Restriction map for
pFN21AB9414
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 131 GAATTC [GA]^AATT[CT]
AflII 556 CTTAAG C^TTAAG
AflIII 309 ACGTGT A^C[GA][CT]GT
Aor51HI 1942 AGCGCT AGC^GCT
ApoI 131 GAATTC [GA]^AATT[CT]
BamHI 1613 GGATCC G^GATCC
BanII 55 GAGCCC G[GA]GC[CT]^C
BcgI 126 GCATCGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 556 CTTAAG C^TTAAG
BsaAI 308 TACGTG [CT]AC^GT[GA]
BsaBI 1882 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 491 TCCGGT [AT]^CCGG[AT]
BsgI 1996 CTGCAC GTGCAG(16/14)
BsmAI 3 GTCTC GTCTC(1/5)
BspHI 639 TCATGA T^CATGA
BsrBI 1448 GAGCGG CCGCTC(-3/-3)
DraII 1916 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 659 CGGCCA [CT]^GGCC[GA]
Eco47III 1942 AGCGCT AGC^GCT
EcoNI 973 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1916 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 131 GAATTC G^AATTC
HapII 492 CCGG C^CGG
HincII 526 GTCAAC GT[CT]^[GA]AC
HindII 526 GTCAAC GT[CT]^[GA]AC
HpaII 492 CCGG C^CGG
MamI 1882 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1923 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 492 CCGG C^CGG
NdeI 443 CATATG CA^TATG
PvuII 1812 CAGCTG CAG^CTG
RcaI 639 TCATGA T^CATGA
SapI 217 GAAGAGC GCTCTTC(1/4)
SexAI 909 ACCTGGT A^CC[AT]GGT
SmlI 556 CTTAAG C^T[CT][GA]AG
SspI 389 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 962 GTGCAC G^TGCAC
1757 GTGCAC
AlwNI 1083 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1346 CAGGCTCTG
ApaLI 962 GTGCAC G^TGCAC
1757 GTGCAC
AspHI 962 GTGCAC G[AT]GC[AT]^C
1757 GTGCAC
BseRI 1314 CTCCTC GAGGAG(10/8)
2024 CTCCTC
BsiHKAI 962 GTGCAC G[AT]GC[AT]^C
1757 GTGCAC
BsmFI 1799 GGGAC GGGAC(10/14)
969 GTCCC
BstYI 1613 GGATCC [GA]^GATC[CT]
1988 AGATCC
DraI 375 TTTAAA TTT^AAA
1650 TTTAAA
EcoRV 73 GATATC GAT^ATC
879 GATATC
EcoT14I 59 CCTTGG C^C[AT][AT]GG
1495 CCTTGG
HgaI 502 GACGC GACGC(5/10)
1849 GCGTC
HphI 862 GGTGA GGTGA(8/7)
901 TCACC
MaeII 309 ACGT A^CGT
1765 ACGT
MflI 1613 GGATCC [GA]^GATC[CT]
1988 AGATCC
MspA1I 1812 CAGCTG C[AC]G^C[GT]G
1993 CCGCTG
NspI 163 GCATGT [GA]CATG^[CT]
629 GCATGT
PleI 1870 GACTC GAGTC(4/5)
994 GAGTC
StyI 59 CCTTGG C^C[AT][AT]GG
1495 CCTTGG
XhoII 1613 GGATCC [GA]^GATC[CT]
1988 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I