(Circular) (Six-base) MAPSORT of: pFN21AB9392.seq Check: 9480 from: 1 to: 7111 pFN21AB9392 7111 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3398 3398 Size: 7111 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3501 3901 4454 1937 Size: 1564 400 553 4594 Fragments arranged by size: 4594 1564 553 400 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 2895 3561 3759 3891 3915 5174 6558 Size: 887 376 666 198 132 24 1259 1384 Cuts at: 6558 6826 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1259 887 666 376 268 198 132 24 AfeI AGC'GCT Cuts at: 4280 4280 Size: 7111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 2058 6010 6987 1203 Size: 855 3952 977 1327 Fragments arranged by size: 3952 1327 977 855 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 5840 5840 Size: 7111 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AlwNI CAG_nnn'CTG Cuts at: 2234 3085 4129 4854 6426 2234 Size: 851 1044 725 1572 2919 Fragments arranged by size: 2919 1572 1044 851 725 ApaLI G'TGCA_C Cuts at: 2044 3570 5168 6324 2044 Size: 1526 1598 1156 2831 Fragments arranged by size: 2831 1598 1526 1156 ApoI r'AATT_y Cuts at: 1493 2856 3435 4168 4420 4646 4798 6907 1493 Size: 1363 579 733 252 226 152 2109 1697 Fragments arranged by size: 2109 1697 1363 733 579 252 226 152 AseI AT'TA_AT Cuts at: 160 1032 5665 160 Size: 872 4633 1606 Fragments arranged by size: 4633 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 4436 1870 Size: 73 2493 4545 Fragments arranged by size: 4545 2493 73 BamHI G'GATC_C Cuts at: 4441 4808 4441 Size: 367 6744 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4432 5887 618 Size: 325 217 627 2645 1455 1842 Fragments arranged by size: 2645 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2944 4430 1391 Size: 1553 1486 4072 Fragments arranged by size: 4072 1553 1486 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2625 2825 928 Size: 971 726 200 5214 Fragments arranged by size: 5214 971 726 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5302 5336 1903 Size: 34 3365 34 3678 Fragments arranged by size: 3678 3365 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2004 3924 5003 6213 2004 Size: 1920 1079 1210 2902 Fragments arranged by size: 2902 1920 1210 1079 BclI T'GATC_A Cuts at: 1548 2236 3583 1548 Size: 688 1347 5076 Fragments arranged by size: 5076 1347 688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 136 Size: 107 122 71 631 605 4050 1525 Fragments arranged by size: 4050 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7106 7106 Size: 7111 BlpI GC'TnA_GC Cuts at: 4528 4528 Size: 7111 Bme1580I G_kGCm'C Cuts at: 1790 2048 2356 3574 5172 6328 1790 Size: 258 308 1218 1598 1156 2573 Fragments arranged by size: 2573 1598 1218 1156 308 258 BmgBI CAC'GTC Cuts at: 1318 2043 2059 2116 1318 Size: 725 16 57 6313 Fragments arranged by size: 6313 725 57 16 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3289 3972 4842 Size: 416 53 83 399 24 1872 683 870 Cuts at: 4842 5800 442 Size: 958 1753 Fragments arranged by size: 1872 1753 958 870 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2958 5771 1239 Size: 1719 2813 2579 Fragments arranged by size: 2813 2579 1719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2812 3206 5236 6101 6399 2812 Size: 394 2030 865 298 3524 Fragments arranged by size: 3524 2030 865 394 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2926 3006 5774 882 Size: 617 1427 80 2768 2219 Fragments arranged by size: 2768 2219 1427 617 80 BsaAI yAC'GTr Cuts at: 493 1204 2483 4902 493 Size: 711 1279 2419 2702 Fragments arranged by size: 2702 2419 1279 711 BsaBI GATnn'nnATC Cuts at: 7105 7105 Size: 7111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4918 5300 5935 275 Size: 53 83 186 936 3385 382 635 1451 Fragments arranged by size: 3385 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5544 5942 6216 6363 6774 5544 Size: 398 274 147 411 5881 Fragments arranged by size: 5881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3664 3694 3883 3913 1178 Size: 30 165 30 2261 30 189 30 4376 Fragments arranged by size: 4376 2261 189 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 4074 1186 Size: 910 1978 4223 Fragments arranged by size: 4223 1978 910 BseYI C'CCAG_C Cuts at: 6314 6810 6314 Size: 496 6615 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4561 5322 6350 1540 Size: 275 186 1504 1056 761 1028 2301 Fragments arranged by size: 2301 1504 1056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 3574 5172 5257 6328 2048 Size: 1526 1598 85 1071 2831 Fragments arranged by size: 2831 1598 1526 1071 85 BsiWI C'GTAC_G Cuts at: 3308 3308 Size: 7111 BsmI GAATG_Cn' Cuts at: 2132 4622 4715 2132 Size: 2490 93 4528 Fragments arranged by size: 4528 2490 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2356 2944 3574 4430 5172 5257 Size: 399 258 308 588 630 856 742 85 Cuts at: 5257 6328 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 856 742 630 588 399 308 258 85 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 4344 5000 6730 4344 Size: 656 1730 4725 Fragments arranged by size: 4725 1730 656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3398 3862 3911 4466 844 Size: 2554 464 49 555 3489 Fragments arranged by size: 3489 2554 555 464 49 BsrBI CCG'CTC Cuts at: 1394 1823 2095 4998 1394 Size: 429 272 2903 3507 Fragments arranged by size: 3507 2903 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 5606 5780 65 Size: 1183 4358 174 1396 Fragments arranged by size: 4358 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4317 5755 5942 1700 Size: 110 2507 1438 187 2869 Fragments arranged by size: 2869 2507 1438 187 110 BsrGI T'GTAC_A Cuts at: 96 4035 96 Size: 3939 3172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 2349 2697 5171 6183 2349 Size: 348 2474 1012 3277 Fragments arranged by size: 3277 2474 1012 348 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7111 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 3923 1184 Size: 2739 4372 BstXI CCAn_nnnn'nTGG Cuts at: 3431 3431 Size: 7111 BstYI r'GATC_y Cuts at: 1972 2686 4441 4808 5193 5210 6662 6748 6760 Size: 714 1755 367 385 17 1452 86 12 Cuts at: 6760 7106 1972 Size: 346 1977 Fragments arranged by size: 1977 1755 1452 714 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3805 513 Size: 552 2740 3819 Fragments arranged by size: 3819 2740 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6372 Fragments arranged by size: 6372 683 56 BtsI GCAGTG_nn' Cuts at: 4627 5421 5441 4627 Size: 794 20 6297 Fragments arranged by size: 6297 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 3709 4415 3709 Size: 706 6405 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6118 809 Size: 507 4802 1802 Fragments arranged by size: 4802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3785 4330 4558 Size: 54 1425 50 275 18 1955 545 228 Cuts at: 4558 5447 7076 8 Size: 889 1629 43 Fragments arranged by size: 1955 1629 1425 889 545 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3147 3268 3832 5041 1353 Size: 791 1003 121 564 1209 3423 Fragments arranged by size: 3423 1209 1003 791 564 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5698 6072 6218 387 Size: 1064 138 4109 374 146 1280 Fragments arranged by size: 4109 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2895 2958 3561 3759 3891 3915 Size: 393 887 376 63 603 198 132 24 Cuts at: 3915 5174 5771 6558 6826 1239 Size: 1259 597 787 268 1524 Fragments arranged by size: 1524 1259 887 787 603 597 393 376 268 198 132 63 24 EcoNI CCTnn'n_nnAGG Cuts at: 3741 3741 Size: 7111 EcoO109I rG'GnC_Cy Cuts at: 2321 2321 Size: 7111 EcoRI G'AATT_C Cuts at: 3435 4420 4798 3435 Size: 985 378 5748 Fragments arranged by size: 5748 985 378 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 2108 2137 3826 4282 6258 1678 Size: 430 29 1689 456 1976 2531 Fragments arranged by size: 2531 1976 1689 456 430 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2462 2494 3782 Size: 32 135 32 473 32 385 32 1288 Cuts at: 3782 3814 3883 3915 5752 5784 5826 5858 1373 Size: 32 69 32 1837 32 42 32 2626 Fragments arranged by size: 2626 1837 1288 473 385 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3502 4455 4701 669 Size: 988 281 1564 953 246 3079 Fragments arranged by size: 3079 1564 988 953 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7111 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4062 4710 4062 Size: 648 6463 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2173 3349 6225 6409 1305 Size: 834 34 1176 2876 184 2007 Fragments arranged by size: 2876 2007 1176 834 184 34 MscI TGG'CCA Cuts at: 10 64 1489 1832 3787 4332 10 Size: 54 1425 343 1955 545 2789 Fragments arranged by size: 2789 1955 1425 545 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3429 5069 5428 5587 518 Size: 648 2263 1640 359 159 2042 Fragments arranged by size: 2263 2042 1640 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 3581 4527 5204 6352 6597 1606 Size: 609 1366 946 677 1148 245 2120 Fragments arranged by size: 2120 1366 1148 946 677 609 245 NaeI GCC'GGC Cuts at: 4319 4319 Size: 7111 NcoI C'CATG_G Cuts at: 513 1065 3805 513 Size: 552 2740 3819 Fragments arranged by size: 3819 2740 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NgoMIV G'CCGG_C Cuts at: 4317 4317 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 3079 4469 6014 1132 Size: 97 1850 1390 1545 2229 Fragments arranged by size: 2229 1850 1545 1390 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7111 PfoI T'CCnGG_A Cuts at: 2773 2773 Size: 7111 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PmlI CAC'GTG Cuts at: 2483 2483 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2321 2321 Size: 7111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 2760 3768 4681 2760 Size: 1008 913 5190 Fragments arranged by size: 5190 1008 913 PstI C_TGCA'G Cuts at: 830 4368 4463 830 Size: 3538 95 3478 Fragments arranged by size: 3538 3478 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 2215 3581 2215 Size: 1366 5745 SalI G'TCGA_C Cuts at: 1936 3500 4453 1936 Size: 1564 953 4594 Fragments arranged by size: 4594 1564 953 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 3262 5359 7071 3262 Size: 2097 1712 3302 Fragments arranged by size: 3302 2097 1712 SfcI C'TryA_G Cuts at: 826 1045 2907 4274 4364 4396 4459 5594 6275 Size: 219 1862 1367 90 32 63 1135 681 Cuts at: 6275 6466 826 Size: 191 1471 Fragments arranged by size: 1862 1471 1367 1135 681 219 191 90 63 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 4438 1872 Size: 2566 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2827 3221 5215 6116 6378 820 Size: 197 926 884 394 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 884 394 262 197 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SspI AAT'ATT Cuts at: 5 52 5035 5 Size: 47 4983 2081 Fragments arranged by size: 4983 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7111 StyI C'CwwG_G Cuts at: 513 1065 2422 3805 3860 4550 513 Size: 552 1357 1383 55 690 3074 Fragments arranged by size: 3074 1383 1357 690 552 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3519 5138 5155 5308 5493 5938 325 Size: 1255 1939 1619 17 153 185 445 1498 Fragments arranged by size: 1939 1619 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3260 3320 3472 Size: 274 80 33 51 1443 1283 60 152 Cuts at: 3472 4035 5357 7069 96 Size: 563 1322 1712 138 Fragments arranged by size: 1712 1443 1322 1283 563 274 152 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7071 7103 7071 Size: 32 7079 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 2668 4447 2668 Size: 1779 5332 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 4436 1870 Size: 2566 4545 XmnI GAAnn'nnTTC Cuts at: 2858 5240 2858 Size: 2382 4729 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI