Restriction map for
pFN21AB9422
(Linear) (Six-base) MAPSORT of: KIBB9422.seq Check: 8350 from: 1 to: 1713
KIBB9422.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1270 1713
Size: 572 698 443
Fragments arranged by size:
698 572 443
AflIII A'CryG_T
Cuts at: 0 98 1679 1713
Size: 98 1581 34
Fragments arranged by size:
1581 98 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1349 1713
Size: 1349 364
AlwNI CAG_nnn'CTG
Cuts at: 0 690 1610 1713
Size: 690 920 103
Fragments arranged by size:
920 690 103
ApaI G_GGCC'C
Cuts at: 0 602 1713
Size: 602 1111
ApoI r'AATT_y
Cuts at: 0 1002 1433 1713
Size: 1002 431 280
Fragments arranged by size:
1002 431 280
BanII G_rGCy'C
Cuts at: 0 140 602 1380 1713
Size: 140 462 778 333
Fragments arranged by size:
778 462 333 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1508 1713
Size: 1508 205
BbvCI CC'TCA_GC
Cuts at: 0 1598 1713
Size: 1598 115
BfrBI ATG'CAT
Cuts at: 0 1189 1713
Size: 1189 524
BglII A'GATC_T
Cuts at: 0 631 1713
Size: 631 1082
BlpI GC'TnA_GC
Cuts at: 0 950 1713
Size: 950 763
Bme1580I G_kGCm'C
Cuts at: 0 602 1713
Size: 602 1111
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 1713
Size: 41 1672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 492 877 1713
Size: 369 123 385 836
Fragments arranged by size:
836 385 369 123
Bpu10I CC'TnA_GC
Cuts at: 0 427 1598 1713
Size: 427 1171 115
Fragments arranged by size:
1171 427 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 481 727 1713
Size: 318 163 246 986
Fragments arranged by size:
986 318 246 163
BsaAI yAC'GTr
Cuts at: 0 1695 1713
Size: 1695 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 875 905 1713
Size: 875 30 808
Fragments arranged by size:
875 808 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 223 507 682 1607 1713
Size: 127 96 284 175 925 106
Fragments arranged by size:
925 284 175 127 106 96
BsiHKAI G_wGCw'C
Cuts at: 0 140 826 1713
Size: 140 686 887
Fragments arranged by size:
887 686 140
Bsp1286I G_dGCh'C
Cuts at: 0 140 602 826 1380 1713
Size: 140 462 224 554 333
Fragments arranged by size:
554 462 333 224 140
BspHI T'CATG_A
Cuts at: 0 303 1437 1713
Size: 303 1134 276
Fragments arranged by size:
1134 303 276
BsrDI GCAATG_nn'
Cuts at: 0 1331 1459 1713
Size: 1331 128 254
Fragments arranged by size:
1331 254 128
BssSI C'ACGA_G
Cuts at: 0 603 624 1713
Size: 603 21 1089
Fragments arranged by size:
1089 603 21
BstBI TT'CG_AA
Cuts at: 0 659 1499 1713
Size: 659 840 214
Fragments arranged by size:
840 659 214
BstYI r'GATC_y
Cuts at: 0 631 1713
Size: 631 1082
Bsu36I CC'TnA_GG
Cuts at: 0 700 1372 1713
Size: 700 672 341
Fragments arranged by size:
700 672 341
BtsI GCAGTG_nn'
Cuts at: 0 1250 1713
Size: 1250 463
EarI CTCTTCn'nnn_
Cuts at: 0 320 839 958 1340 1713
Size: 320 519 119 382 373
Fragments arranged by size:
519 382 373 320 119
EcoICRI GAG'CTC
Cuts at: 0 138 1713
Size: 138 1575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 492 572 877 1270 1713
Size: 369 123 80 305 393 443
Fragments arranged by size:
443 393 369 305 123 80
EcoO109I rG'GnC_Cy
Cuts at: 0 369 431 987 1350 1713
Size: 369 62 556 363 363
Fragments arranged by size:
556 369 363 363 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 672 1713
Size: 672 1041
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 1521 1553 1713
Size: 1114 32 375 32 160
Fragments arranged by size:
1114 375 160 32 32
HincII GTy'rAC
Cuts at: 0 674 1713
Size: 674 1039
HindIII A'AGCT_T
Cuts at: 0 1165 1713
Size: 1165 548
HpaI GTT'AAC
Cuts at: 0 674 1713
Size: 674 1039
MfeI C'AATT_G
Cuts at: 0 1199 1713
Size: 1199 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1713
Size: 846 867
MslI CAynn'nnrTG
Cuts at: 0 308 1184 1713
Size: 308 876 529
Fragments arranged by size:
876 529 308
MspA1I CmG'CkG
Cuts at: 0 20 159 512 1305 1467 1623 1713
Size: 20 139 353 793 162 156 90
Fragments arranged by size:
793 353 162 156 139 90 20
NsiI A_TGCA'T
Cuts at: 0 1191 1713
Size: 1191 522
NspI r_CATG'y
Cuts at: 0 1086 1713
Size: 1086 627
PasI CC'CwG_GG
Cuts at: 0 395 1713
Size: 395 1318
PmlI CAC'GTG
Cuts at: 0 1695 1713
Size: 1695 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 479 511 1713
Size: 479 32 1202
Fragments arranged by size:
1202 479 32
PpuMI rG'GwC_Cy
Cuts at: 0 369 431 1350 1713
Size: 369 62 919 363
Fragments arranged by size:
919 369 363 62
PspOMI G'GGCC_C
Cuts at: 0 598 1713
Size: 598 1115
PstI C_TGCA'G
Cuts at: 0 125 494 639 1713
Size: 125 369 145 1074
Fragments arranged by size:
1074 369 145 125
PvuII CAG'CTG
Cuts at: 0 20 159 1305 1467 1713
Size: 20 139 1146 162 246
Fragments arranged by size:
1146 246 162 139 20
SacI G_AGCT'C
Cuts at: 0 140 1713
Size: 140 1573
SapI GCTCTTCn'nnn_
Cuts at: 0 320 1713
Size: 320 1393
SfcI C'TryA_G
Cuts at: 0 121 490 635 1147 1713
Size: 121 369 145 512 566
Fragments arranged by size:
566 512 369 145 121
SmlI C'TyrA_G
Cuts at: 0 297 496 742 1713
Size: 297 199 246 971
Fragments arranged by size:
971 297 246 199
SphI G_CATG'C
Cuts at: 0 1086 1713
Size: 1086 627
SspI AAT'ATT
Cuts at: 0 1629 1713
Size: 1629 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 160 1713
Size: 101 59 1553
Fragments arranged by size:
1553 101 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 860 875 892 907 1713
Size: 860 15 17 15 806
Fragments arranged by size:
860 806 17 15 15
XmnI GAAnn'nnTTC
Cuts at: 0 655 1713
Size: 655 1058
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI BanII BbsI
BbvCI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaXI BseRI BsiHKAI Bsp1286I BspHI BsrDI
BssSI BstBI BstYI Bsu36I BtsI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I HincII HindIII HpaI MfeI MmeI
MslI MspA1I NsiI NspI PasI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SapI SfcI SmlI SphI
SspI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BglI BmgBI BmtI BplI BsaI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PflMI
PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI StyI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI