4500 4517 5969 6055 6067 Size: 664 1112 367 385 17 1452 86 12 Cuts at: 6067 6413 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1112 664 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2705 3377 2705 Size: 672 5746 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5866 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5679 Fragments arranged by size: 5679 683 56 BtsI GCAGTG_nn' Cuts at: 3255 3934 4728 4748 3255 Size: 679 794 20 4925 Fragments arranged by size: 4925 794 679 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3865 4754 6383 Size: 54 1425 50 275 18 2035 889 1629 Cuts at: 6383 8 Size: 43 Fragments arranged by size: 2035 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2844 2963 3345 4348 1353 Size: 972 519 119 382 1003 3423 Fragments arranged by size: 3423 1003 972 519 382 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2143 2143 Size: 6418 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2374 2497 2577 2882 3275 4481 5078 Size: 393 742 123 80 305 393 1206 597 Cuts at: 5078 5865 6133 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1206 787 742 597 393 393 305 268 123 80 EcoO109I rG'GnC_Cy Cuts at: 2374 2436 2992 3355 2374 Size: 62 556 363 5437 Fragments arranged by size: 5437 556 363 62 EcoRI G'AATT_C Cuts at: 3727 4105 3727 Size: 378 6040 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 6418 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 5565 1678 Size: 3887 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3119 3151 3526 3558 5059 Size: 32 135 32 1547 32 375 32 1501 Cuts at: 5059 5091 5133 5165 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1547 1501 375 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 3762 4008 669 Size: 988 281 741 1083 246 3079 Fragments arranged by size: 3079 1083 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 3170 748 Size: 2422 3996 HpaI GTT'AAC Cuts at: 2679 4008 2679 Size: 1329 5089 KpnI G_GTAC'C Cuts at: 3743 3743 Size: 6418 MfeI C'AATT_G Cuts at: 3204 4017 3204 Size: 813 5605 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2851 5532 5716 1305 Size: 1546 2681 184 2007 Fragments arranged by size: 2681 2007 1546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4596 Fragments arranged by size: 4596 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3189 4376 4735 4894 518 Size: 648 1147 876 1187 359 159 2042 Fragments arranged by size: 2042 1187 1147 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2164 2517 3310 3472 3628 3834 4511 Size: 419 139 353 793 162 156 206 677 Cuts at: 4511 5659 5904 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 793 677 419 353 245 206 162 156 139 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5866 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NsiI A_TGCA'T Cuts at: 3196 3196 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 3091 3776 5321 1132 Size: 97 1862 685 1545 2229 Fragments arranged by size: 2229 1862 1545 685 97 PasI CC'CwG_GG Cuts at: 1348 2400 1348 Size: 1052 5366 PciI A'CATG_T Cuts at: 5317 5317 Size: 6418 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6418 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PmlI CAC'GTG Cuts at: 3700 3700 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2484 2516 4487 4519 6000 6032 2484 Size: 32 1971 32 1481 32 2870 Fragments arranged by size: 2870 1971 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2436 3355 2374 Size: 62 919 5437 Fragments arranged by size: 5437 919 62 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6418 PsiI TTA'TAA Cuts at: 3988 3988 Size: 6418 PspOMI G'GGCC_C Cuts at: 2603 2603 Size: 6418 PstI C_TGCA'G Cuts at: 830 2130 2499 2644 3770 830 Size: 1300 369 145 1126 3478 Fragments arranged by size: 3478 1300 1126 369 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 PvuII CAG'CTG Cuts at: 2025 2164 3310 3472 2025 Size: 139 1146 162 4971 Fragments arranged by size: 4971 1146 162 139 SacI G_AGCT'C Cuts at: 2145 2145 Size: 6418 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SapI GCTCTTCn'nnn_ Cuts at: 2325 2325 Size: 6418 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SfcI C'TryA_G Cuts at: 826 1045 2126 2495 2640 3152 3766 4901 5582 Size: 219 1081 369 145 512 614 1135 681 Cuts at: 5582 5773 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1081 681 614 512 369 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2302 2501 2747 4522 5423 5685 Size: 197 926 359 199 246 1775 901 262 Cuts at: 5685 820 Size: 1553 Fragments arranged by size: 1775 1553 926 901 359 262 246 199 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3091 3776 1132 Size: 1959 685 3774 Fragments arranged by size: 3774 1959 685 SspI AAT'ATT Cuts at: 5 52 3634 4342 5 Size: 47 3582 708 2081 Fragments arranged by size: 3582 2081 708 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6418 StyI C'CwwG_G Cuts at: 513 1065 3857 513 Size: 552 2792 3074 Fragments arranged by size: 3074 2792 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 2165 4445 4462 4615 4800 5245 Size: 1255 526 59 2280 17 153 185 445 Cuts at: 5245 325 Size: 1498 Fragments arranged by size: 2280 1498 1255 526 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4664 6376 96 Size: 274 80 33 51 1443 2687 1712 138 Fragments arranged by size: 2687 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2865 2880 2897 2912 6378 6410 2865 Size: 15 17 15 3466 32 2873 Fragments arranged by size: 3466 2873 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 2660 4547 2660 Size: 1887 4531 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI