Restriction map for
pFN21AB9414
(Linear) (Six-base) MAPSORT of: KIBB9414.seq Check: 57 from: 1 to: 2139
KIBB9414.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 821 1291 1838 1897 1962 2064 2139
Size: 739 82 470 547 59 65 102 75
Fragments arranged by size:
739 547 470 102 82 75 65 59
AfeI AGC'GCT
Cuts at: 0 1944 2139
Size: 1944 195
AflII C'TTAA_G
Cuts at: 0 556 2139
Size: 556 1583
AflIII A'CryG_T
Cuts at: 0 309 2139
Size: 309 1830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1385 2139
Size: 1385 754
AlwNI CAG_nnn'CTG
Cuts at: 0 1088 1351 2139
Size: 1088 263 788
Fragments arranged by size:
1088 788 263
ApaLI G'TGCA_C
Cuts at: 0 962 1757 2139
Size: 962 795 382
Fragments arranged by size:
962 795 382
ApoI r'AATT_y
Cuts at: 0 131 2139
Size: 131 2008
BamHI G'GATC_C
Cuts at: 0 1613 2139
Size: 1613 526
BanII G_rGCy'C
Cuts at: 0 59 2139
Size: 59 2080
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 191 506 1844 2139
Size: 32 159 315 1338 295
Fragments arranged by size:
1338 315 295 159 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 2139
Size: 115 34 1990
Fragments arranged by size:
1990 115 34
Bme1580I G_kGCm'C
Cuts at: 0 43 966 1686 1761 2139
Size: 43 923 720 75 378
Fragments arranged by size:
923 720 378 75 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 1132 2139
Size: 1132 1007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1445 2139
Size: 1445 694
Bpu10I CC'TnA_GC
Cuts at: 0 1242 1678 2139
Size: 1242 436 461
Fragments arranged by size:
1242 461 436
BsaAI yAC'GTr
Cuts at: 0 310 2139
Size: 310 1829
BsaBI GATnn'nnATC
Cuts at: 0 1886 2139
Size: 1886 253
BsaWI w'CCGG_w
Cuts at: 0 491 2139
Size: 491 1648
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1305 2015 2139
Size: 1305 710 124
Fragments arranged by size:
1305 710 124
BseYI C'CCAG_C
Cuts at: 0 189 872 1063 1824 1939 2089 2139
Size: 189 683 191 761 115 150 50
Fragments arranged by size:
761 683 191 189 150 115 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1981 2139
Size: 1981 158
BsiHKAI G_wGCw'C
Cuts at: 0 966 1761 2139
Size: 966 795 378
Fragments arranged by size:
966 795 378
Bsp1286I G_dGCh'C
Cuts at: 0 43 59 966 1686 1761 2139
Size: 43 16 907 720 75 378
Fragments arranged by size:
907 720 378 75 43 16
BspHI T'CATG_A
Cuts at: 0 639 2139
Size: 639 1500
BsrBI CCG'CTC
Cuts at: 0 1450 2139
Size: 1450 689
BstYI r'GATC_y
Cuts at: 0 1613 1988 2139
Size: 1613 375 151
Fragments arranged by size:
1613 375 151
BtsI GCAGTG_nn'
Cuts at: 0 616 802 1772 2139
Size: 616 186 970 367
Fragments arranged by size:
970 616 367 186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 896 931 1695 1730 2139
Size: 896 35 764 35 409
Fragments arranged by size:
896 764 409 35 35
DraI TTT'AAA
Cuts at: 0 377 1652 2139
Size: 377 1275 487
Fragments arranged by size:
1275 487 377
EaeI y'GGCC_r
Cuts at: 0 659 2139
Size: 659 1480
EarI CTCTTCn'nnn_
Cuts at: 0 212 1123 1310 1323 1880 2139
Size: 212 911 187 13 557 259
Fragments arranged by size:
911 557 259 212 187 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 821 1291 1838 1897 1962 2064 2139
Size: 739 82 470 547 59 65 102 75
Fragments arranged by size:
739 547 470 102 82 75 65 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 977 2139
Size: 977 1162
EcoO109I rG'GnC_Cy
Cuts at: 0 1917 2139
Size: 1917 222
EcoRI G'AATT_C
Cuts at: 0 131 2139
Size: 131 2008
EcoRV GAT'ATC
Cuts at: 0 75 881 2139
Size: 75 806 1258
Fragments arranged by size:
1258 806 75
HaeII r_GCGC'y
Cuts at: 0 357 1946 1973 2139
Size: 357 1589 27 166
Fragments arranged by size:
1589 357 166 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1017 2139
Size: 985 32 1122
Fragments arranged by size:
1122 985 32
HincII GTy'rAC
Cuts at: 0 528 2139
Size: 528 1611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1766 1793 1990 2139
Size: 1766 27 197 149
Fragments arranged by size:
1766 197 149 27
MslI CAynn'nnrTG
Cuts at: 0 1927 2139
Size: 1927 212
MspA1I CmG'CkG
Cuts at: 0 1814 1995 2139
Size: 1814 181 144
Fragments arranged by size:
1814 181 144
NdeI CA'TA_TG
Cuts at: 0 444 2139
Size: 444 1695
NspI r_CATG'y
Cuts at: 0 167 633 2139
Size: 167 466 1506
Fragments arranged by size:
1506 466 167
PflMI CCAn_nnn'nTGG
Cuts at: 0 910 1088 1351 2139
Size: 910 178 263 788
Fragments arranged by size:
910 788 263 178
PsiI TTA'TAA
Cuts at: 0 200 1457 2139
Size: 200 1257 682
Fragments arranged by size:
1257 682 200
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 701 733 2139
Size: 701 32 1406
Fragments arranged by size:
1406 701 32
PstI C_TGCA'G
Cuts at: 0 589 809 1374 2139
Size: 589 220 565 765
Fragments arranged by size:
765 589 565 220
PvuII CAG'CTG
Cuts at: 0 1814 2139
Size: 1814 325
SapI GCTCTTCn'nnn_
Cuts at: 0 212 2139
Size: 212 1927
SexAI A'CCwGG_T
Cuts at: 0 909 2139
Size: 909 1230
SfcI C'TryA_G
Cuts at: 0 585 805 1094 1151 1370 1619 1640 2113
Size: 585 220 289 57 219 249 21 473
Cuts at: 2113 2139
Size: 26
Fragments arranged by size:
585 473 289 249 220 219 57 26
21
SmlI C'TyrA_G
Cuts at: 0 556 2139
Size: 556 1583
SspI AAT'ATT
Cuts at: 0 391 2139
Size: 391 1748
StyI C'CwwG_G
Cuts at: 0 59 1495 2139
Size: 59 1436 644
Fragments arranged by size:
1436 644 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1827 1859 2139
Size: 1827 32 280
Fragments arranged by size:
1827 280 32
Enzymes that do cut:
AcuI AfeI AflII AflIII AlfI AlwNI ApaLI ApoI
BamHI BanII BbsI BcgI Bme1580I BmrI BplI Bpu10I
BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrBI BstYI BtsI CspCI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
MmeI MslI MspA1I NdeI NspI PflMI PsiI PsrI
PstI PvuII SapI SexAI SfcI SmlI SspI StyI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BpmI BpuEI BsaI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI EciI
EcoICRI FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI