(Circular) (Six-base) MAPSORT of: pFN21AB9414.seq Check: 6195 from: 1 to: 6844 pFN21AB9414 6844 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4654 278 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4187 1937 Size: 2250 4594 Acc65I G'GTAC_C Cuts at: 4165 4165 Size: 6844 AclI AA'CG_TT Cuts at: 1563 4971 5344 1563 Size: 3408 373 3063 Fragments arranged by size: 3408 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2744 2826 3296 3843 3902 3967 4069 4907 Size: 1112 82 470 547 59 65 102 838 Cuts at: 4907 6291 6559 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1112 838 547 470 268 102 82 65 59 AfeI AGC'GCT Cuts at: 3949 3949 Size: 6844 AflII C'TTAA_G Cuts at: 820 1017 2561 820 Size: 197 1544 5103 Fragments arranged by size: 5103 1544 197 AflIII A'CryG_T Cuts at: 1203 2314 5743 6720 1203 Size: 1111 3429 977 1327 Fragments arranged by size: 3429 1327 1111 977 AgeI A'CCGG_T Cuts at: 5675 5675 Size: 6844 AhdI GACnn_n'nnGTC Cuts at: 5573 5573 Size: 6844 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6844 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3390 1184 Size: 2206 4638 AlwNI CAG_nnn'CTG Cuts at: 3093 3356 4587 6159 3093 Size: 263 1231 1572 3778 Fragments arranged by size: 3778 1572 1231 263 ApaLI G'TGCA_C Cuts at: 2967 3762 4901 6057 2967 Size: 795 1139 1156 3754 Fragments arranged by size: 3754 1156 1139 795 ApoI r'AATT_y Cuts at: 1493 2136 4153 4379 4531 6640 1493 Size: 643 2017 226 152 2109 1697 Fragments arranged by size: 2109 2017 1697 643 226 152 AseI AT'TA_AT Cuts at: 160 1032 5398 160 Size: 872 4366 1606 Fragments arranged by size: 4366 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 AvaI C'yCGr_G Cuts at: 1870 1943 4169 1870 Size: 73 2226 4545 Fragments arranged by size: 4545 2226 73 BamHI G'GATC_C Cuts at: 3618 4174 4541 3618 Size: 556 367 5921 Fragments arranged by size: 5921 556 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4165 5620 618 Size: 325 217 627 2378 1455 1842 Fragments arranged by size: 2378 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 4163 1391 Size: 673 2099 4072 Fragments arranged by size: 4072 2099 673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2196 2511 3849 928 Size: 971 138 159 315 1338 3923 Fragments arranged by size: 3923 1338 971 315 159 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 5035 5069 1903 Size: 34 183 34 2881 34 3678 Fragments arranged by size: 3678 2881 183 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4736 5946 4736 Size: 1210 5634 BclI T'GATC_A Cuts at: 1548 1548 Size: 6844 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5455 136 Size: 107 122 71 631 605 3783 1525 Fragments arranged by size: 3783 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6839 6839 Size: 6844 BlpI GC'TnA_GC Cuts at: 4261 4261 Size: 6844 Bme1580I G_kGCm'C Cuts at: 1790 2048 2971 3691 3766 4905 6061 1790 Size: 258 923 720 75 1139 1156 2573 Fragments arranged by size: 2573 1156 1139 923 720 258 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6844 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3137 4575 5533 Size: 416 53 83 399 24 1720 1438 958 Cuts at: 5533 442 Size: 1753 Fragments arranged by size: 1753 1720 1438 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6844 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3450 3450 Size: 6844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5504 1239 Size: 4265 2579 Bpu10I CC'TnA_GC Cuts at: 3247 3683 3247 Size: 436 6408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4969 5834 6132 4969 Size: 865 298 5681 Fragments arranged by size: 5681 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5507 882 Size: 617 4008 2219 Fragments arranged by size: 4008 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2315 4635 493 Size: 711 1111 2320 2702 Fragments arranged by size: 2702 2320 1111 711 BsaBI GATnn'nnATC Cuts at: 3891 6838 3891 Size: 2947 3897 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4651 5033 5668 275 Size: 53 83 186 936 3118 382 635 1451 Fragments arranged by size: 3118 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2496 5277 5675 5949 6096 6507 2496 Size: 2781 398 274 147 411 2833 Fragments arranged by size: 2833 2781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6619 Fragments arranged by size: 6619 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3310 4020 1186 Size: 2124 710 4010 Fragments arranged by size: 4010 2124 710 BseYI C'CCAG_C Cuts at: 2194 2877 3068 3829 3944 4094 6047 6543 2194 Size: 683 191 761 115 150 1953 496 2495 Fragments arranged by size: 2495 1953 761 683 496 191 150 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3986 1163 Size: 573 2250 4021 Fragments arranged by size: 4021 2250 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4294 5055 6083 1540 Size: 275 186 2293 761 1028 2301 Fragments arranged by size: 2301 2293 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2971 3766 4905 4990 6061 2971 Size: 795 1139 85 1071 3754 Fragments arranged by size: 3754 1139 1071 795 85 BsmI GAATG_Cn' Cuts at: 4355 4448 4355 Size: 93 6751 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2064 2971 3691 3766 4163 4905 Size: 399 258 16 907 720 75 397 742 Cuts at: 4905 4990 6061 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 720 399 397 258 85 75 16 BspEI T'CCGG_A Cuts at: 6507 6507 Size: 6844 BspHI T'CATG_A Cuts at: 2644 4733 6463 2644 Size: 2089 1730 3025 Fragments arranged by size: 3025 2089 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4199 844 Size: 3355 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3455 4731 1394 Size: 429 1632 1276 3507 Fragments arranged by size: 3507 1632 1276 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5339 5513 65 Size: 1183 4091 174 1396 Fragments arranged by size: 4091 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5488 5675 1700 Size: 110 3678 187 2869 Fragments arranged by size: 3678 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6844 BssSI C'ACGA_G Cuts at: 4904 5916 4904 Size: 1012 5832 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6844 BstBI TT'CG_AA Cuts at: 5648 5648 Size: 6844 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6844 BstYI r'GATC_y Cuts at: 1972 3618 3993 4174 4541 4926 4943 6395 6481 Size: 1646 375 181 367 385 17 1452 86 Cuts at: 6481 6493 6839 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1646 1452 385 375 367 346 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6105 Fragments arranged by size: 6105 683 56 BtsI GCAGTG_nn' Cuts at: 2621 2807 3777 4360 5154 5174 2621 Size: 186 970 583 794 20 4291 Fragments arranged by size: 4291 970 794 583 186 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6844 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2901 2936 3700 3735 529 Size: 35 2337 35 764 35 3638 Fragments arranged by size: 3638 2337 764 35 35 35 DraI TTT'AAA Cuts at: 2382 3657 4148 2382 Size: 1275 491 5078 Fragments arranged by size: 5078 1275 491 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5851 809 Size: 507 4535 1802 Fragments arranged by size: 4535 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2664 4291 5180 Size: 54 1425 50 275 18 834 1627 889 Cuts at: 5180 6809 8 Size: 1629 43 Fragments arranged by size: 1629 1627 1425 889 834 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4291 1537 Size: 275 2479 4090 Fragments arranged by size: 4090 2479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2217 3128 3315 3328 3885 4774 1353 Size: 864 911 187 13 557 889 3423 Fragments arranged by size: 3423 911 889 864 557 187 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5431 5805 5951 387 Size: 1064 138 3842 374 146 1280 Fragments arranged by size: 3842 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2744 2826 3296 3843 3902 3967 4069 Size: 393 1112 82 470 547 59 65 102 Cuts at: 4069 4907 5504 6291 6559 1239 Size: 838 597 787 268 1524 Fragments arranged by size: 1524 1112 838 787 597 547 470 393 268 102 82 65 59 EcoNI CCTnn'n_nnAGG Cuts at: 2982 2982 Size: 6844 EcoO109I rG'GnC_Cy Cuts at: 3922 3922 Size: 6844 EcoRI G'AATT_C Cuts at: 2136 4153 4531 2136 Size: 2017 378 4449 Fragments arranged by size: 4449 2017 378 EcoRV GAT'ATC Cuts at: 2080 2886 2080 Size: 806 6038 FspI TGC'GCA Cuts at: 5350 5350 Size: 6844 HaeII r_GCGC'y Cuts at: 1678 2362 3951 3978 5991 1678 Size: 684 1589 27 2013 2531 Fragments arranged by size: 2531 2013 1589 684 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2990 3022 5485 5517 5559 Size: 32 135 32 1418 32 2463 32 42 Cuts at: 5559 5591 1373 Size: 32 2626 Fragments arranged by size: 2626 2463 1418 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 4188 4434 669 Size: 988 281 595 1655 246 3079 Fragments arranged by size: 3079 1655 988 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6844 HpaI GTT'AAC Cuts at: 4434 4434 Size: 6844 KpnI G_GTAC'C Cuts at: 4169 4169 Size: 6844 MfeI C'AATT_G Cuts at: 4443 4443 Size: 6844 MluI A'CGCG_T Cuts at: 6720 6720 Size: 6844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3771 3798 3995 5958 6142 1305 Size: 2466 27 197 1963 184 2007 Fragments arranged by size: 2466 2007 1963 197 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5022 Fragments arranged by size: 5022 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4802 5161 5320 518 Size: 648 2766 870 359 159 2042 Fragments arranged by size: 2766 2042 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3819 4000 4260 4937 6085 6330 1606 Size: 2213 181 260 677 1148 245 2120 Fragments arranged by size: 2213 2120 1148 677 260 245 181 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6292 NdeI CA'TA_TG Cuts at: 387 2449 387 Size: 2062 4782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6844 NotI GC'GGCC_GC Cuts at: 4291 4291 Size: 6844 NspI r_CATG'y Cuts at: 1132 1229 2172 2638 4202 5747 1132 Size: 97 943 466 1564 1545 2229 Fragments arranged by size: 2229 1564 1545 943 466 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6844 PciI A'CATG_T Cuts at: 5743 5743 Size: 6844 PflMI CCAn_nnn'nTGG Cuts at: 1108 2915 3093 3356 1108 Size: 1807 178 263 4596 Fragments arranged by size: 4596 1807 263 178 PmeI GTTT'AAAC Cuts at: 4148 4148 Size: 6844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4913 4945 6426 6458 4913 Size: 32 1481 32 5299 Fragments arranged by size: 5299 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6844 PsiI TTA'TAA Cuts at: 2205 3462 4414 2205 Size: 1257 952 4635 Fragments arranged by size: 4635 1257 952 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2706 2738 2706 Size: 32 6812 PstI C_TGCA'G Cuts at: 830 2594 2814 3379 4196 830 Size: 1764 220 565 817 3478 Fragments arranged by size: 3478 1764 817 565 220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6844 PvuII CAG'CTG Cuts at: 3819 3819 Size: 6844 SalI G'TCGA_C Cuts at: 1936 4186 1936 Size: 2250 4594 SapI GCTCTTCn'nnn_ Cuts at: 2217 2217 Size: 6844 SbfI CC_TGCA'GG Cuts at: 4196 4196 Size: 6844 ScaI AGT'ACT Cuts at: 5092 6804 5092 Size: 1712 5132 SexAI A'CCwGG_T Cuts at: 2914 2914 Size: 6844 SfcI C'TryA_G Cuts at: 826 1045 2590 2810 3099 3156 3375 3624 3645 Size: 219 1545 220 289 57 219 249 21 Cuts at: 3645 4118 4192 5327 6008 6199 826 Size: 473 74 1135 681 191 1471 Fragments arranged by size: 1545 1471 1135 681 473 289 249 220 219 219 191 74 57 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6844 SmaI CCC'GGG Cuts at: 1872 4171 1872 Size: 2299 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 4948 5849 6111 820 Size: 197 926 618 2387 901 262 1553 Fragments arranged by size: 2387 1553 926 901 618 262 197 SnaBI TAC'GTA Cuts at: 493 4635 493 Size: 4142 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6844 SphI G_CATG'C Cuts at: 1132 4202 1132 Size: 3070 3774 SspI AAT'ATT Cuts at: 5 52 2396 4768 5 Size: 47 2344 2372 2081 Fragments arranged by size: 2372 2344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6844 StyI C'CwwG_G Cuts at: 513 1065 2064 3500 4283 513 Size: 552 999 1436 783 3074 Fragments arranged by size: 3074 1436 999 783 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4871 4888 5041 5226 5671 325 Size: 1255 3291 17 153 185 445 1498 Fragments arranged by size: 3291 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5090 6802 96 Size: 274 80 33 51 1443 3113 1712 138 Fragments arranged by size: 3113 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3832 3864 6804 6836 3832 Size: 32 2940 32 3840 Fragments arranged by size: 3840 2940 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6844 XbaI T'CTAG_A Cuts at: 4180 4180 Size: 6844 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6844 XmaI C'CCGG_G Cuts at: 1870 4169 1870 Size: 2299 4545 XmnI GAAnn'nnTTC Cuts at: 4973 4973 Size: 6844 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4652 276 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI